INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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2023
·Vol. 24
·期 8
·7320
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IF 5.6
Meiyu Sun,Ningning Liu,Jiahui Miao,Yanan Zhang,Yuanpeng Hao,Jinzheng Zhang,Hui Li,Hongtong Bai,Lei Shi
Oregano is a medicinal and aromatic plant of value in the pharmaceutical, food, feed additive, and cosmetic industries. Oregano breeding is still in its infancy compared with traditional crops. In this study, we evaluated the phenotypes of 12 oregano genotypes and generated F1progenies by hybridization. The density of leaf glandular secretory trichomes and the essential oil yield in the 12 oregano genotypes varied from 97–1017 per cm2and 0.17–1.67%, respectively. These genotypes were divided into four terpene chemotypes: carvacrol-, thymol-, germacrene D/β-caryophyllene-, and linalool/β-ocimene-type. Based on phenotypic data and considering terpene chemotypes as the main breeding goal, six oregano hybrid combinations were performed. Simple sequence repeat (SSR) markers were developed based on unpublished whole-genome sequencing data ofOriganum vulgare, and 64 codominant SSR primers were screened on the parents of the six oregano combinations. These codominant primers were used to determine the authenticity of 40 F1lines, and 37 true hybrids were identified. These 37 F1lines were divided into six terpene chemotypes: sabinene-, β-ocimene-, γ-terpinene-, thymol-, carvacrol-, andp-cymene-type, four of which (sabinene-, β-ocimene-, γ-terpinene-, andp-cymene-type) were novel (i.e., different from the chemotypes of parents). The terpene contents of 18 of the 37 F1lines were higher than those of their parents. The above results lay a strong foundation for the creating of new germplasm resources, constructing of genetic linkage map, and mapping quantitative trait loci (QTLs) of key horticultural traits, and provide insights into the mechanism of terpenoid biosynthesis in oregano.Keywords:oregano;essential oil;terpene chemotypes;hybrid;F1lines;SSR molecular marker
研究领域:
植物学
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遗传学
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药用植物学
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DOI
PubMed
高影响因子
Agronomy-Basel
·
2025
·Vol. 15
·期 3
·508
·
IF 3.4
Jing Yang,Nanchen Lai,Yiqin Zheng,Ruifeng Ji,Ping Wang,Wei Dai,Gantao Cheng,Xin He
Nanhaia speciosais a valuable industrial crop known for its significant edible and medicinal properties, attributed to its abundance of secondary metabolites. This study aims to elucidate the genetic relationships amongN. speciosa, enhance species identification accuracy, and select genetically stable, high-quality germplasms ofN. speciosa. To achieve this, we conducted chloroplast gene amplification and sequencing, alongside an analysis of the agronomic traits of 19N. speciosagermplasms. Additionally, non-targeted metabolomic profiling using UPLC-Q-Orbitrap/MS and chemometric methods was employed to assess their metabolic diversity and genetic relationships. The results revealed notable differences in DNA barcodes and secondary structures across the germplasms, with theatpF-atpH sequence successfully distinguishing all 19 germplasms into distinct varieties. Multivariate statistical analyses, including principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA), and hierarchical clustering, identified 12 key marker metabolites that effectively differentiated the germplasms, consistent with the observed phenotypic diversity. A clustering analysis integrating genetic, phenotypic, and metabolomic data further validated the utility of DNA barcoding for species identification. The results of the comprehensive analysis showed that NDL-2 and NDL-18 exhibited relatively good edible and medicinal properties, while NDL-1 and NDL-19 exhibited relatively poor edible and medicinal properties. This study provides crucial insights for the breeding and genetic improvement ofN. speciosaand related species, offering a valuable reference for the identification, conservation, and utilization of germplasm resources within theN. speciosa.
