JOURNAL OF PLANT GROWTH REGULATION
·
2019
·Vol. 39
·期 2
·631-640
·
IF 2.179
Jia Chunhua,Yu Xiaojing,Zhang Min,Liu Zhiguang,Zou Peng,Ma Jun,Xu Yachun
The growth and development of cold-season plants are susceptible to high temperature. Melatonin is a plant growth regulator with potential to improve plant tolerance to biological and abiotic stresses. In our study, cherry radish ( Raphanus sativus L. var. radculus pers ) was cultured at a high temperature (35 °C/30 °C day/night) and different concentrations (0, 11.6, 17.4, 29.0, 34.8 and 67.0 mg L −1 ) of melatonin were applied to these high-temperature stressed plants to its effects on biomass, quality, antioxidant enzyme activity, chlorophyll and endogenous hormone contents. The plants were grown under normal temperature (25 °C/20 °C) as control and high-temperature condition as HT-stress treatment. The results revealed that under high temperature with 29.0 mg L −1 melatonin treatment, cherry radish biomass was significantly increased by 12.9%, and the soluble protein and soluble solid were increased by 18.7 and 9.2%, respectively. The activity of antioxidant enzyme, ascorbate peroxidase and peroxidase were increased by 43.7 and 45.5%, respectively. The chlorophyll a and carotenoid contents were increased by 7.4 and 20.0% compared with the control at 27 days. The auxin and abscisic acid contents were significantly increased by 28.5 and 6.7% compared with HT at 9 days. Thus, application of optimal rate of melatonin had a positive effect on cherry radish growth under high-temperature stress.
研究领域:
植物学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2021
·
IF 3.205
Jing-Wen Hao,Xiao-Quan Liu,Yong-Jun Zang,Nai-Dong Chen,An-ling Zhu,Lu-Fan Li,Min-Zhu Shi
A rapid pressurized capillary electrochromatography (pCEC) method has been successfully developed for the simultaneous determination of 16 phytohormones in Dendrobium huoshanense . Effects of wavelength, mobile phase, the flow rate, pH value, concentration of buffer and applied voltage were investigated, respectively. The results showed that the 16 phytohormones could be baseline-separated rapidly in less than 21 min on a reversed-phase EP-100–20/45–3-C18 capillary column (total length of 45 cm, effective length of 20 cm, diameter of 100 μm, ODS packing inside for 3 μm) with ACN/5.0 mM ammonium acetate (containing 0.05% formic acid, pH = 3) as the mobile phase using gradient elution mode as follows: 0.1–10.0 min 40%ACN,10–15.0 min 70%ACN, 15.0–20 min 80% ACN, 20–21.0 min 80% ACN at a flow rate of 0.12 mL/min, applied voltage of −5 kV and a UV detection wavelength of 210 nm. The method validation howed that the established method is precise and stability, and the RSDs of intra- and inter-day precision based retention time and peak area were all below 5%. Employed the established method, in our experimental conditions, total 6 endogenous hormones including IAA, IBA, NAA, GA, ABA, t-Z were detected in D. huoshense . However, a relative larger amount of exogenous hormone 2,4-D (25.3 ~ 4.2 μg/kg) and 6-BA (79.5 ~ 35.4 μg/kg) were detected in 1 ~ 4 year old cultivated D. huoshense , suggesting there were still a certain amount of exogenous hormone residue in tissue-cultured D. huoshanese though they had been transplanted to field cultivation from the test-tube plantlets for several years.
研究领域:
植物学
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2024
·Vol. 25
·期 17
·9456
·
IF 4.9
Xinru Hou,Kewen Zhang,Yingmin Lyu
Oriental hybrid lilies, known for their vibrant colors, diverse flower shapes, and long blooming seasons, require annual bulb propagation in horticultural production. This necessity can lead to higher production costs and limit their use in landscaping. The LA hybrid lily ‘Aladdin’ has shown strong self-reproduction capabilities in optimal cultivation environments, producing numerous high-quality underground stem bulblets. This makes it a valuable model for studying bulblet formation in lilies under natural conditions. Through transcriptome data analysis of different developmental stages of ‘Aladdin’ bulblets, theLaLBD37gene, linked to bulblet formation, was identified. Bioinformatics analysis, subcellular localization studies, and transcriptional activation activity tests were conducted to understand the characteristics ofLaLBD37. By introducing theLaLBD37gene into ‘Sorbonne’ aseptic seedlings via Agrobacterium-mediated transformation, resistant plants were obtained. Positive plants were identified through various methods such as GUS activity detection, PCR, and fluorescence quantitative PCR. Phenotypic changes in positive plants were observed, and various physiological indicators were measured to confirm the role ofLaLBD37in bulblet formation, including soluble sugar content, starch content, sucrose synthase activity, and endogenous hormone levels. The findings suggest that theLaLBD37gene plays a significant role in promoting the development of lily bulblets, offering insights for enhancing the reproductive capacity of Oriental hybrid lilies and exploring the molecular mechanisms involved in lily bulb regeneration.
研究领域:
植物生物学
·
分子遗传学
·
园艺学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）