INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
·
IF 8.5
Kai Yang,Zhenjie Tang,Chong Zhang,Ruide Zhang,Sheng Hu,Changjiang Lv,Jun Huang,Jiaqi Mei,Weirui Zhao,Lehe Mei
Salvianic acid A (SAA) is a catechol compound known for its diverse physiochemical functions and has significant applications in the food and pharmaceutical industries. 4-Hydroxyphenylacetate-3-hydroxylase (4HPA3H) is a critical enzyme for SAA biosynthesis, and improving its activity towards p -hydroxyphenyllactate acid (4HPLA) is essential for highly efficient SAA production in stable biosynthetic pathways. To address this, the distal site and loops of the substrate pocket were modified to improve 4HPA3H catalytic activity towards 4HPLA using computer-aided molecular modification methods. As a result, we identified and mutated two critical sites in Ec HpaB, a 4HPA3H monooxygenase from Escherichia coli : T398, a substrate loop site, and M205, a distal site. The mutants M205F, T398S, and M205F/T398S enabled 2.51-, 2.07-, and 2.20-fold increases in catalytic efficiency ( k cat / K m towards 4HPLA), respectively. Molecular dynamics simulations showed that the overall decreased flexibility of the substrate pocket loop in Ec HpaB might help improve the enzyme's catalytic activity for 4HPLA. Owing to the T398 site in the substrate pocket coming from another monomer, molecular modification of Ec HpaB with multi-monomer interactions was also worthy of attention. These findings not only enhanced the biosynthetic efficiency of SAA but also provided insights into enzyme distal site modification and the engineering of multi-monomer enzymes.
研究领域:
生物化学
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酶工程
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计算生物学
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产品关联
DOI
PubMed
高影响因子
INTEGRATED FERROELECTRICS
·
2023
·
IF 0.7
Lu-Ping Chen,Zhen Zhang,Wang-Quan Zhang,Hai-Lin Cong,You-Qing Shen,Bing Yu
Malignant tumor is one of the most important causes of death in the world today. At present, the successful cure cases of tumor patients are mainly due to the timely treatment of tumors. Therefore, it has become one of the urgent problems for human beings to find tumors as soon as possible and how to completely eradicate them. The technology of simultaneous imaging and treatment through photoinitiation is a promising strategy to fight cancer. In the previous research, we developed a new small molecule p-QM2AF for photothermal therapy, but the low fluorescence efficiency (QY) in the NIR-II range made it necessary to modify it. In this paper, we obtained better imaging and photothermal conversion performance by grafting two 9- methylene nonadecanes onto the p-QM2AF sub-receptor.
研究领域:
生物医学工程
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肿瘤学
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材料科学
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产品关联
DOI
高影响因子
CHEMISTRY OF MATERIALS
·
2023
·
IF 8.6
Jenaidullah Batur,Zunbin Duan,Min Jiang,Rui Li,Yabo Xie,Xue-Feng Yu,Jian-Rong Li
Widespread applications of zeolites demand the optimization of crystal size and morphology. Crystal growth modifiers (CGMs) have been proved effective for zeolite rational design, but the additional chemicals raise environmental and safety concerns. Herein, the industry-ready technique of cold atmospheric-pressure plasma jet (CAPPJ) was employed to induce reactive plasma species as green and eco-friendly CGMs. The synthesis mixtures of three aluminosilicate zeolites with assorted Si/Al ratios (SAR), channel dimensionalities, and pore apertures (i.e., MFI, LTA, and LTL framework types) were first subjected to Ar-CAPPJ treatment for up to 30 min during the aging period. Apparent size and shape modifications were successfully achieved for the resulting crystals with the emergence of rounded surfaces implying surface roughening at the molecular level. For the silicoaluminophosphate SAPO-34 (CHA type), the introduction of plasma CGMs was found to propel a phase transformation, promoting the crystallization of SAPO-18 (AEI type) impurity with reduced framework density. Experimental observations seem to suggest that the classical model of CGMs attaching to selected crystal surfaces is less likely to happen. Whereas, the investigation of zeolite L dispersions revealed enhanced concentrations and altered physicochemical properties of soluble species and amorphous precursors, alluding to the potential functional mechanism (s) of plasma CGMs. As a plausible technique to generate green and versatile CGMs, the impacts of CAPPJ on zeolite crystallization are systematically investigated for the first time, promoting the understanding of classical and nonclassical crystallization phenomena during zeolite synthesis.
