FOOD CHEMISTRY
·
2024
·
IF 8.5
Yali Wang,Shuangbo Zhang,Jiang Bian,Joseph Brake,Chaoyang Luo,Chuanwen Sun,Xiaobin Wu
Mercury has caused severe harm to the environment and human health. A novel biological screen method was developed and identified a Hg 2+ chelator BDTH2. Both biological and chemical methods demonstrated BDTH2 displayed a high specificity and strong binding capacity for Hg 2+ . Toxicological experiments of BDTH2 demonstrated its good safety. To enhance the sensitivity, functional composite BDTH2-AuNPs were synthesized, and the system exhibited strong specificity to Hg 2+ and excellent anti-interference capabilities. The optimized colorimetric method and the paper test strip method showed a visual detection limit of 3 nM less than 30 s and 5 nM within 1 min respectively. This system was evaluated for detecting spiked Hg 2+ concentrations in environmental water and six agricultural food samples and showed high consistence with those of ICP-MS. Therefore, this study provides a novel, rapid, and economical method for on-site detecting of Hg 2+ in water and agricultural products.
研究领域:
环境科学
·
分析化学
·
毒理学
✓ 已验证
DOI
高影响因子
MOLECULES
·
2022
·Vol. 27
·期 13
·4294
·
IF 4.927
Hui Li,Fei Li,Fang Liu,Xiao Chen,Wenyuan Xu,Liang Shen,Jingkun Xu,Rui Yang,Ge Zhang
In view of that conjugated polymers (CPs) are an attractive option for constructing high-sensitive Cr2O72−sensors but suffer from lacking a general design strategy, we first proposed a rational structure design of CPs to tailor their sensing properties while validating the structure-to-performance correlation. Short side chains decorated with N and O atoms as recognition groups were instructed into fluorene to obtain monomers Fmoc-Ala-OH and Fmoc-Thr-OH. Additionally, their polymersP(Fmoc-Ala-OH)andP(Fmoc-Thr-OH)were obtained through electrochemical polymerization.P(Fmoc-Ala-OH)andP(Fmoc-Thr-OH)with high polymerization degrees have an excellent selectivity towards Cr2O72−in comparison to other cations and anions. Additionally, their limit of detection could achieve 1.98 fM and 3.72 fM, respectively. Especially, they could realize the trace detection of Cr2O72−in agricultural products (red bean, black bean, and millet). All these results indicate that short side chains decorated with N and O atoms functionalizing polyfluorene enables the ultra-trace detection of Cr2O72−. Additionally, the design strategy will spark new ideas for the construction of highly selective and sensitive Cr2O72−sensors.Keywords:Cr2O72−;design strategy;conjugated polymer;ultra-trace detection;agricultural products
研究领域:
化学传感器
·
材料科学
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产品关联
DOI
PubMed
高影响因子
Toxins
·
2018
·Vol. 11
·期 1
·6
·
IF 3.273
Ming Li,Mingna Sun,Xia Hong,Jinsheng Duan,Daolin Du
A total of 328 agricultural product samples highly suspected to be contaminated, from flour companies, feed companies, and livestock farms throughout China, were surveyed for deoxynivalenol (DON) contamination using a self-assembly enzyme-linked immunosorbent assay (ELISA) kit. An ELISA kit for DON was developed with a 4.9 ng mL−1limit of detection (LOD) in working buffer and a 200 ng g−1LOD in authentic samples. The DON contamination detection rate was 88.7%, concentrations ranged from 200.9 to 6480.6 ng g−1, and the highest DON contamination was found in distillers’ dried grains with solubles with an average of 3204.5 ng g−1. Wheat bran and wheat were found to be the most commonly contaminated samples, and the corn meal samples had the lowest average DON level. This ELISA kit is a powerful alternative method for the rapid, sensitive, specific, accurate, and high-throughput determination of DON and can meet the maximum requirement levels. This survey suggests that DON contamination in the Chinese market is serious, and the contamination risk deserves attention. Essential preventive measures should be implemented to ensure food safety and human health.Keywords:deoxynivalenol;contamination;survey;ELISA kit;agricultural productKey Contribution:A total of 328 agricultural product samples highly suspected to be contaminated were surveyed for deoxynivalenol using a self-assembly ELISA kit.
