MYCOBIOLOGY
·
2017
·
IF 0.761
Qiang Zhang,Yujuan Li,Fangxue Xu,Mengmeng Zheng,Xiaozhi Xi,Xuelan Zhang,Chunchao Han
Different endophytes isolated from the seeds of Sophora flavescens were tested for their ability to produce matrine production. Response surface methodology (RSM) was applied to optimize the medium components for the endophytic fungus. Results indicated that endophyte Aspergillus terreus had the ability to produce matrine. The single factor tests demonstrated that potato starch was the best carbon source and the combination of peptone and NH 4 NO 3 was the optimal nitrogen source for A. terreus . The model of RSM predicted to gain the maximal matrine production at 20.67 µg/L, when the potato starch was 160.68 g/L, peptone was 24.96 g/L and NH 4 NO 3 was 2.11 g/L. When cultured in the optimal medium, the matrine yield was an average of 20.63 ± 0.11 µg/L, which was consistent with the model prediction. This study offered an alternative source for the matrine production by endophytic fungus fermentation and may have far-reaching prospect and value.
研究领域:
微生物学
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天然产物化学
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DOI
PubMed
高影响因子
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2013
·Vol. 97
·期 21
·9365-9375
·
IF 3.689
Pu Xiang,Qu Xixing,Chen Fei,Bao Jinku,Zhang Guolin,Luo Yinggang
Camptothecin (CPT), the third largest anticancer drug, is produced mainly by Camptotheca acuminata and Nothapodytes foetida . CPT itself is the starting material for clinical CPT-type drugs, but the plant-derived CPT cannot support the heavy demand from the global market. Research efforts have been made to identify novel sources for CPT. In this study, three CPT-producing endophytic fungi, Aspergillus sp. LY341, Aspergillus sp. LY355, and Trichoderma atroviride LY357, were isolated and identified from C. acuminata . Most CPT produced by these fungi was found in the fermentation broth, and their corresponding CPT yields were 7.93, 42.92, and 197.82 μg l −1 , respectively. The CPT-producing capability of LY341 and LY355 was completely lost after repeat subculturing. A substantial decrease of CPT production was also observed in the second generation of LY357. However, a stable and sustainable production of CPT was found from the second generation through the eighth generation of LY357. The fermentation medium, time, pH, temperature, and agitation rate were optimized for CPT production. Methyl jasmonate and XAD16 were proven to be an optimum elicitor and adsorbent resin, respectively, in view of that CPT yield was increased 3.4- and 11-fold through their use. A 50- to 75-fold increase of CPT yield was obtained when the optimized fermentation conditions, elicitor, and adsorbent resin were combined and applied to the culture of the seventh and eighth generations of LY357, and the highest CPT yield was 142.15 μg l −1 . The CPT-producing T. atroviride LY357 paves a potential to uncover the mysteries of CPT biosynthesis.
研究领域:
药用植物学
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微生物学
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药物开发
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DOI
PubMed
高影响因子
Microorganisms
·
2025
·Vol. 13
·期 5
·1103
·
IF 4.2
Yuwei Xing,Cong Liu,Xumeng Cui,Haonan Lv,Jun Wang
Nymphaea candida Presl is a rare hardy water lily at risk of extinction and has been included on the ‘Red List’ of threatened species of the International Union for the Conservation of Nature. To protect germplasm resources and propagate N. candida seedlings, this study conducted tissue culture and found that pathogenic endophytic fungal infection was the main reason for failure of tissue culture. Compared with the stems and leaves, the roots of N. candida had the highest rates of fungal infection during tissue culture. Subsequently, three isolated endophytic fungi, Fusarium oxysporum , Phytopythium helicoides , and Alternaria sp., showed the highest frequency of occurrence in tissue culture. Furthermore, an antifungal formulation comprising 0.1 μg/mL pyrimidin suspension, 1 μg/mL mancozeb wettable powder, and 1 μg/mL carbendazim was constructed and could reduce the infection rates of root and stem tissues to 7.5% and 0%, respectively. Finally, the usefulness of this antifungal formulation for inhibition of endophytic fungi in tissue culture of N. candida was validated. This study not only provides important technical support for mass production of seedlings of N. candida , but also provides a scientific reference for the protection of endangered aquatic plant species.