研究领域:
植物学
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遗传学
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代谢组学
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产品关联
DOI
高影响因子
Agronomy-Basel
·
2024
·Vol. 14
·期 11
·2566
·
IF 3.3
Ao Li,Peiwen Zhu,Deyan Kong,Lei Wang,Anning Zhang,Yi Liu,Xinqiao Yu,Lijun Luo,Feiming Wang
Rice is a major global staple crop, but rising temperatures and freshwater shortages have made drought one of the most severe abiotic stresses affecting agriculture. Additionally, rice blast disease and brown planthopper infestations significantly impact yields. Therefore, developing water-saving, drought-resistant, high-yielding, and disease-resistant rice varieties is critical for sustainable rice production. The new water-saving and drought-resistant (WDR) rice ‘Huhan 1516’, bred using marker-assisted selection (MAS) and marker-assisted backcrossing (MABC) techniques, addresses these challenges. This variety is highly adaptable to drought-prone and water-scarce regions such as the Yangtze and Huai River basins. With its high yield, drought tolerance, and broad-spectrum resistance to rice blast (conferred by thePi2gene) and brown planthopper (BPH), ‘Huhan 1516’ is suitable for various farming systems and environments. Field trials show that this variety outperforms control varieties by 2.2% in yield and exhibits moderate resistance to both rice blast and brown planthopper. ‘Huhan 1516’ has been recognized as a new water-saving and drought-resistant rice variety by the state, and as a released cultivar, it has great potential for market promotion and application.
研究领域:
植物学
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遗传学
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农学
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DOI
高影响因子
ZOOLOGICAL RESEARCH
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2018
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IF 0
Chao-Hao Chen,Bi-Jun Li,Xiao-Hui Gu,Hao-Ran Lin,Jun-Hong Xia
Table 1 Summary information on sex reversal of the F1 generation Group DES (g)/feed (kg) Individual number ( n ) Female Male Intersex Female rate (%) Group1 1 330 330 0 0 100 Group2 0.5 390 371 6 13 95 Control No DES 636 190 446 0 30 Total N/A 1 356 891 452 13 N/A Open in a separate window N/A: Not available.
研究领域:
水产养殖学
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遗传学
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产品关联
DOI
PubMed
PeerJ
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2023
·
IF 2.7
Wanwei Hou,Xiaojuan Zhang,Yuling Liu,Yujiao Liu,Bai li Feng
Background Faba bean ( Vicia faba L) is one of the most important legumes in the world. However, there is relatively little genomic information available for this species owing to its large genome. The lack of data impedes the discovery of molecular markers and subsequent genetic research in faba bean. The objective of this study was to analyze the faba bean transcriptome, and to develop simple sequence repeat (SSR) markers to determine the genetic diversity of 226 faba bean varieties derived from different regions in China. Methods Faba bean varieties with different phenotype were used in transcriptome analysis. The functions of the unigenes were analyzed using various database. SSR markers were developed and the polymorphic markers were selected to conduct genetic diversity analysis. Results A total of 92.43 Gb of sequencing data was obtained in this study, and 133,487 unigene sequences with a total length of 178,152,541 bp were assembled. A total of 5,200 SSR markers were developed on the basis of RNA-Seq analysis. Then, 200 SSR markers were used to evaluate polymorphisms. In total, 103 (51.5%) SSR markers showed significant and repeatable bands between different faba bean varieties. Clustering analysis revealed that 226 faba bean materials were divided into five groups. Genetic diversity analysis revealed that the relationship between different faba beans in China was related, especially in the same region. These results provided a valuable data resource for annotating genes to different categories and developing SSR markers.
研究领域:
植物学
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遗传学
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分子生物学
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DOI
PubMed
高影响因子
BIOCHEMICAL SYSTEMATICS AND ECOLOGY
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2013
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IF 1.153
Miao He,Zhezhi Wang
The genetic diversity among samples of Hypericum perforatum L. collected from the Qinling Mountains in China were studied using SRAP molecular markers and 12 SRAP primer combinations. A total of 183 bands and 153 polymorphic bands were obtained that accounted for 83.6% of the polymorphism. Analysis by the UPGMA method grouped the samples into three main clusters, with coefficients of similarity ranging from 0.57 to 0.97. Morphological traits were correlated with four of the contents of phenolic compounds , chlorogenic acid , rutin , hyperoside and quercitroside. Correlation analysis revealed that the hyperoside concentration was significantly and positively correlated with the density of black glands found along the vertical edges of the stem. The information generated by the SRAP markers was closely associated with some morphological variability. However, there was only a partial correlation between the chemistry and genetic data.
