SENSORS AND ACTUATORS B-CHEMICAL
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2018
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IF 5.667
Juan Ou-Yang,Wen-Li Jiang,Kai-Yue Tan,Hong-Wen Liu,Song-Jiao Li,Juan Liu,Yong-Fei Li,Chun-Yan Li
Hydrogen sulfide (H 2 S) is involved in various pathological and physiological processes as well as regulation of lysosomal functions. Thus, it is essential to develop precise method for the detection of H 2 S in lysosome. Although a large number of fluorescent probes for H 2 S have been developed, the probe for lysosomal H 2 S assay still remains rare. Herein, a two-photon fluorescent probe, named Lyso-Nap, is designed and synthesized for detecting H 2 S in lysosome by employing a dual lock system (dual PET process and dual reaction sites). The fluorescence of Lyso-Nap with dual lock system is opened by H 2 S only when the probe is localized within acidic lososomes, which can avoid the disturbance from neutral cytosol and other organelles. Moreover, two-photon fluorescent probes with weak auto-fluorescence, large penetration depth and reduced photodamage, are particularly favored for bioimaging. Accordingly, Lyso-Nap can be successfully used for precisely tracking and detecting endogenous H 2 S in lysosome of living cells and tissue.
研究领域:
生物化学
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细胞生物学
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医学检测
✓ 已验证
DOI
高影响因子
POSTHARVEST BIOLOGY AND TECHNOLOGY
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2025
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IF 6.8
Saiying Han,Ling Liu,Lilei Wang,Jin Han,Yeru Ai,Huali Wang,Kaifang Zeng,Jian Ming,Lili Deng
Prunus salicina ‘Wushan plum’ is a climacteric fruit, which rapidly ripens under the action of ethylene after harvesting, resulting in quality deterioration that seriously restricts the sustainable development of the industry. In order to examine the potential role of sodium hydrosulfide (NaHS, the donor of exogenous H 2 S) in slowing down the ripening and senescence process of plum fruit, the changes in storage quality, antioxidant capacity, endogenous signals and gene expression levels were evaluated during storage at 25 °C for twelve days. The results indicated that NaHS treatment delayed the coloration of plum fruit and maintained higher firmness, titratable acid content (TA) and marketable fruit rate. Treatment with NaHS increased the activities of catalase (CAT), superoxide dismutase (SOD), glutathione reductase (GR) and ascorbate peroxidase (APX); promoted the accumulation of total phenols (TP) and ascorbic acid (AsA), and inhibited the increase in reactive oxygen species (ROS), malondialdehyde (MDA) and lipoxygenase (LOX) activity, which in turn alleviated oxidative damage. H 2 S treatment down-regulated the expression of PsACO1 and PsACS1 and inhibited the production of ethylene; up-regulated the expression of PsSiR , PsSAT , PsOASTL, and PsLCD , which in turn elevated the activities of sulfite reductase (SiR), serine acetyltransferase (SAT), O-acetylserine cleavage lyase (OASTL), and L-cysteine desulfurylase (LCD), and facilitated the accumulation of the endogenous H 2 S content; up-regulated the expression of L-Arginine synthesis-related genes and PsNOA , which increased the content of L-Arginine as well as the activity of nitric oxide synthase (NOS) and facilitated the synthesis of nitric oxide (NO). The above results indicated that NaHS could improve the antioxidant system, endogenous H 2 S, ethylene, and NO metabolism of plum, thus maintaining the storage quality of the fruit.
研究领域:
植物生理学
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食品科学
✓ 已验证
DOI
高影响因子
CERAMICS INTERNATIONAL
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2023
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IF 5.2
Xiao Zhang,Qianhui Wei,Hongbin Zhao,Feng Wei,Tengfei Li
Hydrogen sulfide (H 2 S) is considered to be the third endogenous gas messenger after nitric oxide and carbon monoxide , and is closely related to physiological and pathological functions of human being. Rapid and real-time monitoring of endogenous H 2 S production in living cells is of great significance for clinical diagnoses of related disease and in-depth investigation of pathological processes. The application of electrochemical sensors has become a promising method to achieve real-time H 2 S monitoring comparing to other methods. Up to now, low-cost, and highly sensitive electrocatalysts for H 2 S detection are conspicuously scarce. Herein, we developed a high-efficient method to construct a H 2 S electrochemical sensor, which configure dissolved–H 2 S–sensitive electrochemical sensor using porous Pt-modified copper oxide (Pt–CuO) as electrocatalyst. The obtained sensor possessed remarkable H 2 S detection performance, achieving the detection limit of 0.5 ppm and apparent selectivity toward various gases (e.g., ammonia, nitrogen dioxide , and carbon dioxide). The electrochemical sensor was also applied for monitoring H 2 S released from mesenchymal stem cells. The enhanced sensing performance was attributed to: (1) electrocatalytic reaction between CuO and [H 2 S] (H 2 S and HS − ) was observed in the cyclic voltammetry curves with oxidation current peaks; (2) Pt catalytic activity promoted electron transfer during the detection process and further decreasing Pt–CuO resistance; and (3) enhanced diffusion of detection molecules/ions, which increased the reactivity sites through forming cross porous structures using sodium carbonate as sacrifice template. The proposed Pt–CuO-based electrochemical sensor shows great potential for applications in clinical diagnoses, and provides effective means to understand the role of H 2 S in biological processes.