研究领域:
材料科学
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化学工程
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产品关联
DOI
高影响因子
INORGANIC CHEMISTRY COMMUNICATIONS
·
2020
·
IF 1.943
Qingxu Bu,Guan-E Wang,Gang Xu,Xiaojing Long,Yanzhi Xia
Various luminescent organic-inorganic metal halide hybrid materials focus on fluorescence modulation. The commonly method to tunes the luminescence is usually via either modifying the structure of the inorganic anionic or the cation moiety. We here show the first time to modulate the fluorescent emission by inserting neutral organic molecules. The synthesis, crystal structure and optical properties of three organic-inorganic hybrids, [HEV][BiCl 5 ] (1), [MV] 3 [Bi 4 Cl 18 ]·[C 16 H 10 ] (2) and [MV] 3 [Bi 4 Cl 18 ]·[C 20 H 12 ] (3) ([HEV] 2+ = N-proton-N′-ethyl-4,4′-bipyridinium, [MV] 2+ = N,N′-dimethyl-4,4′-bipyridinium) were demonstrated. The compounds contain MV 2+ cations, bismuth (III)-chloro anions and pyrene or perylene molecules achieve fluorescence modulation upon the insertion of neutral molecules.
研究领域:
材料科学
·
光学
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DOI
高影响因子
Journal of Environmental Sciences
·
2023
·
IF 6.9
Yang Jin,Tongyin Liu,Yanpeng Mao,Fan Li,Chun Hu
The adsorption of pollutants can not only promote the direct surface reaction, but also modify the catalyst itself to improve its photoelectric characteristics, which is rarely studied for water treatment with inorganic photocatalyst. A highly crystalline BiOBr (c-BiOBr) was synthesized by a two-step preparation process. Owing to the calcination, the highly crystalline enhanced the interface interaction between pollutant and c-BiOBr. The complex of organic pollutant and [Bi 2 O 2 ] 2+ could promote the active electron transfer from the adsorbed pollutant to c-BiOBr for the direct pollutant degradation by holes (h + ). Moreover, the pollutant adsorption actually modified c-BiOBr and promoted more unpaired electrons, which would coupling with the photoexcitation to promote generate more O 2 •– . The molecular modification effect derived from pollutant adsorption significantly improved the removal of pollutants. This work strongly deepens the understanding of the molecular modification effect from the pollutant adsorption and develops a novel and efficient approach for water treatment.
研究领域:
环境科学
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材料科学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF HEAT AND MASS TRANSFER
·
2020
·
IF 4.947
Yixin Wang,Changjun Zou,Wenjing Li,Yiduo Zou,Hanxiang Huang
As a hardware carrier for informatization development, data centers will generate huge heat flux density during operation. Liquid cooling technology is considered as a thermal management method to solve this problem. The development of heat transfer fluids is of paramount importance. In this experiment, TiO 2 nanoparticles were successfully modified by supramolecular β-CD, and the corresponding nanofluids were prepared by using the modified nanoparticles. The experimental results show that the nanofluids can be stabilized for more than 50 days, and the average particle diameter of the nanoparticles can be maintained at 38.9 nm after 50 days. At 60 °C, the thermal conductivity of the 0.1 vol% nanofluids is increased by 36.01% compared with the base fluid, and the thermal diffusivity is increased by 58.28%. At a heating rate of 10 °C/min, the average specific heat capacity of the nanofluids is 3.15 times of the air. These results indicate that the heat transfer fluid has tremendous heat transfer potential in data center cooling system applications.
研究领域:
材料科学
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热能工程
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产品关联
DOI
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
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2021
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IF 5.316
Hui Wang,Zhi Yang,Wei Guo,Yongqiang Ji,Yun Zhou,Jialin Dang,Minqiang Wang
Recently, SnO 2 is widely used as an attractive electron transport layer in n-i-p planar perovskite solar cells (PSCs) due to its wide bandgap, high transmission, high carrier mobility , favorable band alignment and low annealing temperature. However, a commercial SnO 2 colloid solution suffers from soft agglomerate under long-term storage. Produced film pinholes and oxygen vacancy defect can increase non-radiative recombination at the perovskite/SnO 2 interface limiting the power conversion efficiency (PCE) and stability. Here, amino acid (glycine and alanine) and ammonium salt (ammonium titanium fluoride) are employed to modify SnO 2 nanocrystals and passivate perovskite defects at SnO 2 /perovskite film. The PCE of glycine (GLY) modified device was 19.71% and hysteresis was suppressed compared with that of 16.21% for control device. After 15-day aging at 85% relative humidity (RH), the encapsulated GLY modified device and control device retain 83.58% and 74.52% of their initial efficiency, respectively. Finally a perspective on how to modify SnO 2 with functional molecules toward efficient and stable PSCs is provided.
