Life-Basel
·
2023
·Vol. 13
·期 8
·1758
·
IF 3.2
Wenwen Song,Mingming Dai,Qianqian Shi,Chen Liang,Fangmeng Duan,Honghai Zhao
The potato rot nematode (Ditylenchus destructor) is one of the most destructive pests in the production of tuber crops, resulting in severely decreased yields and inferior product quality. In 2021, a great number of nematodes were detected in the roots ofMazus japonicus, a weed that is harmful to crop growth, in Qingdao, Shandong Province, China. The present study was undertaken to characterize and identify the nematodes isolated fromM. japonicusthrough morphological identification and molecular approaches. Their morphological characteristics were highly consistent with the descriptions ofD. destructorThorne, 1945. The nematodes collected fromM. japonicuswere identified asD. destructorhaplotype B using D1/D2 and sequence characterized amplified region (SCAR) primers. PCR-ITS-RFLP analysis was conducted to monitor intraspecific variations. In addition, the phylogenetic analysis of the internal transcribed spacer (ITS) demonstrated that thisD. destructorpopulation was clustered in haplotype B, supported by a 100% bootstrap value. Another assay, in whichM. japonicuswas inoculated with a mixture of the life stages ofD. destructor, was performed. This assay showed thatM. japonicusexhibited a high susceptibility toD. destructorin pots. This is the first record ofD. destructorparasitizingM. japonicusin China, and it is of great importance becauseM. japonicuscould be a potential reservoir forD. destructorin the field.Keywords:Ditylenchus destructor;Mazus japonicus;morphological characterization;molecular analysis
研究领域:
植物病理学
·
线虫学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2021
·
IF 3.205
Xiao-Pan Liu,Wen-Qian Sun,Tong-Xin Liu,Bing-Bing Liu,Chang-Po Chen
Due to the strong background interferences in the low-mass region and poor reproducibility of conventional organic matrices, it is of great importance to develop a novel matrix for matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) to qualitatively and quantitatively analyze small molecules. In this work, water-soluble fullerenol C 60 (OH) 24–26 was selected as a MALDI matrix for the analysis of low-molecular-weight compounds in consideration of optical absorption property, water solubility and stability. Compared with the traditional matrices, fullerenol demonstrated lower background interference and stronger peak intensity. In addition, the hydrophilic fullerenol could avoid the heterogeneous crystallization in sample preparation, increase the reproducibility and sensitivity of MALDI-MS, and ameliorate quantitative analysis of small molecules. With saccharin as model analyte, quantitative analysis was carried out using fullerenol as matrix. The results demonstrated satisfying reproducibility and good tolerance to salt. The limit-of-detection of the quantitative analysis was as low as 4 pmol, and the linear range is 1–100 μg mL −1 with R 2 greater than 0.99. The analytical results also showed excellent precision and accuracy, low matrix effect and good recovery rate. Fullerenol as a potential matrix was further validated in the quantification of saccharin sodium in different real food samples, such as nuts and drinks. This work not only confirms the potential of fullerenol for the quantitative analysis in food field, but also provides a new technique for rapid analysis of small molecules.
研究领域:
分析化学
·
食品科学
·
质谱技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Zhenzhao Wang,Lili Liu,Zhen Li,Linna Li,Xingtong Liu,Rikun Cui,Fujun Yao,Lei Tian,Xiaofeng Kang,YanLi Guo
Intercalated binding of anthracycline anticancer drugs to DNA is associated with anticancer mechanisms. The i-motif, a cytosine-rich DNA secondary structure found in the promoter region of oncogenes, is a primary target for anticancer drugs action. Therefore, it is significant to study the interaction between anthracycline drugs and i-motif for cancer therapy using simple, rapid and low-cost analytical methods. Here, we utilized α-HL protein nanopore, which offers high sensitivity and high signal-to-noise ratio to investigate the conformational transition of human telomere i-motif induced by various anthracycline drugs at the single-molecule level. Our results revealed for the first time that anthracyclines drugs could induced the unfolding of i-motif into hairpin DNA. Additionally, our experiment captured the current signal of the intermediate state during the unfolding of i-motif into hairpin DNA, a signal difficult to obtain by conventional analytical tools such as circular dichroism (CD) and UV–visible spectroscopy (UV–vis). This research provides a novel tool for investigating the interaction between small molecule and DNA and lays the foundation for future screening of drugs acting on i-motif targets, as well as analyzing their therapeutic mechanism.
