Advanced Science
·
2025
·
IF 14.1
Jing Ren,Qi Wang,Xiaxia Zhang,Yongheng Cao,JingXia Wu,Juan Tian,Yanjun Yu,Qingqiu Gong,Zhaosheng Kong
Legumes establish symbiosis with rhizobia by forming a symbiotic interface that enables cross-kingdom exchanges of signaling molecules and nutrients. However, how host organelles interact with symbiosomes at the symbiotic interface remains elusive during rhizobia endosymbiosis. Here, symbiotic cells are reconstructed using 3D scanning electron microscopy (SEM) and uncover that the host endoplasmic reticulum (ER) undergoes dynamic expansion to gradually enwrap symbiosomes, facilitating their compartmentalization and endosymbiosis. Consistently, altering ER lamellar expansion by overexpressing MtRTNLBs , the reticulons responsible for ER tubulation, impairs rhizobia accommodation and symbiosome development. Intriguingly, unfolded protein response (UPR)-marker genes, bZIP60 and IRE1A/B , show continuously activated expression during nodule development, and the two UPR-deficient mutants, ire1b , and bzip60 , exhibit compromised ER biogenesis and defective symbiosome development. Collectively, the findings underpin ER expansion and UPR activation as two key events in rhizobia accommodation and reveal an intrinsic coupling of ER morphology with proper UPR during root nodule symbiosis.
研究领域:
植物学
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分子生物学
·
细胞生物学
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DOI
PubMed
高影响因子
Algal Research-Biomass Biofuels and Bioproducts
·
2023
·
IF 5.1
Yue Wang,Weihao Ning,Meina Han,Changfei Gao,Wanqian Guo,Jo-Shu Chang,Shih-Hsin Ho
The ciprofloxacin treating via microalga and its microalgae-bacteria consortium was investigated for discussing the potential application of microalgae-mediated bioremediation. As results, the maximum removal efficiency of ciprofloxacin by pure microalgae and the consortium was 87.5 ± 3.5 % (5 mg/L) and 96.1 ± 0.07 % (40 mg/L), respectively. The consortium enhanced the degradation rate to 0.81 d −1 compared with the pure microalgae (0.27 d −1 ). To reveal the symbiotic mechanism, extracellular polymeric substances , pigments contents, antioxidant enzymes and symbiotic bacterial community were analyzed. The malondialdehyde content in the pure microalgae (1622.6 ± 38.7 nmol/mg protein) was higher than that in the consortium (298.34 ± 2.4 nmol/mg protein), and there were obvious chlorophyll b accumulations (approximately 90 %) in consortium. In addition, the symbiotic bacteria had a positive effect on microalgal secretion of fulvic acid-type components. As mechanism, symbiotic bacteria improved ciprofloxacin biodegradation by reducing cell damage and secreting fulvic acid . The presence of Phycisphaeraceae and Rhizobiaceae , nitrogen fixation bacteria, in the consortium suggest that improvement of ciprofloxacin biodegradation might be associated with nitrogen co-metabolism as well. Collectively, this work elucidates the mechanism of enhancing elimination of ciprofloxacin via microalgae-bacteria consortium and accelerates development of microalgae-mediated aquaculture tailwater bioremediation.
研究领域:
环境科学
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微生物学
·
水产养殖
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药理学
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产品关联
DOI
高影响因子
TRENDS IN MICROBIOLOGY
·
2022
·
IF 18.23
Noreen Ashraf,Fiaz Ahmad,Yandu Lu
Microalga–microbiome interactions are central to both health and disease of aquatic environments. Despite impressive advances in deciphering how microorganisms participate in and impact aquatic ecosystems, the evolution and ecological involvement of microalgae and the microbiome in polluted waters are typically studied independently. Here, the phycosphere (i.e., the consortia of microalgae and the related microbiome) is regarded as an independent and integrated life form, and we summarize the survival strategies exhibited by this symbiont when exposed to anthropogenic pollution. We highlight the cellular strategies and discuss the modulation at the transcriptional and population levels, which reciprocally alters community structure or genome composition for medium-term acclimation or long-term adaptation. We propose a ‘PollutantBiome’ concept to help the understanding of microalga–microbiome interactions and development of beneficial microbial synthetic communities for pollutant remediation.
