Frontiers in Pharmacology
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2022
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IF 5.988
Chen Ze-Yan,Wei Xu-Ya,Qiu Zi-Dong,Huang Yun,Tan Ting,Feng Yu-Lin,Guo Juan,Cui Guang-Hong,Huang Lu-Qi,Lai Chang-Jiang-Sheng
The herb-pair ginseng-Fuzi (the root of Aconitum carmichaelii) is the material basis of Shenfu prescriptions and is popular in traditional Chinese medicine for the treatment of heart failure, and even shock with severe-stage of COVID-19. A narrow therapeutic window of Fuzi may cause significant regional loss of property and life in clinics. Therefore, systemic elucidation of active components is crucial to improve the safety dose window of Shenfu oral prescriptions. A high performance liquid chromatography-mass spectrometry method was developed for quantification of 10 aconitines in SD rat plasma within 9 min. The limit of detection and the limit of quantification were below 0.032 ng/ml and 0.095 ng/ml, respectively. Furthermore, a systemic comparison with their pharmacokinetic characteristics after oral administration of a safe dosage of 2 g/kg of Fuzi and ginseng-Fuzi decoction for 24 h was conducted. Eight representative diester, monoester, and non-ester aconitines and two new active components (i.e., songorine and indaconitine) were all adopted to elucidating the differences of the pharmacokinetic parameters in vivo. The compatibility of Fuzi and ginseng could significantly increase the in vivo exposure of active components. The terminal elimination half-life and the area under the concentration-time curve of mesaconitine, benzoylaconitine, benzoylmesaconitine, benzoylhypaconitine, and songorine were all increased significantly. The hypaconitine, benzoylmesaconitine, and songorine were regarded as the main active components in vivo, which gave an effective clue for the development of new Shenfu oral prescriptions.
研究领域:
中药学
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药理学
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分析化学
✓ 已验证
DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
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2015
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IF 4.099
Lifang Zhou,Yajing Lei,Dai Zhang,Shabbir Ahmed,Shuqing Chen
Dibutyl phthalate (DBP) has been extensively used as a plasticizer in many daily products, which is highly toxic to human, notably affecting the reproductive and developmental function. As the previous method is expensive, time-consuming, low sensitivity and just focused on the environment. Present study was aimed to establish an ultra-sensitive and simple method based on good quality monoclonal antibody, applying to evaluate excretion level of DBP in urine samples of Chinese population directly. A monoclonal antibody was generated and characterized after fusion of myeloma cells with spleen cells isolated from BALB/c mouse. The mouse was previously immunized using a specially designed amino derivative of DBP conjugated with bovine serum albumin (BSA) as immunogen. Cross-reactivity values of the monoclonal antibody against DBP, di-isobutyl phthalate (DIBP) were observed 100% and 1.25%, while for dimethyl phthalate (DMP), butyl benzyl phthalate (BBP) and didecyl phthalate (DDP) the values were < 0.06%. The standard curve was constructed at 0–50 ng mL − 1 and good linearity (R 2 = 0.994) was achieved. The observed IC 50 (7.34 ng mL − 1 ) and LOD (0.06 ng mL − 1 ) values was improved 1000-fold to polyclonal antibody and 5-fold to other monoclonal antibodies. A total 1246 urine samples were analyzed and the detection frequency of DBP was observed 72.87% by ic-ELISA. The 95th percentile and mean concentration of DBP were 12.07 and 3.00 ng mL − 1 . Acceptable recovery rates of DBP were 97.8–114.3% and coefficients variation 5.93–11.09%. The concentrations of DBP in females were found significantly higher (p < 0.05) than males. Similarly, the DBP in middle aged and low educated individuals was found higher (p < 0.001) than the others. Considering the adverse health effects, DBP internal exposure in the Chinese population should be reduced. The ic-ELISA method has been proved as a cost effective, specific, and highly sensitive screening tool to detect DBP in urinary samples.
