Journal of Oceanology and Limnology
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2023
·Vol. 41
·期 5
·1965-1977
·
IF 1.6
Liu Yan,Kociolek John Patrick,Kulikovskiy Maxim,Glushchenko Anton,Yu Pan,Wang Quanxi,Lu Xinxin,Fan Yawen
An interesting group of cymbelloid diatoms was collected from lakes in Yunnan Province, China, and observations on their valve morphology made using light and scanning electron microscopy. These cymbelloid species all have apical pore fields, slit-like areolae, and areolar occlusions, and they all lack of stigmata. In this paper we propose the new genus Qinia gen. nov. for these taxa and describe them as new: Q. lashii sp. nov., Q. aequalis sp. nov., and Q. daliensis sp. nov. The genus Qinia gen. nov. has lanceolate valves asymmetrical about their apical axes, raphe located in the middle of valve, distal raphe ends bent to the dorsal margin that bisects the apical pore fields (APFs), no stigmata, areolae with slit-like external openings and projections occluding the areolar openings internally. This genus resembles the genus Cymbella in the presence of APFs, but differs from Cymbella by a lack of stigmata, and by having external distal raphe ends that bisect the apical pore fields. Like Reimeria , the external distal raphe ends bisect the lobes of the APF, but Qinia species differ from Reimeria in that they have no stigmata and the distal raphe ends are deflected dorsally, not ventrally. Comparisons were made between Qinia and other cymbelloid genera whose members possess apical pore fields. Features that distinguish each species of the new genus are also discussed.
研究领域:
植物分类学
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淡水生态学
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藻类生物学
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形态学
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系统发育研究
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BIOCHEMICAL SYSTEMATICS AND ECOLOGY
·
2025
·
IF 2
Li-Juan Hu,Xian-Sheng Ye,Kuan Lin,Jie Gao,De-Sen Yang,Guo-Ping Gan,Xi-Ji Shu,Wei Liu,You-Hua Yang
One previously undescribed 2-arylbenzofuran derivative ( 1 ), three stilbenes ( 2 - 4 ), three acetophenone glycosides ( 5 – 7 ), three phenylpropanoids ( 8 – 10 ), and eight flavonoids ( 11 – 18 ) were isolated from the rhizome of Smilax china L. in this study. NMR spectra elucidated their structures and were compared with previously reported data. Compounds 1 , 5 – 7 , 10 , and 18 were identified for the first time in Smilax china L. The cytotoxicity of the isolated compounds was detected on HeLa cells by the CCK-8 method, and compound 4 was found to possess the highest cytotoxic activity with an IC 50 value of 34.81 ± 1.50 μg/mL. Further studies revealed that the cytotoxic effect of compound 4 on HeLa cells was related to apoptosis and the cell cycle. In addition, the chemotaxonomic significance of the isolated compounds was discussed.
