BIOSENSORS & BIOELECTRONICS
·
2021
·
IF 10.618
Jiahao Li,Xuping Wu,Yue Li,Xin Wang,Huachuan Huang,Dan Jian,Yanke Shan,Yue Zhang,Chengcheng Wu,Guolei Tan,Shouyu Wang,Fei Liu
Classical gold standard HBV detection relies on expensive devices and complicated procedures, thus is always restricted in large-scale hospitals and centers for disease control and prevention. To extend HBV detection to primary clinics especially in underdeveloped areas, we design amplification-free smartphone-based attomolar HBV detecting technique based on single molecule sensing. Verified by synthesized HBV target DNA , this technique reaches a detection limit at attomolar concentration (100 aM); and verified by 110 clinical samples, it also reaches a rather high sensitivity of 10 4 copy/mL (≈2000 IU/mL) with a high accuracy of 93.64% certificated by gold standard HBV detecting devices. Besides, this technique can quantify HBV viral load in 70 min only using portable and inexpensive devices as well as simple operations. Because of its cost-effective, field-portable and operable design, highly sensitive and selective detecting capability and wireless data connectivity, this technique can be potentially used in mobile HBV diagnoses and share HBV epidemic information especially in resource limited situations.
研究领域:
医学检验
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
Sensors & Diagnostics
·
2022
·Vol. 2
·期 1
·168-175
·
IF 0
Lingfeng Xu,Yuefeng Wang,Chuan Jiang,Yanrong Huang,Genhe He,Limin Liu
Liquid safety has become one of the urgent affairs in global public health studies, and a noninvasive and effective viscosity inspection method is expected to ease the burden of continuously increasing health problems. In this study, one kind of activatable molecular sensor (DTPMP) based on a salicylaldehyde derivate has been synthesized via the Schiff base condensation reaction. This sensor is comprised of a donor–acceptor conjugated structure. We proposed the viscosity of the liquid microenvironment as a marker, and a rotatable conjugate structure was utilized as the recognition site. The molecular sensor was synthesized in a one-step facile way, and DTPMP displayed a longer emission wavelength and larger Stokes shift (142.5 nm in water, 116.8 nm in glycerol), which endows the sensor with the capacity of avoiding auto-fluorescence and achieving high signal-to-noise ratio imaging. The rotation of aromatic rings in DTPMP is restricted in a high-viscosity microenvironment, with the gradually increasing fluorescence emission signal at 532 nm. Moreover, the DTPMP sensor exhibits high adaptability, selectivity, sensitivity, and good photostability in various commercial liquids. Significantly, this sensor has successfully been applied not only in the determination of the thickening effects of food gum but also in the detection of viscosity enhancement during the liquid food spoilage process, which may be helpful in screening the metamorphic stage. We expected that this unique strategy will reinvigorate the continued perfection of the fluorescence technique toward liquid safety investigation.
研究领域:
分析化学
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生物医学工程
·
公共健康
✓ 已验证
DOI
ANALYTICA CHIMICA ACTA
·
2023
·
IF 6.2
Jing Fu,Junxiao Liu,Yanyu Li,Chen Wang,Jinglin Shen,Wei Qi
Ultrasmall gold nanoclusters (NCs) have been engineered as a new kind of functional material due to their excellent photoluminescence properties. However, the synthesis of highly luminescent water-soluble nanoclusters with near-infrared (NIR) emission remains limited. Herein, we developed a pH-regulated strategy to facilitate the construction of self-assemblies with enhanced luminescence based on aggregation-induced emission (AIE) strategy. Using 2-mercaptobenzoic acid (MBA) as reductant and stabilizer, the original weakly luminescent AuNCs exhibited intense emission by adjusting pH controllably. The formation of compact organized nanostructures could effectively restrict the rotation and vibration of capping ligands by non-covalent interactions, which reduced the nonradiative relaxation from excited states and finally improved the emission properties of AuNCs. Moreover, the assemblies possess many intriguing features including bright NIR luminescence and excellent biocompatibility, which could be used as luminous probes in biological molecules sensing (tyrosinase (TYR) and dopamine (DA)) and promising candidates for cell imaging . This study provides a simple and feasible strategy for developing metal NCs-based smart optical materials in the field of bioscience.
