PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
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2025
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IF 4
Linlin Liu,Min Shi,Yanyan Wu,Jiali Hao,Junxiu Guo,Shanshan Li,Pingli Dai,Jing Gao
The widespread use of pesticides poses a significant threat to honeybee health by impacting their survival, behavior, immune function, and detoxification capacity. While phytochemicals such as resveratrol (RSV) have shown potential in mitigating oxidative stress and enhancing antioxidant defenses, their role in improving honeybee tolerance to pesticide exposure remains underexplored. In this study, we investigated the effects of RSV supplementation on honeybees exposed to three pesticides: dinotefuran (DIN), tebuconazole (TEB), and deltamethrin (DEL). The results showed that RSV supplementation significantly improved survival, feed intake, mobility, and gustatory sensitivity, indicating its protective effects against pesticide toxicity. Furthermore, RSV helped normalize impaired detoxification enzyme activities, including SOD, POD, catalase, and glutathione reductase, and reduced ROS levels and lipid peroxidation. Gene expression analysis revealed that RSV modulates Toll pathway-related genes like defensin and apidaecin , alleviating immune suppression caused by pesticides. Additionally, RSV influenced the insulin/insulin-like growth factor signaling (IIS) pathway by reducing ilp1 and inr1 expression, potentially mitigating metabolic stress. These findings demonstrate that protective effects of RSV may be linked to its ability to counter oxidative stress, restore mitochondrial function, and enhance energy metabolism. Furthermore, RSV is widely available, cost-effective, and easily incorporated into bee feed, making it feasible for large-scale application. This study highlights the protective role of RSV in pesticide detoxification in honeybees, offering new perspectives for honeybee health management and environmental toxicology research. By reducing the adverse effects of pesticides on honeybees, the application of RSV not only contributes to maintaining ecological balance but also supports sustainable agricultural practices. Future research should focus on optimizing its dosage, evaluating long-term effects, and investigating its impact on colony dynamics to facilitate its practical implementation in apiculture.
研究领域:
昆虫学
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环境毒理学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
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2024
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IF 8.1
Liangjie Tang,Shuiqing Chen,Ning Wang,Xiaoqian Jiang
Developing slow-release pesticides and reducing the migration of pesticides are the keys to improving the utilization efficiency of pesticides. Here, phosphorus-modified (P-WBC) and subsequent ammonia-modified biochar (NP-WBC) were prepared using pine tree sawdust at different pyrolysis temperatures (300 °C, 500 °C, and 700 °C), while the adsorption and desorption capacities of P-WBC and NP-WBC on 2,4-dichlorophenoxyacetic acid (2,4-D) were compared, as well as their intrinsic mechanism disparity. It was found that pyrolysis temperature, phosphorus- and ammonia-modification all improved the adsorption and slow-release of 2,4-D from biochar significantly through enriching the surface morphology, pore structure and optimizing the composition of functional groups of biochar. The adsorption mechanisms involved pore filling, hydrogen bonding, π-π interactions and phosphorus/nitrogen (P/N)-containing groups bonding, while the release behavior was predominantly governed by Fickian diffusion. The main active reaction sites of P-WBC and NP-WBC were the edge-located phosphate-like functional groups (C-O-P1) and pyridinic N, respectively, and C-O-P1 had the highest affinity for 2,4-D, as corroborated by density functional theory (DFT) calculation. Compared to NP-WBC, P-WBC exhibited greater 2,4-D adsorption and slow-release capacities, which was attributed to the stronger hydrogen bonding and P-containing functional groups bonding of C-O-P1 with 2,4-D. In particular, 500 °C P-WBC demonstrated exceptional thermal stability, great 2,4-D adsorption capacity (75.27 mg/g), and the most effective slow-release performance (9.34%). In addition, further germination experiments showed that 500 °C P-WBC loaded with 2,4-D (500 °C P-WBC@2,4-D) exhibited equivalent herbicidal efficacy to commercially available 2,4-D while also reducing the toxicity of the pesticide to maize to a certain extent. This work offers an avenue for the synthesis to prepare biochar as a carrier for slow-release pesticides under suitable pyrolysis temperature and modification method, improve pesticide efficiency, and reduce environmental pollution.
研究领域:
环境科学
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农业化学
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材料科学
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植物科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）