FOOD AND BIOPRODUCTS PROCESSING
·
2024
·
IF 3.5
Lu Ren,Yang Hu,Yefei Wu,Mengqian Zhao,Qianqian Ma,Yulu Du,Jianwei Lu,Xuwei Long
Sophorose is a rare and valuable disaccharide with an unusual β -1,2 bond. Despite the relatively widespread interest in sophorose, the information regarding sophorose is limited because sophorose is not readily accessible. Thus, we used Candida bombicola ATCC 22214 for the efficient fermentative production of sophorose using glucose and soybean oil as substrates. In addition, an integrated separation process comprising precipitation, extraction, electrodialysis, and column chromatography was developed to purify the sophorose. Sophorose (approximately 35 g/L) was produced under optimized fermentation conditions in terms of the feeding strategy and enhanced mass-transfer efficiency. Thereafter, approximately 38% of sophorose (purity above 99%) was recovered from fermentation broth through the proposed process. This study provides an efficient approach for the large-scale preparation of sophorose, which is helpful for its practical application and in-depth research.
研究领域:
生物工程
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糖化学
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产品关联
DOI
高影响因子
JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
·
2022
·Vol. 331
·期 9
·3905-3913
·
IF 1.754
Guo Zong,Chu Taiwei
In this work, a separation and purification process of U(VI), Zr(IV) and I 2 from various fission products in nitric acid solution by 1-butyl-3-methylimidazolium bis(trifluoromethanesulfonyl)imide ([C 4 mim][NTf 2 ]) and dibutyl-3-(3-methylimidazolium)propylphosphoramide bis(trifluoromethanesulfonyl)imide-task specific ionic liquid (phosphoramide-TSIL) was investigated. The phosphoramide-TSIL was found to reduce ligand loss compared to dibutyl propylphosphoramidate by total organic carbon testing (TOC). The stripping of U(VI), Zr(IV) and I 2 could be achieved by guanidine carbonate solution, oxalic acid solution and sodium hydroxide solution, respectively. The phosphoramide-TSIL/[C 4 mim][NTf 2 ] showed good irradiation stability and recyclability. Phosphoramide-TSIL has the potential to be applied for separation and purification in the actual radioactive environment. Graphical abstract
研究领域:
核化学与放射化学
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环境科学
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产品关联
DOI
高影响因子
JOURNAL OF CHEMICAL RESEARCH
·
2023
·
IF 1.4
Ruilong Jia,Qizhuozhuo Hua,Xiaonan Ma,Guangfu Liao
Allyl alcohol and water can form the lowest boiling point azeotrope, but it is very hard to acquire high-purity allyl alcohol by normal distillation methods. Herein, a separation and purification protocol is developed for perfectly separating an azeotropic mixture of allyl alcohol and water using the salting-out method, in which three potassium phosphate salts, K5P3O10, K3PO4, and K4P2O7, are systematically investigated as salting-out agents, and finally a product consisting of > 99% allyl alcohol is obtained. A thermodynamic study demonstrates that this process involves endothermy and increment entropy. There is a good correlation between the solubility of allyl alcohol and the molar concentration of salt (mol per 1 kg water), and the solubility of the allyl alcohol in the organic phase or the water phase can be forecast using the mass percentage of salts. This work provides a new methodology for the efficient separation of an azeotropic mixture of allyl alcohol and water.
研究领域:
化学工程
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物理化学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2014
·
IF 3.259
Peng-Cheng Sun,Ying Liu,Yue-Tao Yi,Hong-Juan Li,Ping Fan,Chuan-Hai Xia
In the present study, a simple and efficient method for the preparative separation of 3-CQA from the extract of Helianthus tuberosus leaves with macroporous resins was studied. ADS-21 showed much higher adsorption capacity and better adsorption/desorption properties for 3-CQA among the tested resins. The adsorption of 3-CQA on ADS-21 resin at 25 °C was fitted best to the Langmuir isotherm model and pseudo-second-order kinetic model. Dynamic adsorption/desorption experiments were carried out in a glass column packed with ADS-21 to optimise the separation process of 3-CQA from H. tuberosus leaves extract. After one treatment with ADS-21, the content of 3-CQA in the product was increased 5.42-fold, from 12.0% to 65.2%, with a recovery yield of 89.4%. The results demonstrated that the method was suitable for large-scale separation and manufacture of 3-CQA from H. tuberosus leaves.