研究领域:
植物化学
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药理学
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细胞生物学
·
化学分类学
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BIOCHEMICAL SYSTEMATICS AND ECOLOGY
·
2018
·
IF 0.847
Latif Ahmad,Yi He,Andrew J. Semotiuk,Quan-Ru Liu,Jia-Chen Hao
Family Boraginaceae has drawn the attention of many botanists for many years because of its systematic position and the presence of toxic pyrrolizidine alkaloids (PAs). Some researcher used these alkaloids as chemotaxonomic markers at the family and subfamily level in several botanical families. Therefore, this explorative study carried out with an aim to determine the distribution of selected PAs and their chemotaxonomic significance in the tribe Lithospermeae. For this, Lithospermeae species along with other Boraginaceae members from different parts of Pan-Himalayan regions in Pakistan and China were brought to Beijing Normal University in Beijing, China. For HPLC fingerprint analysis, dried silica gel aerial parts (500 mg) of twenty-four mature flowering species of Lithospermeae and the available PAs: supinine, heliotrine, lycopsamine, europine, and echimidine were used as chemotaxonomic markers. In the laboratory, the plants of Lithospermeae were investigated for pyrrolizidine alkaloids by using high performance liquid chromatography (HPLC). Five pyrrolizidine alkaloids were identified qualitatively in 24 species of tribe Lithospermeae from Pan-Himalaya. The results of the study support the previous research that PAs can be used as a taxonomic marker. The PAs lycopsamine and echimidine were common in all Onosma species except O. sinicum Diels, O. waddellii Duthie, and O. griffithii Vatke. So we proposed that lycopsamine and echimidine can be used as taxonomic markers for Onosma species from Pan-Himalayan regions. In the case of the genus Maharanga , M. emodi (Wall.) A. DC. and M. bicolor (Wall. ex G. Don) A. DC. show the same composition of PAs lycopsamine and echimidine. But these two species could be easily distinguished from each other; heliotrine is present in M. emodi but absent in M. bicolor . Arnebia guttata Bunge was the only species in the genus Arnebia which was detected for only europine. A. benthamii (Wall. ex) G. Don. I.M. Johnst. and A. euchroma (Royle) I.M. Johnst. were similar in PA composition for heliotrine, lycopsamine, and echimidine. These two species can easily be distinguished from each other: europine is present in the former genus but absent in the latter one. Moreover, the result of HPLC fingerprint analysis shows that supinine is distributed throughout all investigated species from the Pan-Himalayan regions except Lithospermum arvense L. So we proposed that supinine could serve as a chemotaxonomic marker in the tribe Lithospermeae.
研究领域:
植物学
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药理学
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International Journal of Electrochemical Science
·
2023
·Vol. 17
·期 10
·221060
·
IF 1.5
Zhiguo Lu,Yuhong Zheng,Yin Shen,Li Fu,Lichuan Zhan,Wenbin Zhang
Michelia belongs to Magnoliaceae and has important timber, medicinal, oil and ornamental values. The wide morphological variation of this genus and the hybridization among some species make it difficult to classify. There are controversies about the systematic relationships and status of species within the genera. In this communication, we have made the first taxonomic survey of species of the genus Michelia using electrochemical fingerprinting. The molecules corresponding to the oxidation peaks in the electrochemical fingerprints were explored. The electrochemical fingerprints collected under different conditions were used to construct density pattern. Texture features and local binary pattern algorithms are used to the identification of different species. The results of the phylogenetic tree generated based on electrochemical fingerprinting differed significantly from those of traditional morphological taxonomy. However, these results are in good agreement with the phylogenetic trees generated by chloroplast sequencing.
研究领域:
植物系统学
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分析化学
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分子系统学
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化学生物学
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植物分类学
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BIOCHEMICAL SYSTEMATICS AND ECOLOGY
·
2025
·
IF 2
Xuelin Chen,Hanlei Wang,Kun Zhang,Jing Lu,Yumei Zhang
For the phytochemical studies of the fruits of Kochia scoparia (L.) Schrad. (Kochiae Fructus, KF), we isolated chemical components from KF. Twenty compounds, including six alkaloids ( 1 – 6 ), nine polyphenols ( 7 – 15 ), three flavonoids ( 16 – 18 ), and two sterols ( 19 – 20 ) were isolated and identified from the ethanol extract of KF by chromatographic and spectroscopic techniques. Compounds 2 , 3 , and 5 – 16 were isolated from KF and Kochia genus for the first time. Furthermore, by discussing the taxonomic significance of these secondary metabolites, we found that Araliaceae, Compositae, Ranunculaceae, and Leguminosae might have a close evolutionary relationship. In conclusion, these results provide new insights into the chemical diversity and chemotaxonomic significance of K. scoparia in the Kochia genus and the Chenopodiaceae family.