研究领域:
环境科学
·
微生物学
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DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2021
·
IF 9.642
Guanjing Cai,Gefu Zhu,Mingdian Zhou,Nan Lv,Ruming Wang,Chunxing Li,Junjie Li,Xiaofang Pan
Interspecies electron transfer (IET) between syntrophic fatty-acid oxidizing bacteria (SFOBs) and methanogens decided the performance of anaerobic digestion. Electron shuttles, as potential IET accelerators, were controversial concerning their influences on methanogenesis. In this study, concentration-dependent effects of anthraquinone-2-sulfonate (AQS) and cysteine on glucose digestion were firstly demonstrated: low dosage of AQS and cysteine (50 and 100 µM, respectively) had highest methane yield (133.5% and 148.6%, respectively). Using butyrate as substrate, distinct tendencies towards the enrichment of methanogenic community were further revealed. Cysteine just acted as a reductant which lowered ORP quickly and enriched most methanogens. It benefited methanogenesis right until methanogenic substrates accumulated. AQS, however, showed characteristic features of electron shuttles: it was firstly oxidized by SFOBs and then reduced by hydrogenotrophic methanogens, which accelerated methanogenic butyrate degradation. This study showed wide spectrum of SFOBs and methanogens benefited from the addition of electron shuttles, which laid foundation for future application.
研究领域:
环境微生物学
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厌氧消化
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生物技术
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产品关联
DOI
PubMed
高影响因子
WATER AIR AND SOIL POLLUTION
·
2021
·Vol. 233
·期 1
·1-17
·
IF 2.52
Zhang Shujuan,Yun Wenfei,Xia Yu,Wu Sikai,You Zhaoyang,Matthias C. Rillig
This study aims to investigate the role of arbuscular mycorrhizal (AM) symbiosis in reducing N loss from paddy fields, using two rice lines: a mycorrhiza-defective rice line (non-mycorrhizal) and its mycorrhizal progenitor. Two rice lines were grown in the presence of an AM fungal isolate. In this study, N loss of runoff, leaching, N 2 O emission, and NH 3 volatilization were measured, and in addition, N uptake of rice, soil aggregates, and plant available N concentration of soil. The results obtained suggest that N loss via runoff, leaching, NH 3 volatilization, and N 2 O emission of mycorrhizal rice was 11%, 8%, 6%, and 1%, lower than that of non-mycorrhizal rice, respectively. Meanwhile, mycorrhizal rice has higher biomass and plant N uptake. Our study shows that the AM symbiosis contributes to the sustainability of rice production by reducing N loss, enhancing soil aggregation and increasing plant N uptake.
研究领域:
植物学
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环境科学
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农业科学
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产品关联
DOI
高影响因子
mBio
·
2024
·Vol. 15
·期 3
·
IF 5.1
Yilin Xiao,Luyao Gao,Zhiyong Li
Global warming is a key issue that causes coral bleaching mainly because of the thermosensitivity of zooxanthellae. Compared with the well-studied zooxanthellae Symbiodiniaceae in coral holobionts, we rarely know about other coral symbiotic algae, let alone their thermal tolerance. In this study, a zoochlorellae, Symbiochlorum hainanensis, isolated from the coral Porites lutea, was proven to have a threshold temperature of 38°C. Meanwhile, unique high-temperature tolerance mechanisms were suggested by integrated transcriptomics and real-time quantitative PCR, physiological and biochemical analyses, and electron microscopy observation. Under heat stress, S. hainanensis shared some similar response strategies with zooxanthellae Effrenium sp., such as increased ascorbate peroxidase, glutathione peroxidase, superoxide dismutase activities and chlorophyll a, thiamine, and thiamine phosphate contents. In particular, more chloroplast internal layered structure, increased CAT activity, enhanced selenate reduction, and thylakoid assembly pathways were highlighted for S. hainanensis’s high-temperature tolerance. Notably, it is the first time to reveal a whole selenate reduction pathway from SeO42− to Se2− and its contribution to the high-temperature tolerance of S. hainanensis. These unique mechanisms, including antioxidation and maintaining photosynthesis homeostasis, efficiently ensure the high-temperature tolerance of S. hainanensis than Effrenium sp. Compared with the thermosensitivity of coral symbiotic zooxanthellae Symbiodiniaceae, this study provides novel insights into the high-temperature tolerance mechanisms of coral symbiotic zoochlorellae S. hainanensis, which will contribute to corals’ survival in the warming oceans caused by global climate change.