研究领域:
生物化学
·
纳米生物学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Tingting Luo,Yiqi Li,Yanxue Xu,Shiting Zhang,Yujue Wang,Xingming Kou,Dan Xiao
In this article, a hyperfluorescence 2-pyridone derivative (D-TPCA) has been synthesized within 5 min by the microwave-assistant heat treatment of the aqueous mixture of citric acid and 2-aminoethanethiol hydrochloride. The as-prepared D-TPCA exhibited good aqueous solubility and excellent fluorescent property with a quantum yield of 89.9%. Because the fluorescence of D-TPCA could be effectively quenched by picric acid (PA), D-TPCA could be served as a “turn-off” fluorescence sensor for PA in aqueous solution. The proposed sensing method permitted a selective and sensitive detection of PA in a linear range of 0–45 μM with a detection limit of 56.0 nM. The determination results of PA in real water samples with a recovery range of 95.4–107.3% confirmed a good analytical performance of this sensor. In addition, the possible sensing mechanism has been discussed in detail.
研究领域:
分析化学
·
材料科学
✓ 已验证
DOI
高影响因子
Device
·
2025
·
IF 8
Xinyi Ke,Yifan Duan,Yifei Duan,Zhe Zhao,Chunyu You,Tingting Sun,Xingyu Gao,Ziyu Zhang,Wen Xue,Xuanyong Liu,Yongfeng Mei,Gaoshan Huang,Junhao Chu
Electronic skin (e-skin) mimics the sensing abilities of human skin and offers sensitivity and flexibility, which has garnered attention for medical health monitoring. Nonetheless, limitations in the properties of active materials and signal-to-noise ratio hinder the implementation of multi-functional detection such as integrating biomolecular sensing, motion detection, and electrocardiography in a single device. Here, we introduce a strategy of preparing a multi-functional e-skin that utilizes a composite material combining self-locking chitosan and conductive metal-organic framework film, achieving high-performance motion detection and biomolecular sensing. The integration of deep learning, specifically transformer neural networks, aids in recognizing subtle facial micro-expressions. A sensing array is crafted by utilizing a rapid assembly method that supports discerning more attributes with heightened precision.
研究领域:
生物医学工程
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材料科学
·
人工智能
✓ 已验证
产品关联
DOI
高影响因子
Materials Horizons
·
2024
·
IF 12.2
Dezhi Jiao,Sihan Gu,Li Cheng,Shuoqi Li,Chengbao Liu
Visually monitoring micro-crack initiation and corrosion failure evolution is crucial for early diagnosis of structural health and ensuring safe operation of infrastructures. However, existing damage detecting approaches are subject to the limited-detection of heterogeneous structures, intolerance of harsh environments, and challenge of quantitative analysis, impeding applications in structural health monitoring (SHM). Herein, we present a stretchable, semi-quantitative, instrument-free, supramolecular SHM sensor by integrating a polyurea elastomer with sensitive corrosion-probes, enabling localized corrosion monitoring and quantification of failure dynamics. Initially, a correlation between visual monitoring signals and structural health status is proposed, and sensor-based image processing software that accurately quantifies structural failure indicators (crack scale, corrosion reactivity and deterioration status) is proposed. Moreover, this sensor can be fabricated as multiple derivatives: a coating or patch covered on metallic substrates and an ionic-responsive test strip, ensuring real-time detection of the initiation of pitting, degradation events of metallic components and convenient monitoring of ion concentrations in corrosive media. Furthermore, the inherent geometric plasticity and dynamic hydrogen-bonded network validates the reliability for heterogeneous components and stability under extreme environments of sensors. This portable, smart SHM strategy established the channel-transformation model from corrosion dynamics to visual signals, exhibiting prospects for structural monitoring in offshore energy-harvesting equipment.
