JOURNAL OF FOOD PROTECTION
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2020
·
IF 1.581
Hongying Li,Qiujin Zhu,Rui Chang,Ke Hu,Xuling Zhu,Aqi Xu,Shitao Xu,Pengyu Tang
ABSTRACT In order to reduce the health risks associated with red meat as listed by the World Health Organization, the work presented in this article aimed to elucidate the interaction between 5′-CMP–supplemented feed and N -glycolylneuraminic acid (Neu5Gc) in experimental animals in vivo. 5′-CMP was added to the diet of 90-, 180-, and 270-day-old Xiang pigs, and after 30 days, the Neu5Gc contents, physicochemical parameters, and free amino acid contents of muscle and internal viscera were measured by high-performance liquid chromatography coupled with fluorescence detection. The mechanism by which 5′-CMP affects Neu5Gc contents was investigated using molecular docking. Results show that 5′-CMP significantly decreased the Neu5Gc content in 180-day-old Xiang pigs ( P < 0.05) but had no effect on the Neu5Gc contents in 90- and 270-day-old Xiang pigs. Umami amino acids were significantly increased in 180-day-old Xiang pigs. In the 90- and 270-day-old pigs, histidine increased by 10.38 and 17.87%, respectively. The other free amino acids were either reduced or not affected. Moreover, the 5′-CMP–supplemented diet did not affect the physicochemical parameters of the longissimus muscle in the Xiang pigs. 5′-CMP could occupy almost all the sialyltransferase active-site residues but not His302 and showed inhibition of the sialyltransferase activity. The results provided an experimental basis for the subsequent reduction of Neu5Gc in red meat before slaughter.
研究领域:
营养学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
Environmental Science & Technology Letters
·
2021
·
IF 7.653
Wenjuan Zhang,Yue Gao,Cheng Chen,Aijing Li,Xuerui Ma,Yong Liang,Xinglei Yao,Maoyong Song,Guibin Jiang
Bisphenol S (BPS) is a bisphenol A (BPA) replacement that is widely present in the environment and in humans. The association between obesity and BPS has been reported in epidemiological studies; however, the obesogenic effect and related mechanism of BPS remain poorly understood. In this study, high-resolution microcomputed tomography analysis showed that the volume of visceral fat was significantly increased in BPS-treated mice. Moreover, BPS showed a higher potency to induce visceral fat formation in mice than BPA at the exposure concentration of 5 mg/kg/day for 14 days. Nontargeted metabolomic analysis showed that BPS exposure induced multiple metabolic disorders in the livers of BPS-treated mice. Interestingly, the body weight and volumes of visceral fat returned to normal by co-treatment with succinate in BPS-treated mice, suggesting that succinate might be the key molecule for the link between BPS and visceral obesity. Our findings highlight that BPS may act as an environmental obesogen to promote obesity.
研究领域:
环境科学
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代谢生物学
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毒理学
✓ 已验证
产品关联
DOI
高影响因子
Food Chemistry-X
·
2024
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IF 6.5
Sirong Huang,Xintong Yao,Boya Cao,Na Zhang,Olugbenga P. Soladoye,Yuhao Zhang,Yu Fu
The purpose of the present work was to encapsulate zingerone (a bioactive compound from ginger) by self-assembling peptides derived from fish viscera. The encapsulation conditions were investigated and the structure of fish peptides-zingerone complex was characterized. The interaction between zingerone and fish peptides was investigated using fluorescence spectroscopy. Further research was performed on the in vitro release of zingerone and fish peptide-zingerone as well as their antiproliferative effects on colon epithelial Caco-2 cells. The results demonstrated that zingerone can be successfully encapsulated by self-assembling peptides derived from fish viscera with high encapsulation efficiency and loading capacity. Furthermore, transmission electron microscope and confocal laser scanning microscope observations revealed the successful encapsulation of zingerone by fish viscera peptides. In addition, in vitro release and antiproliferative activity against Caco-2 cells can be significantly increased by encapsulating zingerone via peptide self-assembly. The current study advances knowledge of encapsulation of bioactive compounds through peptide self-assembly.