研究领域:
生物化学
·
分子生物学
·
药理学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2019
·
IF 2.813
Hao Zhang,Dong-Dong Zhou,Feng-Qing Yang,Zheng-Ming Qian,Chun-Hong Li,Wen-Jia Li,Sheng-Peng Wang,Yi-Tao Wang
Plant polyphenols can form functional coatings on various materials through self-polymerization. In this paper, a series of modified capillary columns, which possess diversity of charge characteristics for modulating electroosmotic flow (EOF), were prepared by one-step co-deposition of gallic acid (GA), a plant-derived polyphenol monomer , and branched polyethyleneimine (PEI). The physicochemical properties of the prepared columns were characterized by Fourier transform infrared spectroscopy (FT-IR), UV–Vis spectroscopy and scanning electron microscopy (SEM). The magnitude and direction of EOF of GA/PEI co-deposited columns were modulated by changing a series of coating parameters, such as post-incubation of FeCl 3 , co-deposition time, and deposited amounts of GA and PEI with different relative molecular mass (PEI-600, PEI-1800, PEI-10000, and PEI-70000). Furthermore, the separation efficiencies of the prepared GA/PEI co-deposited columns were evaluated by separations of small molecules , including organic acids, polar nucleotides, phenols, nucleic acid bases and nucleosides . Results indicated that modulating of EOF plays an important role in enhancing the separation performance and reversing the elution order of the analytes. Finally, the developed method was successfully applied to quantitative analysis of acidic compounds in four real samples. The recoveries were in the range of 73.5%–85.8% for citric acid , benzoic acid , sorbic acid , salicylic acid and ascorbic acid in beverage and fruit samples, 101.6%–104.9% for cinnamic acid , vanillic acid , and ferulic acid in Angelica sinensis sample, while 84.6%–97.8% for guanosine-5′-monophosphate, uridine-5′-monophosphate, cytosine-5′- monophosphate and adenosine-5′-monophosphate in Cordyceps samples. These results indicated that the co-deposition of plant polyphenol-inspired GA/PEI coatings can provide new opportunities for EOF modulation of capillary electrophoresis .
研究领域:
分析化学
·
植物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Omega
·
2020
·
IF 2.87
Yumeng Yang,Dan Gao,Rui Qian,Yuyang Jiang
Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) using conventional organic matrices for detection of small molecules has some limitations, such as heterogeneous analyte/matrix co-crystals, as well as interference of matrices in the low-molecular-weight range. In this work, a zeolite framework nanomaterial, TS-1, was applied as a MALDI matrix for the analysis of small molecules by MALDI-MS for the first time. To improve the signal intensity and reproducibility, TS-1 was modified with polydopamine (TS-1@PDA). Using TS-1@PDA as a matrix, organic substances in the low-molecular-weight region such as amino acids, nucleosides, peptides, oligosaccharides, and fatty acids can be detected by MALDI-MS in positive ion mode. Compared with traditional organic matrices like 2,5-dihydroxybenzoic acid (2,5-DHB) and α-cyano-4-hydroxycinnamic acid (CHCA), TS-1@PDA has the advantages including the formation of uniform sample spots, small background interference at low molecular weight, and better salt tolerance. Furthermore, this matrix was employed for the analysis of endogenous glucose in urine samples, and the level of glucose was quantified with a linear range of 0–10 mM (R2 > 0.98). The results demonstrated that TS-1@PDA has the potential to be used as an effective MALDI matrix for the analysis of small molecules in biological samples with excellent reproducibility and moderate sensitivity.
研究领域:
分析化学
·
生物医学
·
质谱技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS RESEARCH
·
2014
·Vol. 29
·期 5
·633-640
·
IF 1.815
Jun Yao,Mei Yang,Yixiang Duan
Highly luminescent CdTe quantum dots (QDs) were prepared through a fast, facile, and straightforward method. The crystal structure, particle size, optical properties as well as molecular interactions between the CdTe QDs and their capping agents have been investigated by high resolution transmission electron microscopy, selected area electron diffraction, scanning transmission electron microscope–energy dispersive x-ray spectroscopy, UV-vis absorption, photoluminescence, and Fourier transform infrared, respectively. The results illustrate that the CdTe nanoparticles exhibit cubic structure and the average crystallite size is 2.3 nm. Meanwhile, fluorescence and UV-vis spectroscopic techniques were used to study the interaction between hemin and the well-defined CdTe QDs. In weak basic media, the fluorescence of CdTe QDs was quenched notably by hemin, and the quenching values were proportional to the concentration of the quencher in a certain range. The quenching mechanism was discussed to be a dynamic quenching procedure, collisional process, and hemin as a fluorescence quencher donated its electron to CdTe QDs to occupy the hole and accordingly disrupted the electron–hole recombination.
