Cell Reports Physical Science
·
2023
·
IF 8.9
Xueqi Wang,Kai Sun,Han Wang
Nucleic acid biomarkers play important roles in early diagnosis, routine management, and prognosis of diseases. However, current nucleic acid testing (NAT) approaches either require cumbersome instruments or lack sensitivity for short detection time, making point-of-care (POC) NAT challenging. Here, we report an ultrasensitive carbon fiber paper (CFP)-based electrochemical biosensor with one-step branched DNA (bDNA)-based molecular signal amplification system for rapid, sensitive, and low-cost replication-free NAT. For nucleic acid quantification, the proposed biosensor exhibits a wide linear range of 10 aM–1 μM and a low limit of detection (LOD) of 1.4 aM. For fast semi-quantitative detection, the biosensor can evaluate the concentration of nucleic acids within 10 min with a threshold as low as 1 fM. Furthermore, the biosensor demonstrates negligible batch-to-batch variation with a relative standard deviation (RSD) of 4.25% at the cost of less than $1/device. The present one-step bDNA-based electrochemical biosensor serves as a promising and universal method for rapid, sensitive, and portable NAT.
研究领域:
生物医学工程
·
分子诊断学
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
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IF 7.46
Jianhao Hu,Zhipeng Shen,Lei Tan,Jun Yuan,Ning Gan
In this work, we demonstrate a double stirring bars-based signal-amplified strategy for the simultaneous electrochemical detection of Vibrio parahaemolyticus (V.P) and Salmonella typhimurium (S.T). Methylene blue (MB) or ferrocene (Fc) labeled functionalized branched-chain DNA hybrid structure (FBCHS) was immobilized on magnetic microspheres and used as encoded signal tags. The encoded signal tags were hybridized with the aptamer-embedded tetrahedral DNA nanostructures (TDNs), which acted as capture probes, on one gold stirring bar (bar-A). The magnetic signal tags on bar-A could capture V.P and S.T, and their complex was later released into the solution. The composites were then digested by exonuclease I that was modified on another gold stirring bar (bar-B). The released free bacteria participated in the next cycle, from bar-A to bar-B, and generated more magnetic tags for signal amplification. After the reaction, the tags were magnetically enriched on a screen-printed carbon electrode (SPCE), generating two distinct voltametric peaks derived from MB and Fc. Their current intensities could reflect the concentration of V.P and S.T in the samples. Under optimal conditions, the double stirring bars-based signal amplification strategy was able to increase the detection sensitivity with a lower detection limit of 4 and 7 CFU/mL for V.P and S.T, respectively, within a detection range of 10–10 8 CFU/mL. Importantly, the exonuclease I on the stirring bar (bar-B) for signal amplification was able to be reused up to 50 times and avoided its loss if being directly added in solution, which produced cost savings in terms of used enzymes.