研究领域:
材料科学
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能源技术
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DOI
高影响因子
Journal of Materials Chemistry B
·
2017
·Vol. 5
·期 14
·2671-2678
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IF 4.543
Sa Liu,Min Jin,Yunhua Chen,Huichang Gao,Xuetao Shi,Wenhua Cheng,Li Ren,Yingjun Wang
Nanosized celluloses are attractive building blocks to generate hierarchically advanced materials and have been gradually explored in emulsion applications. Here we report a high internal phase emulsion (HIPE) prepared by using supramolecular cellulose nanocrystals (CNCs) as Pickering stabilisers via one-step emulsification, and interconnected macroporous hybrid hydrogels were produced by utilizing this HIPE as a template. A quadruple hydrogen bonding moiety 2-ureido-4[1H]-pyrimidone (UPy) was firstly grafted onto the surface of cellulose nanocrystals through simple free radical polymerization. The polymer grafting was confirmed by elemental analysis and thermogravimetry. The UPy modified CNCs (CNC-UPy) exhibited superior emulsion stabilising ability compared to the pristine CNCs, and the oil-in-water emulsions with an internal phase volume ratio of 80% showed good long-term stability. The properties of resulting macroporous polyHIPE hydrogels, such as swelling behaviours, porous structures and mechanical strength, were investigated on the dependence of CNC-UPy concentrations. In addition, the macroporous hybrid hydrogel exhibits excellent cytocompatibility and cell adhesion as demonstrated by mouse bone mesenchymal stem cell (mBMSC) culture. With these promising properties, the developed hydrogels demonstrate great potential as active biological scaffolds for tissue engineering.
研究领域:
材料科学
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生物医学工程
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组织工程
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产品关联
DOI
PubMed
高影响因子
ANALYST
·
2014
·Vol. 139
·期 21
·5482-5487
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IF 3.906
Hanping He,Xiaoqian Peng,Min Huang,Gang Chang,Xiuhua Zhang,Shengfu Wang
A small molecule modified sensor was developed for the detection of XGG trinucleotide repeats (X = C, T) by electrochemical impedance spectroscopy. The sensor (NCD/MPA/Au) was fabricated by immobilizing the nucleic acid recognition molecule (NCD) on the surface of a gold electrode through a condensation reaction between the amino-terminal end of the NCD linker and carboxylic groups in 3-mercaptopropionic acid that were self-assembled on the electrode surface. After the sensor was incubated with trinucleotide repeats, electrochemical impedance spectroscopy was performed using [Fe(CN)6]3−/4− as redox marker ions. XGG repeats (X = C, T) could be selectively detected based on the differences in charge transfer resistance (ΔRct) even in the presence of other trinucleotide repeats. The relationship between ΔRct and lg [concentration of CGG repeats] for the sensor was linear from 1 nM to 1 μM, enabling the quantification of the number of repeats. The electrochemical impedance sensor provides a simple and rapid method to detect trinucleotide repeats without requiring labelling and immobilizations of DNA, making it promising for the early diagnosis of neurodegenerative diseases; the sensor may be further extended to the detection of other special sequences of DNA.
