JOURNAL OF MOLECULAR LIQUIDS
·
2025
·
IF 5.2
Qiao Li,Shou-Jun Lai,Tingting Deng,Siyu Zhang,Teng Niu,Hai-xiong Shi,Hongmei Mu
A novel Schiff-base fluorescent probe (SA) has here been designed for lysine detection. A condensation reaction between thiophene-2-carboxaldehyde and 1,4-succinohydrazide was then used. The SA showed a selective “turn-on” fluorescence response (425 nm) to lysine in a pH-stable dimethyl sulfoxide (DMSO)/H₂O system (3:2, pH = 7.2), thereby achieving a detection limit of 8.21 × 10 −8 M. Both experimental and computational studies revealed that the SA formed a flexible V-shaped cavity via intramolecular non-covalent interactions. An adaptive encapsulation of lysine through synergistic O-H···N/O-H···O hydrogen bonds and σ-π interactions could, thereby, form a stable 1:1 complex. The present study has developed a rational design strategy for dynamic molecular probes and advanced high-performance sensing platforms for biomolecules.
研究领域:
分析化学
·
生物传感
·
有机化学
·
生物医学工程
✓ 已验证
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2022
·
IF 12.545
Qing Yu Liu,Zhen Qi Bu,Min Xia Quan,Ying Wu,Xuezhi Ding,Li Qiu Xia,Jiao Yang Lu,Wei Tao Huang
Inspired by information processing and communication in nature based on molecular recognition and structural diversity, ongoing efforts aim to development of artificial molecular or nano-systems for sensing, logic computing, and even data storage and safety. However, due to their preparation/functionalization shortcomings (laborious and time-consuming), poor flexibility and compatibility, and limited paradigm, it is still a big challenge whether simple molecules can be used to achieve comprehensive and universal applications from sensing to information storage and protection. Herein, we for the first time demonstrated a molecular paradigm—computer-like “basic input output system (BIOS)” which can realize “plug-and-play” sensing, information encoding, molecular cryptography, and steganography based on a simple artificial molecule (p-nitrophenol, PNP). Based on its molecular recognition and inherent chemical identities, PNP was utilized for colorimetric detection of multiple metal ions (Hg 2+ , Fe 3+ , Al 3+ , Cr 3+ ) and distinguishing their valence (like Cr 3+ /Cr 6+ , Fe 3+ /Fe 2+ ). Interestingly, PNP can achieve the “plug-and-play” fluorescent expansion of detection channels by directly mixing with fluorescent molecules, indicating that PNP molecule can be served as a molecular BIOS with flexibility and compatibility. Impressively, the selectivity embedded in PNP-based BIOS sensing system can be developed as molecular-level double cryptographic steganography to encode, encrypt and hide specific information (like the content of classical literature). This research not only provides a basic idea for building a molecular paradigm with “plug-and-play” flexibility and compatibility, but also provides ideas for the use of molecular sensing and informatization to open up the digitalization of the molecular world.
研究领域:
化学
·
信息科学
·
分子生物学
✓ 已验证
DOI
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2025
·
IF 3.1
Zeling Guo,Yu Meng,Xinyu Li,Jiangting Li,Shang Zhou,Rongrong Feng,Weiting Wu,Mingjing Xu,Jinhao Liu,Xiangfu Zeng,Weidong Zhao,Haijian Zhong
Hepatocellular carcinoma (HCC) is a serious concern worldwide. The published reports showed that aberrant CD95 receptor expression plays a critical role in apoptosis in liver cancer. While curcumin has shown promise in inducing apoptosis in liver cancer cells, its direct effects on CD95 expression during this process have not been thoroughly investigated. This study aims to quantitatively assess the expression of the CD95 receptor in HepG2 cells treated with different concentrations of curcumin using techniques such as fluorescence staining, single-molecule force spectroscopy (SMFS), and single-molecule recognition imaging (SMRI). Fluorescence staining results indicate a significant increase in CD95 expression following curcumin treatment. For the first time, SMFS and SMRI techniques were used to directly reveal the binding sites of CD95 on the cell membrane, with the number of binding sites increasing as the curcumin concentration increased. Additionally, the binding force between an antibody-modified probe and CD95 was strengthened with curcumin treatment, suggesting that curcumin enhances both the quantity and affinity of CD95 binding sites. This study provides new insights into curcumin-induced CD95-mediated apoptosis in liver cancer cells and highlights the potential of AFM techniques for investigating drug mechanisms. Overall, these findings may inform innovative therapeutic strategies for liver cancer and improve drug design processes.