研究领域:
生物技术
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食品科学
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分子生物学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2023
·Vol. 24
·期 16
·12565
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IF 5.6
Zhikun Zhang,Chunyan Shang,Cuixia Hu,Yumin Liu,Jilong Han
A branched DNA-based electrochemical biosensor was designed to sensitively detect specific nucleic acids. On this platform, novel a branched DNA with three sticky ends could be used as a biosensor to sensitively and specifically detect nucleic acids. Meanwhile, we also employed branched DNA-modified AuNPs as a signal amplifier to further improve the sensitivity. Branched DNA sensors, target DNA, and DNA-modified AuNPs formed a sandwich structure to produce an electronic signal for target DNA detection. The reaction primarily involved DNA hybridization without bulky thermal cyclers and enzymes. We proved that the hybridization reaction easily occurred under different conditions, such as the NaCl concentration, reaction time, pH, and temperature, except for a pH lower than 4. The limit of detection could go as low as 0.09 pM (S/N = 3) with excellent specificity and selectivity. There was a correlation curve relationship between the peak current and the logarithm of the target DNA concentration (0.10 pM to 10 nM). The correlation coefficient reached 0.987. The electrochemical platform enables a branched DNA nanostructure to determine nucleic acids for disease diagnosis.Keywords:amplifier;branched DNA;diagnosis;detection;nucleic acids
研究领域:
生物技术
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纳米技术
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分析化学
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分子生物学
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DOI
PubMed
高影响因子
NANO
·
2020
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IF 1.566
Zhikun Zhang,Xiaojie Ye,Qingqing Liu,Cuixia Hu,Jimmy Yun,Runjing Liu,Yumin Liu
Nucleic acid detection is becoming increasingly important in the diagnostics of genetic diseases for biological analysis. We herein propose gold nanoparticles as probe for colorimetric detection of nucleic acids with branched DNA nanostructures, which enables a novel and simple colorimetric biosensor. In our system, the target DNA specifically triggered two short-chain ssDNA probes to generate branched DNA nanostructures (Y-shape DNA), which prevent AuNPs from aggregation in aqueous NaCl solution. On the contrary, when the target DNA did not exist, gold nanoparticles were unstable and aggregated easily because there is no anti-aggregation function from Y-shape DNA. Sensor response was found to be proportional to the target DNA concentration from 5 to 100 nM, with detection limits determined as 5 nM. The developed platform is for colorimetric nucleic acid detection without enzymes, label and modification holds great promise for practical applications.
研究领域:
生物技术
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纳米技术
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分子生物学
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分析化学
✓ 已验证
产品关联
DOI
高影响因子
International Journal of Electrochemical Science
·
2023
·
IF 1.5
Mingyi Ma,Li He,Xiaoxue Shi,Yanchao Wang,Hong Hai,Xiaoping Wei
This paper presents a novel strategy for ultrasensitive detection of human immunodeficiency virus (HIV) DNA by an electrochemical biosensor. By the shearing cycle of the target DNA (T-DNA), a large amount of complimentary sequence probes (CS probe DNA) are obtained. Then, the Zr 4+ functional probe DNA containing many branched DNAs is captured. Next, the initiator α-bromophenylacetic acid is connected to the branched chains of probe DNA by esterification reaction. Numerous electroactive monomers, namely, ferrocenylmethyl methacrylate (FMMA), are produced at the end of the branched chains by electrochemically mediated atomic transfer radical polymerization reaction. The signal of FMMA is greatly enhanced due to a large number of branched chain-loaded probe clusters containing numerous FMMA. T-DNA can be detected with a linear range of 0.1 fM–10 nM with a detection limit of 0.037 fM. The sensor is highly sensitive and selective. It has been applied to the detection of HIV DNA in human serum samples, and it has potential application prospects in the early clinical diagnosis of AIDS.
研究领域:
生物技术
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分析化学
·
病毒学
·
电化学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Daxiu Li,Feng Qiu,Ruo Yuan,Yun Xiang
Simultaneous detection and quantification of antimalarial drugs is important for evaluating the spread of their resistance. On the base of the target-induced assembly of branched DNA nanostructure signal amplification and different electrochemical coding tags at distinct potentials, an aptasensing method for multiplexed and highly sensitive detection of piperaquine (PQ) and mefloquine (MQ) antimalarial drugs is established. The target drugs associate with and switch the conformation of the aptamers to release free ssDNA trigger sequences, which initiate the self-assembly of many methylene blue (MB)- and ferrocene (Fc)-labeled auxiliary signal sequences to form branched nano-assemblies on electrode. The electrode-captured MB and Fc tags thus yield highly magnified current reponses at well-resolved potentials for simultaneously monitoring PQ and MQ with respective detection limits as low as 0.061 ng mL −1 (i.e., 0.114 nM) and 0.17 ng mL −1 (i.e., 0.449 nM). Moreover, the simultaneous detection of PQ and MQ in diluted human serum samples has also been verified by the developed sensor, revealing its potential for multiplexed monitoring of different drugs in connection with the corresponding aptamers for various application purposes.
研究领域:
分析化学
·
生物化学
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药学
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纳米技术
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）