FOOD HYDROCOLLOIDS
·
2025
·
IF 12.4
Yaping Liu,Chenxin Huang,Jiajie Wang,Xianchao Feng,Meihu Ma
Egg white proteins exhibit high thermal susceptibility to undesired aggregation, which limits their processing utility. In this study, the thermally regulated co-aggregation mechanism between ovotransferrin (OVT) and ovalbumin (OVA) was systematically investigated. Turbidity kinetics and particle size distribution results showed that the initial aggregation occurred at 55°C, induced by 4.3% disulfide cleavage and a 38.9% enrichment of β-sheet ( P < 0.05). Size exclusion chromatography results showed that this structural unfolding was mainly due to OVT molecules. The structurally activated OVT then specifically anchored the double cavities of OVA. Subsequently, upon heating at 60 - 65°C, the β-sheet reverted to the α-helix as detected by CD and FTIR spectroscopy, and the hydrophobic tyrosine and phenylalanine residues were reburied as detected by fluorescence spectroscopy, forming reversible metastable aggregates. At temperatures above 65°C, the larger heteroprotein complex (∼1.5 μm) emerged. ITC results showed that the co-aggregation was entropy-driven ( TΔS = +45.5 kJ/mol) with a stoichiometric ratio of 2:1 (OVT: OVA, n = 2.19 ± 0.12). Molecular docking and interaction force analyses revealed covalent disulfide bonds and multiple non-covalent bonds involved in this process. Notably, OVT acted as the aggregation initiator and governed the reverse aggregation, whereas OVA provided the structural backbone. These results provided a theoretical basis for the precise regulation of complex functional properties in the food industry.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2024
·
IF 5.3
Yuze Sheng,Xianzi Zheng,Lu Li,Haisheng He,Wei Wu,Yi Lu
Surfactants are crucial in formulating poorly soluble drugs but lead to serious side effects due to PEG chains. Novel supra-amphiphiles consisting of fatty acids and choline are developed, which spontaneously form ionic co-aggregates (ICAs) in water and exhibit strong solubilizing capacity. Paclitaxel (PTX) is adopted as a model drug here to evaluate the feasibility of choline oleate-based ICAs in the intravenous delivery of poorly soluble drugs by comparing the kinetics and distribution of payloads and nanocarriers. Choline oleate presents a maximum 10-fold enhancement in solubilizing capacity to PTX than Cremophor EL (CreEL), enabling a one-tenth use level in the formulation. Aggregation-caused quenching probes are utilized to evaluate the kinetics and biodistribution of ICAs or CreEL-based micelles (MCs). A huge gap is found between the pharmacokinetic and particokinetic curves of either nanocarrier, indicating fast leakage. ICAs lead to faster PTX leakage in blood circulation but higher PTX distribution to organs than MCs. MCs present a longer circulation in blood but a slower distribution to organs than ICAs. ICAs do not arise adverse reactions in rats following repeated injections, while MCs cause pathological changes in varying degrees. In conclusion, choline oleate-based ICAs provide an alternative to surfactants in formulating poorly soluble drugs.
研究领域:
药剂学
·
生物医学工程
·
药物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Materials Interfaces
·
2022
·Vol. 9
·期 12
·2102036
·
IF 6.389
Qi Ouyang,Qilin Gui,Chang Liu,Xiaonong Chen
Endocrine disruptors (EDCs) are widely detected in water resources owing to the abuse of plastic products, posing a serious global concern. Unfortunately, the rapid, effective, and economical removal of trace EDCs from water remains a significant challenge. Herein, a novel adsorptive membrane (PVPM) with excellent recyclability and stability is prepared by the UV-initiated grafting of poly(N-vinylpyrrolidone) (PNVP) onto the surface of polypropylene fibrous membrane (PPM). The PVPM exhibits high flux, low-pressure drop, and excellent adsorption performance. It can efficiently capture trace (10 µg L −1 ) EDCs with a removal efficiency of 99%. Notably, the adsorption performance of the PVPM remains unchanged even in river water. A mechanism for the adsorption is proposed based on adsorption kinetics and thermodynamics. Specifically, dynamic hydrophobic microdomains are generated between amphiphilic PNVP and EDCs and form co-aggregates on the PPM substrate. This co-aggregation is responsible for the large adsorption capacity for EDCs. A large decrease in the free energy (−16.65 kJ mol −1 ) of the adsorption process provides additional evidence for the strong adsorption affinity of the PVPM to EDCs. This study introduces a new pathway for designing low-cost and high-performance membrane adsorbents for prompt and efficient capture of trace EDCs from water.