研究领域:
食品安全
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Science
·
2024
·
IF 14.3
Zichen Zheng,Kewei Liu,Yiwen Zhou,Kaichun Xu,Yifan Luo,Jiabao Ding,Carla Bittencourt,Marc Debliquy,Chao Zhang
La-decorated Bi 2 O 2 CO 3 (BCO-La) microspheres are synthesized using a facile wet chemical strategy for sensing low-concentration nonanal (C 9 H 18 O) at room temperature. These BCO-La gas sensors are applied to evaluate agricultural product quality, specifically for cooked rice. The sensitivity of the BCO-6La sensor significantly surpassed that of the pure BCO sensor, achieving a response value of 174.6 when detecting 30 ppm nonanal gas. Notably, the BCO-6La sensor demonstrated a faster response time (36 s) when exposed to 18 ppm of nonanal. Additionally, the selectivity toward nonanal gas detection is higher (approximately 4–24 times) compared to interfering gases (1-octanol, geranyl acetone, linalool, hexanal, 2-pentyfuran, and 1-octen-3-ol) during cooked rice quality detection. The gas sensing mechanism and the factors contributing to the enhanced sensing performance of the BCO-La microspheres are demonstrated through in situ FT-IR spectra and DFT analysis while the realistic detection scenario is carried out. In a broader context, the reported sensors here represent a novel platform for the detection and monitoring of gases released by agricultural products during storage.
研究领域:
材料科学
·
传感器技术
·
农业科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL SCIENCES
·
2023
·Vol. 39
·期 7
·1151-1161
·
IF 1.6
Zhang Zhihui,Zhang Jingyu,Li Mufei,Jin Xiangzi,Yao Liping,Wang Wenyuan,Liu Jinsong,Li Zuguang
This work first proposed a novel green and efficient method based on Quick, Easy, Cheap, Efficient, Rugged, and Safe pretreatment (QuEChERS) combined with switchable hydrophilic solvent homogeneous liquid–liquid microextraction (SHS-HLLME) for trace determination of triazole fungicides (TFs) in agricultural products such as vegetables and fruits by gas chromatography–mass spectrometry (GC–MS). N , N -Dimethyl benzylamine was used for the synthesis of SHS. Box–Behnken design was applied for the optimization of extraction conditions and a mathematical model was obtained. Ultimately, 0.50 mL SHS, 1.0 mL 10 mol L −1 sodium hydroxide, and 45 s ultrasonic time were determined as optimal conditions for the SHS-HLLME method. The limit of detection and limit of quantification determined using the optimal method (SHS-HLLME/GC–MS) were 0.13–0.27 ng mL −1 and 0.43–0.90 ng mL −1 , respectively. In addition, the SHS-HLLME method under optimal conditions was combined with the traditional QuEChERS method to realize the advancement of the SHS-HLLME method from simple to complex matrix analysis, and the QuEChERS–SHS-HLLME method was successfully applied to the analysis of TFs in cucumbers, tomatoes, watermelon and grapes in agricultural products. Matrix-matched calibration standards were used to improve the accuracy of TFs in spiked cucumber samples to obtain recovery results close to 100%. It was shown that the new method is green and rapid, enabling fast and inexpensive sample pretreatment with up to 100-fold enrichment factor and low detection limit compared with the original QuEChERS method. Graphical abstract
研究领域:
分析化学
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环境科学
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食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Foods
·
2024
·Vol. 13
·期 10
·1499
·
IF 4.7
Xuan Hu,Li Liu,Maomin Peng,Dan Zheng,Hong Xia,Youxiang Zhou,Lijun Peng,Xitian Peng
A novel co-bonded octyl and pyridine silica (OPS) sorbent was prepared and applied for the solid phase extraction (SPE) of cyclopiazonic acid (CPA, a type of mycotoxin) in feed and agricultural products for the first time. A simple mixed-ligand one-pot reaction strategy was employed for OPS sorbent preparation. Nitrogen adsorption–desorption measurements, elemental analysis (EI), thermal gravimetric analysis (TGA), and Fourier transform infrared spectroscopy (FT-IR) analysis demonstrated the successful immobilization of octyl and quaternary ammonium groups onto the surface of silica gel. The large specific surface area, high-density functional groups, and mixed-mode anion-exchange characteristics of these silica particles made them the ideal material for the efficient extraction of CPA. Additionally, the OPS sorbents displayed excellent batch-to-batch reproducibility, satisfactory reusability, and low cost. The SPE parameters were optimized to explore the ionic and hydrophobic interactions between CPA and the functional groups, and the ultra-high performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry (UPLC-MS/MS) parameters were optimized to obtain a desirable extraction efficiency and high sensitivity to CPA. Meanwhile, the OPS sorbent presented a satisfactory extraction selectivity and low matrix effect. Under the optimized conditions, our developed CPA detection method was used to determine CPA level in rice, wheat flour, corn flour, peanut, and feed samples, exhibiting a lower detection limit, better linearity, higher sensitivity, and satisfactory extraction recovery rate than previously reported methods. Therefore, our method can be preferentially used as a method for the detection of CPA in agricultural products and feeds.