研究领域:
肿瘤学
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分子生物学
·
药理学
✓ 已验证
DOI
PubMed
高影响因子
Journal of Physical Chemistry C
·
2017
·
IF 4.536
Qing Zheng,Ximing Huang,Yao Liu,Xiaotong Fang,Jie Zhang,Huibo Shao
Quantification of the amount of intermolecular hydrogen bonding between ferrocenemethanol and 3-mercaptopropanoic acid on gold was performed by cyclic voltammetry as well as by the finite element simulation, displaying a proof-of-concept that the slight changes of cyclic voltammetry features are used potentially for interpreting the weak molecular interactions. Ferrocenemethanol at a bulk concentration level of 0.5 mM adsorbs on the 3-mercaptopropanoic acid surface by hydrogen bonding, with the fractional surface coverage ranging from 0% to 14% of a monolayer in accordance with the surface coverage of 3-mercaptopropanoic acid which was explored by electrochemical quartz crystal microbalance. The intermolecular hydrogen bonding between ferrocenemethanol and the mercaptopropanoic acid was found to exhibit a Langmuir-type adsorption with the average value of K being 0.24 mM–1. The intermolecular hydrogen bonding between FcCH2OH and the artificial self-assembled monolayer (3-mercaptopropanoic acid) is evident, and the study on quantification of intermolecular hydrogen bonding with this artificial model is meaningful for understanding the molecular recognition events involved in the cell membrane.
研究领域:
电化学
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生物物理学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
CRYSTENGCOMM
·
2020
·Vol. 22
·期 34
·5628-5637
·
IF 3.117
Jinyue Yang,Na Wang,Beiqian Tian,Xiongtao Ji,Baohong Hou,Ting Wang,Xin Huang,Junquan Su,Zhanao Yang,Hongxun Hao
To investigate the effect of positional isomerism on cocrystallization and reveal the role of weak interactions in the formation of cocrystals, experimental characterization complemented with theoretical computations was thoroughly conducted by using p-hydroxybenzoic acid (PHBA), o-hydroxybenzoic acid (OHBA), m-hydroxybenzoic acid (MHBA) and malonic acid (MA) as model compounds. Selective formation of a new cocrystal was discovered between PHBA and MA molecules via several methods, while no cocrystal was formed between OHBA/MHBA and MA molecules. The PHBA–MA cocrystal was thoroughly characterized and analyzed through several solid characterization techniques such as powder X-ray diffraction (PXRD), differential scanning calorimetry (DSC) analysis, Fourier transform infrared (FTIR) spectroscopy, single crystal X-ray diffraction (SCXRD), etc. Taking advantage of a myriad of computational modelling techniques, the molecular recognition mechanism which drove the formation of cocrystals was comprehensively investigated and understood at a molecular level. The structural change accompanied by the cocrystal formation could be rationalized by FTIR spectroscopy and electron density variation analysis. Hirshfeld surface (HS) analysis indicated that O–H⋯O hydrogen bonds played a critical role in the formation of this cocrystal. Molecular electrostatic potential (MESP) surface analysis was employed to predict the interaction sites for cocrystallization, which were in good accordance with the experimental results. Moreover, possible reasons for the selective cocrystallization were analyzed and proposed.
研究领域:
化学
·
材料科学
·
晶体学
✓ 已验证
产品关联
DOI
高影响因子
Advanced Healthcare Materials
·
2024
·
IF 10
Meng-Meng Chen,Han-Wen Tian,Yuan-Qiu Cheng,Yu-Xin Yue,Yu-Chen Pan,Dong-Sheng Guo
Snakebite is a medical emergency that often leads to high mortality and morbidity in tropical and subtropical regions. The diversity in venom composition between various types of snakes necessitates the use of different specific antivenom treatments, which presents clinical and financial challenges in the management of snakebites. Therefore, developing a broad-spectrum antidote to neutralize complex and diverse snake venoms has assumed critical importance. Herein, a broad-spectrum antidote is developed through molecular recognition and coassembly to neutralize four kinds of snake venom toxins, those are three-finger toxin (3FTx), snake venom metalloproteinase (SVMP), phospholipase A 2 (PLA 2 ), and snake venom serine protease (SVSP), which have high abundance and toxicity. The coassembly of sulfonate-modified amphiphilic calixarene (SCA) and amphiphilic cyclodextrin (CD) is employed to neutralize the toxicity of 3FTx, 1,2-dodecanedithiol (DDT) and thioetheramide phosphorylcholine (TEAPC) are introduced through coassembly to neutralize toxicity of SVMP and PLA 2 , respectively, and the nafamostat (NT), which can be loaded through the host-guest complexation, is employed to neutralize toxicity of SVSP. The broad-spectrum antidote, NT@SCA/CD/DDT/TEAPC, effectively neutralizes key toxins from the most medically important cobra and vipers, significantly improving survival rates of poisoned mice. This study conceptually validated the feasibility of broad-spectrum detoxification and also offers a conveniently integrated supramolecular strategy.
