Understanding the generation behavior of kerosene droplets is an important precursor for predicting the kerosene-mineral adsorption. Using a high-speed motion acquisition system, we investigated the thermodynamic and kinetic characteristics of kerosene droplet with different size capillaries. As the capillary inner diameter increased, the formation time of the kerosene droplet become longer, and the width/height value decreased. The width/height value of the kerosene column roughly increased as the capillary inner diameter decreased due to the variation in the buoyancy, gravity and surface tension. The kinetic energy per unit superficial area (E k /S) of the kerosene droplet firstly decreased sharply as the capillary inner diameter increased and then remained almost constant. The energy barrier on the kaolinite, quartz, montmorillonite and coal surfaces was found easy to be overcome by E k /S independently of capillary inner diameter, and the energy barrier on the mica surface could be overcome if the capillary inner diameter was smaller than 0.50 mm. A predictive model of the formation condition of deuterogenic kerosene droplet was established, and the critical capillary inner diameter for forming a deuterogenic kerosene droplet was predicted to be approximately 0.29 mm. This aspect of kerosene droplet characteristics has not been well represented in the earlier literature. The results can provide valuable insight into the development of technology for surfactant adsorption and mineral flotation.
We aimed to explore the effects of emodin on the energy metabolism of myocardial cells in rats with post-myocardial infarction (MI) heart failure (HF) and the extracellular signal-regulated kinase (ERK) pathway. The model of MI was established by ligation of the left anterior descending branch. After 4 weeks, the rats with left ventricular ejection fraction (LVEF) of ≤45% were used aspost-MI HF model animals and randomly divided into model, low-dose, middle-dose, high-dose and control groups (n=10). Low-, middle- and high-dose groups were gavaged with 20 mg/kg, 40 mg/kg and 60 mg/kg emodin daily, respectively. After administration for 14 d, the changes in LVEF, left ventricular end-systolic diameter (LVESD), left ventricular end-diastolic diameter (LVEDD) and interventricular septum thickness (IVS) were analyzed. The apoptosis rate of myocardial cells was detected by TUNEL staining. The levels of serum cardiac troponin I (cTnI) and peroxisome proliferator-activated receptor-γ coactivator-1 (PGC-1) were determined using ELISA, and the expressions of mitochondrial respiratory chain complex I protein and phosphorylated-ERK (p-ERK) in myocardial tissues were determined by Western blotting. Compared with model group, LVEDD, LVESD, apoptosis rate of myocardial cells, levels of serum cTnI and PGC-1, and expressions of complex I and p-ERK in myocardial tissues significantly decreased, while LVEF and IVS increased in low-dose, middle-dose, high-dose and control groups (P<0.05). The changes in the above indices were significantly dependent on the dose of emodin (P<0.05).Emodin can significantly relieve post-MI HF, reduce the apoptosis rate of myocardial tissues, and ameliorate the cardiac function of rats.
Immobilized metal affinity chromatography (IMAC) ensures the specific purification of proteins containing histidine tags through high affinity with transition metal chelators, which has various applications in biological protein separation. Most chromatographic separations currently use a fixed bed. In this form, internal flow pressure drops very sharply, accompanied by uneven solution flow, pore blockages, etc., all of which greatly reduce separation efficiency. Therefore, this study uses hollow fiber membranes (HFMs) with micron-scale inner diameters as a base, thus reducing operating pressure and significantly enhancing mass transmission. Batch adsorption experiments were performed using flat plate membranes to obtain the reaction's thermodynamic and kinetic model parameters for use in a dynamic column breakthrough simulation. The numerical simulation was based on a single HFM model and established a mathematical model for computational fluid dynamics (CFD) in ANSYS Fluent software. Model accuracy was validated by combining the simulation with experiments. The effects of different module and process parameters on the breakthrough curve were investigated by varying parameters such as flow rate, initial feed concentration, and HFM inner diameter. Design parameters and operating conditions contributing to module utilization were subsequently obtained.
危险品化学品经营许可证（带存储）