JOURNAL OF FOOD SCIENCE
·
2023
·Vol. 88
·期 3
·977-987
·
IF 3.9
Wenfu Hou,Yile Lin,Yujie Zhang,Tingting Liu,Juan Du,Yang Yi,Hongxun Wang
This study investigated the effect of 0.8% (m/v) kojic acid treatment on changes in the microstructure and myofibrillar protein of duck meat covered with oxygen-permeable polyvinylchloride (PVC) film (9 ± 0.5 µM) during superchilled storage (−1.65 ± 0.5°C). The superchilled samples exhibited wider gaps between muscle fibers at 5 weeks storage compared with kojic acid–treated groups. Based on the variation of water status, the water-holding capacity decreased significantly ( p < 0.05), and bound water and immobilized water were gradually converted into free water during superchilled storage. For kojic acid–treated samples, however, no major changes were observed with respect to muscle structure, water status, and protein degradation at 6 weeks. The 0.8% (m/v) kojic acid treatment increased the water-holding capacity, reduced carbonyl content and protein degradation, and decreased the α-helix contents loss of myofibrillar proteins. Kojic acid treatment effectively protected myofibrillar protein structure from being destroyed during superchilled storage, suggesting that this method was a good way to reduce protein oxidation and prolonged its shelf life.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
·
2023
·Vol. 27
·期 16
·2362-2371
·
IF 5.3
Zhang-Yong Ren,Qiao Wu,Bing Pan,Jia-Zong Liu,Qiang He,Ren Lang,Shao-Cheng Lyu
Allogeneic blood vessels are regarded as one of the best natural substitutes for diseased blood vessels due to their good vascular compliance and histocompatibility. Since the supply and demand of allograft blood vessels do not always match in time and space, a good preservation scheme for isolated blood vessels is essential. The abdominal aortas of 110 male Sprague–Dawley (SD) rats were randomly divided into three groups, including cold storage group (4°C) (CSG), frozen storage group (FSG) and ambient storage group (25 ± 2°C) (ASG). Seven time points of preservation for 1, 3, 5, 7, 14, 30 and 90 days were set for detection. The changes in vascular physiological function were evaluated by MTT test and vasoconstriction ability detection, and the changes in vascular wall structure were evaluated by the tension tolerance test and pathological staining. The vascular function of CSG was better than FSG within first the 7 days, but the result was opposite since the 14th day. The vascular wall structure, collagen and elastic fibres of vessels, in CSG, showed oedema within 30 days, and continuous disintegration and rupture at 90 days. The vessel wall structure of FSG remained intact within 90 days. The tensile strength of the vessels in CSG was better than that in FSG within 5 days, and there was no statistical difference between the two groups between the 7th and 30th day, and then, the FSG was higher than CSG on the 90th day. Both cold storage and frozen storage could be applied as safe and effective preservation schemes for isolated rat artery within first 30 days. Cold storage is recommended when the storage time is <14 days, and then, frozen storage is better.
研究领域:
生理学
·
医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2019
·
IF 3.714
Yinxia Chen,Long Sheng,Mostafa Gouda,Meihu Ma
The effects of ultrasonic treatment (180 W, 25 min) on the foaming and physicochemical properties of egg white from shell egg stored for seven periods (0, 15, 30, 45, 60, 75 and 90 d) were investigated in this study. The foaming ability significantly increased after ultrasound treatment and the highest foaming ability (99.13%) was achieved at 60 d compared to untreated egg white (29.75%). This improvement was ascribed to the increased free sulfhydryl content and surface hydrophobicity, indicating that egg white protein became easier to adsorb at the air-water interface and its structure was more flexible. However, a reduction in the foaming stability of ultrasound-treated egg white resulted from the lower absolute zeta potential. Moreover, decreasing particle size and broadening protein size distribution were obtained after ultrasonic processing. Using scanning electron microscopy, small aggregates were observed on the surface of the untreated sample after storage, while the ultrasound-treated sample had large numbers of irregular and unevenly distributed pores with different sizes. These results provide important evidence of the protein properties induced by ultrasound modification during storage, and further expand the ultrasound application in the food storage industry.