研究领域:
毒理学
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环境健康
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TOXICOLOGY LETTERS
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2022
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IF 4.271
Sen Yan,Sinuo Tian,Zhiyuan Meng,Jin Yan,Ming Jia,Wei Sun,Qiuxia Wang,Jinling Diao,Wentao Zhu,Zhiqiang Zhou
Decabromodiphenyl ethane (DBDPE) as a novel brominated flame retardant is frequently detected in environmental media due to its widespread use. Studies have shown that exposure to environmental pollutants in utero could lead to weight loss in newborns and obesity in adulthood. However, the mechanisms of how the cubs grow rapidly from low birth weight to obesity remain unclear. Although it has been reported that perinatal DBDPE exposure caused obesity in the offspring of mice in adulthood, its metabolic changes in offspring juvenile are unknown. Here, we monitored changes of body weight in cubs following exposure to DBDPE in utero. Furthermore, 1 H NMR-based metabolomics was used to investigate the effects of in utero DBDPE exposure on the metabolic profile of neonates included days 1 and 25. Additionally, the expression levels of key genes in the relevant pathways were quantified. The results showed that exposure to high levels of DBDPE in utero caused cubs to be smaller at birth and grow rapidly during lactation. Metabolomics analysis showed that cubs in the exposed group consistently accelerated glycogenolysis and lipid synthesis to promote appetite and energy absorption, and achieved rapid growth in the subsequent lactation, which was further evidenced by the expression levels of genes related to glycolipid metabolism. These results reveal the mechanism for the first time how the weight loss of newborns in utero environmental pollutant exposure transformed into obesity in adulthood, although the exposures here were much higher than would be anticipated from the environment.
研究领域:
毒理学
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环境科学
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代谢生物学
✓ 已验证
DOI
PubMed
高影响因子
TALANTA
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2017
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IF 4.162
Yu Zhang,Qiao Wang,Gong Zhang,Wei Jia,Yiping Ren,Yongning Wu
Hemoglobin (Hb) adducts of acrylamide (AA) and its oxidative metabolite glycidamide (GA) are important biomarkers for evaluating the mid-term exposure of acrylamide toxicity in vivo. Taking pentafluoro-2-methylphenyl isothiocyanates of N-(2-carbamoylethyl)valine (AAVal-PFPTH) and N-(2-carbamoyl-2-hydroxyethy)valine (GAVal-PFPTH) as target analytes, we developed an isotope dilution ultra-high performance liquid chromatograph tandem mass spectrometry (UHPLC-MS/MS) method for the simultaneous determination of AA and GA hemoglobin (Hb) adducts under the electroscopy ionization negative (ESI‾) mode in the present work. Among them, the enantiomer pair of GA-Hb adducts was firstly identified and successfully separated at baseline level. The method achieved high sensitivity with the LOD and LOQ ranging 1.43–5.05 pmol/g Hb and 4.78–16.82 pmol/g Hb, respectively. The recovery rates with low, intermediate and high spiking levels were calculated as 97.0–105.2%, 97.4–106.4% and 100.3–111.2%, respectively. Acceptable within-laboratory reproducibility (RSD < 13.7%) substantially supported the robustness of current UHPLC-MS/MS method, which was successfully applied to measure the hemoglobin adducts of acrylamide and glycidamide enantiomers in blood of both rats and humans. A linear exposure assessment model was developed for estimating the daily exposure to acrylamide in humans via considering acrylamide hemoglobin adducts as variables, indicating a novel connect between biomarker-based internal exposure and dietary-based external exposure. Overall, the present instrumental analysis and related internal exposure assessment model provide a substantially methodological support for profiling the internal biological exposure and estimating the external dietary exposure to acrylamide.
研究领域:
毒理学
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分析化学
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公共卫生
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2025
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IF 11.3
Kaiming Yang,Yiwei Gong,Guanlan Wu,Jiawen Zhang,Weici Quan,Yuxi Wu,Yadi Ai,Di Huang,Kai Yang,Hongguang Cheng,Qingrui Zhang,How Yong Ng
Liquid crystal monomers (LCMs), which are the key material of liquid crystal displays, have been recently considered as potential persistent, bioaccumulative, and toxic (PBT) chemicals. However, there is limited information on the accumulation and metabolism of LCMs in humans. In this study, a total of 162 paired serum and urine samples of a general population (aged 19 - 70 years) residing in Northwest China were collected and analyzed for the concentrations of 15 representative LCMs. The mean ∑15LCMs concentrations were 129.13 and 38.52 ng/mL in the serum and urine, respectively. BEEB was found to be the most P&B LCM and dominated both serum and urine. DFPrB accumulated remarkably in the serum, while PBIPHCN and BEEB were more easily eliminated by the kidneys. The strong correlations of some LCMs in the paired serum and urine suggest that urinary LCM concentrations can reflect their metabolic levels in the body. The estimated daily intake of ∑15LCMs for the study participants based on the serum data was 3.41 ng/kg body weight/day, and the hazard index of ∑15LCMs was 5.23×10 -5 which is acceptable (< 1). This study provides the evidence for the presence of LCMs in the serum and urine of general population and highlights the renal clearance of LCMs in the human body.
研究领域:
环境健康
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毒理学
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环境科学
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人体暴露评估
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）