Horticulturae
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2022
·Vol. 8
·期 10
·891
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IF 2.923
Huanxin Zhang,Guoquan Li,Chengpu Yan,Xinlong Zhang,Na Cao,Meiwang Le,Xinlong Hu,Fanghong Zhu,Wenge Liu
Waterlogging is a serious abiotic stressor that drastically hinders the growth and productivity of melon (Cucumis melo) around the world, due to the reduction in available oxygen levels in the waterlogged tissues. However, the mechanism underlying the responses to waterlogging stress in melon is largely unknown. In this study, physiological and transcriptome data of the waterlogging-sensitive accession ‘L39’ and the waterlogging-tolerant accession ‘L45’ were investigated under conditions of normal water supply and waterlogging stress. The results showed that ‘L45’ exhibited higher chlorophyll contents and lower REL (relative electrolyte leakage) and MDA (malondialdehyde) contents compared with ‘L39’ under waterlogging stress. Additionally, waterlogging stress only led to the stomatal closure and chloroplast damage of ‘L39’. In total, 1748 genes were differentially expressed in the leaves of waterlogging-stressed ‘L45’ compared with control, whereas 3178 genes were differentially expressed in ‘L39’. Further analysis indicated that genes related to chlorophyll synthesis and photosynthesis were more depressed in ‘L39’, while sugar cleavage, glycolysis, and fermentation genes were highly induced in ‘L39’ compared with ‘L45’. The expression of genes involved in ROS (reactive oxygen species) scavenging and hormone signaling significantly differed between ‘L39’ and ‘L45’ in their response to waterlogging stress. Moreover, a total of 311 differentially expressed transcription factors were waterlogging-responsive, among which members of the ERF (ethylene response factor), bHLH (basic helix-loop-helix), and WRKY families might play crucial roles in waterlogging tolerance in melon. This study unraveled the molecular responses to waterlogging stress in melon and could provide helpful candidate genes for further molecular breeding of waterlogging-tolerant melon varieties.Keywords:Cucumis melo;waterlogging;transcriptome;molecular response
研究领域:
植物科学
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
ENVIRONMENTAL POLLUTION
·
2023
·
IF 8.9
Wenjing Zhang,Yue Han,Shuxin Li,Shengyu Wu,Juanfen Chai,Jincai Ma,Dayi Zhang,Yuesuo Yang
The diversity of colloidal types and the differences in the composite ratios in porous media are important factors governing the migration and biological risk of pathogenic microorganisms in the subsurface environment. In this study, E. coli O157:H7 was subjected to co-migration experiments with different compositions of the composite colloid montmorillonite (MMT)-Fe 2 O 3 , and the biomolecular response of E. coli under the action of colloids was analyzed by Raman spectroscopy to quantify the risk of E. coli under the action of composite colloids based on both. The results showed that Fe 2 O 3 colloids inhibited E. coli migration mainly by electrostatic adsorption and reduced E. coli metabolism. MMT colloid inhibited E. coli migration mainly by blockage, and E. coli metabolism increased, and surface macromolecules decreased to reduce E. coli adhesion. MMT-Fe 2 O 3 complex colloids inhibited migration through electrostatic attraction between the two and formation of cohesive colloids, with reduced E. coli metabolism and insignificant biomolecular response. It was briefly assessed that the composite colloids reduced E. coli risk less strongly than single colloids, stemming from the difference in the mechanism of influence and the actual need to consider colloid interactions. This conclusion can inform the management and control of pathogen risk in porous media environments.
研究领域:
环境科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Xin Li,Yanli Lu,Zhenghan Shi,Guang Liu,Gang Xu,Zijian An,Huan Xing,Qingmei Chen,Ray P.S. Han,Qingjun Liu
Two-dimensional (2D) materials provides large surface area for H 2 O molecules adsorption and interaction, which has been utilized in humidity sensing and noninvasive monitoring of physiological events such as respiration. However, disadvantages including slow response or modest response hinder the accurate tracking of human breath. Hence, MXene , the new member of 2D material family with excellent hydrophilicity and tunable conductivity, has drawn much attention. In this work, the enhanced response of Ti 3 C 2 T x MXene to H 2 O molecules was achieved by onion-inspired assembling of Ti 3 C 2 T x MXene and chitosan-quercetin hybrid layer-by-layer. The chitosan-quercetin modified multilayers suppress the degradation of MXene in environment, exhibiting superior response (317 % at 90 % RH), ultrafast response (0.75 s), and long-term stability (more than 15 days). Integrated with a wireless flexible detecting tag, the multilayers realized wearable human respiration monitoring with robust, accurate tracking capacity, offering a facile and feasible method for versatile physiological monitoring based on humidity sensing.