研究领域:
医学
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生物化学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
WATER RESEARCH
·
2019
·
IF 7.913
Chao Shan,Bowen Yang,Bo Xin,Dandan Wang,Ping Huang,Zhicai Chen,Zhongbo Wei,Ming Hua,Weiming Zhang,Dionysios D. Dionysiou,Bingcai Pan
It has been long desired but challenging to forward the advanced treatment of wastewater from empirical trials towards scientific design due to the lack of molecular insight into the pollutants of concern. Herein, we first established a systematic methodology to identify the ligands of Ni(II)-complexes in an electroless nickel (EN) plating effluent. The presence of N-containing groups in the ligands of most Ni(II)-complexes was verified by time-aligned ICP-MS and ESI-HRMS, implying the suitability of autocatalytic ozonation for efficient decomplexation. Thereby, a combined process was proposed on the basis of ozonation to achieve over 83% decomplexation of Ni(II) (initially at 0.36 mg/L), followed by selective Ni(II) sequestration for resource recovery. Combinational LC-MS systems revealed the ozonation-induced fragmentation or elimination of most Ni(II)-complexes as well as the structural change of the residual complexed molecules. The released free Ni(II) was further sequestrated by a nanocomposite of hydrated Zr(IV) oxide confined in a polymeric cation exchanger (nHZO@PCE). The fixed-bed working capacity of nHZO@PCE (∼550 BV) for the ozonated EN plating effluent was over 18 times that of the cation exchanger host (∼30 BV) at the breakthrough point of 0.10 mg Ni/L. More attractively, five adsorption-regeneration cycles demonstrated the great potential of the hybrid adsorbent for sustainable utilization. This study is believed to shed new light on how to design rational processes for advanced treatment of real wastewater based on molecular identification.
研究领域:
环境科学
·
化学工程
✓ 已验证
DOI
PubMed
高影响因子
MRS Advances
·
2018
·Vol. 3
·期 28
·1583-1588
·
IF 0
Chen Jing,Yang Jingli,Gao Guorong,Fu Jun
Macroscopic assembling of responsive hydrogels has been used to construct soft actuators that transform their shape upon external stimuli. It remains a challenge to establish a robust assembling interface between gels. Here, we demonstrate a fabrication of bilayered hydrogel actuators assembled by host-guest recognition at the interface. The supramolecular recognition enabled efficient, rapid, and robust macroscopic assembling of hydrogels, which was utilized to create gel bilayers that were actuated upon unbalanced swelling/deswelling.
研究领域:
材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
Frontiers in Bioengineering and Biotechnology
·
2022
·
IF 6.064
Liu Xina,Zhu Meiqing,Xu Chenyang,Fan Fugang,Chen Panpan,Wang Yi,Li Dongyang
As an inorganic small molecule pollutant, the toxicity and potential carcinogenicity of hydrazine (N2H4) are of increasing concern. In this work, A water-soluble fluorescent probe (OCYB) based on the intramolecular charge transfer (ICT) mechanism for the detection of hydrazine was designed and synthesized. Taking the advantage of 4-bromobutyryl as the recognition group, the high selectivity of OCYB to N2H4 was confirmed by steady-state fluorescence spectroscopy. The limit of detection (LOD) was calculated to be 78 nM in the DMSO-HEPES (pH 7.4) system. The detection mechanism was verified by NMR, HRMS and density functional theory (DFT) calculations. In addition, OCYB exhibits strong anti-interference ability and an “Off-On” fluorescence enhancement effect. Importantly, OCYB can be used to effectively monitor the fluorescence distribution of N2H4 in environmental water samples and organisms.