研究领域:
植物学
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保护生物学
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微生物学
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产品关联
DOI
PubMed
高影响因子
BioTech
·
2025
·Vol. 14
·期 1
·18
·
IF 3.1
Yao Lu,Qiang Jiang,Yamin Dong,Runzhen Ji,Yiwen Xiao,Du Zhu,Boliang Gao
The GH43 family of glycosidases represents an important class of industrial enzymes that are widely utilized across the food, pharmaceutical, and various other sectors. In this study, we identified a GH43 family glycoside hydrolytic enzyme,Xyaf313, derived from the plant endophytic fungusChaetomium globosumDX-THS3, which is capable of transforming several common ginsenosides. The enzyme function analysis reveals that Xyaf313 exhibits dual functionality, displaying both α-L-arabinofuranosidase and β-D-xylosidase activity. When acting as an α-L-arabinofuranosidase, Xyaf313 achieves optimal enzyme activity of 23.96 U/mg at a temperature of 50 °C and a pH of 7. In contrast, its β-D-xylosidase activity results in a slight reduction in enzyme activity to 23.24 U/mg, with similar optimal temperature and pH conditions to those observed for the α-L-arabinofuranosidase activity. Furthermore, Xyaf313 demonstrates considerable resistance to most metal ions and common chemical reagents. Notably, while the maximum enzyme activity of Xyaf313 occurs at 50 °C, it maintains high activity at room temperature (30 °C), with relative enzyme activity exceeding 90%. Measurements of ginsenoside transformation show that Xyaf313 can convert common ginsenosides Rc, Rb1, Rb2, and Rb3into Rd, underscoring its potential for pharmaceutical applications. Overall, our findings contribute to the identification of a new class of bifunctional GH43 glycoside hydrolases, highlight the significance of plant endophytic fungi as a promising resource for the screening of carbohydrate-decomposing enzymes, and present new candidate enzymes for the biotransformation of ginsenosides.
研究领域:
生物化学
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工业酶学
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药用植物学
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产品关联
DOI
PubMed
高影响因子
Frontiers in Plant Science
·
2022
·
IF 6.627
Shi Xia,Fang Jiaxing,Du Huicong,Zhang Sufang,Liu Fu,Zhang Zhen,Kong Xiangbo
When Ips bark beetles invade and colonize the host plants, their associated pathogenic fungal partners are carried into the phloem of the host trees. Host trees are lethally attacked by the beetle-fungus complex and the collective damage severely limits forestry production worldwide. It is of great importance to verify whether bark beetles and their associated fungi show concordant performance in terms of biology, physiology, and biochemistry on host trees. In this study, the two Ips bark beetles Ips typographus and Ips subelongatus (Coleoptera: Curculionidae, Scolytinae), their respective associated pathogenic fungi Endoconidiophora polonica and Endoconidiophora fujiensis, and their respective host plants Picea jezoensis and Larix olgensis were selected as test material. Cross-inoculation experiments were conducted indoors and outdoors to investigate the differences in reproduction and development of two beetles and infectivity of two fungi on two plants, as well as the differences in physiological responses of two plants to two fungal infections. The results showed that I. typographus and E. polonica had excellent host performance on P. jezoensis; however, neither successfully colonized and infected L. olgensis. In contrast, I. subelongatus and E. fujiensis showed strong host suitability on L. olgensis and some degree of suitability on P. jezoensis, although the host suitability of P. jezoensis for E. polonica was significantly higher than that for E. fujiensis. In addition, we found that the absolute amount of ergosterol accumulated on the lesion was positively correlated with lesion area. The ergosterol amount and lesion area were both strongly correlated with the release of host monoterpenes, but had no obvious correlation with the concentration of fungi-induced phenols on the lesion area and the side-chain oxidation of lignin in the xylem of the infected sites. Based on these results, we confirmed that “I. typographus-E. polonica” and “I. subelongatus-E. fujiensis” complexes both showed the most suitable consistent performances on their own traditional hosts, establishing a stable species-specific association relationship in these two beetle-fungus complexes, with the “I. subelongatus-E. fujiensis” complex showing broader host suitability. From the perspective of physiological responses of plants to fungal infections, monoterpenes are an important indicator of host suitability.