研究领域:
环境科学
·
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2020
·
IF 5.339
Jing Chen,Yuzhi Wang,Xiaoxiao Wei,Ziwei Liu,Fangting Xu,Heqiong Li,Xiyan He
As mixtures, deep eutectic solvent (DES) is designability. By adjusting the long alkyl chain hydrogen bond acceptors (HBAs) or hydrogen bond donors (HBDs), the DES displays surfactant characteristics and can form micelles . Hence, a novel, simple, facile and green natural organic acids capped copper nanoclusters (Aci-CuNCs) was synthesized and the spectrum behavior of Aci-CuNCs in DES micelles was researched. It was found that the surfactant-like DES can form micellar co-aggregation with Aci-CuNCs, resulting in the fluorescence (FL) intensive of Aci-CuNCs increase. Corresponding performance of spectral properties of Aci-CuNCs in DES medium were systematically studied by fourier transform infrared spectrometer, 3D FL spectroscopy, FL emission/excitation spectra, ultraviolet absorption spectroscopy . In the mechanism exploration part, on the one hand, the existence of micellar co-aggregation was confirmed by the conductivity , the mass effect of DES, dynamic light scattering and transmission electron microscopy . On the other hand, the influence of different kinds of DESs (types of HBAs/HBDs, molar ratio) and some possible factors (ionic strength and temperature) were discussed in detail to investigate the main driving forces for the formation of micellar co-aggregates. The results of mechanism exploration prove that the long alkyl chain of DES is amphiphilic which can form micellar co-aggregation with Aci-CuNCs through hydrogen bonding . The DES micelle provides Aci-CuNCs with a relatively stable and closed micro-environment which can effectively prevent collisions with water molecules and weakening of fluorescence intensity . On the basic of the above research, a “turn-off” fluorimetric method based on Aci-CuNCs in DES medium was applied for the determination of Fe 3+ . Under the optimum conditions, the assay worked in the Fe 3+ concentration ranges from 1 to -20 μM and had a detection limit of 0.0374 μM. Method validation study illustrates the proposed system can provide a good accuracy, repeatability and stability conditions. Furthermore, the real sample analysis result demonstrates that no obvious matrix effect is found. As a consequence, the FL assays (Aci-CuNCs-based DES) composed of natural organic acid capped CuNCs and green solvent DES provides a simple, gentle and environmentally friendly method for the detection of iron ions .
研究领域:
分析化学
·
纳米技术
·
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Science
·
2025
·
IF 14.1
Jie Zhao,Xinyi Liu,Dan Li,Xuefei Jing,Weijie Wu,Liangrui He,Yizhang Tang,Zhen Liu,Yulin Zeng,Haitian Sha,Huibin Qiu,Xujiang Yu,Wanwan Li
Constructing colloidal particles into designated hierarchies with integrated functionalities offers insights into bottom-up material fabrication. However, the arbitrary and controllable assembly of individual nanoscale and microscale particles remains highly challenging due to their diverse structural properties. Here, a general paradigm for the robust co-assembly of plasmonic noble metal nanoparticles and multifunctional polymer microspheres through thermodynamically driven heterocoagulation and coordination interactions is developed. Monodisperse poly(styrene-co-maleic anhydride) microspheres (PSMA MSs) are facilely synthesized using a one-step emulsification method with optional simultaneous encapsulation of magnetic and fluorescent nanoparticles. Upon surfactant removal, the purified PSMA MSs become metastable and trigger ultrafast co-assembly of silver nanocubes (AgNCs) onto MSs within 5 min with the maximum coverage rate reaching nearly 50%. The densely immobilized AgNCs outside magnetic MSs exhibit excellent catalytic efficiency (>89% conversion across 15 cycles) and enable enzyme integration for cascade reactions. Moreover, the largest reported plasmonic-fluorescent encoded array (124 codes) is realized, achieving a significantly improved detection limit of 10 copies/reaction for respiratory viruses. This work addresses the long-standing challenge of achieving uniform distribution and dense immobilization of plasmonic noble metal nanoparticles and offers a general approach to fabricating designated hierarchies based on noble metal nanoparticles, with integrated functionalities for high-performance practical applications.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Ruihong Dong,Jinlong Tian,Ziyan Huang,Qiang Yu,Jianhua Xie,Bin Li,Chang Li,Yi Chen
This study investigated the protective effect of β-glucan (BG), konjac glucomannan (KGM) and xanthan gum (XG) on thermo-stability and antioxidant capacities of blueberry anthocyanins (ACN) and their interaction mechanisms. Twenty-six glycosylated and acylated ACN were identified, and malvidin-3- O -galactose was predominant (36.78 %) in ACN extracts. Three polysaccharides retained colour and stability and antioxidant capabilities of ACN under thermal-treatments (XG > KGM > BG). Rheological properties (shear stress, apparent viscosity) of three polysaccharides were enhanced in presence of ACN. UV–visible spectra, SEM and DLS results indicated that co-aggregation between ACN and specific zones of these polysaccharides was formed. TGA and DSC studies confirmed that introduction of three polysaccharides, especially XG could improve thermostability of ACN. FTIR, and molecular dynamics simulations revealed that thermo-stabilization of polysaccharides-ACN conjugates might be attributed to their intermolecular interactions mainly via hydrogen bindings. The protection by water-soluble polysaccharides foresees novel anthocyanins in food products with increased heat-resistant stability.
研究领域:
食品科学
·
化学
·
生物物理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）