JOURNAL OF RAMAN SPECTROSCOPY
·
2016
·Vol. 48
·期 3
·443-447
·
IF 2.395
Yanna Yang,Fu Wan,Pinyi Wang,Xiping Zeng,Yunpeng Jia,Haiyang Shi,Weigen Chen,Yingzhou Huang
For the induced image charge on surface, the metal film as a substrate brings the metal nanostructures some novel surface plasmon-related phenomenon, e.g. electromagnetic field redistribution and energy focusing. In this work, the surface-enhanced Raman scattering in sandwich structure consist of Au nanoplate and Au film adsorbed with molecule monolayer is investigated. The surface-enhanced Raman scattering spectra point out that the shielded Raman signals of molecule under metal nanoplate could be collected from this metal-encapsulated sandwich structure with the help of nanoparticle. This interesting phenomenon is further confirmed by the simulated electromagnetic field where the light power in the gap between Au nanoplate and Au film is greatly enhanced with a nanoparticle located. The further results also demonstrate this nanoparticle assisted Raman information acquisition is highly influenced by the thickness of encapsulated nanoplate and size of nanoparticle. Furthermore, the positive results of nanoparticles with different shapes in this interesting phenomenon provide an extensive application prospects including surface-enhanced Raman scattering detection, light information storage and selective harvesting, location detection of light information, photocatalysis, etc. Copyright © 2016 John Wiley & Sons, Ltd.
研究领域:
光学
·
材料科学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
Nanoscale
·
2024
·
IF 5.8
Junqiu Liu,Zhaidong Liu,Chunqin Zhao,Yuting Jiao,Baohong Li,Jiaju Shi,Zichao Chen,Zhen Zhang
Exosomal programmed death ligand-1 (ExoPD-L1) is a vital marker of immune activation in the early stages of tumor therapy and it can inhibit anti-tumor immune responses. However, due to the low expression of ExoPD-L1 in cancer cells, it is difficult to perform highly sensitive assays and accurately differentiate cancer sources. Therefore, we constructed a coaxial dual-path electrochemical biosensor for highly accurate identification and detection of ExoPD-L1 from lung cancer based on chemical–biological coaxial nanomaterials and nucleic acid molecular signal amplification strategies. The measurements showed that the detected ExoPD-L1 concentrations ranged from 6 × 102 particles per mL to 6 × 108 particles per mL, and the detection limit was 310 particles per mL. Compared to other sensors, the electrochemical biosensor designed in this study has a lower detection limit and a wider detection range. Furthermore, we also successfully identified lung cancer-derived ExoPD-L1 by analyzing multiple protein biomarkers expressed on exosomes through the “AND” logic strategy. This sensor platform is expected to realize highly sensitive detection and accurate analysis of multiple sources of ExoPD-L1 and provide ideas for the clinical detection of ExoPD-L1.
研究领域:
生物医学工程
·
癌症生物学
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电化学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Oncology
·
2021
·
IF 6.244
Xu Ying,Lu Lunjie,Luo Judong,Wang Lili,Zhang Qi,Cao Jianping,Jiao Yang
The prognosis of pancreatic cancer remains very poor worldwide, partly due to the lack of specificity of early symptoms and innate resistance to chemo-/radiotherapy. Disulfiram (DSF), an anti-alcoholism drug widely used in the clinic, has been known for decades for its antitumor effects when simultaneously applied with copper ions, including pancreatic cancer. However, controversy still exists in the context of the antitumor effects of DSF alone in pancreatic cancer and related mechanisms, especially in its potential roles as a sensitizer for cancer radiotherapy. In the present study, we focused on whether and how DSF could facilitate ionizing radiation (IR) to eliminate pancreatic cancer. DSF alone significantly suppressed the survival of pancreatic cancer cells after exposure to IR, both in vitro and in vivo. Additionally, DSF treatment alone caused DNA double-strand breaks (DSBs) and further enhanced IR-induced DSBs in pancreatic cancer cells. In addition, DSF alone boosted IR-induced cell cycle G2/M phase arrest and apoptosis in pancreatic cancer exposed to IR. RNA sequencing and bioinformatics analysis results suggested that DSF could trigger cell adhesion molecule (CAM) signaling, which might be involved in its function in regulating the radiosensitivity of pancreatic cancer cells. In conclusion, we suggest that DSF alone may function as a radiosensitizer for pancreatic cancer, probably by regulating IR-induced DNA damage, cell cycle arrest and apoptosis, at least partially through the CAM signaling pathway.
研究领域:
肿瘤学
·
药理学
·
放射生物学
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分子生物学
✓ 已验证
DOI
PubMed
高影响因子
Small
·
2022
·Vol. 18
·期 36
·2201656
·
IF 15.153
Yang Sun,Qianrong Zhou,Yawei Du,Jian Sun,Wei Bi,Wenjuan Liu,Ruixue Li,Xingwen Wu,Fei Yang,Liang Song,Ni Li,Wenguo Cui,Youcheng Yu
Bone defect regeneration depends on the population and lifespan of M2 macrophages, which are regulated by dual signals generated by the “physical” spatial configuration of biological tissues and “molecular” chemokines. Herein, inspired by the reprogramming of macrophages, immunoengineered porous microspheres are constructed to accelerate bone repair through the regulation of both “physical” and “molecular” signals. The porous structure of injectable poly ( l -lactic acid) (PLLA) microspheres prepared by the microfluidic technique provides a “physical signal” for osteogenic differentiation. Additionally, interleukin (IL)-4-loaded liposomes (Ls) are modified on PLLA microspheres through amide bonds to produce IL-4/Ls/PLLA microspheres, providing a “molecular signal” in stimulating the differentiation of macrophages to M2 type. It is confirmed that IL-4/Ls/PLLA microspheres could induce M2-macrophages polarization and potentiate osteoblast proliferation and differentiation while coculturing with macrophages and osteoblasts in vitro. Besides, IL-4/Ls/PLLA microspheres are proved to promote bone defect regeneration by inducing the conversion of M1 macrophages to M2 through dual biosignal-functional regulation in both the calvaria defect and maxillary sinus defect models. Overall, the immuno-reprogrammed IL-4/Ls/PLLA microspheres achieve the precise immuno-reprogramming of macrophages by dual biosignal-functional regulation. This immune reengineering strategy paves a way for clinical bone defect treatment.
研究领域:
组织工程
·
生物材料
·
免疫学
·
再生医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）