研究领域:
生物医学工程
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分析化学
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生物化学
✓ 已验证
产品关联
DOI
高影响因子
Theranostics
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2020
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IF 8.579
Qingqing Tian,Lu An,Qiwei Tian,Jiaomin Lin,Shiping Yang
Rationale : Chemodynamic therapy (CDT) based on the Fe(II)-mediated Fenton reaction is an emerging tumor treatment strategy. However, the catalytic efficiency in tumors is crucially limited by Fe(II). Herein, an endogenous hydrogen sulfide (H 2 S) accelerated Fe(III)/Fe(II) transformation and photothermal synergistically enhanced CDT strategy based on ellagic acid-Fe-bovine serum albumin (EA-Fe@BSA) nanoparticles (NPs) was developed for colon tumor inhibition. On the one hand, the Fe(III) with low catalytic activity in the EA-Fe@BSA NPs could be rapidly reduced to the highly active Fe(II) by the abundant H 2 S in colon cancer tissues. Thus, a rapid Fe(III)/Fe(II) conversion system was established, wherein highly active Fe(II) ions were continuously regenerated to improve the CDT efficiency. On the other hand, the photothermal effect of EA-Fe@BSA NPs also accelerated the production of hydroxyl radicals (•OH), thereby synergistically enhancing the CDT performance and improving the therapeutic efficacy. Methods : The endogenous H 2 S accelerated Fe(III)/Fe(II) conversion and PTT enhanced CDT were investigated by characterization of the Fe valence state and detection of •OH. T 1 -weighted magnetic resonance imaging (MRI) was tested both in vitro and in vivo . The biocompatibility of NPs were examined via MTT assay, hemolysis analysis and routine blood measurements. The enhanced CDT was investigated in HCT116 colon cancer cells by Calcein-AM/PI staining and MTT assay, and tumor inhibition was demonstrated in HCT116 tumor bearing mice. Results : In this work, EA-Fe@BSA NPs were constructed as a CDT theranostic reagent. The H 2 S accelerated Fe(III)/Fe(II) conversion was confirmed, more degradation of MB and generation of •OH demonstrated the enhanced CDT in vitro . EA-Fe@BSA NPs exhibited good T 1 -weighted MRI performance. More importantly, it displayed strong near-infrared (NIR) absorption and excellent photothermal efficiency, further promotes the production of •OH. Hence, the efficacy of CDT was enhanced, and the tumor growth was inhibited efficiently. Conclusion : All results demonstrate that this strategy based on endogenous H 2 S promoted Fe(III)/Fe(II) transformation together with PTT acceleration permits efficient Fenton-reaction- mediated CDT both in vitro and in vivo , which holds great potential for effective colon cancer theranostics.
研究领域:
生物医学工程
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肿瘤治疗
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纳米医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2022
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IF 9.221
Kai Wang,Xue Yang,Meng-Ya Guo,Xu-Yang Chen,Ting Li,Ru Yan,Yu-Shun Yang,Hai-Liang Zhu,Zhi-Gang Hu
Hydrogen sulfide (H 2 S) is a signal molecule that is significantly involved in cell protection, vascular regulation, and neuro-transmission. Nevertheless, abnormal levels of H 2 S are intimately relevant to Alzheimer’s disease, liver cirrhosis, diabetes mellitus, and Down syndrome. To improve the H 2 S detection in vitro and in vivo , we developed RDM-H 2 S , a novel red-emission fluorescent probe . RDM-H 2 S has a rapid response (120 s), high sensitivity (LOD = 310 nM), obvious response degree (234 folds), relatively long excitation/emission wavelength (565 nm/620 nm), and steady emission in pH 6–9. In addition, RDM-H 2 S showed high specificity to H 2 S in various species. Due to the low cytotoxicity of RDM-H 2 S , it was beneficial to the application of biological depth imaging . In addition, RDM-H 2 S could measure exogenous and endogenous H 2 S in distinct biological models, such as HeLa cells and zebrafish. Besides, RDM-H 2 S had excellent mitochondrial targeting ability and could be used to monitor H 2 S in living mice. RDM-H 2 S could visualize endogenous H 2 S in different tissues and 4T1-xenograft tumor mice. Additionally, RDM-H 2 S also exhibited immense application potential for the visualization of H 2 S in the process of inflammation. RDM-H 2 S was applied for measuring the level of H 2 S in human plasma samples. Consequently, RDM-H 2 S was determined to be a valuable tool for monitoring H 2 S in pre-clinical diagnosis.