研究领域:
食品科学
·
蛋白质化学
✓ 已验证
DOI
高影响因子
Journal of Energy Storage
·
2023
·
IF 9.4
Rong Li,Zhigao Sun
The motivation of this study is to investigate the effect of side chain groups of amino acids on hydrate formation . To confirm their effects on hydrate nucleation and growth, we use aromatic amino acids (tryptophan, phenylalanine, tyrosine), heterocyclic amino acids (histidine, proline) and aliphatic acids (arginine, alanine) as biological additives. The effect of different side chain groups of amino acids on hydrate formation kinetics is investigated. It is found that the hydrophobic groups of amino acids have a significant influence on promoting hydrate formation compared with the hydrophilic groups . Among the hydrophobic groups, aromatic groups are superior to aliphatic groups in improving hydrate nucleation and growth rate, while aliphatic groups are more advantageous in increasing hydrate production, cold storage capacity (CSC) and hydrate conversion rate. Heterocyclic amino acids have no obvious effect on hydrate formation. In addition, foam is not produced during hydrate dissociation , which is good for practical application.
研究领域:
生物学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
DYES AND PIGMENTS
·
2019
·
IF 4.018
Jinjin Guo,Xiaoyu Wang,Shuxian Zhu,Lu Liu,Xiaofeng Jiang,Dongdong Ban,Lidong Li
We combined the unique natural oxygen transporting function of red blood cells (RBCs) with photosensitive nanocomposites to create biomaterials with strong singlet oxygen ( 1 O 2 ) generation. During cold storage for a long time, structural changes to RBCs may occur, which reduce their oxygen carrying capacity. The nanocomposite developed herein featured dual emission characteristic, which enabled evaluation of the status of the RBCs. On the basis of energy transfer from the conjugated polymer to iridium(III) complexes, the formed nanocomposites show intense, long-lived and oxygen-sensitive emission. When modified with 1,2-distearoyl- sn -glycero-3-phosphoethanolamine- N -[methoxy(polyethyleneglycol)-2000], the nanocomposites non-invasively entered RBCs through hydrophobic interactions . We measured the quenching effect of oxygen on the emission of the iridium(III) complexes. The differential fluorescent signals and fluorescence lifetime images indicated the storage history of the RBCs. RBCs in good condition stored oxygen. When engineered with nanocomposites, RBCs generated large amounts of cytotoxic 1 O 2 upon irradiation. These decorated RBCs might serve as a new kind of photosensitive biomaterial.
研究领域:
生物材料学
·
医学工程
·
光动力治疗
✓ 已验证
DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2019
·
IF 6.361
Jiaojiao Zhao,Jinling Long,Yongqiang Du,Jiankui Zhou,Yadong Wang,Zipeng Miao,Yingliang Liu,Shengang Xu,Shaokui Cao
Recyclable low-temperature phase change microcapsules (LTPCMs) have the potential applications in the short-distance cold chain transportation due to their reliable reusability in cold storage . Herein, LTPCMs are synthesized via in-situ suspension copolymerization of styrene and methyl methacrylate in absence of harm substances, providing the non-crosslinking copolymer shells. n -Dodecane, n -tridecane and n -tetradecane, inducing the microphase separation of non-crosslinking copolymers, are successfully encapsulated to achieve n -do-LTPCMs, n -tri-LTPCMs and n -tetra-LTPCMs, which respectively bear the high phase change enthalpy of 110.53 J·g −1 at −8.69 °C, 38.33 J·g −1 /93.71 J·g −1 at −17.61 °C/−4.96 °C and 166.79 J·g −1 at 8.59 °C and subsequently show the cold-discharging periods of 30 min, 40 min and 120 min. The multiple circulation of cold-discharging process indicates the excellent recyclability for cold storage owing to their unchanged cold-discharging period. Especially, n -tetra-LTPCM-65 bears the best comprehensive cold-storing performance in all the previously reported LTPCMs, such as narrow cold-discharging temperature range of 3–4 °C, long cold-discharging period of 69–120 min and low cold-discharging capacity of 33.4 J·g −1 ·K −1 . This work successfully provided the recyclable LTPCMs for cold storage in the short-distance cold chain transportation.
研究领域:
材料科学
·
能源存储
·
化学工程
✓ 已验证
DOI
高影响因子
JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY
·
2018
·
IF 0.715
Ti Wu,Shoukai Lin,Bisha Wu,Yu Chen,Suying Lin,Dahe Lin,Linxia Chen,Zhisheng Zhang,Jincheng Wu
Cold-stored fruits of the loquat (Eriobotrya japonica Lindl.) plant suffer from strong lignification, which leads to a hard texture and a crude mouthfeel of the pulp. However, the signalling mechanisms of loquat fruits under cold storage remain unclear. In this study, the heterotrimeric G protein α subunit (Gα) gene (EjLGA1) from loquat was isolated, and in silico analysis was performed. The levels of EjLGA1 expression in cold-stored loquat fruits with different pretreatments were also investigated. The largest open-reading frame of EjLGA1 is 1173 bp, encoding a polypeptide of 390 amino acids. The nucleotide and amino acid sequences of the Gα subunit are highly conserved among loquat and 48 other seed plants, indicating that the loquat Gα subunit likely has similar functions in other plants. A reliable model of the loquat Gα subunit was constructed, which revealed the structure of the subunit. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) results showed that EjLGA1 is highly expressed under cold storage, which suggests the significance of EjLGA1 function in loquat fruits in response to cold stress. However, ethephon and 1-methylcyclopropene (1-MCP) pretreatments did not regulate EjLGA1expression. These results provide new insight into the signalling mechanism in cold-stored loquat fruits.