研究领域:
植物化学
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天然产物开发
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DOI
高影响因子
CHINESE JOURNAL OF CHEMICAL ENGINEERING
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2020
·
IF 2.627
Ling Meng,Xia Gui,Zhi Yun
Oridonin, one of the active ingredients in Rabdosia rubescens ( R. rubescens ), has been reported to induce cell apoptosis and cell cycle arrest in many cancers. Conventional extraction methods tend to result in unsatisfied enrichment and poor quality of oridonin present in a given biomass. This paper aims to evaluate the performance and separation characteristics of four different macroporous resins to arrive at the most suitable methodology for the isolation and purification of high-quality oridonin. Static absorption kinetics, thermodynamic and dynamic adsorption were evaluated. HP-20 was selected for further study due to its high adsorption capacity of 32 mg·g −1 and desorption ratio with 98.5%. The pseudo-second-order model was considered to be the most suitable for kinetic results, and Langmuir model was chosen to better describe the absorption thermodynamics. Under optimum conditions (flow rate of 4 ml·min −1 , bed depth with 6 cm and initial concentration of 2.15 mg·ml −1 ), the effective content of oridonin increased from 33.9% to 79.1% in the dry extract with a recovery of 81% and the purity of oridonin improved from 76% to 93%. The results confirm that HP-20 provides an efficient method to purify most oridonin from R. rubescens .
研究领域:
药用植物学
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药物化学
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生物工程
✓ 已验证
产品关联
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
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2017
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IF 3.359
Ying Zhao,Xinping Ouyang,Jing Chen,Lisha Zhao,Xueqing Qiu
The conversion of lignin into value-added aromatic monomers has attracted much attention as these monomers can be potentially used to prepare liquid fuel rather than heavy oil by further hydrodeoxygenation. A new process combining Sephadex and silica gel column chromatography was employed to obtain aromatic monomers with higher purity. It was found that pH and polarity of mobile phase were the most important factors affecting the separation effect for Sephadex G-10 and silica gel column chromatography, respectively. When the pH of mobile phase reached 10.5, the ionization of hydroxyl together with carboxyl groups resulted in electrostatic repulsion between the aromatic compounds and Sephadex G-10 gel, which counteracted the adsorption caused by hydrogen bond between aromatics and gel, causing the increase of the separation effect. Under the condition of pH above 10.5, Sephadex gel column chromatography gave 78.1% of purity of aromatic monomers (PAM) and 99.5% of recovery of aromatic monomers (RAM) due to the elimination of non-size exclusion effect. Silica gel column chromatography was further conducted to obtain 99.8% of PAM and 73.9% of RAM. The polar mobile phase was favorable for eluting aromatic monomers, while the non-polar mobile phase was beneficial to remove oligomers and increasing the PAM.
研究领域:
生物化学
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环境科学
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材料科学
✓ 已验证
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2023
·
IF 8.6
Xiaoyun Liu,Su Yan,Hua Zhou,Hao Wu,Shuya Wang,Xiaoyu Yong,Jun Zhou
Glabridin has beneficial antioxidant, anti-inflammatory, and tyrosinase inhibitory activities, with many applications in the pharmaceutical and cosmetics industries. After industrial extraction of glycyrrhizic acid, Glycyrrhiza glabra residue contains abundant glabridin. Glabridin was extracted from Glycyrrhiza glabra residue with a deep eutectic solvent (DES), followed by the recovery of the glabridin by organic solvent extraction, and then partial purification with hydrophobic macroporous resin. Ethyl acetate was the best extraction solvent, with an extraction yield of 91.63 %. Twelve macroporous resins were evaluated and LSA-21 yielded the best recovery of glabridin. Adsorption of glabridin by LSA-21 resin conformed to pseudo-second-order kinetic and Langmuir models. The thermodynamic parameters ΔH (enthalpy change) and ΔG (Gibbs free energy) were both negative, ΔS (entropy change) was positive. These results indicated that LSA-21 resin spontaneously and exothermically adsorbs glabridin to form a monolayer. The optimum conditions for separating and purifying glabridin by LSA-21 resin were 50 % ethanol aqueous solution(v/v) with a concentration of 56.90 µg/mL glabridin at a flow rate of 2 bed-volumes (BV)/h, and elution with 4 BV of ethanol at 2 BV/h, producing a glabridin yield of 97.80 %. The resin could be reused four times and the glabridin yield slightly decreased to 94 %. The overall recovery of glabridin from the DES Glycyrrhiza glabra extract solution after the ethyl acetate and LSA-21 steps was 89.61 %, with a purity of 17.38 %. This approach is an effective method for the recovery and purification of glabridin from a DES extract of Glycyrrhiza glabra residue, which has potential industrial application for producing glabridin and other natural plant products.
