Foods
·
2024
·Vol. 13
·期 2
·261
·
IF 4.7
Shanshan Liu,Mingyue Shen,Jianhua Xie,Bohan Liu,Chang Li
2-Monochloropropane-1, 3-diol (2-MCPD) esters and 3-monochloropropane-1,2-diol (3-MCPD) esters, a class of substances potentially harmful to human health, are usually formed during the refining of vegetable oils under high temperature. The effects of endogenous antioxidants in vegetable oils on the formation of 2- and 3-MCPD esters is still unknown. In this study, the effects of endogenous antioxidants (α-tocopherol, stigmasterol and squalene) on the formation of 2- and 3-MCPD esters in model thermal processing of camellia oil were investigated. The possible formation mechanism of 2- and 3-MCPD esters was also studied through the monitoring of acyloxonium ions, the intermediate ions of 2- and 3-MCPD esters formation, and free radicals by employing infrared spectra and electron paramagnetic resonance (EPR), respectively. The results indicated that the addition of α-tocopherol had either promoting or inhibiting effects on the formation of 2- and 3-MCPD esters, depending on the amount added. Stigmasterol inhibited the formation of 3-MCPD ester and 2-MCPD ester at low concentrations, while promoting their formation at high concentrations. Squalene exhibited a promotional effect on the formation of 3-MCPD ester and 2-MCPD ester, with an increased promotion effect as the amount of squalene added increased. The EPR results suggested that CCl3•, Lipid alkoxyl, N3• and SO3• formed during the processing of camellia oil, which may further mediate the formation of chlorpropanol esters. This study also inferred that squalene promotes the participation of the free radical in chlorpropanol ester formation.Keywords:MCPD esters;endogenous antioxidants;camellia oil;mechanism
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2019
·Vol. 11
·期 13
·1751-1756
·
IF 2.378
Kangming Xiong,Fangjun Huo,Yongbin Zhang,Ying Wen,Caixia Yin
Hypochlorous acid (HClO), as one of the reactive oxygen species (ROS), is very important to the human immune system. Although many hypochlorous acid fluorescent probes have been developed, most of them are not suitable for detecting endogenous HClO due to their own limitations such as a slow response time and short emission wavelength. In this work, we designed a near-infrared ratiometric (2.1 to 15.3, I608/I735) fluorescent probe with a quick response (20 s) based on cyanine polyene derivatives. The system could detect endogenous hypochlorous acid in HepG-2 cells. It is significant that the color change in the measurement process makes the probe applicable to the determination of hypochlorous acid content in an 84 disinfectant and other actual samples.
研究领域:
生物化学
·
医学检测
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2020
·
IF 6.551
Runyuan Zhang,Xiaoxian Zheng,Dongqing Zhang,Xiaojun Niu,Jinlin Ma,Zhang Lin,Mingli Fu,Shaoqi Zhou
Owing to its environmental-friendliness, low-cost, and outstanding characteristics, biochar has been widely used for the catalytic degradation of various organic pollutants. In this study, a pre- and post-deashing graphitized biochar (DBC800 and PBC800-A) was prepared and compared with the pristine biochar (PBC800) to activate persulfate (PS) for tetracycline (TC) degradation. The influence of the natural endogenous mineral on the catalytic ability of biochar was investigated. Characterization results show that the inherent endogenous mineral in biochar not only acted as a natural pore-forming agent to promote the formation of the porous structure, but also facilitated the formation of edge defective structures, and altered the surface functional groups, as well as increased the carbonization and graphitization degree of biochar. The PBC800-A exhibited a much higher catalytic efficiency on PS activation and TC oxidative degradation with the reaction rate of 0.06055 min −1 , 7.14 times as that of DBC800 (0.00861 min −1 ) and 4.63 times as that of PBC800 (0.00158 min −1 ). The endogenous minerals were conducive to the generation of free radicals and promoted the oxidative degradation of TC, which was mainly attributed to the improved carbon configuration. The post-deashing treatment was also found to significantly improve the electron transport efficiency of biochar by removing the residual ash, thereby promoting the generation of singlet oxygen. This study demonstrated that the natural minerals in biochar was beneficial for the degradation of TC, and more alternative natural minerals can be applied to co-pyrolysis with biochar for the remediation of refractory organic pollutants.