研究领域:
昆虫学
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植物病理学
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森林生态学
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产品关联
DOI
PubMed
高影响因子
Marine Drugs
·
2023
·Vol. 21
·期 5
·270
·
IF 5.4
Zhuling Shao,Yingying Tian,Shan Liu,Xiao Chu,Wenjun Mao
Marine microorganisms often produce exopolysaccharides with novel structures and diverse biological activities due to their specific marine environment. The novel active exopolysaccharides from marine microorganisms have become an important research area in new drug discovery, and show enormous development prospects. In the present study, a homogeneous exopolysaccharide from the fermented broth of the mangrove endophytic fungusPenicillium janthinellumN29, designated as PJ1-1, was obtained. The results of chemical and spectroscopic analyses showed that PJ1-1 was a novel galactomannan with a molecular weight of about 10.24 kDa. The backbone of PJ1-1 was composed of →2)-α-d-Manp-(1→, →4)-α-d-Manp-(1→, →3)-β-d-Galf-(1→ and →2)-β-d-Galf-(1→ units with partial glycosylation at C-3 of →2)-β-d-Galf-(1→ unit. PJ1-1 had a strong hypoglycemic activity in vitro, evaluated using the assay of α-glucosidase inhibition. The anti-diabetic effect of PJ1-1 in vivo was further investigated using mice with type 2 diabetes mellitus induced by a high-fat diet and streptozotocin. The results indicated that PJ1-1 markedly reduced blood glucose level and improved glucose tolerance. Notably, PJ1-1 increased insulin sensitivity and ameliorated insulin resistance. Moreover, PJ1-1 significantly decreased the levels of serum total cholesterol, triglyceride and low-density lipoprotein cholesterol, enhanced the level of serum high-density lipoprotein cholesterol and alleviated dyslipidemia. These results revealed that PJ1-1 could be a potential source of anti-diabetic agent.Keywords:mangrove endophytic fungus;exopolysaccharide;structure;anti-diabetic activity
研究领域:
海洋生物学
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药理学
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生物化学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Wenjun Zhang,Shuoyue Yuan,Ruoxin Chen,Pengzhen Li,Qinyu Huang,Zhenlong Zhou,Penghui Fan,Jianyu Su
Exopolysaccharides derived from endophytic fungi inhabiting medicinal plants are promising bioactive agents. Herein, a neutral exopolysaccharide termed DEPS was extracted and purified from an endophytic fungus isolated from Cinnamomum burmannii leaves, with a yield of 51.12 %. Structural analysis by HPGPC, UV, FTIR, NMR, and SEM revealed that DEPS is primarily composed of glucose, with minor amounts of mannose and galactose, and features →6)-α- d -Glcp-(1→ and →4)-α- d -Glcp-(1→ linkages. Functionally, DEPS exhibited a superior moisture-retention rate of 26.4 %, comparable to sodium alginate and glycerol, and showed moderate antioxidant activity. In vitro , DEPS effectively suppressed IL-6 expression in macrophages by approximately twofold, and inhibited zebrafish neutrophil migration by 50 %. In an acetaminophen (APAP)-induced liver injury model, DEPS treatment significantly alleviated hepatic damage by decreasing necrosis area from 55.2 % to 24.1 %, and reducing IL-1β expression from 273.3 % to 118.6 %. Additionally, DEPS promoted an anti-inflammatory environment through enhanced IL-10 expression and reduced TNF-α levels. These findings highlight DEPS as a structurally unique, multifunctional polysaccharide with hepatoprotective, anti-inflammatory, and antioxidant potential for food, pharmaceutical, and cosmetic applications.
