LANGMUIR
·
2015
·
IF 4.457
Jun Yang,Zan Hua,Tao Wang,Bo Wu,Guangming Liu,Guangzhao Zhang
Protein adsorption is an important issue in biorelated fields. We have investigated the protein adsorption on the poly(ionic liquid) (PIL) brushes in the presence of different types of counterions. The protein adsorption is driven by a decrease in osmotic pressure within the brushes with an increase in entropy via the release of counterions. Our study demonstrates that counterion specificity has a significant influence on protein adsorption on the PIL brushes. There have been two different regimes for counterion-specific protein adsorption. When the released counterions cannot bind to the protein surface, the counterion-specific protein adsorption is dominated by the ion-specific counterion condensation within the PIL brushes. If the released counterions can bind to the protein surface, then counterion-specific protein adsorption is dominated by the ion-specific rebinding of released counterions on the protein surface. This work opens up a new opportunity for controlling protein adsorption on polyelectrolyte brushes.
研究领域:
生物物理学
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材料科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2020
·
IF 10.652
Yilin Wu,Wendong Xing,Faguang Ma,Jia Gao,Xinyu Lin,Jian Lu,Chao Yu,Ming Yan
Three-dimensional (3D) basswood materials were successfully applied to construct molecularly imprinted nanocomposite membranes for the first time. Importantly in this work, the TiO 2 @PDA-based modification layers were initially achieved on basswood surfaces. And 3D mesoporous wood-based molecularly imprinted membranes (3DW-MIMs) with sandwich-like TC-imprinted layers were finally synthesize through a re-modified surface-initiated sol-gel technique. Interestingly, based on the TiO 2 -PDA@basswood surfaces, great increasement of TC-imprinted sites could be prepared during the sol-gel imprinting process. So it was no surprise that excellent rebinding capacity (150.5 mg g −1 ), fast adsorption kinetics and high permselectivity coefficients (more than 9.0) of 3DW-MIMs could be successfully achieved. Furthermore, an important phenomenon had also been found, i.e. the PDA-modified layers caused significant promotions to the rebinding capacities of the as-prepared 3DW-MIMs, that is to say, more and more TC-imprinted sites could be synthesized because of the PDA-modified surfaces. In addition, the as-prepared 3DW-MIMs were synthesized without complicated procedures and polluting the environment. Finally, the experimental results mentioned above, together with the green synthesis processes, strongly demonstrated that our synthesis methodology of 3DW-MIMs had great potential for applications in various fields of selective separation, chemical industry, environment, biological medicine and so on.
研究领域:
材料科学
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环境科学
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化学工程
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生物医学工程
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产品关联
DOI
高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2016
·Vol. 52
·期 8
·4713-4726
·
IF 2.302
Zhang Xiaoyan,Hu Xiaoling,Guan Ping,Zhang Nan,Li Ji,Du Chunbao
In this work, super-paramagnetic interface molecularly imprinted microspheres (MIMIMs) were prepared using an immunostimulating hexapeptide as template via photo-initiated Pickering emulsion polymerization by the self-assembly of the template-immobilized silica nanoparticles at the oil–water interface under room temperature. The whole experiment process was tracked and analyzed by various characterization methods. Results manifest that the obtained MIMIMs showed excellent recognition property and rapid-efficiency separation for template peptide due to their accessible imprinted sites and desired magnetic susceptibility. Moreover, MIMIMs were used in competitive rebinding and reusability tests, which demonstrated their potential application in the enrichment of IHH and sustainable performance in resource-saving.
