Background Copper is a vital trace element that plays a crucial role in various physiological processes due to its ability to exist in multiple oxidation states. Inspired by natural enzymes, researchers have developed copper-based nanozymes that mimic enzyme functions, offering cost-effective and stable alternatives to traditional enzymes. Despite their promising properties, the design and synthesis of these nanozymes can be complex and challenging. To address this issue, this work explores an intuitive approach to construct “soft” nanozymes simply by binding Cu 2+ with its aptamers to form Cu(II)-aptamer complexes. Results Using a screening strategy, we identified a specific aptamer against Cu 2+ , designated P8, that significantly enhanced peroxidase-like activity when complexed with Cu 2+ , demonstrated by a notably smaller K m value. Furthermore, we investigated the effects of various lengths of a “molecular clamp” on the conformational dynamics of the P8 aptamer, which perturbs the encapsulation of Cu 2+ and modulated its peroxidase activity. Our findings indicate that optimizing the molecular clamp to a moderate length ( N d = 20) can further enhance the peroxidase activity of the Cu 2+ -P8 complex. The peroxidase-like soft nanozymes based on copper(II)-aptamer complexes were finally utilized for label-free detection of EDTA. The developed colorimetric sensing method exhibits outstanding detection capabilities for EDTA in Sprite and mineral water samples, achieving an extended dynamic range that accommodates higher concentrations through the incorporation of molecular clamps. Significance and novelty This work highlights a novel and straightforward approach to generate and improve the enzymatic properties of copper-based nanozymes. The strategy to regulate the enzymatic activity effectively addresses the limitations of narrow detection ranges commonly observed in traditional methods.
Ion migration, especially taking place at the interface of perovskite/charge transport layer, poses great threats to the stability of perovskite light–emitting diodes (PeLEDs). However, the existing strategies mitigating ion migration sacrifice efficient charge injection, thus limiting the PeLED performances. Herein, an interfacial layer of a biaxially charge-polarized molecule, bi-4-(N-carbazolyl)phenyl)phenylphosphine oxide (BCPO), is applied. The strong surface coordination of BCPO not only manifests a high-quality metal halide perovskites (MHPs) emitter with improved defect passivation and crystallinity but also establishes a rigorous vertical dipole alignment, inducing band-bending for favorable electron injection. Additionally, the in-plane geometry of the electron-withdrawing carbazolylphenyl units achieves intimate lateral electrostatic contact with the MHPs surface, realizing stronger chemical interaction that suppresses the escape of halide at the interface. Encouraged by these achievements, the optimized PeLEDs with BCPO demonstrate maximum external quantum efficiency of 25.8% and T 50-EL lifetime of 13.4 h, representing one of the best green PeLEDs reported. This work newly opens a synergistic strategy, combined with molecular structure design and interfacial engineering, for simultaneously realizing efficient and color-stable PeLEDs, which tackles the crucial challenges for their commercialization.
Teng Peicheng,Wang Zhao,Kang Yu,Zhang Yikai,Wang Shutao
Multilayer elastic printed circuit boards (E-PCBs) have emerged as advanced configurations for integrating electronic components on elastomer substrates. However, weak inter-layer adhesion in elastic substrates can result in deformation-induced cracks and delamination in real-world applications. In this study, we present a novel approach to address this challenge by developing supramolecular sandwiched elastomers with high-adhesion skin and mechanically strong cores. This is achieved by precisely regulating the coordination bonds between different metal ions and the ethylenediamine groups within the aminated polydimethylsiloxane. The resulting sandwiched elastomer exhibits strong adhesion between layers (adhesion strength of 0.80 MPa), breaking strength of 0.81 MPa, Young’s modulus of 0.49 MPa, and maximum strain exceeding 500%. Leveraging these properties, the multilayered configuration of E-PCBs can be efficiently and securely assembled using the designed sandwiched elastomers through a simple layer-mounting method. This study offers a promising avenue for the simple and effective fabrication of mechanically stable multilayer flexible devices, eliminating the need for additional adhesive layers and complex structural constructions.
危险品化学品经营许可证（带存储）