研究领域:
生物化学
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医学诊断
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分子生物学
✓ 已验证
DOI
高影响因子
TOXICOLOGY
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2021
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IF 4.221
Bobo Yang,Wenjun Zhao,Changsheng Yin,Yu Bai,Suhua Wang,Guangwei Xing,Fang Li,Jinsong Bian,Michael Aschner,Jiyang Cai,Haifeng Shi,Rongzhu Lu
Hydrogen sulfide (H 2 S) as the third gasotransmitter molecule serves various biological regulatory roles in health and disease. Acrylonitrile (AN) is a common occupational toxicant and environmental pollutant, causing brain and liver damage in mammals. The biotransformation of AN is dependent-upon reduced glutathione (GSH), cysteine and other sulfur-containing compounds. However, the effects of AN on the endogenous H 2 S biosynthesis pathway have yet to be determined. Herein, we demonstrated that a single exposure to AN (at 25, 50, or 75 mg/kg for 1, 6 or 24 h) decreased the endogenous H 2 S content and H 2 S-producing capacity in a dose-dependent manner, both in the cerebral cortex and liver of rats in vivo . In addition, the inhibitory effects of AN (1, 2.5, 5, 10 mM for 12 h) on the H 2 S content and/or the expression of H 2 S-producing enzymes were also found both in primary rat astrocytes and rat liver cell line (BRL cells). Impairment in the H 2 S biosynthesis pathway was also assessed in primary rat astrocytes treated with AN. It was found that inhibition of the cystathionine-β-synthase (CBS)/3-mercaptopyruvate sulfurtransferase (3-MPST)-H 2 S pathway with the CBS inhibitor or 3-MPST-targeted siRNA significantly increased the AN-induced (5 mM for 12 h) cytotoxicity in astrocytes. In turn, CBS activation or 3-MPST overexpression as well as exogenous NaHS supplementation significantly attenuated AN-induced cytotoxicity. Taken together, endogenous H 2 S biosynthesis pathway was disrupted in rats acutely exposed to AN, which contributes to acute AN neurotoxicity in primary rat astrocytes.
研究领域:
毒理学
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神经科学
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药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nano Today
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2022
·
IF 18.962
Kai Yuan,Kai Huang,Yiqi Yang,Yixuan Lin,Yihao Liu,Fupeng Li,Yakun Liang,Haishuang Chang,Yuhui Chen,Tingting Tang,Shengbing Yang
Increasing evidence has emphasized the pivotal role of antibiotic tolerance in the evolution of antibiotic resistance , refractory infection and infection recurrence. Moreover, satisfactory treatment of infection relies highly on early, precise detection and subsequent timely therapeutic interventions. In this study, the phenotype of increased production of H 2 S in antibiotic-tolerant Staphylococcus aureus ( S. aureus ) was identified and proven to be essential to the maintenance of antibiotic tolerance. Hence, a photoacoustic probe for detecting infection as well as an antibiotic enhancer targeting bacterial endogenous H 2 S to eradicate antibiotic-tolerant bacteria was successfully developed based on a bismuth metal-organic framework (Bi-MOF). Bi-MOF achieved early in situ and precise photoacoustic imaging of infection by generating Bi 2 S 3 inside bacteria. Moreover, Bi-MOF enhanced the eradication efficacy of methicillin against tolerant S. aureus by downregulating the intracellular H 2 S levels. Overall, Bi-MOF offers a promising diagnostic tool for early, precise infection diagnosis and a therapeutic strategy for tolerant bacteria-related refractory infection.
研究领域:
纳米技术
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医学诊断
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抗菌治疗
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传染病
✓ 已验证
产品关联
DOI
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2025
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IF 19
Yi Zhang,Hao Guo,Gang Wang,Fang Lan,Shiyong Zhang
The core challenge of H 2 S therapy is to supply H 2 S in a continuous and steady way. By self-polymerizing lipoic acid trisulfide, herein a new H 2 S donor called poly-lipoic acid trisulfide particles (pLATP) is developed with the steady H 2 S supply over 48 h at both cellular and animal levels, the longest time reported so far. Mechanistically, the pLATP high-efficiently enters the cytoplasm via the poly(trisulfides) mediated membrane penetration. In cells, thanks to the polymerized solid sphere structure, the pLATP releases H 2 S by a layer-by-layer decomposed way. Only when the outer layer decomposes, the inner layer can be exposed. After H 2 S release, the pLATP degrades to lipoic acid, which is not only a B vitamin, but also enhances the expression of 3-mercaptopyruvate sulfurtransferase, a H 2 S synthesizing enzyme promoting the production of endogenous H 2 S. The dual pathways of exogenous supplementation and endogenous recovery warrant the continuous and steady supply of H 2 S at the lesion site. As a proof of concept, the pLATP normalized cardiac function in rats with myocardial ischemia-reperfusion injury.
研究领域:
药理学
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生物化学
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纳米医学
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心脏病学
·
高分子化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）