研究领域:
材料科学
·
工程学
·
化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2018
·Vol. 42
·期 21
·17317-17322
·
IF 3.201
Chang Liu,Peng Wang,Xueke Liu,Xiaotong Yi,Zhiqiang Zhou,Donghui Liu
The quaternary ammonium herbicides mainly include paraquat and diquat, whose fast and simultaneous detection is important for environmental and public safety. Herein, we simultaneously analyzed paraquat and diquat by a simple three-component supramolecular sensor array, which was simply prepared from carboxymethyl-β-cyclodextrin (CM-β-CD) and fluorophores. Under the optimal pH, the two herbicides were excellently distinguished in the principal component analysis (PCA) score plot in the presence of various interferences. Then, the results of semi-quantitative analysis through linear discrimination analysis (LDA) also showed good cluster separation for both paraquat and diquat in the concentration range of 10–250 μM. Moreover, in order to test the unknown samples, the support vector machine (SVM) regression model was applied for simultaneous quantitative analysis of paraquat and diquat, which was successful in the prediction of the amount of the two analytes. Accordingly, this simple supramolecular sensor array is expected to be applied in the rapid and accurate analysis of quaternary ammonium herbicides.
研究领域:
分析化学
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环境科学
·
生物传感
✓ 已验证
产品关联
DOI
高影响因子
Nanoscale
·
2023
·Vol. 15
·期 6
·2779-2787
·
IF 6.7
Leilei Lan,Xingce Fan,Caiye Zhao,Juan Gao,Zhongwei Qu,Wenzhe Song,Haorun Yao,Mingze Li,Teng Qiu
As an emerging class of two-dimensional (2D) materials, MBenes show enormous potential for optoelectronic applications. However, their use in molecular sensing as surface-enhanced Raman scattering (SERS)-active material is unknown. Herein, for the first time, we develop a brand-new high-performance MBene SERS platform. Ordered vacancy-triggered highly sensitive SERS platform with outstanding signal uniformity based on a 2D Mo4/3B2 MBene material was designed. The 2D Mo4/3B2 MBene presented superior SERS activity to most of the semiconductor SERS substrates, showing a remarkable Raman enhancement factor of 3.88 × 106 and an ultralow detection limit of 1 × 10−9 M. The underlying SERS mechanism is revealed from systematic experiments and density functional theory calculations that the ultrahigh SERS sensitivity of 2D Mo4/3B2 MBene is derived from the efficient photoinduced charge transfer process between MBene substrates and adsorbed molecules. The abundant electronic density of states near the Fermi level of 2D Mo4/3B2 MBene enables its Raman enhancement by a factor of 100 000 times higher than that of the bulk MoB. Consequently, the 2D Mo4/3B2 MBene could accurately detect various trace chemical analytes. Moreover, with ordered metal vacancies in the 2D Mo4/3B2 MBene, uniform charge transfer sites are formed, resulting in an outstanding signal uniformity with a relative standard deviation down to 6.0%. This work opens up a new horizon for the high-performance SERS platform based on MBene materials, which holds great promise in the field of chemical sensing.
研究领域:
材料科学
·
光学
·
化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2020
·
IF 3.232
Mei-Xiang Yang,Qing Tang,Mei Yang,Qin Wang,Zhu Tao,Xin Xiao,Ying Huang
The host–guest inclusion complexes that comprise an inverted cucurbit[7]uril ( i Q[7]) and a quinoline derivative, 4-(4-dimethylaminostyryl) quinoline (DSQ) at different pHs were exploited as multiple supramolecular sensors to sense l -α-amino acids. DSQ complexation inside i Q[7] at different pHs leads to increased fluorescence and formation of different-colored i Q[7]-DSQ complexes. The enhanced fluorescence of DSQ after i Q[7] encapsulation may be attributed to limited dimethylamine rotation and the formation of a twisted internal charge transfer (TICT) state. The DSQ@ i Q[7] sensors have different affinities for l -α-amino acids at different pHs. Therefore, we propose a pH-stimulus response supramolecular sensor for the discrimination of structurally similar l -α-amino acids in aqueous solution.