研究领域:
纳米材料科学
·
生物分析化学
·
光学
✓ 已验证
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2025
·
IF 6
Lin Cheng,Yitong Li,Weiwei Ji,Jing Yan
Rapid and sensitive protein detection methods are of benefit to clinical diagnosis, pathological mechanism research, and infection prevention. However, routine protein detection technologies, such as enzyme-linked immunosorbent assay and western blot, suffer from low sensitivity, poor quantification and labourious operation. Herein, we developed a fully automated protein analysis system to conduct fast protein quantification at the single molecular level. It streamlines antibody-coated beads-based protein capture, fluorescent labelling with fluorophore-conjugated antibodies, and flow-cytometric optical detection in a microfluidic chip, achieving sample-in quantitative result-out protein detection within 60 min. This platform employs a combination of spiral and ultrasonic mixing, and realizes highly efficient protein capture and immunolabelling. Leveraging non-Newtonian fluids, the beads can be well-aligned at the focus of the optical module, yielding robust protein quantification results with a low coefficient of variation of 0.4%. As a proof of concept, interleukin 6 was determined using the integrated instrument. The results showed good linear correlation (R 2 =0.9938) with that obtained from Roche Electrochemiluminescence Immunoassay Analyzer (ECLIA). Moreover, the single molecule immunoanalyzer achieved a lower limit of detection (LOD) of 16 fg/mL and exhibited an 8-fold increase in sensitivity compared to ECLIA. With short turnaround time and less human intervention, the developed protein quantification system could become a valuable tool for detection of disease markers and precision medicine.
研究领域:
生物医学工程
·
临床诊断
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHROMATOGRAPHIA
·
2020
·Vol. 83
·期 8
·985-992
·
IF 1.596
Zhao Yang,Zeng Jiao,You Junheng,Duan Yuanxing,Li Yuandong,Liu Chunbo,Liu Zhihua,Yang Liu,Shen Qinpeng,Li Zhenjie
A poly ( N -isopropyl acrylamide) (polyNIPA)-based hybrid column for capillary liquid chromatography (cLC) was prepared with N -isopropyl acrylamide (NIPA) and alkoxysilanes through a sol–gel “one-pot” method. In this process, the hydrolyzed tetramethoxysilane (TMOS) and vinyltrimethoxysilane (VTMS) were the precursors for the synthesis of a silica-based monolith and subsequently reacted with organic monomer (NIPA) via free radical polymerization within the confines of a capillary. The effects of polymerization temperature, the volume of TMOS, VTMS, and acetic acid (HAc) as well as the amount of progens [polyethylene glycol 10,000 (PEG10000) and urea] on the morphology and permeability were investigated in detail. Uniform pore structure and good mechanical stability were obtained for columns prepared under optimized conditions. Separation of various neutral, acidic and basic analytes was achieved by cLC in reversed-phase liquid chromatography (RP-LC) mode. Moreover, the resulting hybrid column was also applied for the analysis of small organic pollutants, including polycyclic aromatic hydrocarbons (PAHs), perfluorinated compounds (PFCs) and chlorophenols (CPs). The successful application demonstrated its potential in the separation of small molecules.
研究领域:
分析化学
·
材料科学
✓ 已验证
DOI
高影响因子
Small
·
2010
·Vol. 7
·期 1
·87-94
·
IF 6.171
Yi-Lun Ying,Hai-Yan Wang,Todd C. Sutherland,Yi-Tao Long
An aptamer is a specific oligonucleotide sequence that spontaneously forms a secondary structure capable of selectively binding an analyte. An aptamer’s conformation is the key to specific binding of a target molecule, even in the case of very closely related targets. Nanopores are a sensitive tool for the single-molecule analysis of DNA, peptides, and proteins transporting through the pore. Herein, a single α -hemolysin natural nanopore is utilized to sense the conformational changes of an adenosine 5’-triphosphate (ATP)-binding aptamer (ABA). The known DNA sequence of the ABA is used as a model to develop real-time monitoring of molecular conformational changes that occur by binding targets. The native, folded ABA structure has a nanopore unfolding time of 4.17 ms, compared with 0.29 ms for the ABA:ATP complex. A complementary 14-mer strand, which binds the ABA sequence in the key nucleic acids responsible for folding, forms linear duplex DNA, resulting in a nanopore transit time of 0.50 ms and a higher capture probability than that of the folded ABA oligomer. Competition assays between the ABA:ATP and ABA:reporter complexes are carried out, and the results suggest that the ABA:ATP complex is formed preferentially. The nanopore allows for the detection of an ABA in its folded, ATP-bound, and linear conformations.