研究领域:
食品科学
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生物化学
·
医学
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产品关联
DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2023
·
IF 6.8
Wenjuan Zhang,Xuerui Ma,Yijia Zhang,Wanjing Tong,Xing Zhang,Yong Liang,Maoyong Song
Bisphenol P (BPP), structurally similar to bisphenol A, is commonly identified in the samples of environment, food, and humans. Unfortunately, very little information is currently available on adverse effects of BPP. The obesogenic effects and underlying mechanisms of BPP on mice were investigated in this study. Compared with the control, high-resolution microcomputed tomography (micro-CT) scans displayed that the visceral fat volume of mice was significantly increased at a dose of 5 mg/kg/day after BPP exposure for 14 days, whereas the subcutaneous fat volume remained unchanged. Nontargeted metabolomic analysis revealed that BPP significantly perturbed the metabolic pathways of mouse livers, and acetyl-CoA was identified as the potential key metabolite responsible for the visceral fat induced by BPP. These findings recommend that a great deal of attention should be paid to the obesogenic properties of BPP as a result of its widely utilized and persistence in the environment.
研究领域:
环境科学
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代谢生物学
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毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD SCIENCE
·
2020
·Vol. 85
·期 12
·4260-4270
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IF 2.479
Shu Guo,Jiao Wang,Chuanbo He,Haocheng Wei,Ying Ma,Hejian Xiong
Abalone viscera were byproducts of the abalone processing and usually discarded as wastes. In this study, we tried to obtain functional polysaccharides from abalone viscera by a combination of enzymatic hydrolysis, membrane separation, anion exchange chromatography, and gel filtration techniques. Abalone viscera underwent successive hydrolyzation with alcalase and flavourzyme. Each enzymolysis was followed by deproteinization via membrane separation. The final yield of crude abalone viscera polysaccharide (CAVP) was 19.72%; the polysaccharide content of CAVP was 51.75%. Furthermore, three fractions of polysaccharides (AVP1, AVP2, and AVP3) were isolated from the CAVP by anion exchange chromatography and gel filtration. The molecular weights of each AVP were 14.99 kDa, 58.48 kDa, and 39.63 kDa, with a carbohydrate content of 62.75, 23.09, and 44.67%, respectively. These AVPs showed excellent antioxidant activities in vitro. Our results provide a scientific basis for the further utilization of polysaccharides from abalone viscera. Practical Application This study demonstrated an eco-friendly approach for industrial production of high purity animal-derived polysaccharides without any environmental pollution caused by the viscera waste of abalone and promoting the comprehensive utilization of abalone resources.
研究领域:
食品科学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
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2019
·
IF 3.579
Jun Chen,Wenqi Cao,Peixiao Wei,Ting Li,Wuyin Weng
Speciation transformation of arsenic in the abalone viscera hydrolysate fraction (AVHF) was evaluated using in vitro and in vivo methods to determine its safety given that AVHF is rich in arsenic. The dimethylarsinic acid (DMA) proportion and some free amino acid contents increased, whereas arsenobetaine (AB) proportion decreased when AVHF was digested by pepsin. However, molecular weight distribution was unchanged, and no obvious changes were found in the intestinal medium. In the single-dose experiment, the AB concentration on the mouse plasma rapidly increased, which reached up to 12.53 ng/mL in 2 h after the administration of AVHF (10 g/kg body weight) and reduced to half of the maximum at 8 h after administration. Furthermore, alanine (Ala) content in the urine of mice increased at 8 h after AVHF administration, suggesting that Ala might be chelated with arsenic and could not be absorbed well. Long-term experiments showed that AB was not accumulated in mice tissue/organ. However, some AB could be converted into DMA, which was mainly accumulated in mice hair. The in vivo experiments also suggested that the AVHF is safe as health food.