研究领域:
分析化学
·
超分子化学
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产品关联
DOI
PubMed
高影响因子
Biosensors-Basel
·
2023
·Vol. 13
·期 9
·875
·
IF 5.4
Ziang Wu,Roujuan Kou,Kun Ni,Rui Song,Yuxuan Li,Tianhu Li,Hao Zhang
DNA ligases are essential enzymes involved in DNA replication and repair processes in all organisms. These enzymes seal DNA breaks by catalyzing the formation of phosphodiester bonds between juxtaposed 5′ phosphate and 3′ hydroxyl termini in double-stranded DNA. In addition to their critical roles in maintaining genomic integrity, DNA ligases have been recently identified as diagnostic biomarkers for several types of cancers and recognized as potential drug targets for the treatment of various diseases. Although DNA ligases are significant in basic research and medical applications, developing strategies for efficiently detecting and precisely quantifying these crucial enzymes is still challenging. Here, we report our design and fabrication of a highly sensitive and specific biosensor in which a stable DNA hairpin is utilized to stimulate the generation of fluorescence signals. This probe is verified to be stable under a wide range of experimental conditions and exhibits promising performance in detecting DNA ligases. We anticipate that this hairpin-based biosensor will significantly benefit the development of new targeting strategies and diagnostic tools for certain diseases.Keywords:DNA ligases;extraordinarily stable hairpins;biomarker detection;fluorescence probes;molecular sensing
研究领域:
分子生物学
·
医学诊断
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYST
·
2022
·Vol. 147
·期 18
·4132-4140
·
IF 5.227
Lingfeng Xu,Fangzhi Xiong,Minqing Kang,Yanrong Huang,Kui Wu
Viscosity is one of the most important physical parameters in a liquid, noninvasive, and effective viscosity inspection method toward liquid safety that needs to be developed urgently. In this study, two kinds of novel molecular sensors, namely, DPBID and DPTMID, were strategically constructed by the triphenylamine indanedione derivates; the rotatable conjugate structure was utilized as the recognition site and fluorescence quencher. This couple of molecular sensors was synthesized in a one-step facile manner. DPTMID displayed longer emission wavelength and larger Stokes shift (195 nm in water, 138 nm in glycerol) with a narrower energy band. Moreover, DPTMID exhibited high selectivity, sensitivity, and significant fluorescence signal enhancement toward a higher viscous microenvironment. The molecular sensor displayed good photostability, selectivity, and universality in various commercial liquids and featured with typical aggregation-induced emission (AIE). With the aid of DPTMID, the thickening effects of liquid thickeners can be captured. More importantly, DPTMID was explored to visualize the viscosity fluctuations during the metamorphic stages of liquids, and it was found that the microenvironment viscosity level is closely related to the spoilage degree of liquids. The method with rapid detection, high sensitivity, cheap equipment, and fast results output toward food quality and safety inspection can be achieved through this study.
研究领域:
化学
·
材料科学
·
食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
DYES AND PIGMENTS
·
2022
·
IF 5.122
Meijuan Zhang,Jian Cao,Cuiping Huang,Mingming Liu,Yanan Li,Chuanxiao Wang,Yajing Tu
Human serum albumin (HSA) accounts for 60% of total plasma protein and is closely related to life-threatening diseases. In this strategy, three fluorescent sensors of Nap-OH, Nap-C and Nap-NO 2 based on twisted intramolecular charge transfer (TICT) are designed and synthesized for detecting of HSA, and the effect of electron donating-withdrawing groups of sensors on the detection of HSA is studied. After added HSA, the fluorescent emission of Nap-NO 2 is turned on at 630 nm, and the color changed from colorless to pink. Nap-NO 2 is characterized by a 14.06-fold increase in fluorescence emission and has high sensitivity, good selectivity, fast response, and good stability. Nap-NO 2 could effectively distinguish HSA from other proteins, including bovine serum albumin (BSA). Drug competition experiments are performed that Nap-NO 2 can bind to the IB domain of HSA, avoiding the interference of commonly used drugs. Nap-OH, Nap-C and Nap-NO 2 can also be used for viscosity detection.
研究领域:
生物化学
·
医学检测
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）