研究领域:
植物学
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遗传学
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分子生物学
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产品关联
DOI
高影响因子
Plants-Basel
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2023
·Vol. 12
·期 21
·3719
·
IF 4.5
Xiaogang Lei,Haoyu Li,Pingping Li,Huan Zhang,Zhaolan Han,Bin Yang,Yu Duan,Ndombi Salome Njeri,Daqiang Yang,Junhua Zheng,Yuanchun Ma,Xujun Zhu,Wanping Fang
The elite germplasm resources are key to the beautiful appearance and pleasant flavor of Biluochun tea. We collected and measured the agronomic traits of 95 tea plants to reveal the trait diversity and breeding value of Biluochun tea plant populations. The results revealed that the agronomic traits of Biluochun tea plant populations were diverse and had high breeding value. Additionally, we resequenced these tea plant populations to reveal genetic diversity, population structure, and selection pressure. The Biluochun tea plant populations contained two groups and were least affected by natural selection based on the results of population structure and selection pressure. More importantly, four non-synonymous single nucleotide polymorphisms (nsSNPs) and candidate genes associated with (−)-gallocatechin gallate (GCG), (−)-gallocatechin (GC), and caffeine (CAF) were detected using at least two GWAS models. The results will promote the development and application of molecular markers and the utilization of elite germplasm from Biluochun populations.Keywords:Biluochun tea plant;genome-wide association study;agronomic traits;single nucleotide polymorphisms;marker-assisted breeding
研究领域:
植物学
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遗传学
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植物育种
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产品关联
DOI
PubMed
高影响因子
ACS Omega
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2021
·
IF 3.512
Zili Song,Ruilin Zhao,Hongjiao Zhang,Penglin Wei,Landa Qi,Guocan Chen,Wen-Bing Yin,Wei Li
Edible mushrooms are important nutraceutical sources of foods and drugs, which can produce various nutritional ingredients including all essential amino acids. The method of rapid screening for the strains producing specific functional components is very indispensable. Homocitrate synthase is one of the key enzymes in the α-aminoadipate pathway for lysine biosynthesis and has preferable sequence conservation in Agaricales. Based on the blast of homocitrate synthase homologous genes of strains of Agaricales, we achieved combinations of degenerate primers as molecular markers to rapidly screen the lysine-producing edible mushrooms. The experimental results revealed that the consistency between PCR amplification and HPLC analysis attained 82 and 75% in strains of Agaricales and Polyporales, respectively. The finding showed that the molecular marker has higher universality for screening edible mushroom resources of Agaricales. This PCR-based approach shows excellent potential in evaluating and discriminating edible wild-grown mushrooms with high lysine content in Agaricales.
研究领域:
真菌学
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分子生物学
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食品科学
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产品关联
DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2023
·
IF 6.8
Huanxin Zhang,Panpan Si,Qiang Kong,Jinyue Ma
Naphthenic acids (NAs) are typical contaminants in heavily crude oil. Benzo[ a ]pyrene (B[ a ]P) is also a component of crude oil, but their combined effects have not been systematically explored. In this study, zebrafish ( Danio rerio ) were used as the test organisms, and behavioral indicators and enzyme activities were used as toxicity indicators. Combined with the effects of environmental concentrations, the toxic effects of low concentrations of commercially available NAs (0.5 mg/LNA) and benzo[ a ]pyrene (0.8 μg/LBaP) at single and compound exposures (0.5 mg/LNA and 0.8 μg/LBaP) were assayed in zebrafish, and transcriptome sequencing technology was used to explore the molecular mechanism of the two compounds affecting zebrafish from the molecular biology level. Sensitive molecular markers that could indicate the presence of contaminants were screened. The results showed that (1) zebrafish in the NA and BaP exposure groups exhibited increased locomotor behavior, and the mixed exposure group exhibited inhibition of locomotor behavior. Oxidative stress biomarkers showed increased activity under single exposure and decreased activity under the mixed exposure. (2) NA stress led to changes in the activity of transporters and the intensity of energy metabolism; BaP directly stimulates the pathway of actin production. When the two compounds are combined, the excitability of neurons in the central nervous system is decreased, and the actin-related genes are down-regulated. (3) After BaP and Mix treatments, genes were enriched in the cytokine-receptor interaction and actin signal pathway, while NA increased the toxic effect on the mixed treatment group. In general, the interaction between NA and BaP has a synergistic effect on the transcription of zebrafish nerve and motor behavior-related genes, resulting in increased toxicity under combined exposure. The changes in expression of various zebrafish genes are manifested in the changes in the normal movement behavior of zebrafish and the intensification of oxidative stress in the apparent behavior and physiological indicators. Capsule abstract We investigated the toxicity and genetic alterations caused by NA, B[ a ]P, and their mixtures in zebrafish in an aquatic environment using transcriptome sequencing technology and comprehensive behavioral analysis. These changes involved energy metabolism, the generation of muscle cells, and the nervous system.
研究领域:
环境科学
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毒理学
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分子生物学
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基因组学
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水生生物学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）