Environmental Science & Technology Letters
·
2021
·
IF 7.653
Wenjuan Zhang,Yue Gao,Cheng Chen,Aijing Li,Xuerui Ma,Yong Liang,Xinglei Yao,Maoyong Song,Guibin Jiang
Bisphenol S (BPS) is a bisphenol A (BPA) replacement that is widely present in the environment and in humans. The association between obesity and BPS has been reported in epidemiological studies; however, the obesogenic effect and related mechanism of BPS remain poorly understood. In this study, high-resolution microcomputed tomography analysis showed that the volume of visceral fat was significantly increased in BPS-treated mice. Moreover, BPS showed a higher potency to induce visceral fat formation in mice than BPA at the exposure concentration of 5 mg/kg/day for 14 days. Nontargeted metabolomic analysis showed that BPS exposure induced multiple metabolic disorders in the livers of BPS-treated mice. Interestingly, the body weight and volumes of visceral fat returned to normal by co-treatment with succinate in BPS-treated mice, suggesting that succinate might be the key molecule for the link between BPS and visceral obesity. Our findings highlight that BPS may act as an environmental obesogen to promote obesity.
研究领域:
环境科学
·
代谢生物学
·
毒理学
✓ 已验证
产品关联
DOI
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2023
·
IF 6.8
Wenjuan Zhang,Xuerui Ma,Yijia Zhang,Wanjing Tong,Xing Zhang,Yong Liang,Maoyong Song
Bisphenol P (BPP), structurally similar to bisphenol A, is commonly identified in the samples of environment, food, and humans. Unfortunately, very little information is currently available on adverse effects of BPP. The obesogenic effects and underlying mechanisms of BPP on mice were investigated in this study. Compared with the control, high-resolution microcomputed tomography (micro-CT) scans displayed that the visceral fat volume of mice was significantly increased at a dose of 5 mg/kg/day after BPP exposure for 14 days, whereas the subcutaneous fat volume remained unchanged. Nontargeted metabolomic analysis revealed that BPP significantly perturbed the metabolic pathways of mouse livers, and acetyl-CoA was identified as the potential key metabolite responsible for the visceral fat induced by BPP. These findings recommend that a great deal of attention should be paid to the obesogenic properties of BPP as a result of its widely utilized and persistence in the environment.
研究领域:
环境科学
·
代谢生物学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACTA PHARMACOLOGICA SINICA
·
2025
·
IF 8.4
Wang Hao,Zhang Li,Yang Wan-yue,Ji Xiao-yi,Gao An-qi,Wei Yi-hong,Ding Xin,Kang Yue,Ding Jian-hua,Fan Yi,Lu Ming,Hu Gang
Obesity increases the risk of depression. Evidence shows that peripheral inflammation, glycemic dysregulation, and hyperactivity within the hypothalamic-pituitary-adrenal axis are implicated in both obesity and depression. In this study we investigated the impact of visceral adipose tissue (VAT), a crucial characteristic of obesity, on stress susceptibility in obese mice. Age-matched mice were fed with chow diet (CD) or high-fat diet (HFD), respectively, for 12 weeks. CD mice were deprived of VAT and received transplantation of VAT from HFD mice (TransHFD) or CD mice (TransCD). Extracellular vesicles (EVs) were prepared from VAT of CD or HFD mice, and intravenously injected (100 μg, 4 times in 2 weeks) in naïve mice or injected into hippocampus (5 μg, 4 times in 2 weeks) through implanted bilateral cannula. Depression-like behaviors were assessed 14 days after transplantation. We showed that HFD mice exhibited significantly higher body weight gain and impaired insulin and glucose tolerance, accompanied by increased stress susceptibility. Transplantation of VAT or VAT-derived EVs from HFD mice caused synaptic damage and promoted stress susceptibility in recipient mice. Through inhibiting miRNA biogenesis in the VAT and miRNA sequencing analysis, we demonstrated that miR-140-5p was significantly upregulated in both VAT-EVs and hippocampus of HFD mice. Overexpression of hippocampal miR-140-5p in naïve mice not only facilitated acute stress-induced depression-like behaviors, but also decreased hippocampal CREB-BDNF signaling cascade and synaptic plasticity. Conversely, knockdown of miR-140-5p in the VAT, VAT-EVs or hippocampus of HFD mice protected against acute stress, reducing stress susceptibility that were mediated via CREB-BDNF pathway. In summary, VAT-EVs or the cargo miRNAs in obese mice promote synaptic damage and stress susceptibility, providing potential therapeutic targets for metabolism-related affective disorders.