研究领域:
海洋生物学
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环境科学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2022
·Vol. 29
·期 55
·83262-83272
·
IF 5.19
Zhou Xudong,Jiang Xincheng,Chen Jibao,Gao Pengcheng
The extensive use of tetracyclines in human and veterinary medicine causes contamination in the environment that could contribute to the spread of antibiotic-resistant bacteria or competition between species of phytoplankton. In this study, Microcystis aeruginosa (a bloom-forming cyanobacterium) and Chlamydomonas microsphaera (common green alga) were selected to test the effects of different concentrations of tetracyclines (tetracycline and oxytetracycline) in monoculture and co-culture. The results showed that compared with monoculture, the cell growth of C. microsphaera decreased significantly in co-culture treated with different concentrations of tetracycline and oxytetracycline. The ratios of inhibition of M. aeruginosa exposed to 0.1, 2, and 10 mg L −1 of tetracycline varied between 17.7 and 31.37% in co-culture compared with monoculture, while the cell growth of M. aeruginosa was enhanced by treatment with 0.1, 2, and 7.25 mg L −1 of oxytetracycline in co-culture. However, the cell growth of C. microsphaera was significantly inhibited by all the treatments in co-culture. With the treatment of tetracycline, the specific growth rate of M. aeruginosa was 0.36 to 0.31 day −1 in monoculture and co-culture, while that of C. microsphaera ranged from 0.38 to 0.26 day −1 in monoculture, and it decreased from 0.25 day −1 (0 mg L −1 ) to 0.08 day −1 (10 mg L −1 ) in co-culture. With the treatment of oxytetracycline, the specific growth rate of M. aeruginosa was stimulated in co-culture, while that of C. microsphaera was significantly inhibited in co-culture compared with monoculture. Therefore, although M. aeruginosa significantly inhibited C. microsphaera in co-culture with the tetracycline-free treatment, the competitive advantage of M. aeruginosa expanded following the addition of low or high concentrations of tetracyclines.
研究领域:
环境科学
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微生物学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2023
·Vol. 25
·期 1
·386
·
IF 5.6
Ziyuan Deng,Chengling Lai,Jun Zhang,Fan Sun,Danting Li,Peiying Hao,Xuping Shentu,Kun Pang,Xiaoping Yu
The brown planthopperNilaparvata lugens(Stål) (BPH) is a main rice pest in China and many other Asian countries. In the control of BPH, the application of insect-resistant rice has proven to be quite effective. Secondary metabolites are essential weapons in plants’ defense against phytophagous insects. Studies have found that differences in the content of secondary metabolites play a crucial role in determining whether rice exhibits resistance or susceptibility to BPH. Simultaneously, symbionts are essential to the BPH. Nevertheless, there is limited research on the impact of secondary metabolites on the symbionts within BPH. Therefore, investigating the influence of secondary metabolites on both BPH and their symbionts is significant for the control of BPH. In this experiment, newly emerged female adults of BPH were fed artificial diets containing 10 different secondary metabolites. The results indicated that methyl jasmonate had inhibitory effects on the survival rate, weight gain, and reproductive capacity of BPH. Using qPCR methods, it was discovered that the number of symbiotic fungi (Ascomycetessymbionts) within BPH significantly decreased under methyl jasmonate stress. In conclusion, this experiment has preliminarily revealed the inhibitory effects of methyl jasmonate on BPH and its symbionts, demonstrating its potential for controlling BPH.Keywords:brown planthopper;secondary metabolites;symbionts;methyl jasmonate
研究领域:
植物科学
·
昆虫学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
Bioresource Technology Reports
·
2023
·
IF 0
Shaoqing Wang,Gaixiu Yang,Juntao Yang,Zhen Wan,Zhihe Li,Weiming Yi
In this paper, the ternary molten salt (Li 2 CO 3 -Na 2 CO 3 -K 2 CO 3 ) was introduced into the lignin pyrolysis process for the co-production of monophenols and value-added carbon materials. The effect of lignin/molten salt ratios (i.e., 3:1, 1:1, and 1:3) on lignin pyrolysis behaviour was investigated herein. The pyrolytic products were characterized by gas chromatography–mass spectrometry, ultimate analyses, scanning electron microscopy, nitrogen adsorption analyses, and Raman analyses. Results showed that molten salt media can promote the conversion of bulky phenolic oligomers into mono-phenols by enhancing the cracking and deoxygenation reactions. P-type phenols (49.76 %) were produced when the lignin/molten salt mass ratio was 1:3. The porosity and aromaticity of biochar were regulated in-situ using molten salt. The possible catalytic effect of molten salt on lignin pyrolysis was proposed. This study provides a case reference for lignin waste resource utilization.