研究领域:
环境科学
·
污染控制
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Kai Wang,Rui Guo,Xu-Yang Chen,Xiu-Lin Li,Zhi-Gang Hu,Xiao Wang,Chao-Yue Wang,Ya-Juan Qin,Kun Yao,Yu-Shun Yang
Hydrogen sulfide (H 2 S) performs essential functions in several physiological and pathological procedures, such as liver cirrhosis, diabetes mellitus, and cancer. To optimize the H 2 S detection in practical applications, herein, a new probe YG-H 2 S for the fluorescent imaging of H 2 S was developed. Compared with the current H 2 S probes, the main advantages of YG-H 2 S were a wide pH range (5.0–9.0), a relatively fast response (within 10 min), and high selectivity from the competitive species (including biological mercaptan). Owing to the low cytotoxicity of YG-H 2 S , it was good for the biological depth imaging in biological events. YG-H 2 S could detect endogenous H 2 S in HeLa cells. In addition, YG-H 2 S could monitor the level of H 2 S in 4 T1-xenograft and acute liver injury model mice as well as in separate tissues. Consequently, YG-H 2 S might be a potential method for detecting H 2 S in preliminary diagnosis.
研究领域:
生物化学
·
医学影像
·
病理学
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DOI
高影响因子
NEUROPSYCHOPHARMACOLOGY
·
2021
·Vol. 47
·期 3
·652-663
·
IF 7.855
Manz Kevin M.,Coleman Benjamin C.,Jameson Alexis N.,Ghose Dipanwita G.,Patel Sachin,Grueter Brad A.
Parvalbumin-expressing fast-spiking interneurons (PV-INs) within feedforward microcircuits in the nucleus accumbens (NAc) coordinate goal-directed motivational behavior. Feedforward inhibition of medium spiny projection neurons (MSNs) is initiated by glutamatergic input from corticolimbic brain structures. While corticolimbic synapses onto MSNs are targeted by the psychostimulant, cocaine, it remains unknown whether cocaine also exerts acute neuromodulatory actions at collateralizing synapses onto PV-INs. Using whole-cell patch-clamp electrophysiology, optogenetics, and pharmacological tools in transgenic reporter mice, we found that cocaine decreases thalamocortical glutamatergic drive onto PV-INs by engaging a monoamine-independent mechanism. This mechanism relies on postsynaptic sigma-1 (σ 1 ) activity, leading to the mobilization of intracellular Ca 2+ stores that trigger retrograde endocannabinoid signaling at presynaptic type-1 cannabinoid receptors (CB 1 R). Cocaine-evoked CB 1 R activity occludes the expression of CB 1 R-dependent long-term depression (LTD) at this synaptic locus. These findings provide evidence that acute cocaine exposure targets feedforward microcircuits in the NAc and extend existing models of cocaine action on mesolimbic reward circuits.
研究领域:
神经科学
·
药理学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2021
·
IF 3.205
Xuechen Liu,Jingchen Wang,Bingying Hu,Pan Yan,Shuailong Jia,Zhifeng Du,Hongliang Jiang
Sphingolipids (SLs) are endogenously bioactive molecules with diverse structures, and its metabolic disorders are involved in the progression of many diseases. In this study, an ultra-performance liquid chromatography quadrupole exactive mass spectrometry (UPLC-Q-Exactive-MS) method was established to comprehensively profile SLs in plasma. First, the fragment patterns of SL standards of each subclass were investigated. Then, the SL species in plasma were characterized based on the fragmentation rules. Finally, a total of 144 endogenous SL species consisting of 216 regioisomers were identified in plasma of human, golden hamster and C57BL/6 mice, which was the most comprehensive identification for SLs in plasma. In addition to the known species, 19 SL species that have never been reported were also identified. The profile of SLs in plasma of human and two rodent species was compared subsequently. It was worth noting that a total of 9 SL molecular species consisting of 11 regioisomers with low abundance were successfully identified in human plasma through comparison among species. Those findings contribute to a deeper understanding of SLs in human plasma and provide scientific basis for the selection of animal model. The established profile of SLs in plasma could be used for screening of lipid biomarkers of various diseases.
