SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Sujing Wang,Xin Li,Jiaqing Xie,Bingying Jiang,Ruo Yuan,Yun Xiang
Hg 2+ ions are highly toxic and bioaccumulative pollutant that can lead to adverse toxicological effects on environment and human health, and the establishment of simple and sensitive methods for the detection of trace Hg 2+ ions is thus of great significance. On the basis of a new metal ion-triggered and DNA-fueled molecular machine strategy, we have established a sensitive and convenient approach for detecting Hg 2+ in different water samples without involving in any enzymes or nanomaterials for signal enhancement. The target Hg 2+ ions associate with the DNA complex probes through the formation of specific thymine-Hg 2+ -thymine (T-Hg 2+ -T) mismatched base pairing, which results in the release of the fluorescently quenched ssDNA from the complex probes to expose the pre-blocked toeholds. Subsequently, the fuel DNA further binds to the toeholds and triggers the operation of the molecular machine via strand displacement reactions to recycle the target ions, leading to the release of many fluorescently quenched ssDNAs for amplified monitoring of Hg 2+ within the dynamic range of 0.1–100 nM with a detection limit of 65 pM. Besides, with specific T-Hg 2+ -T coordination, the established approach shows high selectivity to the target ions and can be employed to detect Hg 2+ in tap and river water samples.
研究领域:
生物化学
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环境科学
·
分析化学
✓ 已验证
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2021
·
IF 7.46
Yu Zhang,Haifeng Lu,Bing Yan
In this work, a boric acid covalently functionalized lanthanide MOF (Ln-MOF) sensor was constructed for the first time to monitor of urinary N-acetylneuraminic acid (NANA) to early diagnose of lung cancer, NANA can be used as biomarker of lung cancer. Considering that the specific binding affinity between boronic acid group and cis-diol can probably cause special influence towards the fluorescence of Ln-MOF, a boric-acid-functionalized Ln-MOF (B-EuMOF) sensor was first designed by covalent modification, which is proved to be an effective ratiometric fluorescent sensor of NANA, a urinary biomarker of lung cancer containing cis-diol. This designed NANA sensor shows special enrichment capacity, high selectivity and sensitivity, fast response, excellent reusability and more important, the ratiometric sensing result can be clearly distinguished by naked eye. Based on the luminescent sensing property of B-EuMOF towards NANA and the capture function of boric acid group, a molecular robot system which can monitor lung cancer at early stage is constructed. In this molecular robot system, boronic acid group serves as the actuator using ‘functional-hand’ to grasp NANA for the molecular robots. The fluorescence of B-EuMOF (I 470 nm /I 614 nm ) act as the sensor of the molecular robots. And the processor is constructed by combinational logic gates (INHIBIT and AND) which can control the molecular robots by logical operation of fluorescence response of B-EuMOF towards NANA. This work proposes first example of Ln-MOFs based molecular robot and what’s more, paves new way for the combination of fluorescent sensor and intelligent molecular devices.
研究领域:
医学诊断
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生物化学
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纳米材料科学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Materials Chemistry B
·
2019
·Vol. 7
·期 36
·5554-5562
·
IF 5.047
Jianing Zhang,Jing Jin,Yanqiu Du,Xiang Zhou,Haojun Liang,Wei Jiang
DNA-functionalized gold nanoparticles (DNA–AuNPs) have shown great potential and exciting opportunities for constructing machine-like nanodevices. Nonthiolated DNA can be grafted onto gold surfaces via DNA bases, such as polyadenine (polyA)–DNA. The colloidal stability of polyA–DNA–AuNPs has a significant dependency on salt and pH that affects the assembly of AuNPs and their application in polyA–DNA molecular machines. High salt and low pH value contribute to the stabilization of polyA–DNA–AuNPs. In acid conditions, adenine can be protonated and becomes positively-charged, thus enhancing the adsorption of polyA–DNA onto the gold surface by electrostatic interactions; coordination of multiple interactions achieves a high DNA grafting density and colloidal stability. In addition, the length of adenine has an important effect on the efficiency of the DNA machine, while the length of thymine has little effect when the thymine length is less than or equal to seven. The assembly of AuNPs driven by dynamic polyA–DNA molecular machines was successfully accomplished with A5-DNA and A9-DNA. A moderate concentration of catalyst oligomer (50 nM) could improve the DNA hybridization efficiency. The A9-DNA based molecular machine is more efficient than the A5-DNA based one because of the larger amount of A9-DNA on the AuNPs, which increases the probability of collisions between complementary DNA strands. Therefore, polyA–DNA functionalized nanoparticles can be used as a basic unit to construct assembly-ordering structures and achieve dynamic molecular machines to be applied in the molecular diagnostics field.