研究领域:
微生物学
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生物化学
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药理学
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天然产物化学
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免疫学
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产品关联
DOI
PubMed
高影响因子
Journal of Fungi
·
2025
·Vol. 11
·期 3
·209
·
IF 4
Jiao Yao,Sai Huang,Lingfeng He,Shengyun Wei,Wei Yang,Qiangxin Zhang,Weihua Wang,Xiaoqin Yang,Sida Xie,Yunxian Li,Ping Zhao,Guolei Zhu
One novel C10polyacetylene rhamnoside, 4,6,8-decatriyne-1-O-α-L-rhamnopyranoside, named xylariside A (1), together with two novel C10polyacetylene quinovopyranosides, 4,6,8-decatriyne-1-O-α-D-quinovopyranoside, xylariside B (2), and 8E-decaene-4,6-diyne-1-O-α-D-quinovopyranoside, xylariside C (3), were obtained from the solid fermentation ofXylariasp. VDL4, an endophytic fungus isolated fromVaccinium dunalianumwight (Ericaceae). Their chemical structures were elucidated through a combination of spectroscopic techniques. The antifungal activities of these compounds were evaluated in vitro against four phytopathogenic fungi (Fusarium oxysporum,Botrytis cinerea,Phytophthora capsici, andFusarium solani). Compound2demonstrated significant antifungal activities, with minimum inhibitory concentration (MIC) values ranging from 3.91 to 7.81 μg/mL. Compound2’s effectiveness levels were similar to those of the reference drugs thiabendazole and carbendazim (each MIC = 0.98−15.62 μg/mL). Xylariside B (2) was further evaluated againstB. cinereain vivo. It exhibited remarkable efficacy in both the prevention and treatment of tomato and strawberry gray mold. Molecular docking studies confirmed the antifungal mechanism of compound2by revealing its binding interactions with key enzyme targets inB. cinerea, thereby supporting the observed in vitro and in vivo results. Additionally, compound2showed effective inhibition of α-glucosidase, with IC50values of 5.27 ± 0.0125 μg/mL.
研究领域:
植物化学
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药理学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2019
·
IF 3.571
Zhibo Hu,Yiwen Tao,Xingyu Tao,Qinhua Su,Jiachun Cai,Can Qin,Weijia Ding,Chunyuan Li
A new monosesquiterpene diacetylgliocladic acid (1), a new dimeric sesquiterpene divirensol H (9), and two exceptionally novel trimeric sesquiterpene trivirensols A and B (11 and 12), together with another eight known congeners, were purified from an endophytic fungus Trichoderma virens FY06, derived from Litchi chinensis Sonn. whose fruit is a delicious and popular food. All of them were identified by comprehensive spectroscopic analysis, combined with biosynthetic considerations. Trivirensols A and B are unprecedented trimers of which three subunits are connected by two ester bonds of the sesquiterpene class. Relative to the positive control triadimefon, all the tested metabolites showed strong inhibitory activities against at least one phytopathogenic fungus among Penicillium italicum, Fusarium oxysporum, Fusarium graminearum, Colletotrichum musae, and Colletotrictum gloeosporioides. Notably, as metabolites of the endophytic fungus from L. chinensis, they all presented strong antifungal activities against C. gloeosporioides which causes anthracnose in L. chinensis.
研究领域:
植物化学
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微生物学
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药物化学
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产品关联
DOI
PubMed
高影响因子
BIOORGANIC CHEMISTRY
·
2020
·
IF 4.831
Zhibo Hu,Zhihui Wu,Qinhua Su,Mingzhe Li,Shanshan Wu,Rongqi Meng,Weijia Ding,Chunyuan Li
To check whether the endophytic fungus from the host plant could produce novel bioactive metabolites induced by the host-derived chemical components, a strategy called “Induction of Endophyte Metabolism by Adding Host Components” (IEMAHC) was put up and applied. Cultivation of the endophytic fungus Botryosphaeria ramosa L29 derived from Myoporum bontioides after adding (2 R , 3 R )-3, 5, 7-trihydroxyflavanone 3-acetate, a constituent of the same plant, into the culture medium, led to the separation of three new chromones , 5-hydroxy-2, 3-dihydroxymethyl-7-methoxychromone ( 1 ), 5-hydroxy-3-acetoxymethyl-2-methyl-7- methoxychromone ( 2 ), 5, 7-dihydroxy-3-hydroxymethyl-2-methylchromone ( 3 ), one new isocoumarin , 8-hydroxy-3-hydroxymethyl-6-methoxy-7-methylisocoumarin ( 4 ), two new δ -lactones, botryopyrone ( 8 ) and (5 S , 8 R )-simplicilopyrone ( 9 ), one new naturally rare 2 (7 H )-oxepinone, botroxepinone ( 10) , and three known compounds ( 5 – 7 ). They were identified through comprehensive analysis of spectroscopic data. HPLC comparative analysis indicated that compounds 8 – 10 were induced products after adding (2 R , 3 R )-3, 5, 7-trihydroxyflavanone 3-acetate as an inducer. Compound 3 exhibited more potent inhibitory activities on Fusarium oxysporum , Fusarium graminearum , Penicillium italicum , and Colletotrichum musae than triadimefon. Compounds 1 , 2 , 4 – 7 and 9 displayed potent antifungal activities towards two or three tested fungi with MIC values equal or superior to triadimefon. The induced products 9 and 10 both showing strong inhibitory activity against C. musae , might play a role in strengthening the host defense against the common mangrove invasive pathogenic fungal genera Colletotrichum .