研究领域:
材料科学
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纳米技术
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生物医学工程
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分析化学
✓ 已验证
产品关联
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2022
·
IF 9.136
Yun Cheng,Xiaobin Zhao,Qiong Zhang,Xueqin Li,Zhong Wei
Acteoside (ACT) is an active component of Cistanche tubulosa with rare content and complex structure. The development of ACT-based molecularly imprinted hybrid membranes (A-MIHMs) with high rebinding capabilities and selectivity remains a challenge to obtain high purity of ACT. Here a series of A-MIHMs was designed for highly selective separation of ACT. The A-MIHMs were fabricated by incorporating imprinted nanofillers made of ACT-imprinted surface molecularly imprinted polymers and polyethylenimine modified mesoporous silica (SMIP-PEI/MCM-41) into polyvinylidene fluoride (PVDF) powders. The as-prepared optimum A-MIHMs-400 exhibited the highest rebinding capabilities of 96.24 mg/g, an excellent rebinding selectivity of 4.48, and permselectivity of 6.37 for the ACT. The following reasons were attributed to the results: first, the incorporated SMIP-PEI/MCM-41 nanofillers were distributed homogeneously into the A-MIHMs, and the pores of the membrane were tailored to form the interconnected imprinted reticular structure, and it can capture abundant ACT, resulting in highly selective separation of ACT. Second, the formed imprinted cavities and sites on the imprinted reticular structure can uniquely recognize ACT, enhancing A-MIHMs selectivity for ACT. Third, the amino groups of PEI acted as affinity sites with a high-affinity for the hydrophilic molecule of ACT, allowing A-MIHMs to bind the ACT with high rebinding capabilities and selectivity. After eight cycles, the regeneration rate approached 90%, indicating that A-MIHMs were highly renewable. The design of MIHMs with an imprinted reticular structure would provide a new strategy for the highly selective separation of active components from natural products.
研究领域:
材料科学
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化学工程
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药学
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分离技术
✓ 已验证
产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
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2013
·
IF 5.437
Huachang Chen,Juan Kong,Dongying Yuan,Guoqi Fu
Molecularly imprinted polymers against proteins are regarded as promising substitutes for natural antibodies, but have been frustrated with the problems including reduced interaction between functional monomers and protein template in the aqueous media required during their synthesis and restricted mass transfer across the resulting crosslinked polymer matrixes . For addressing these issues, herein we proposed a strategy for imprinting of a protein on the surface of nanoparticles using a metal chelating monomer. With lysozyme as a model protein template and Cu 2+ chelating N-(4-vinyl)-benzyl iminodiacetic acid as the coordination monomer along with other monomers, protein imprinted polymer nanoshells were formed over vinyl-modified silica nanoparticles via surface polymerization in high-dilution monomer solution. The feed concentration of the crosslinking monomer was optimized toward achieving the best imprinting effect. Compared with the related imprinted materials reported previously, the resultant core–shell imprinted particles showed greatly faster binding kinetics , elevated rebinding capacity and selectivity . More importantly, noticeably high binding affinity was achieved with an estimated dissociation constant of 4.1×10 −8 M which is comparable to that of conventional antibodies
研究领域:
材料科学
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聚合物化学
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蛋白质工程
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of the Taiwan Institute of Chemical Engineers
·
2018
·
IF 3.849
Jia Gao,Yilin Wu,Jiuyun Cui,Xiuling Wu,Minjia Meng,Chunxiang Li,Li Yan,Shi Zhou,Lili Yang,Yongsheng Yan
Inspired from the bioadhesive surface modified technology of polydopamine (pDA) and the radical polymerization method of amphiphilic grafted copolymer (ACMO@PVDF), the bioinspired porous enoxacin-imprinted nanocomposite membranes (EINCMs) were synthesized by developing the multi-walled carbon nanotubes (MCNTs)-based nanocomposite structure. The EINCMs were prepared through immersion phase inversion method and sol–gel blot imprinted method for selective purification and separation of enoxacin. Testing results indicated that the surface physicochemical properties such as higher micropores, significantly improved hydrophilicity and enhanced antifouling ability, could be successfully obtained. Results showed that the specific adsorption capacity was markedly enhanced from 3.72 mg g −1 , which was for the non-imprinted nanocomposite membrane (NINCMs), to 31.56 mg g −1 for the imprinted one. In addition, the imprinting factor β was proved as high as 4.44 and the perm-selectivity of membrane was also measured in the form of separation factor γ , which was calculated as 6.03. Furthermore, the rebinding capacities reached about over 90. 36% of the initial adsorption capacity after 5 cycles of adsorption/desorption. The rebinding capacity just only declined to 82.89% after another 5 cycles rebinding circulations. The highly stable stability and homogeneous growth of enoxacin-imprinted layers on pDA@MCNTs surfaces might probably be the reason for desirable performance and stability. The as-obtained results could be concluded that this synthesis method would provide wastewater treatments containing enoxacin with a highly stable and selective separation performance.
研究领域:
材料科学
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环境工程
·
纳米技术
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膜技术
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）