研究领域:
植物化学
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药学
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生物工程
✓ 已验证
DOI
高影响因子
JOURNAL OF POLYMER RESEARCH
·
2024
·Vol. 31
·期 9
·1-18
·
IF 2.6
Xu Wan,Zhang Huijuan,Huo Ting,Xiang Yongsheng,Ou Xiaojian,Sun Yuan,Sun Yuanjun,Chen Zhenbin
The excellent physical and chemical properties of palladium(Pd) have made its application and demand increase gradually. Therefore, it is important to develop a new material to efficiently separate and purify palladium from secondary sources to ensure its supply. In this work, a thermosensitive palladium smart imprinted polymer(Pd-T-SIP) was synthesized, which could achieve the efficient separation and purification of Pd. The microstructure and morphology of Pd-T-SIP were characterized. The experimental results showed that the Pd-T-SIP showed the maximum adsorption amount( Q ) of 0.1022 mmol/g for Pd(IV), the desorption rate( D ) was 83.09%, and it had good reusability. Theoretical studies showed that the adsorption process of Pd-T-SIP was suitable to be described by quasi-first-order model and Langmuir model, which indicated that the adsorption of Pd-T-SIP was monolayer adsorption. Finally, Pd-T-SIP was applied to the platinum group catalyst leach solution, and it could be found that its adsorption/desorption effect was more excellent, with its adsorption amount reaching 0.1558 mmol/L, desorption rate reaching 77.14%, and the palladium purity after one adsorption/desorption cycle increased from 13.75% to 30.62%, and the purity of platinum group metals increased from 40.29% to 73.56% after one adsorption/desorption cycle.
研究领域:
材料科学
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环境科学
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化学工程
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产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2018
·
IF 3.716
Guozhu Liu,Baolei Luan,Guiting Liang,Libo Xing,Liang Huang,Changyun Wang,Ying Xu
Capreomycin has good clinical utility to treat multidrug resistant tuberculosis, but it is only used as a second line drug due to its adverse reactions. Literature has demonstrated that the toxicity of capreomycin product is significantly influenced by the impurities in it. Unfortunately, so far, no one impurity in capreomycin has ever been isolated and definitely identified due to its extremely strong basic character and high polarity. An ion-pair method reported in literature can provide separation of capreomycin and its impurities, but it is hard to be used for the preparative purpose. In this study, this ion-pair method was further improved to detect more impurities in capreomycin sulfate substance. Besides the four main components (IA, IB, IIA and IIB), four impurities (impurity A–D) with their contents much higher than the identification threshold were observed. Furthermore, a two dimensional (2D) LC quadrupole-time of flight (Q-TOF) MS method was established to realize high resolution MS analysis of these impurities. For the purpose of preparative isolation, a hydrophilic interaction chromatography (HILIC) method was established. The four main components were well isolated, but unfortunately, the four impurities were co-eluted with each other or with IB by the HILIC method. Fortunately, the degradation experiments revealed that IA and IB could yield clean impurity A and B respectively in acidic medium, and can yield clean impurity D and C respectively in alkaline medium. Therefore, IA and IB were first isolated by the preparative HILIC method, then pure IA and IB underwent acid degradation and base degradation separately and followed by re-isolation by the HILIC method to obtain pure impurity A–D respectively. Based on Q-TOF MS and NMR analysis, the structures (including absolute configuration) of the four isolated impurities were definitely identified.