研究领域:
生物化学
·
医学
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
Scientific Reports
·
2017
·Vol. 7
·期 1
·1-8
·
IF 4.259
Li Dong-Peng,Wang Zhao-Yang,Cui Jie,Wang Xin,Miao Jun-Ying,Zhao Bao-Xiang
A new ratiometric fluorescent probe was constructed with hemicyanine and 7-nitrobenzofurazan for detection of sulfur dioxide derivatives (HSO 3 − /SO 3 2− ). The ratiometric response mode could be attributed to the efficient FRET (Förster resonance energy transfer) platform. The probe exbihited some desirable properties including fast response (within 2 minutes), good selectivity and high sensitivity. Moreover, the probe could detect endogenous HSO 3 − in liver cancer cells rather than normal liver cells, implying the diagnosal potential of the probe.
研究领域:
生物化学
·
医学诊断
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
Journal of Advanced Research
·
2025
·
IF 13
Yuepeng Yin,Mengfan Jia,Ping Li,Kaiwen Zhu,Min Nie,Xin Tang,Liqin He,Ting Li,Zhihong Guo,Zhigao Zhou,Lirong Li,Taolin Zhang,Xingxiang Wang,Changfeng Ding
Introduction Traditionally, Silicon (Si) fertilizers are applied externally to the soil or sprayed on the leaves to reduce rice’s arsenic (As) accumulation. However, in practical agricultural production, the economic benefits of lowering As in rice grains often fail to outweigh the production costs. Objectives This study investigated an innovative technology—Si-rich seedlings (endogenous Si)—that is simple to implement, cost-effective, and highly effective in reducing As accumulation in rice grains. Methods The effects of exogenous and endogenous Si supplementation on As dynamics in rice were investigated in hydroponics in field experiments. Results Both methods significantly reduced As accumulation in the rice grains (22.23 % and 17.70 %, respectively). There was no significant difference in the treatment effects. However, endogenous Si supplementation demonstrated a significantly lower cost per unit of As reduction (88.61 % decrease), and it was easier to implement when compared with exogenous Si supplementation. The main processes by which various Si treatment techniques decrease As accumulation in rice differed, even though Si was crucial to As absorption in rice through a combination of mechanisms, such as gene regulation, iron plaque inhibition, and node I sequestration. The fundamental mechanism was Si/As antagonism, which regulated Si absorption through gene regulation in both methods. Interestingly, the exogenous Si treatment improved the barrier function of the root surface iron plaque against As. At the same time, laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) demonstrated that endogenous Si had a more pronounced impact on rice node I. Furthermore, experiments conducted at multiple scales (including variety, pot, and field experiments) validated the stability and reliability of the Si-rich seedling technology under complex environmental conditions. Conclusion Si-rich seedlings provide a cost-effective, stable, and practical solution for mitigating As contamination in paddy fields. This approach holds significant potential for enhancing soil health and improving food safety, contributing to the sustainable development of rice cultivation.
研究领域:
植物学
·
环境科学
·
农业科学
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产品关联
DOI
PubMed
高影响因子
POLYMER TESTING
·
2022
·
IF 4.931
Yan-Chan Wei,Wen-Yi Xie,Meng-Fan He,Ding Zhu,Shuang Liu,Ling Zhang,Shuangquan Liao
Natural rubber (NR) with complex compositions has more excellent thermo-oxidative resistance properties compared to synthetic high cis -1,4-polyisoprene (IR), although synthetic IR owns similar molecular chain structure to NR. Compared with synthetic IR, the existence of non-rubber components is an obvious characteristic of NR. In this study, we pay attention to the non-rubber components acting as endogenous antioxidants, causing significant impact on thermo-oxidative resistance of NR. After removal of non-rubber components, the retention of tensile strength of NR exhibits a 2.7-fold decrease (from 84.29% to 31.29%) after 24 h of aging at 100 °C. The effect of non-rubber components on improving thermo-oxidative resistance of NR can be verified and mimicked through additional acetone extract or δ-tocopherol. The results show that the retention of tensile strength of purified NR (PNR) increases from 3.06% to 64.31% and retention of elongation at break of PNR increases from 60.61% to 92.59% after 48 h of aging after adding 0.6% δ-tocopherol, which can be comparable to or even exceed some traditional antioxidants. Furthermore, the antioxidative mechanism of endogenous antioxidant is explored. This work reveals another characteristic of non-rubber components and provides a rational design for the new biomimetic rubber with unique compositions and excellent properties similar to NR.