研究领域:
纳米技术
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生物化学
·
分子诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nature Communications
·
2018
·Vol. 9
·期 1
·1-7
·
IF 12.353
Ge Xiaolin,He Yubin,Liang Xian,Wu Liang,Zhu Yuan,Yang Zhengjin,Hu Min,Xu Tongwen
Synthetic polyelectrolytes, capable of fast transporting protons, represent a challenging target for membrane engineering in so many fields, for example, fuel cells, redox flow batteries, etc. Inspired by the fast advance in molecular machines, here we report a rotaxane based polymer entity assembled via host–guest interaction and prove that by exploiting the thermally triggered translational motion (although not in a controlled manner) of mechanically bonded rotaxane, exceptionally fast proton transfer can be fulfilled at an external thermal input. The relative motion of the sulfonated axle to the ring in rotaxane happens at ~60 °C in our cases and because of that a proton conductivity (indicating proton transfer rate) of 260.2 mS cm −1 , which is much higher than that in the state-of-the-art Nafion, is obtained at a relatively low ion-exchange capacity (representing the amount of proton transfer groups) of 0.73 mmol g −1 .
研究领域:
材料科学
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化学工程
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电化学
·
分子机器
✓ 已验证
产品关联
DOI
PubMed
高影响因子
POLYMER TESTING
·
2022
·
IF 4.931
Xiaotao Wang,Zhuofan Chen,Yebin Yang,Huiling Guo,Yingkui Yang,Chak-Yin Tang,Xuefeng Li,Wing-Cheung Law
It is difficult to form nanoparticles by traditional polymerization methods, using the azobenzene monomer with large hindrance. In this work, distillation precipitation polymerization (DPP) with lowering the monomer content at the beginning and distilling the solvent at a constant speed. Monofunctional 6-(4-methoxy-4′-oxygen-azobenzene) hexyl methacrylate (Azo) as a photo-responsive monomer and methacrylic acid (MAA) as a pH responsive monomer, were used to synthesize UCNPs@SiO 2 @PAzo/MAA nanoparticles. After the SiO 2 was etched, UCNPs@PAzo/MAA nanocapsules (NCs) were formed. The trans isomer in the PAzo shell is converted into cis under 980 nm near-infrared (NIR) irradiation, leading to a variation of size and hydrophilicity of the nanocapsules. Controlled drug loading (∼17.5%) could be performed using UV irradiation, in which the pendant Azo groups have an cis “open” state and larger hydrophilicity. Under weak acid and NIR irradiation, the pendant Azo group with constant isomerization is like a “molecular impeller” agitator and the cumulative release rate of the doxorubicin (DOX) reaches 67.88%. Furthermore, UCNPs@PAzo/MAA nanocapsules were applied to the intracellular environment, and the cytotoxicity was studied. The cell images and cytotoxicity studies showed that the drug could be efficiently delivered to the nucleus by the UCNPs@PAzo/MAA nanocapsules in a controlled manner.
研究领域:
材料科学
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药学
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纳米技术
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2023
·
IF 9.5
Xiao-Fang Hou,Xu-Man Chen,Hari Krishna Bisoyi,Qi Qi,Tianchi Xu,Dongzhong Chen,Quan Li
Developing an artificial dynamic nanoscale molecular machine that dissipatively self-assembles far from equilibrium is fundamentally important but is significantly challenging. Herein, we report dissipatively self-assembling light-activated convertible pseudorotaxanes (PRs) that show tunable fluorescence and enable deformable nano-assemblies. A pyridinium-conjugated sulfonato-merocyanine derivative (EPMEH) and cucurbit[8]uril (CB[8]) form the 2EPMEH ⊂ CB[8] [3]PR in a 2:1 stoichiometry, which phototransforms into a transient spiropyran containing 1:1 EPSP ⊂ CB[8] [2]PR when exposed to light. The transient [2]PR thermally relaxes (reversibly) to the [3]PR in the dark accompanied by periodic fluorescence changes that include near-infrared emission. Moreover, octahedral and spherical nanoparticles are formed through the dissipative self-assembly of the two PRs, and the Golgi apparatus is dynamically imaged using fluorescent dissipative nano-assemblies.
研究领域:
化学
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纳米技术
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分子机器
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材料科学
·
生物物理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）