研究领域:
医学
·
神经科学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2020
·
IF 5.977
Yan Liu,Lianying Zhang,Qinge Li,Han Dai,Ting Xiang,Gangyi Yang,Ling Li
In this work, a colorimetric assay for visfatin detection is described. The mixed valence state Ce-MOF (MVCM) modified by platinum nanoparticles (Pt NPs) is used as a novel catalyst. MVCM exhibits excellent intrinsic peroxidase-like catalytic activity due to the spontaneous recycling of the Ce(III)/Ce(IV) system. Pt NPs serve not only as a carrier of the –NH 2 -modified single strand DNA (S1) but also as a synergistic catalyst of MVCM. The capture probe (S2) attached to the streptavidin-modified magnetic beads (Mag-SA) could combine with the aptamer to form the Mag-SA/S2/aptamer complex. When in the presence of the target visfatin, aptamer specifically combines with the visfatin, which induces the release of Mag-SA/S2 from the Mag-SA/S2/aptamer complex. At this time, the [email protected] /S1 complex connects with the released Mag-SA/S2, which quickly catalyzes the 3,3,3′,3′-tetramethylbenzidine (TMB), leading to a color change. Under optimal conditions, the absorbance increases linearly when the concentration ranges from 1 ng mL −1 to 100 ng mL −1 , and the detection limit is as low as 0.11 ng mL −1 . Furthermore, Mag-SA/S2 can be reused at least five times by using the uracil-DNA glycosylase (UDG) and an external magnetic field. The proposed method shows satisfying reproducibility, stability, specificity, and sensitivity, and it was successfully applied to detect visfatin in spiked human serum samples. Thus, it has great potential for clinical research, detection, and catalytic applications.
研究领域:
分析化学
·
纳米技术
·
生物传感器
·
临床诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Surfaces and Interfaces
·
2023
·
IF 6.2
Hongyuan Cui,Lin Yang,Xueqi Fu,Guodong Li,Shu Xing,Xiao-Feng Wang
The simultaneous detection of multiple diabetes markers is important for the diagnosis and typing of diabetes because of the complex causes of diabetes. Two-dimensional titanium carbide (MXene), an emerging material, has great potential for biomedical sensing, in addition to its electrical applications. In this study, a fluorescence resonance energy transfer (FRET) aptasensor with high sensitivity and specificity was constructed using monolayer Ti 3 C 2 MXene for the simultaneous detection of insulin and visceral adipose tissue-derived serotonin (vaspin). Fluorescein-labeled insulin binding aptamers (IBAs) and Cy7-labeled vaspin-binding aptamers (VBAs) were attached to Ti 3 C 2 , and their fluorescence was effectively quenched by FRET between the fluorescein and Ti 3 C 2 . Insulin and vaspin were preferentially bound to IBA and VBA for a higher affinity. This process led to the subsequent shedding of fluorescein from Ti 3 C 2 , resulting in fluorescence recovery. The high fluorescence quenching efficiency and broad wavelength absorption of Ti 3 C 2 MXene enabled the quenching of two different, wide spectrum fluorescence at two different wavelengths, thus enabling the simultaneous detection of both insulin and vaspin. The aptasensor was highly sensitive, with a low detection limit of 36 pM for insulin and 45 pM for vaspin. Importantly, this Ti 3 C 2 -based aptasensor can precisely detect insulin and vaspin in human serum and diagnose a specific type of diabetes and identify its cause to facilitate subsequent treatment. The above results indicate that the Ti 3 C 2 -based aptasensor has promising applications in the clinical diagnosis and typing of diabetes mellitus.
研究领域:
生物医学工程
·
分析化学
·
糖尿病研究
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）