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Marine Drugs
·
2021
·Vol. 19
·期 4
·192
·
IF 5.118
Pengrui Wang,Jiapeng Chen,Lujing Chen,Li Shi,Hongbing Liu
Plant volatile organic compounds (VOCs) represent a relatively wide class of secondary metabolites. The VOC profiles of seven seaweeds (Grateloupia filicina,Polysiphonia senticulosa,Callithamnion corymbosum,Sargassum thunbergii,Dictyota dichotoma,Enteromorpha proliferaandUlva lactuca) from the Yellow Sea of China were investigated using multifiber headspace solid phase microextraction coupled with gas chromatography–mass spectrometry (HS-SPME/GC–MS), among them, the VOCs of three red algae Grateloupia filicina, Polysiphonia senticulosa, and Callithamnion corymbosum were first reported. Principal component analysis (PCA) was used to disclose characteristic categories and molecules of VOCs and network pharmacology was performed to predict potential biomedical utilization of candidate seaweeds. Aldehyde was found to be the most abundant VOC category in the present study and (E)-β-ionone was the only compound found to exist in all seven seaweeds. The chemical diversity of aldehydes inE. proliferasuggest its potential application in chemotaxonomy and hinted that divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS) fiber is more suitable for aldehyde extraction. VOCs inD. dichotomawere characterized as sesquiterpenes and diterpenes and the most relevant pharmacological pathway was the neuroactive ligand–receptor interaction pathway, which suggests thatD. dichotomamay have certain preventive and therapeutic values in cancer, especially in lung cancer, in addition to neuropsychiatric diseases.Keywords:volatile organic compounds;seaweeds;characteristic VOCs;chemotaxonomy;biomedical utilization;headspace solid phase microextraction
研究领域:
植物化学
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药理学
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海洋生物学
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PubMed
高影响因子
Plant Methods
·
2017
·Vol. 13
·期 1
·1-11
·
IF 3.51
Zhang Heng,Chen Zhenyi,Li Taihao,Chen Na,Xu Wenjie,Liu Shupeng
Background
Flos Chrysanthemi , as a part of Chinese culture for a long history, is valuable for not only environmental decoration but also the medicine and food additive. Due to their voluminously various breeds and extensive distributions worldwide, it is burdensome to make recognition and classification among numerous cultivars with conventional methods which still rest on the level of morphologic observation and description. As a fingerprint spectrum for parsing molecular information, surface-enhanced Raman scattering (SERS) could be a suitable candidate technique to characterize and distinguish the inter-cultivar differences at molecular level. Results SERS spectra were used to analyze the inter-cultivar differences among 26 cultivars of Chinese ornamental Flos Chrysanthemum . The characteristic peaks distribution patterns were abstracted from SERS spectra and varied from cultivars to cultivars. For the bands distributed in the pattern map, the similarities in general showed their commonality while in the finer scales, the deviations and especially the particular bands owned by few cultivars revealed their individualities. Since the Raman peaks could characterize specific chemical components, those diversity of patterns could indicate the inter-cultivar differences at the chemical level in fact. Conclusion In this paper, SERS technique is feasible for distinguishing the inter-cultivar differences among Flos Chrysanthemum . The Raman spectral library was built with SERS characteristic peak distribution patterns. A new method was proposed for Flos Chrysanthemum recognition and taxonomy.