研究领域:
生物质转化
·
能源化工
·
材料科学
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DOI
Insects
·
2018
·Vol. 9
·期 4
·161
·
IF 1.848
Ning Lv,Lei Wang,Wen Sang,Chang-Zhong Liu,Bao-Li Qiu
Pea aphid (Acyrthosiphon pisum) is a worldwide pest that feeds exclusively on the phloem sap of numerous host plants. It harbours a well-known primary endosymbiontBuchneraaphidicolathat helps to overcome the nutritional deficiency of a plant-based diet. However, how theBuchneracontributes to the nutritional and energy metabolism of its aphid host is unclear to date. In the current study, the function ofBuchnerain relation to nutritional synthesis of pea aphid was investigated by disrupting the primary endosymbiont with an antibiotic rifampicin. Our findings revealed that the disruption ofBuchneraled to infertility and higher loss in body mass of aphid hosts. Body length and width were also decreased significantly compared to healthy aphids. The detection of nutrition indicated that the quantity of proteins, soluble sugars, and glycogen in aposymbiotic pea aphids increased slowly with the growth of the aphid host. In comparison, the quantities of all the nutritional factors were significantly lower than those of symbiotic pea aphids, while the quantity of total lipid and neutral fat in aposymbiotic pea aphids were distinctly higher than those of symbiotic ones. Thus, we concluded that the significant reduction of the total amount of proteins, soluble sugars, and glycogen and the significant increase of neutral fats in aposymbiotic pea aphids were due to the disruption ofBuchnera, which confirmed that the function ofBuchnerais irreplaceable in the pea aphid.Keywords:Buchnera;pea aphid;endosymbiont;antibiotics;nutrition
研究领域:
昆虫学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF ECONOMIC ENTOMOLOGY
·
2025
·
IF 2.4
Tian Chao,Tang Jie,Zhu Qingyu,Guo Xiqian,Shu Qilong,Gu Zhiya,Li Fanchi,Li Bing
Bombyx mori (L.) (Lepidoptera: Bombycidae) is an important economic insect, and Exorista sorbillans (W.) (Diptera: Tachinidae) is an endoparasitic pest of larval B. mori. Dimethoate is less toxic to B. mori than E. sorbillans and is used in sericulture to controlling E. sorbillans. To investigate the effects of dimethoate treatment on the gut microorganisms and physiological functions of B. mori, 16S rRNA sequencing was used to analyzed the composition and structure of the gut microbiota. This study investigated their role in enhancing silkworm resistance by screening dominant populations after dimethoate treatment. The results indicated that dimethoate did not alter the composition of the dominant gut bacterial groups in silkworm; however, it significantly increased the abundance of the gut bacteria Methylobacterium and Aureimonas, and decreased the abundance of Enterobacterales, Bifidobacterium, Blautia, Collinsella, Faecalibacterium, and Prevotella. Eleven strains of dimethoate-resistant bacteria were selected through in vitro culture, all of which were unable to grow when dimethoate was used as a carbon source. Additionally, a germ-free silkworm model was established to assess detoxifying enzyme activity in the midgut. The results revealed that the gut symbiotic microbiota can enhance dimethoate resistance by increasing detoxification enzyme activity. This study identifies a novel pathway for silkworm resistance to dimethoate based on gut microbiota, providing new insights into the role of symbiotic gut bacteria in insecticide metabolism.
研究领域:
昆虫学
·
微生物学
·
农药科学
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产品关联
DOI
PubMed
高影响因子
Water
·
2023
·Vol. 15
·期 20
·3544
·
IF 3.4
Qingnan Sun,Xiaoping Zhang,Xin Zhang
In the treatment of wastewater containing only chemical oxygen demand (COD) byChlorellasp., the cell activity and proliferation ability ofChlorellasp. decreased with the culture time, which affected the removal of COD in wastewater. To solve these problems, theChlorellasp.–natural microorganism symbiosis system was prepared. The system was used to explore how natural microorganisms affect the cell activity and the proliferation ability ofChlorellasp. in wastewater. In the treatment of COD byChlorellasp., the removal rate of COD decreased from 45.47% to 28.88%, with a decrease in the cell activity and proliferation ability ofChlorellasp. In theChlorellasp.–natural microorganism symbiotic system, the removal rate of COD reached 45.75%. With the introduction of natural microorganisms, the circulation of CO2and O2betweenChlorellasp. and natural microorganisms promoted photosynthesis and respiration, which enhanced the cell activity ofChlorellasp. Under the condition that the dosage of natural microorganisms was between 1% and 6%, the concentration ofChlorellasp. was close to the logarithmic growth phase, which maintained the proliferation ability ofChlorellasp. At the same time, the natural microorganisms grew and proliferated in wastewater containing only COD through preying onChlorellasp.Keywords:Chlorellasp.;natural microorganisms;symbiosis system;cell activity;predation
研究领域:
环境生物学
·
废水处理
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）