研究领域:
材料科学
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生物医学工程
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传感器技术
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产品关联
DOI
高影响因子
NANOTECHNOLOGY
·
2021
·Vol. 32
·期 37
·375601
·
IF 3.874
Hanbin Xu,Huan Liang,Jing Zheng,Guobao Ning,Li Wang,Jing Zeng,Hui Zhao,Can-Peng Li
Fingerprints formed by the raised papillary ridges are one of the most important markers for individual identification. However, the current visualization methods for latent fingerprints (LFPs) suffer from poor resolution, low contrast, and high toxicity. In this work, the CsPbBr3/Cs4PbBr6 nanostructured composite crystal (CsPbBr3/Cs4PbBr6 NCC) were synthesized via a simple chemical solvent-assisted method. Compared with conventional perovskites, the as-prepared CsPbBr3/Cs4PbBr6 NCC present an outstanding long-term environmental and water stability with 42% and 80% photoluminescence intensity remaining after 28 d under water and air conditions, respectively. Moreover, a special response to biomolecules from fingerprints was observed due to the hydrophobic interactions between the CsPbBr3/Cs4PbBr6 NCC surface hydrophobic ligands (oleyl amine and oleic acid) and the hydrophobic groups in the biomolecules from the human fingers. Clear LFPs images were visualized in a bright environment illuminating the prepared CsPbBr3/Cs4PbBr6 NCC powder under UV light of wavelength 365 nm. The images were also obtained on porous and non-porous surfaces such as metal, plastic, wood, glass, and paper products. These perovskite nanocrystals are expected a stable and bright luminescent labeling agent for LFPs visualization and have potential application in crime scene and personal identifications.
研究领域:
材料科学
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法医学
·
光学
✓ 已验证
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2025
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IF 6.7
Tingting Xiao,Weiqiang Wang,Lingyi Zhao,Jian Xie,Weiping Cai
Illicit drug abuse has brought big challenges to public health, because it has caused severe damage to the human central nervous system. The development of a rapid, quantified, and on-site detection of illicit drugs is of key importance for the control of drug abuse. However, it remains challenging due to biological sample interference and difficulties in achieving specific recognition of the drug molecule. Here, we report a molecule-responsive two-component surface-enhanced Raman scattering (SERS) platform for the capture and detection of illicit drugs. Under optimized conditions, the system demonstrates a linear response to cocaine concentrations ranging from 1 ppb to 10 ppm (R2 > 0.99), with an ultralow detection limit of 1.5 ppb. Successful application in spiked bovine serum validates the method’s robustness against complex biological matrices. This work establishes a novel paradigm for detecting trace-level illicit drugs with significant forensic potential. The design principle might be applied to other chemical detection.
研究领域:
分析化学
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生物传感
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法医学
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纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
·
2021
·
IF 4.291
Mengyang Lu,Qi Zhang,Zhicheng Zhang,Xueqi Wang,Bing Yan,Yongqian Wang
There are a variety of biological ion channels in organisms, which change under the influence of the environment and regulate the material transport at both ends of the biofilm. Inspired by this, a large number of studies on biomimetic nanochannels have been carried out. The lack of selective specificity and detection sensitivity are considered as main drawbacks in biomimetic nanochannels, and the combination of nanomaterials and biomimetic nanochannels is one of solutions. In this research, a confined self-assembly system composed of anodic aluminum oxide (AAO) templates and ZnS nanoparticles was fabricated by a negative pressure filtration method. Besides, L- Cysteine ( L- Cys) and D- Cysteine ( D- Cys) were modified into the confined self-assembly system by ultraviolet irradiation to enhance the sensitivity for biological molecules response. The confined self-assembly system showed specific selection of L- Cys and high sensitivity to biological molecules response. This work not only synthesized ZnS nanoparticles in biomimetic nanochannels controllably but also provided a new way of studying how to utilize nanomaterials to improve the response sensitivity of biomolecules in biomimetic nanochannels.
研究领域:
纳米技术
·
仿生学
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材料科学
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生物传感
✓ 已验证
DOI
高影响因子
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