研究领域:
分析化学
·
食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Ge Zhang,Hui Zhang,Jie Zhang,Wanchuan Ding,Jingkun Xu,Yangping Wen
A dissolvable conjugated polymer oligo(1-pyreneboronic acid) (OPYBA) as the fluorescent sensor was facilely electrosynthesized for highly selective analysis of Cu 2+ at ultra-trace levels in real samples. OPYBA was obtained by potentiostatical electropolymerization of corresponding monomer at 1.0 V vs. Ag/AgCl in acetonitrile containing tetrabutylammonium tetrafluoroborate and dedoped with dilute HCl. The tetrahydrofuran-solvable OPYBA showed good fluorescent properties and could specifically recognize Cu 2+ , and then it was employed for the quantification of Cu 2+ in linear ranges of 25 pM–88.2 μM and 188 μM–750 μM with a low detection limit of 23 pM. As the fluorescent sensor, OPYBA displayed a high sensitivity and selectivity for Cu 2+ over other substances existing in environment and agro-products such as metal ions, anions, organic acids, amino acids, and carbohydrates. Also, it was used to detect Cu 2+ in tap water, lake water, white rice, Chinese cabbage, and cropland soil samples with satisfactory results, revealing that OPYBA could be as a practical sensing platform for ultra-trace analysis of Cu 2+ in environment and agro-product samples.
研究领域:
分析化学
·
环境科学
·
农业科学
✓ 已验证
DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2020
·
IF 6.551
Mei Du,Qian Yang,Weimei Liu,Yuan Ding,He Chen,Xiude Hua,Minghua Wang
Imidacloprid is the most widely used neonicotinoid insecticide and has been reported to pose a threat to ecological security and human health. Therefore, simple-to-operate and highly sensitive methods for the detection of trace levels of imidacloprid are necessary. Here, we isolated two phage-borne peptides that compete with imidacloprid to bind the monoclonal antibody (mAb) 3D11 from phage display peptide libraries. A phage-enzyme-linked immunosorbent assay (P-ELISA) and two phage time-resolved fluoroimmunoassays (P-TRFIAs) for the detection of imidacloprid were developed using the phage-borne peptides as substitutes for chemically synthesized antigens. After systematic optimization, the half-maximum inhibition concentrations (IC 50 ) of the P-ELISA, P-TRFIA-1, and P-TRFIA-2 were 0.067 ng mL −1 , 0.085 ng mL −1 , and 0.056 ng mL −1 , respectively. Based on their IC 50 values, the sensitivities of the P-ELISA and P-TRFIAs were more than four times greater than those of previous immunoassays. Additionally, the immunoassays showed satisfactory recovery in the detection of spiked samples and good correlation with high performance liquid chromatography (HPLC) for the detection of samples containing incurred residues.
研究领域:
环境科学
·
分析化学
·
农业科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Hexue Li,Cui Wang,Hui Shi
Cross-contamination is a major food safety risk during the harvesting and processing of fresh produce, leading to significant losses in global human well-being and the economy. The surface of food contact areas is a high-risk zone for cross-contamination. Therefore, developing an effective antimicrobial coating for food-contact surfaces is essential. This study developed a smart antimicrobial coating that self-regulated in response to environmental conditions, via grafting the stimuli-responsive polymer polyacrylic acid (PAA) and the phage-derived endolysin Lysin81 onto ZnO nanocolumns. During the initial stage of bacterial adhesion, the surface of the nanocolumns exhibited significant mechanical bactericidal activity, while the super hydrophilic PAA layer effectively inhibited bacterial adhesion. At a later stage, when numerous live and dead bacteria adhered to the surface of the nanocolumns, the PAA chains disintegrated, exposing the underlying layer of endolysin that lysed the compromised bacteria. In addition, as the environmental pH increases, the attached dead bacteria can be released once the PAA chains regain their hydrophilicity. This research aimed to apply the antibacterial coating to stainless steel surfaces used in food processing, potentially enhancing surface hygiene and preventing cross-contamination of fresh produce.