研究领域:
分子生物学
·
生物传感器
·
核酸化学
·
单分子检测
·
转化医学
✓ 已验证
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2016
·
IF 7.145
Lianfang Chen,Junjie Ou,Hongwei Wang,Zhongshan Liu,Mingliang Ye,Hanfa Zou
Although thousands of metal–organic frameworks (MOFs) have been fabricated and widely applied in gas storage/separations, adsorption, catalysis, and so on, few kinds of MOFs have been used as adsorption materials while simultaneously serving as matrixes to analyze small molecules for laser desorption/ionization mass spectrometry (LDI-MS). Herein, a new concept is introduced to design and synthesize MOFs as both adsorption materials and matrixes according to the structure of ligands and common matrixes. The proof of concept design was demonstrated by selection of 2,5-pyridinedicarboxylic acid (PDC) and 2,5-dihydroxyterephthalic acid (DHT) as ligands for synthesis of MOFs. Two Zr(IV)-based MOFs of UiO-66-PDC and UiO-66-(OH)2 were synthesized and applied for the first time as new matrixes for analysis of small molecules by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Both of them showed low matrix interferences, high ionization efficiency, and good reproducibility when used as matrixes. A variety of small molecules, including saccharides, amino acids, nucleosides, peptides, alkaline drugs, and natural products, were analyzed. In addition, UiO-66-(OH)2 exhibited potential for application in the quantitative determination of glucose and pyridoxal 5′-phosphate. Furthermore, thanks to its intrinsically large surface area and highly ordered pores, UiO-66-(OH)2 also showed sensitive and specific enrichment of phosphopeptides prior to MS analysis. These results demonstrated that this strategy can be used to efficiently screen tailor-made MOFs as matrixes to analyze small molecules by MALDI-TOF-MS.
研究领域:
分析化学
·
质谱技术
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2025
·
IF 2.8
Xinyuan Ye,Lili Jiang,Ying Xiong,Cui Zhang,Chenxi Wang,Meiling Qi,Chenyue Zhu,Yi Chen,Zhifeng Du,Zhongzhe Cheng,Hongliang Jiang
Oligonucleotide (ON) is one of the rapidly developing fields in biotherapeutics. Ultra high-performance liquid chromatography high-resolution mass spectrometry (UHPLC-HRMS) has been widely used for the identification of metabolites due to its high sensitivity, high resolution, and ability to provide structural information. The identification of ON metabolites in matrix has been reported by UHPLC-HRMS, however, manual data processing is time-consuming. In this study, an analytical strategy based on UHPLC-QTOF-MS/MS and molecule profiler software was established and employed for the automatic identification of metabolites of ONs. Fomivirsen (FMVS), a 21-mer antisense oligonucleotide with 20 phosphorothioate linkages, was selected as proof of concept. Firstly, the sample preparation and UHPLC-QTOF-MS/MS condition were optimized. Secondly, the feasibility of the automatic identification of ON metabolites by this strategy was verified using enzymatic digests of FMVS. Finally, in vitro and in vivo metabolites of FMVS were identified. As a result, the enzymatic digests of FMVS were successfully identified by the established strategy, and a total of 17 metabolites were identified from serum, plasma and tissues. FMVS was mainly metabolized by 3′-exonuclease in plasma, liver and kidney. This is the first study on metabolites identification of FMVS, and the proposed strategy would simplify the identification of ON metabolites, thus could be used for other ON metabolites.
研究领域:
生物技术
·
药物代谢研究
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2024
·
IF 5.6
Qingshan Dai,Yanyan Zhu,Xianzhun Luo,Yongxin Li
Single-entity-based sensing platform has many advantages for real-time assays, such as single-molecule analysis, or targets detection in confined environment. In this contribution, a new single Au nanowire (NW) – Au@Pt/Au nanoparticles (Au@Pt/Au NPs) conjugated system was established and used for the detection of thrombin by using surface-enhanced Raman spectroscopy (SERS) technique. This method was mainly based on electrostatic attraction between the capture (thrombin aptamer) and probe molecules (crystal violet, CV) on the surface of Au NW - Au@Pt/Au NPs conjugation, reducing the adsorption of CV molecules on conjugation surface, and resulting the decrease of SERS signals. The addition of thrombin could bind with thrombin aptamer due to specific interaction, leading to the decrease of electrostatic effect from thrombin aptamer for CV diffusion to conjugation surface, and the SERS signals could be recovered. This thrombin sensing method showed high sensitivity and selectivity, and the linear range for thrombin assay was 0.01 nM–100 nM with a detection limit of 1.35 pM. This single Au NW - Au@Pt/Au NPs conjugation has more SERS hotspots and small overall size, which offers unparalleled advantages over other sensing system and can be applicable for real time analysis, especially at single molecule/cell level.
研究领域:
生物传感
·
纳米技术
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）