研究领域:
材料科学
·
高分子化学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Guoyan Liu,Wenqi Zhu,Sitong Li,Wanli Zhou,Huijuan Zhang,Jing Wang,Xiaofang Liu,Jixian Zhang,Li Liang,Xin Xu
Endogenous phenols play a significant role in delaying oil rancidity. In this study, the profile of 22 endogenous phenols was determined from tea seed oil by UPLC-MS/MS, of which 15 phenols were identified for the first time. Then seven phenols with high content and strong antioxidant capacity were selected to investigate interaction using the DPPH· and Rancimat. It was found that the interaction of combinations was inconsistent in different media. Combined quercetin + esculetin, caffeoyl tartaric acid + esculetin, caffeoyl tartaric acid + gentisic acid and esculetin + gentisic acid showed synergistic antioxidant effects in oil and ethanol systems. Moreover, through the evaluation of the lipid oxidation process, combined esculetin + gentisic acid exhibited the greatest synergistic antioxidant effect. Notably, combined quercetin + esculetin had an inhibitory effect on the formation of volatile compounds. These findings may provide a basis for explaining the oxidation stability of tea seed oil.
研究领域:
食品科学
·
植物化学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Wei Jia,Xin Wang,Lin Shi
Endogenous hydrocortisone causes detrimental effects on public health and domestic animal products, but the potential mechanisms remain elusive. Hydrocortisone was detected from seventy-two Guanzhong-Black pigs in three replicates (216 samples) (0.00 ± 46.38 μg kg −1 ), indicating the existence of endogenous hydrocortisone. Herein, we investigated the effects of hydrocortisone on the metabolic signatures in pork via integrative metabolomics and proteomics by UHPLC-Q-Orbitrap HRMS. Animal-derived foods under hydrocortisone-bioaccumulation cause metabolic perturbation by regulating glutamine synthetase expression at the transcriptional level contributed to hydrocortisone-induced toxicity and accelerating the down-regulation of essential amino acids ( l -Histidine 10.74–6.48 mg kg −1 , l -Phenylalanine 3.70–1.57 mg kg −1 ), purine nucleotides (GMP 40.29–5.00 μg kg −1 , etc) that provide nutritional value in a positive feedback loop. Protein-metabolite interactions suggesting that hydrocortisone enhanced nitric oxide synthase and GMP reductase expression (LOQ 3.24–63.39 μg kg −1 ), and affected meat flavor perception, eventually lead to the decline of nutritional value and flesh quality of pork.
研究领域:
动物学
·
食品科学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Foods
·
2022
·Vol. 11
·期 21
·3339
·
IF 5.561
Xu Zhang,Xinshe Li,Yunhao Zhao,Qiang Wu,Yong Wan,Yougui Yu
Endogenous peptides in Chinese baijiu have been recently reported. However, little information is available on their correlation with the main flavor substances. One hundred and forty-six peptides, consisting of more bitter amino acids and key amino acids responsible for bioactivity, were identified in tail liquor using liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). Additionally, the content of endogenous peptides increased gradually with distillation time, showing a high negative correlation with total esters (r= −0.929) and total alcohol (r= −0.964) but presented a moderate positive correlation with the total acid content (r= 0.714). The results of the correlation analysis between them were further proved by molecular docking, which showed that these endogenous peptides in baijiu interacted with the main flavor substances via hydrogen bonds. This study clarifies the dynamic changes of endogenous peptides during distillation and provides a theoretical reference for the relationship between these peptides and the main flavor substances.Keywords:soy sauce aroma style baijiu;endogenous peptide;flavor substance;correlation;molecular docking
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）