研究领域:
营养学
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毒理学
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食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2025
·Vol. 26
·期 7
·3273
·
IF 4.9
Tianxu Liu,Muyuan Ma,Yonglin Wu,Ismail Muhammad Asif,Daosen Chen,Lichong Liu,Minghui Zhang,Yijie Chen,Bin Li,Ling Wang
As the major polysaccharide in brown algae, fucoidan possesses broad biological abilities and has been reported to improve gastrointestinal health. Functional dyspepsia, a common non-organic disease, is a complex of symptoms mainly characterized by pathogenesis, such as visceral hypersensitivity, gastric dysmotility, and inflammation. To date, the effects of fucoidan in regulating functional dyspepsia with visceral sensitivity remains unclear. In the current study, iodoacetamide was employed to establish a mouse model of visceral hypersensitivity. Meanwhile, fucoidan was orally administrated for fourteen days. Indicators were conducted to evaluate the potential of fucoidan as the ingredient of complementary and alternative medicine for functional dyspepsia, such as levels of serum hormones, expression of receptors, and gut microbial profile. The results show that oral administration of fucoidan led to significant reductions in the secretion of 5-hydroxytryptamine, cortisol, and corticosterone. Additionally, it decreased the expression of 5-hydroxytryptamine-3receptors, with regulation of 5-hydroxytryptamine metabolism and improvement of gut microbial imbalance. The above results suggest fucoidan could ameliorate visceral hypersensitivity by modulating 5-HT metabolism and microbiota. The current findings indicate that fucoidan has potential as a biological component in the adjuvant treatment of functional dyspepsia and for its expanded utilization in the food and medical fields.
研究领域:
胃肠病学
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营养学
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药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2021
·
IF 7.514
Chen He,Jun Cao,Yuyan Bao,Zexin Sun,Zhongyuan Liu,Chuan Li
A lipidomic evaluation was performed on the tilapia muscle, head and viscera, including studying the composition, distribution and stereospecific characteristics of fatty acids and lipid species. The head and viscera lipids were significantly richer than the muscle lipids. Triacylglycerols were the predominant fraction (over 80% of total lipid in the muscle and head). Additionally, polyunsaturated fatty acids had higher percentages in phospholipids (30.35–52.05% of total fatty acids) than in triacylglycerols (18.11–25.15%). The C52:2 and C52:3 were the most abundant triacylglycerols, which indicates the potential application in infant food. Moreover, 622, 530 and 513 lipids were identified using ultraperformance liquid chromatography-quadrupole time-of-flight-mass spectrometry in the muscle, head and viscera, respectively. The three tilapia parts were distinguished using multivariate analysis. Five fatty acids and 33 lipid species were considered as the potential biomarkers. This comprehensive analysis will help to evaluate the lipid nutritional values and facilitate exploitation in tilapia consumption and processing.
研究领域:
营养学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PeerJ
·
2018
·
IF 2.118
Xueqin Wang,Huahua Yu,Ronge Xing,Xiaolin Chen,Song Liu,Pengcheng Li
Mackerel ( Pneumatophorus japonicus ) viscera contain large amount of protein. We used five proteases to hydrolyze the viscera, and the hydrolysate treated by neutrase exhibited the highest nitrogen recovery (NR). Then we optimized the preparation conditions for mackerel viscera hydrolysate (MVH) by response surface methodology and investigated the antioxidant activity of MVH. The optimal conditions were as follows: enzyme concentration of 1,762.87 U/g, pH of 6.76, temperature of 43.75 °C, extraction time of 6.0 h and water/material ratio of 20.37 (v/w), and the maximum NR was 37.84%. Furthermore, the molecular weight distribution of MVH was almost below 3,000 Da determined by TSK G2000 SWXL gel filtration chromatography, and the MVH exhibited good antioxidant activities in various in vitro assays, including DPPH radical, hydroxyl radical and superoxide anion scavenging activities, reducing power and similar effectivelness as butylated hydroxytoluene and Vitamin E to inhibit lipid peroxidation. The results suggested that MVH could be used as a potential source of antioxidant peptide in food industries.