研究领域:
食品科学
·
生物材料
·
微生物学
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2022
·Vol. 12
·期 9
·5540-5545
·
IF 4.036
Chengnan Xu,Mei Lin,Chaonan Song,Danli Chen,Caimiao Bian
Based on the colorimetric analysis of gold nanoparticles and a smartphone readable strategy, a stable, sensitive, and visual method was established for rapid detection of acetamiprid residues in agricultural products. By optimizing the key parameters, the detection process only took 40 minutes with good specificity. The acetamiprid aptamer can help AuNPs to resist salt-induced aggregation. Conversely, in the presence of acetamiprid, the anti-protection is weakened and the AuNPs aggregated with the color change of the solution. The photographs of the solution are recorded by the smartphone and analyzed through image processing. In the range from 25 to 300 μM the method can realize a quantitative analysis of acetamiprid, and the detection limit is about 3.81 μM. Excellent recoveries are taken in samples of cucumber, cabbage, and river water, ranging from 96.78% to 129.95%. These results show no significant difference from the results obtained by the microplate reader. What's more, the method employs a smartphone to read without the assistance of professional equipment, which greatly reduces the cost of detection, and shows a promising application prospect for on-site rapid detection of acetamiprid.
研究领域:
分析化学
·
环境科学
·
农业科学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2025
·
IF 2.5
Huanjing Chen,Wanqiao Bai,Hongmei Chai,Loujun Gao
To achieve the rapid and sensitive detection of trace methyl parathion (MP) in river water and edible agricultural products, the catalytic composite material zirconium hydroxide (Zr(OH)4)/zeolite imidazolate framework-90 (ZIF-90) was prepared by a one-step synthesis method. The composite was further incorporated with a conductive poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) hydrogel to prepare a modified electrode. Then, an electrochemical sensing method based on the PEDOT:PSS/Zr(OH)4/ZIF-90 composite was successfully established for the analysis and detection of MP. The component ratio of the composite material, modification amount and pH value of the detection substrate were optimized. The electrochemical properties of the composite modified electrode were evaluated by cyclic voltammetry (CV) and differential pulse voltammetry (DPV) techniques, and the results confirmed that it had good electrochemical properties. The electrochemical response of the established sensing method to MP showed a good linear relationship in the concentration range of 1–250 µmol L−1, and its detection limit and sensitivity reached 0.74 µmol L−1 and 0.09 µA µmol−1 L−1, respectively. The interference of common coexisting ions on the detection performance of the PEDOT:PSS/Zr(OH)4/ZIF-90 modified electrode was further investigated, and it was applied for the quantitative analysis of MP in actual river water and apple samples. The electrochemical sensing method developed in this work provides a new way for the efficient detection of MP residues in environmental water and agricultural products.
✓ 已验证
产品关联
DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2022
·
IF 10.753
Xue Liu,Jiao Li,Yue Huang,Zhongrong Zhang,Qing Lin,Pengliang Xia,Fanyu Kong,Jun Qiu,Song Fang,Xiude Hua
Quizalofop-p-ethyl is a widely used herbicide that poses a threat to human health and environmental safety. In this study, anti-quizalofop-p-ethyl monoclonal antibodies (mAbs) were prepared and used to develop a gold nanoparticle-based lateral flow immunoassay (AuNP-LFIA) for the detection of quizalofop-p-ethyl in agriproducts and environmental samples. Four hybridoma cell lines were obtained, among which 5B6D10E11 secreted mAb with the highest sensitivity, with a 50 % inhibition concentration of 4.57 ng/mL in the indirect competitive enzyme-linked immunosorbent assay. After optimization, the AuNP-LFIA strip based on the mAb (5B6D10E11) showed a visual detection limit of 10 ng/mL, and the results could be directly determined by the naked eye within 8 min. The cross-reactivity of the AuNP-LFIA for analogs of quizalofop-p-ethyl was negligible except for quizalofop-p-acid. The established AuNP-LFIA was proven to be accurate and precise based on the recovery test. Furthermore, the detection results of AuNP-LFIA were consistent with those of ultra-high-performance liquid chromatography tandem mass spectrometry.
研究领域:
环境科学
·
分析化学
·
农业科学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）