研究领域:
植物学
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分析化学
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光谱学
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分子系统学
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中药鉴定
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CURRENT MICROBIOLOGY
·
2025
·Vol. 83
·期 2
·112
·
IF 2.6
Gao Peng,Lu Xie-Feng,Yao Ling,Xu Yuan-Jia,Zhang Shu-Yi,Liu Guo-Hong,Zhou Shun-Gui
Two novel aerobic, rod-shaped, motile bacterial strains, designated as strains sgz302251 T and sgz5001026 T , were both isolated from paddy soils. 16S rRNA gene sequences analysis revealed that strain sgz302251 T exhibited 98.3% similarity with its closest reference strain Paenibacillus vietnamensis N4 T , while strain sgz5001026 T had the highest similarity of 97.5% to Ectobacillus polymachus KACC 18242 T . The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strains sgz302251 T , sgz5001026 T and their closest reference strains were 86.0% and 21.2%, 84.9% and 22.9%, respectively. The genomic DNA G + C contents of sgz302251 T and sgz5001026 T were 63.4% and 68.8%, respectively. Strain sgz302251 T grew at 20–37 °C (optimum 30 °C), pH 5.0–9.0 (optimum 6.5–7.5) and tolerated 0–1.0% NaCl (optimum 0.5%, w/v), while strain sgz5001026 T grew at 4–45 °C (optimum 37 °C), pH 5.0–9.0 (optimum 6.0) and 0–0.5% NaCl (optimum 0%). The quinone in both strains was MK-7 and major polar lipids were diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE) and phosphatidylglycerol (PG). The main fatty acids (> 5%) of strain sgz302251 T were anteiso-C 15:0 , anteiso-C 17:0 , and C 16:0 , whereas strain sgz5001026 T included anteiso-C 15:0 , iso-C 15:0 , iso-C 16:0 and iso-C 14:0 . Based on the above evidence, the proposed names of the two novel species are Paenibacillus praedii sp. nov. (type strain sgz302251 T = MCCC 1K09633 T = KCTC 43808 T ), Ectobacillus soli sp. nov. (type strain sgz5001026 T = MCCC 1K09373 T = KCTC 25903 T ), respectively.
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Bio-protocol
·
2024
·Vol. 14
·期 21
·e5104
·
IF 1
Peng-Wu Yin,Xian-Guo Guo,Wen-Yu Song,Wen-Ge Dong,Yan Lv,Dao-Chao Jin
The ectoparasites of rodents and other small mammals usually involve five categories of arthropods—fleas, sucking lice, gamasid mites, chigger mites, and occasionally, ticks. These ectoparasites are medically important, serving as vectors for diseases such as plague, murine typhus, scrub typhus, forest encephalitis, Lyme disease, and other zoonoses. Field surveys, collection, and specimen preparation of ectoparasites are crucial for studying taxonomy, faunistics, ecology, and epidemiology. They are also essential for vector surveillance. The present protocol summarizes the on-site monitoring and specimen-making of ectoparasites of rodents and other sympatric small mammals. Besides the collection and specimen preparation of small mammal hosts, the protocol describes in detail the collection, fixation, specimen-making, and taxonomic identification of ectoparasites and provides some monitoring indices. The on-site monitoring indices include the host density index and the infestation indices of ectoparasites (prevalence, mean abundance, mean intensity). The methodologies outlined in this protocol provide technical guidance and references for vector monitoring (surveillance) and control. Key features • Collection and specimen preparation of small mammal hosts, including rodents (rats, mice, and voles) and other sympatric small mammals (shrews, tree shrews, and pikas). • Collection, fixation, specimen-making, and taxonomic identification of ectoparasites, including fleas, sucking lice, gamasid mites, chigger mites, and ticks. • On-site monitoring indices—host density index and infestation indices of ectoparasites: prevalence, mean abundance, and mean intensity. Graphical overview Flowchart for collection and specimen-making of ectoparasites and their hosts. A. Process for the collection and specimen preparation of small mammal hosts. B. Collection and fixation of ectoparasites. C. Process for specimen-making for different categories of ectoparasites.