研究领域:
生物医学工程
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分子生物学
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神经退行性疾病研究
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产品关联
DOI
PubMed
高影响因子
Catalysis Science & Technology
·
2017
·Vol. 7
·期 6
·1415-1422
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IF 5.773
Zheng Chen,Hongyan Pan,Qian Lin,Xin Zhang,Sheng Xiao,Shun He
Pd core–silica shell catalysts (PdX@SiO2, X = KBr, CTAB, SC) were prepared with and without the addition of three different functional molecules, potassium bromide (KBr), cetyltrimethyl ammonium bromide (CTAB) and sodium citrate dihydrate (SC), by the reverse micelle method, and then characterized by TEM, XRD, EDS, XPS and N2 adsorption/desorption. Results showed that the addition of functional molecules decreased the Pd particle size of the catalysts, and Br− of KBr and CTAB were selectively adsorbed on the surface of Pd particles. Among the prepared catalysts, the catalyst PdKBr@SiO2 had the highest H2O2 selectivity (98%) and productivity (716 mmol gPd−1 h−1). Compared with the catalyst Pd@SiO2 prepared without any additional functional molecules, whose H2O2 selectivity and productivity were 26% and 396 mmol gPd−1 h−1, respectively, the H2O2 selectivity and productivity of the catalyst PdKBr@SiO2 were 277% and 81% higher, respectively, than those of the catalyst Pd@SiO2. The reason for this was that the Br− of KBr can be easily selectively adsorbed on the coordinatively unsaturated sites of the Pd particle surface, which could decrease the energy of the Pd surface and restrain side reactions by inhibiting the O–O bond cleavage of O2 and H2O2. And the catalyst PdKBr@SiO2 has better stability and recyclability than the conventional supported catalyst Pd/SiO2. In contrast, the catalyst PdSC@SiO2 had the lowest H2O2 selectivity (23%) and productivity (395 mmol gPd−1 h−1) because the catalyst PdSC@SiO2 prepared with the functional molecule SC and without Br− had the smallest Pd particle size, which can lead to more coordinatively unsaturated sites and increase side reactions.
研究领域:
催化化学
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材料科学
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DOI
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 128
·106161-106170
·
IF 3.84
Li Gao,Jin Du,Chaozhan Wang,Yinmao Wei
Boronate adsorbents have been widely used in the extraction of cis-diol-containing molecules, but most do not have efficient capacity due to limited binding sites on their surface. In this work, a high binding capacity dendrimer-modified boronate affinity material (SiO2@dBA) was synthesized via introducing tris(2-aminoethyl)amine as branching points and using poly(amidoamine) as the main dendrimeric scaffold before modification by boronate groups. The high density of amino groups on the dendrimer supplied a large number of binding sites for modifying boronate groups. Thus the adsorption capacity (676.8 μmol g−1 for catechol, 771.3 μmol g−1 for dopamine, 770.0 μmol g−1 for adrenaline) of SiO2@dBA was greatly improved. Moreover, when coupled with large-volume injection and online column-switching solid phase extraction, SiO2@dBA was able to capture cis-diols from 10 000-fold interference and enrichment factors reached up to 497–514, which was 26- to 51-fold higher than those of analogous non-dendrimer materials. Especially, the proposed method exhibited a striking low limit of detection, 0.24 ng mL−1 for cytidine, 0.52 ng mL−1 for uridine, 0.37 ng mL−1 for guanosine, 0.67 ng mL−1 for adenosine. Finally, the method was successfully applied to online determination of trace nucleosides in healthy human urine. In conclusion, the prepared adsorbent has potential to effectively enrich a large range of trace cis-diol substances in real samples.
研究领域:
分析化学
·
生物医学
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产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2020
·
IF 8.758
Kaiming Zhang,Jiawen Sun,Jingyao Song,Chuanhui Gao,Zhe Wang,Chengxin Song,Yumin Wu,Yuetao Liu
Flexible conductive composites can be used as wearable strain sensors, which are widely used in the fields of new-generation robotics, electronic skin, and human detection. However, how to make conductive composites that simultaneously possess flexibility, stretchability, self-healing, and sensing capability is challenging research. In this work, we innovatively designed and prepared a silicone polymer conductive composite. MXenes and amino poly(dimethylsiloxane) were modified by small biomolecules via an esterification reaction and a Schiff base reaction, respectively. The modified MXenes are uniformly dispersed, which endows the composite with good electrical conductivity. The reversibility of multiple hydrogen bonds and imine bonds in the composite system makes it have ideal tensile properties and high-efficiency self-healing ability without external stimulation. The conductive composite containing 10 wt % A-MXenes showed an elongation of 81%, and its mechanical strength could reach 1.81 MPa. After repair, the tensile properties and the electrical conductivity could be restored to 98.4 and 97.6%, respectively. In addition, the conductive composite is further evaluated for the value of wearable strain sensors. Even after cut-healed processes, the conductive composite can still accurately detect tiny human movements (including speaking, swallowing, and pressing). This kind of self-healing MXene/PDMS elastomers based on the modification of small biomolecules has great potential as wearable strain sensors. This simple preparation method provides guidance for future multifunctional flexible electronic materials.
研究领域:
材料科学
·
纳米技术
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可穿戴技术
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电子皮肤
·
机器人技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）