INTERNATIONAL BIODETERIORATION & BIODEGRADATION
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2025
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IF 4.1
Qingyun Li,Shaohui Yan,Zhengfeng Jiang,Liang Xian,Jianjia Du,Haibo Liu,Youyan Liu
Cyanide and phenol are highly toxic chemicals that exert severe inhibitory effects on microorganisms, posing significant challenges for their removal by biological methods in coexistent systems. In this study, we successfully isolated a bacterial strain named JF101, identified as Alcaligenes faecalis , which was capable of degrading both cyanide and phenol. The interactions between cyanide and phenol during biodegradation were investigated. It was found that the presence of 100 mg/L cyanide significantly enhanced the degradation of phenol, leading to the complete degradation of 1000 mg/L phenol within 86 h. In contrast, only 21.9% of phenol was degraded in the absence of cyanide. Additionally, the presence of phenol had minimal impact on cyanide degradation; a concentration of 100 mg/L cyanide could be rapidly and completely degraded within 40 min even in the presence of up to 1000 mg/L phenol. Five empirical kinetic models of Haldane, Aiba, Edward, Yano, and Webb were employed to describe the degradation processes of phenol and cyanide in both single-substrate and dual-substrate systems. The putative degrading enzymes, degradation products, and pathways associated with the co-degradation of cyanide and phenol were systematically analyzed. The formamide and NH 4 + -N produced from the rapid degradation of cyanide served as nitrogen sources, facilitating the resumed growth of strain JF101 and enhancing phenol degradation.
研究领域:
环境微生物学
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污染治理
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生物化学
✓ 已验证
产品关联
DOI
高影响因子
ENVIRONMENTAL RESEARCH
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2024
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IF 7.7
Xuan Zhu,Yi-Fan Zhao,Hong-Jian Wen,Yu Lu,Shuai You,Richard Ansah Herman,Jun Wang
Silkworm pupae, by-product of sericulture industry, is massively discarded. The degradation rate of silkworm pupae protein is critical to further employment, which reduces the impact of waste on the environment. Herein, magnetic Janus mesoporous silica nanoparticles immobilized proteinase K mutant T206M and Mucor circinelloides aspartic protease were employed in the co-degradation. The thermostability of T206M improved by enhancing structural rigidity ( t 1/2 by 30 min and T 50 by 5 °C), prompting the degradation efficiency. At 65 °C and pH 7, degradation rate reached the highest of 61.7%, which improved by 26% compared with single free protease degradation. Besides, the immobilized protease is easy to separate and reuse, which maintains 50% activity after 10 recycles. Therefore, immobilized protease co-degradation was first applied to the development and utilization of silkworm pupae resulting in the release of promising antioxidant properties and reduces the environmental impact by utilizing a natural and renewable resource.
研究领域:
生物技术
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环境科学
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蛋白质工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
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2025
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IF 7.2
Mingkun Bao,Zhenhao Wang,Chaoyi Li,Tianming Li,Chaoran Fan,Na Yan,Yian Zheng
Coal gasification fine slag (CGFS) is a carbon-rich industrial solid waste featured with rough, porous surface and abundant inorganic minerals, facing significant utilization challenges. This study systematically investigated the potential of CGFS as a mineral carbon-based catalyst to activate peroxydisulfate (PDS) for degrading binary bisphenol A (BPA) and tetrabromobisphenol A (TBBPA). Under optimal conditions of 0.10 g/L PDS, 0.20 g/L CGFS, pH 7.0 and 30 °C, the CGFS-activated PDS system completely degraded binary BPA and TBBPA within 2 h, achieving a mineralization efficiency of over 53.8%. The presence of polar groups and defects in CGFS was beneficial for its adsorption, while surface moieties such as oxygen-containing functional groups and graphite carbon domains mediated PDS activation. The catalytic degradation process involved radical (•OH, SO 4 •- and O 2 •- ) and non-radical ( 1 O 2 ) pathways, which substantially contributed to the transformation of binary contaminants. Liquid chromatography-mass spectrometry identified degradation intermediates, and ECOSAR assessment indicated their lower ecotoxicity compared to the parent compounds. The practical biotoxicity of the degradation system was tested using Escherichia coli . Heat treatment effectively regenerated CGFS for reuse, and its good adaptability to actual water bodies demonstrated its great potential for practical application. This study elucidates the simultaneous degradation mechanism of BPA and TBBPA via CGFS-activated PDS system. This pioneering approach offers a cost-effective solution to treat BPA/TBBPA-containing wastewater and opens up new possibilities for CGFS recycling.
研究领域:
环境科学
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环境工程
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化学工程
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工业生态学
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污染物控制化学
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废物资源化
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催化化学
✓ 已验证
产品关联
DOI
高影响因子
BIOORGANIC CHEMISTRY
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2024
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IF 4.5
Kai Wang,Meixu Jiang,Huimin Liu,Chen Meng,Mengyuan Li,Haibin Lu
Reactivating p53 activity to restore its anticancer function is an attractive cancer treatment strategy. In this study, we designed and synthesized a series of novel PROTACs to reactivate p53 via the co-degradation of CK1α and CDK7/9 proteins. Bioactivity studies showed that the selected PROTAC 13i exhibited potency antiproliferative activity in MV4-11 (IC 50 = 0.096 ± 0.012 μM) and MOLM-13 (IC 50 = 0.072 ± 0.014 μM) cells, and induced apoptosis of MV4-11 cells. Western-blot analysis showed that PROTAC 13i triple CK1α and CDK7/9 protein degradation resulted in the significantly increased expression of p53. At the same time, the transcriptional repression due to the degradation significantly reduced downstream gene expression of MYC, MDM2, BCL-2 and MCL-1, and reduced the inflammatory cytokine levels of TNF-α, IL-1β and IL-6 in PMBCs. These results indicate the beneficial impact of simultaneous CK1α and CDK7/9 degradation for acute myeloid leukemia therapy.
研究领域:
肿瘤学
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药理学
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分子生物学
✓ 已验证
DOI
PubMed
高影响因子
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