NUCLEIC ACIDS RESEARCH
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2024
·Vol. 52
·期 20
·12191-12200
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IF 16.6
Lyu Wenping,Zhu Jianji,Huang XiaoQin,Chinappi Mauro,Garoli Denis,Gui Cenglin,Yang Tao,Wang Jiahai
Mismatched base pairs in DNA are the basis of single-nucleotide polymorphism, one of the major issues in genetic diseases. However, the changes of physical and chemical properties of DNA caused by single-site mismatches are often influenced by the sequence and the structural flexibility of the whole duplex, resulting in a challenge of direct detection of the types and location of mismatches sensitively. In this work, we proposed a synthetic ligand-enhanced protein nanopore analysis of GG mismatch on DNA fragment, inspired by in silico investigation of the specific binding of naphthyridine dimer (ND) on GG mismatch. We demonstrated that both the importing and unzipping processes of the ligand-bound DNA duplex can be efficiently slowed down in α-hemolysin nanopore. This ligand-binding induced slow-down effect of DNA in nanopore is also sensitive to the relative location of the mismatch on DNA duplex. Especially, the GG mismatch close to the end of a DNA fragment, which is hard to be detected by either routine nanopore analysis or tedious nanopore sequencing, can be well differentiated by our ND-enhanced nanopore experiment. These findings provide a promising strategy to localize and discriminate base mismatches in duplex form directly at the single-molecule level.
研究领域:
生物物理学
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遗传学
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纳米技术
✓ 已验证
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Leina Dou,Tong Bu,Wentao Zhang,Bingxin Zhao,Qingfeng Yang,Lunjie Huang,Sihang Li,Chengyuan Yang,Jianlong Wang,Daohong Zhang
The nanomaterials based lateral flow immunoassays (LFIAs) have been widely used for on-site detection of small molecules contaminants. Nevertheless, their practical applications are often constrained by the complicated materials synthesis and surface modification process of nanomaterials. Herein, inspired by chemical-staining, a crystal violet (CV) staining based LFIAs was innovatively developed for the first time to analyze small molecules contaminant with significantly simplified behaviour and surpassing repeatability. This nanomaterials-free technique overcomes the intrinsic limitations of nanomaterials, owing to the superior protein dye property of CV. To demonstrate the feasibility of the unique strategy, furazolidone (FZD) was used as a model analyte, results showed detection limits of as low as 7 and 1.53 ng mL −1 by visual observation and quantitative analysis, respectively. Finally, to validate its applicability in actual conditions, the platform is well applied in three kinds of food samples (shrimp, fish and pork). Furthermore, as the detection principle is based on the molecular recognition between antigen and antibody, our method may find general applications by staining other antibodies against other target analytes.
研究领域:
分析化学
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食品安全
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生物医学
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DOI
高影响因子
International Journal of Electrochemical Science
·
2023
·
IF 1.5
Shaohua Luo,Yuxia Liu
Poly(acid chrome blue K) modified glassy carbon electrode (GCE) was electropolymerized by cyclic voltammetric sweep in the potential range from –0.2 to 0.9 V. The characteristic of Poly(acid chrome blue K) film was studied by different methods such as electrochemical impedance spectroscopy (EIS) and cyclic voltammetry. This modified electrode showed excellent electrocatalytic response to vanillin with the increase of the electrochemical responses. Under the optimal conditions a good linear voltammetric response could be obtained over the range of 1.0×10 -7 ~ 7.0×10 -5 mol dm -3 and the detection limit was got as 3.2×10 -8 mol dm -3 (3σ). The proposed method was successfully applied to biscuits and dark-chocolate samples with satisfactory results.
研究领域:
分析化学
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电化学
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食品分析
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传感器
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分子检测
✓ 已验证
DOI
高影响因子
International Journal of Electrochemical Science
·
2023
·
IF 1.5
Lei Xu,Feng Guo,Yong You,Jie Hu,Yanni Miao,Zixue Wu,Li Wang
A simple electrochemical sensor for the determination of sulfites in rice wine samples based on carbon black paste electrode (CBE) coupled with first-order derivative square wave voltammetry (SWV) was developed. CBE prepared by packing the mixture of carbon black powder and liquid paraffin oil with a ratio of 2:1 (w/w) into a cavity without any additional modification was reported. The cyclic voltammetry and SWV showed that the oxidation current of sulfite at CBE increased significantly compared with bare glassy carbon electrode or graphite paste electrode. Through first-order derivative processing, peaks became narrower and sharper leading to the enhancement of sensitivity. External standard calibration curve was in the range of 0.008~1.0 mmol L -1 with a detection limit of 6.0 μmol L -1 . This method was applied to the determination of sulfites in the commercial available rice wine samples and the results were compared with a titration method.
研究领域:
分析化学
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电化学
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食品分析
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生物传感
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分子检测
✓ 已验证
DOI
高影响因子
Analytical Methods
·
2014
·Vol. 6
·期 21
·8627-8632
·
IF 1.938
Jinjiao Shi,Ligang Chen
In this study, we investigated the possibility of reverse micelles which were used for the determination of rhodamine B in lipsticks followed by high performance liquid chromatography (HPLC). The reverse micelle of surfactant sodium 1,4-bis(2-ethylhexoxy)-1,4-dioxobutane-2-sulfonate (AOT) was formed in n-hexane. First, the samples were preprocessed by reverse micelle extraction, and then rhodamine B was extracted from the mixture with methanol by back-extraction. The effects of different parameters including the concentration of surfactant, pH, amount of potassium chloride, ultrasonic time and type of organic solvents were evaluated and optimized during reverse micelle formation. The limit of detection of rhodamine B was 0.01 μg g−1. The intra-day and inter-day precisions expressed as relative standard deviations were 3.6% and 4.5%, respectively. The recovery of rhodamine B obtained was 95.0%. The reverse micelle method proposed for the determination of rhodamine B in real samples of lipsticks has the advantages of simplicity, speed, high precision, low detection limit and the use of inexpensive equipment.
研究领域:
分析化学
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食品与化妆品安全
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药物分析
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分子检测
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环境与健康
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）