研究领域:
微生物学
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植物化学
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药理学
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DOI
高影响因子
Microbial Cell Factories
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2025
·Vol. 24
·期 1
·1-14
·
IF 4.9
Ma Saijian,An Chao,Xue Wenjiao,Liu Chen,Ding Hao,Zhang Qiwen,Shi Xinwei,Zhang Jingjing,Liu Yao,Shao Jingjing
Background The use of biocatalysis technology to manufacture rare natural products can solve the contradiction between the market demand for rare natural products in large health industry fields and the protection and sustainable development of wildlife resources. However, the currently available research on fungal endophytes, which are great potential resources for glycoside hydrolase biocatalysts, is still insufficient. In this study, endophytic fungi from Epimedium brevicornum Maxim. were isolated in the Qinling Mountains, identified and tested for their potential to biotransform epimedium extracts into minor epimedium flavonoids. Results A total of 84 representative morphotype strains were isolated and identified via ITS rDNA sequence analyses and were grouped into 32 taxa. The Shannon‒Wiener index ( H ’, 3.089) indicated that E. brevicornum Maxim. harboured abundant fungal resources. Ten strains showed strong β-glucosidase activity and exhibited the ability to biotransform major epimedium flavonoids into deglycosylated minor epimedium flavonoids, such as baohuoside I and icaritin, via various glycoside-hydrolysing pathways. Among these strains, strains 8509 and F8889, which were initially characterized as Aspergillus ochraceus and A. protuberus , have the potential for further development in the biotransformation of epimedium extracts into minor epimedium flavonoids because of their excellent biosafety, enzyme activity, and enzymatic characteristics. The enzyme activity of the crude enzyme obtained by freeze-drying from the F8509 fermentation broth supernatant reached 78.24 ± 2.48 U/g. Further research revealed that major glycosylated flavonoids from 100 g/L epimedium extracts were bio-transformed completely into minor deglycosylated flavonoids in 90 min after the addition of 1 g/L crude enzyme. In addition, the liquid phase separation conditions were optimized, and ethyl alcohol and water were ultimately used as the mobile phase for efficient separation of the conversion products at equal flow degrees. Conclusions This study not only identified a series of candidates for the biotransformation of minor epimedium flavonoids but also provided an efficient purification method. More importantly, this study also demonstrated the important value of endophytes in the biotransformation of rare natural products.
研究领域:
植物学
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微生物学
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药用植物研究
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生物技术
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产品关联
DOI
PubMed
高影响因子
Natural Product Communications
·
2021
·
IF 0.986
Jia-Cheng Ji,Pan-Pan Wei,Xiao-Yang Han,Zheng-Hui Li,Hong-Lian Ai,Xin-Xiang Lei
A new steroid, chaetglotone (1), together with 3 known compounds (2-4), were isolated from Chaetomium globosum, which is an endophytic fungus isolated from the root of Coptis chinensis Franch. The new compound was characterized by one-dimensional and two-dimensional nuclear magnetic resonance spectroscopy and high-resolution electrospray ionization mass spectrometer. The relative configuration and absolute configuration of 1 were further determined via the DP4 + and Early Childhood Development protocols, separately.
研究领域:
天然产物化学
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微生物学
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药学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）