研究领域:
寄生虫学
·
动物学
·
流行病学
·
公共卫生
·
医学昆虫学
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Insects
·
2025
·Vol. 16
·期 11
·1147
·
IF 2.9
Feiyang Liang,Wei Zeng,Sheng Li,Xingyue Liu
Bark lice are common in terrestrial ecosystems, yet the poor taxonomic study of them renders species identification difficult. In this study, we investigated the species identification of the bark louse genus Neostenopsocus using DNA barcodes (675 bp fragment of the mitochondrial COI gene). We examined newly collected specimens and the type materials of some valid species of Neostenopsocus , redescribing 13 valid species and proposing 39 new synonyms. A total of 110 sequences of Neostenopsocus species were obtained from newly collected specimens and public data. The results show that the COI barcode is sufficient to distinguish most Neostenopsocus species, whereas more molecular marks and distribution data are necessary to identify the closely similar species. The family Stenopsocidae exhibits a rich biodiversity in China, yet species identification based on morphological characters remains problematic due to insufficient study and absent molecular data. DNA barcoding has been widely employed for rapid species identification in insects, particularly through standardized COI gene sequencing. In this study, we conducted species delimitation for Neostenopsocus in China based on the morphological characters and DNA barcodes, comprising 20 morphological species with 110 barcodes. We redescribed 13 species from China and proposed 39 new synonyms. This study supports the applicability of DNA barcoding for species identification in Neostenopsocus . The K2P distance between the morphological species of Neostenopsocus ranges from 0.0051 to 0.2040, with most exceeding 0.10. The maximum intraspecific divergence reaches 0.0778 in N. nepalensis . The external morphology of Neostenopsocus shows considerable structural uniformity, and the genitalia provide limited diagnostic value for species identification. Instead, the marking patterns of the head and forewing are considered to be useful for species identification. Therefore, delimiting closely related species demands an integrated taxonomy approach that combines extensive geographical sampling, detailed morphological analysis, and nuclear DNA data.
研究领域:
昆虫学
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分子系统学
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分类学
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生物多样性
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DNA条形码
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COLEOPTERISTS BULLETIN
·
2017
·Vol. 71
·期 2
·211-258
·
IF 0.496
Stewart B. Peck,Alfred F. Newton
Presented is an annotated catalog of the 38 genera and 382 species of Leiodidae known to occur in the Nearctic Region of North America north of Mexico (the continental USA and Canada). Information on types, distributions, and biologies is included. Twelve of the species also or probably occur in Mexico, and 16 of the species also occur in Europe and/or Asia (i.e., are Holarctic in distribution). Ptomaphagus (Appadelopsis) neojonesi Peck and Newton is proposed as a nomen novum (replacement name) for Ptomaphagus (Adelops) jonesiPeck, 1978, preoccupied by Ptomaphagus valentinei jonesiJeannel, 1949. Stereus arenariusPeck and Cook, 2009 is transferred to Pseudotriarthron as P. arenarium (Peck and Cook, 2009), new combination.
研究领域:
昆虫学
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分类学
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生物地理学
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系统学
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MOLECULAR PHYLOGENETICS AND EVOLUTION
·
2004
·
IF 2.826
Joseph Hughes,Alfried P Vogler
We considered the contribution of two mitochondrial and two nuclear data sets for the phylogenetic reconstruction of 22 species of seed beetles in the genus Curculio (Coleoptera: Cuculionidae). A phylogenetic tree from representatives found on various hosts was inferred from a combined data set of mitochondrial DNA cytochrome oxidase subunit I, mitochondrial cytochrome b , nuclear elongation factor 1α, and nuclear phosphoglycerate mutase , used for the first time as a molecular marker . Separate parsimony analyses of each data set showed that individual gene trees were mainly congruent and often complementary in the support of clades but the analysis was complicated by failure of PCR amplification of nuclear genes for many taxa and hence missing data entries. When the four gene partitions were combined in a simultaneous analysis despite the missing data, this increased the resolution and taxonomic coverage compared to the individual source trees. Alternative approaches of combining the information via supertree methodology produced a comparatively less resolved tree, and hence seem inferior to combining data matrices even in cases where numerous taxa are missing. The molecular data suggest a classification of the European species into two species groups that are in accordance with morphological characteristics but the data do no support any of the previously recognised American species groups.
研究领域:
分子系统发育
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昆虫学
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进化生物学
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遗传学
·
分类学
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高影响因子
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