研究领域:
植物学
·
分子生物学
·
食品科学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Juxin Pei,Jun Mei,Gan Wu,Huijie Yu,Jing Xie
The active coatings supplemented with epigallocatechin gallate (EGCG) (0.16 %, 0.32 %, and 0.64 %, respectively) combined with superchilling storage (−3 ± 0.2 °C) were used to reduce hydrogen peroxide (H 2 O 2 ) content, and inhibit lipid and protein oxidations of large yellow croaker during 42 days of superchilling storage. EGCG coatings delayed lipid and protein oxidations by inhibiting the generation of H 2 O 2 , malondialdehyde (MDA) and carbonyl groups, and maintaining a higher Ca 2+ -ATPase activity and sulfhydryl content. We also observed that EGCG treatments maintained myofibrillar organized secondary structure by keeping higher α-helix content, and also stabilized tertiary structure during superchilling storage. Low-field nuclear magnetic resonance (LF-NMR) revealed that EGCG treatments might improve the association of water molecules with protein for fixed water. In addition, sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) and scanning electron microscope (SEM) images both showed that these treatments could delay the myofibrillar degradation of fresh fish. Overall, we report that the active coatings containing EGCG treatments protect the lipid and protein of large yellow croaker during superchilling storage.
研究领域:
食品科学
·
水生生物学
·
食品保鲜
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Bio Materials
·
2018
·
IF 0
Xingzhong Zhang,Yixiang Wang,Xiaogang Luo,Ang Lu,Yan Li,Bin Li,Shilin Liu
The increased quantities of fat in plants could allow the cells to inhibit the growth of ice and thus prevent the damages of their tissue structure in winter. In view of the structural buildup of freezing tolerance mechanism, here we presented a facile way of employing O/W Pickering emulsion as a template to produce the freestanding organo-hydrogels with increased mechanical stability and energy storage capacity. The oil droplets stabilized by cellulose nanofibrils were dispersed in the alginate polymer network that cross-linked with Ca2+, which resulted in homogeneous and closely packed microstructures. The prepared organo-hydrogels could maintain original gel structure under frozen conditions and had extraordinary mechanical performance. It could endure compressive stress up to 35 KPa (at 50% strain) and the elastic modulus was around 72 KPa, while the solid content of polysaccharides was only about 0.75%. By using our comprehensive strategy, organo-hydrogels with higher volumes of oil phase exhibited an enhanced cold storage capacity. For alginate hydrogel, it took 8 min when the temperature rose from 0 to 5 °C, while for the organo-hydrogel with oil volume of 30%, it took about 24 min. After 34 min, the inner temperature of alginate hydrogel was close to 25 °C, and about 70 min were needed for the temperature of organo-hydrogel to reach 25 °C. This kind of gel materials with complementary heteronetworks not only will have potential applications in cold chain logistics, but also can be applied in other fields with unusual functions.
研究领域:
材料科学
·
化学工程
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
Food Quality and Safety
·
2023
·
IF 5.6
Xu Zhilong,Pei Juxin,Mei Jun,Yu Huijie,Chu Siyuan,Xie Jing
The effect of gum tragacanth (GT) and sodium alginate (SA) active coatings incorporated with epigallocatechin gallate (EGCG, 0.16%, 0.32% and 0.64%, respectively) on the quality of large yellow croaker fillets was evaluated during superchilling storage at -3 ℃ for 42 days. The results revealed that the GT-SA-EGCG active coatings delayed the microbial spoilage via reducing the numbers of total viable counts, Pseudomonas spp. and psychrophiles bacteria, retarded degradation of the physical properties of water holding capacity, cooking loss, color, odor and texture. GT-SA-EGCG coatings could also maintain the fillets’ freshness by keeping lower total volatile basic nitrogen, K value and histidine content, inhibiting myofibrillar protein degradation during superchilling storage. These results suggested that the active coatings embedded with GT-SA-EGCG could be used as a preservative to enhance the quality of large yellow croaker and prolong the shelf life by 7-14 days during superchilling storage at -3 ℃.
研究领域:
食品科学
·
食品保鲜
·
水生生物学
·
渔业
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）