研究领域:
药物化学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
International Journal of Polymer Science
·
2020
·
IF 1.646
Xiaomin Zhang,Jie Sun
Chitosan sulfate was prepared and characterized as a new chromatography media for protein separation. The degree of sulfonation of chitosan could be well controlled and impacted under conditions in the synthesis process. The prepared chitosan sulfate shows improved binding capacity with proteins. Sulfonated chitosan shows improved ion-exchange adsorption properties with proteins, which could have good potential in protein purification.
研究领域:
生物化学
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材料科学
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蛋白质工程
✓ 已验证
DOI
高影响因子
ACS Applied Polymer Materials
·
2024
·
IF 4.4
Wan Xu,Bingqian Wang,Yuan Sun,Xiaojian Ou,Yuanjun Sun,Peng Zhang,Zhenbin Chen
Ruthenium, one of the platinum group elements, plays a crucial role in industrial development, and it is important to develop a material to separate and purify ruthenium with high efficiency to ensure its supply. Herein, a thermoresponsive Ru(III)-imprinted polymer (Ru(III)-TIIP) was prepared by combining thermoresponsive technology, Pickering emulsion technology, and imprinted technology. The Ru(III)-TIIP could regulate its structure by controlling the external temperature and maintained good adsorption and separation performance. The structure and performances of Ru(III)-TIIP were tested and evaluated by Fourier transform infrared, scanning electron microscopy, energy-dispersive spectrometry, transmission electron microscopy, specific surface area, porosity analysis, zeta potential, X-ray photoelectron spectroscopy, thermogravimetric analysis, and inductively coupled plasma-atomic emission spectrometry. The results showed that the maximum adsorption capacity (Q) of Ru(III)-TIIP was 0.2203 mmol/g under 33 °C. The maximum desorption rate (D %) was 78.1%, which was reached at 25 °C. When Fe(III) was used as the interfering ion, the separation degree (R) of Ru(III)-TIIP was 3.2. Meanwhile, the study of the adsorption process of Ru(III)-TIIP to Ru(III) was more suitable to be expressed by pseudo-first-order kinetics and the Langmuir model. When Ru(III)-TIIP was applied to the platinum group catalyst leach solution, the content of platinum group elements ranged from 53.72 to 82.09% after one adsorption/desorption cycle. In addition, Ru(III)-TIIP had maintained 66.14 and 81.25% of the original capacity for Q and R after 11 adsorption/desorption cycles.
研究领域:
材料科学
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环境科学
✓ 已验证
产品关联
DOI
高影响因子
Chemical Engineering and Processing-Process Intensification
·
2022
·
IF 4.264
Mengfei Tian,Xianming Meng,Naveed Ahmad,Chunying Li,Zhanyu Yuan,Zidan Luo,Yu Zhang,Chunyu Liang,Chunjian Zhao
Juglone was first preliminary purified by back extraction (BE) which also completed the sustainability of microemulsion as a solvent for extraction of juglone in J. mandshurica Maxim. branches. Then, magnetic solid phase extraction (MSPE) based on bubble column (BC) was proposed to further purify the juglone, GO/ND/Fe 3 O 4 as adsorbent, which broke through the limitation that MSPE is only suitable for trace enrichment. Thus, a BE combined with MSPE based on BC (BE-MSPE/BC) technology to separate and purify juglone from J. mandshurica Maxim. was developed. Under the optimal conditions (optimal adsorption conditions: 0.5 h of adsorption time, 30 mg/mL of solid-liquid ratio, 5.5 of pH, 60 L/min of air flow; optimal desorption conditions: 0.4 h of desorption time, methanol of desorption agent, 100% of desorbent volume fraction, 8 of pH, 40 mg/mL of solid-liquid ratio, 80 L/min of air flow rate), the recovery yield and purity of juglone obtained by BE-MSPE/BC were 80.57% and 72.38%, respectively. BE-MSPE/BC, as a green separation and purification technology that can easily achieve large-scale and sustainable production, is expected to be an alternative method to separate and purify active components in biomass materials and products.
研究领域:
植物化学
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分析化学
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环境科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）