研究领域:
分析化学
·
环境科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2018
·
IF 6.735
Shucheng Liu,Xuan Zhang,Yue Ma,Xue Bai,Xueping Chen,Jinxin Liu,Jianming Pan
By immobilization of nano-sized adsorbents into a water-rich matrix to fabricate a hybrid hydrogel has raised considerable attention in aqueous phase molecular recognition, owing to the combination of inherent advantages of easy operation, good mechanical performance and abundant recognizing sites by a light-triggered chemical polymerization strategy. The boronic acid and vinyl-functionalized multi-walled carbon nanotubes (VMWCNTs-COOH-BA) were introduced to hydrogel matrix, and then as-prepared hybrid hydrogels (MBA-H-5.0) were applied for selective adsorption and separation of luteolin (LTL). Surface hydrophilicity, mechanical performance and specific surface area of the hybrid hydrogels were enhanced by the addition of functional VMWCNTs-COOH-BA. In static adsorption, MBA-H-5.0 showed a large LTL loading amount (35.91 mg g −1 at 308 K), fast capture kinetics (3.0 h), remarkable selectivity, and excellent recyclability (8.24% loss in adsorption amount after seven adsorption-desorption cycles). The abundant boron-containing functional groups such as boronic acid played a vital role on adsorption, and the copolymerization of VMWCNTs-COOH-BA and hydrogel matrix prevented from the leakage of nanostructured adsorbents. This study sheds new light on the design of high performance hybrid hydrogel by embedding functional adsorbents for selective separation and enrichment of natural flavonoids.
研究领域:
材料科学
·
环境科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2025
·
IF 2.5
Jiaojiao Wang,Yanyan Gao,Yuexing Zhang,Dongmei Xu,Zongxin Li,Yifei Su,Fang Wang,Ning Zhang,Daopeng Zhang,Kang Wang,Yuting Chen
A versatile dihydroxylsalicylaldazine-containing fluorene (DHSF) compound was easily prepared through a one-step reaction. DHSF simultaneously exhibited solvent-regulating fluorescent OFF–ON behavior toward Al3+/Cr3+ and a dynamic OFF–ON–OFF fluorescent response toward Fe3+ in different aqueous DMF solutions with multiple absorption–ratiometric signals. These could be mainly attributed to the different binding modes between enol–keto isomers and Fe3+/Al3+/Cr3+, as proved by DFT calculation and IR spectroscopy results. Furthermore, DHSF displayed fluorescent OFF–ON–OFF and absorption–ratiometric dual responses to increasing ClO− amounts. These results, together with the fluorescent ON–OFF changes from the metal displacement of the DHSF–Al3+/Fe3+ system by Cu2+, show that monomolecular DHSF is a versatile chemosensor for sensitively detecting Al3+/Cr3+/Cu2+ and quantitatively analysing Fe3+/ClO− with practical application potential.
研究领域:
化学
·
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2016
·Vol. 408
·期 19
·5319-5328
·
IF 3.125
Ji Wenhua,Zhang Mingming,Gao Qianshan,Cui Li,Chen Lizong,Wang Xiao
Hydrophilic molecularly imprinted polymers (H-MIP) with molecular recognition ability for iridoid glycosides (IGs) have been obtained via bulk polymerization combined with hydrolysis of ester groups. H-MIP were characterized by Fourier transform infrared spectroscopy (FT-IR). The hydrophilcity was measured by the contact angle measurement and the water dispersion stability. The obtained H-MIP demonstrated high selectivity and specific binding ability to five IGs in aqueous media. The group extraction efficiency of molecular imprinted solid-phase extraction (MISPE) for five IGs was investigated, including loading sample, breakthrough volume, washing solvent, and elution solvent. Compared with non-imprinted solid-phase extraction (NISPE), the higher average recovery (95.5 %) of five IGs with lower relative standard deviations values (below 6.1 %) using MISPE combined with high-performance liquid chromatography (HPLC) were achieved at three spiked levels in three blank samples. Under the optimum MISPE conditions, the wide linear range with the correlation coefficient of R
2 ≥ 0.9950 for five IGs with low limits of detection (LOD) and quantification (LOQ) (0.01–0.08 and 0.03–0.27 μg mL −1 , respectively) were obtained. Chromatograms obtained using MISPE columns demonstrated that the matrix interference has been minimized and great interferences around IGs were also eliminated efficiently. These results indicated that the developed MISPE-HPLC method was selective, accurate, and applicable for the determination of IGs in water media.
Preparation of hydrophilic molecularly imprinted polymers via bulk polymerization combined with hydrolysis of ester groups
研究领域:
分析化学
·
药物分析
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）