研究领域:
食品科学
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生物化学
✓ 已验证
DOI
PubMed
高影响因子
Journal of Aquatic Food Product Technology
·
2019
·
IF 0.707
Wuyin Weng,Jieyu Li,Ting Li,Yanjun Ye
A fraction with molecular weight of 0.2–1.0 kDa was prepared from abalone viscera hydrolysate by ultrafiltration and nanofiltration. The contents of protein, carbohydrate, and fat in abalone viscera were 45.75%, 18.19%, and 13.60%, respectively. The protein in abalone viscera hydrolysate faction (AVHF) reached up to 75.73%, while carbohydrate and fat fell to 1.80% and 0.20%, respectively. The leucine (Leu) content was the highest in the AVHF. On the other hand, the hydroxyl and 2,2-diphenyl-1-picrylhydrazyl radical scavenging activities and reducing power increased with increased concentration of AVHF. The inhibiting ability of AVHF on lipid peroxidation was higher than that of ascorbic acid. The total content of K, Na, and P in AVHF was 9.78 g/100 g, and total arsenic content reached 16.23 mg/kg. The proportion of arsenobetaine and arsenocholine accounted for more than 96%, while the proportion of arsenate was 0.47%, and arsenite was not detectable. It is concluded that the AVHF from abalone viscera is a safe natural antioxidant.
研究领域:
食品科学
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营养学
·
毒理学
✓ 已验证
产品关联
DOI
高影响因子
PROCESS BIOCHEMISTRY
·
2016
·
IF 2.529
Ru Song,Kai-qiang Zhang,Rong-bian Wei
Squid viscera, one of the major by-products in squid processing, were hydrolyzed to generate bioactive autolysates using their endogenous proteases. In vitro antioxidative activities of squid viscera autolysates (SVAs) were evaluated. The SVAs demonstrated strong activity on scavenging 1,1-diphenyl-2-picryl-hydrazil (DPPH) and hydroxyl radicals, as well as reducing power ability. The SVAs were purified using size exclusion chromatography and RP-HPLC. Nineteen peptides were identified in the active fraction SVAs3 by liquid chromatography-electrospray ionization/multi-stage mass spectrometry (LC-ESI-Q-TOF-MS/MS). Among these identified peptides, six peptides with relatively small molecular weights (700–800 Da), LLAPPER, FPGLADR, WVAPLK, FFNPVH, FNVVLK and LELPLK, were synthesized for assaying antioxidative activity in vitro. Peptide WVAPLK demonstrated strong scavenging effects on free radicals, with the IC 50 values of 0.82 ± 0.08 mg/mL (or 1.14 ± 0.11 mM) for DPPH radical and 1.85 ± 0.04 mg/mL (or 2.60 ± 0.06 mM) for hydroxyl radical, respectively. The low molecular weight and hydrophobic residue W at the N-terminus and basic residue K at the C-terminus as well as specific residues P and L within sequence should play a key role for the high antioxidative activity of WVAPLK. The results suggested that the SVAs could be used as a source of antioxidants and peptides.
研究领域:
食品科学
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生物化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF SUPERCRITICAL FLUIDS
·
2019
·
IF 3.481
Gengxin Hao,Wenqi Cao,Ting Li,Jun Chen,Jinli Zhang,Wuyin Weng,Kazufumi Osako,Huifeng Ren
The effect of temperature on the main components and antioxidant activities of abalone viscera subcritical water extract (AVSWE) was investigated. With the increase in temperature from 110 °C to 230 °C, the protein content in AVSWE increased from 57.4% to 68.5%, while the carbohydrate content decreased from 17.9% to 0.8%. The highest percentage of 1000–5000 Da peptide fraction and total essential amino acid content was found in the AVSWE extracted at 170 °C. The transformation rate of arsenobetaine into dimethylarsinic acid in AVSWE increased dramatically with the increase subcritical water temperature from 170 °C to 200 °C. The results of DPPH and hydroxyl radical scavenging activity, reducing power and lipid peroxidation inhibition effect showed that AVSWE with the highest antioxidant activities was extracted at 170 °C. However, the subcritical water temperature for AVSWE extraction should not exceed 140 °C according to arsenic speciation.
研究领域:
食品科学
·
生物化学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）