Royal Society Open Science
·
2018
·Vol. 5
·期 8
·
IF 2.504
Ning Chen,Weixia Li,Shuchao Wu,Yan Zhu
A novel method combining high-performance liquid chromatography with online post-column electrochemical derivatization and fluorescence detection was established for the detection of reserpine in mouse serum. Reserpine separation was conducted using a C18 column with 5 mM H3PO4 and acetonitrile (55/45, v/v) as eluent. Reserpine was then electro-oxidized into a strongly fluorescent compound using an electrolytic cell device. Detection parameters, such as potential and fluorescence wavelength, were optimized. The linearity of the proposed method ranged from 0.01 to 5.0 mg l−1 with a correlation coefficient of 0.9997. The limit of qualification (S/N = 10) and limit of detection (S/N = 3) were 9.7 and 2.9 µg l−1, respectively. Resperine recoveries from spiked blank and drug-treated mouse serum samples ranged from 92.0 to 115%.
研究领域:
分析化学
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药理学
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产品关联
DOI
PubMed
高影响因子
PHARMACEUTICAL BIOLOGY
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2020
·
IF 2.971
Lan Zhang,Yue Yang,Lei Di,Jun-long Li,Ning Li
Context In folk medicine, erxian decoction (EXD) is used to treat perimenopausal syndrome in women. It is also used clinically to treat depression, but the mechanism remains unknown.Objectives To investigate the neuroprotective effect of EXD, and its antidepressant potential.Materials and methods ICR mice were treated with EXD (0.5, 1.5 and 4.5 g/kg i.g.) and fluoxetine (6.0 mg/kg i.g.) for 10 days. On day 10 of the treatment, depression-like behaviour was induced by reserpine (2.5 mg/kg injected i.p.), and after 24 h of reserpine administration, it was assessed using the tail suspension and forced swimming tests. MTT assay, lactate dehydrogenase test, flow cytometry analysis, Hoechst staining and western blotting were used to assess the apoptosis of PC12 cells. Apoptosis proteins and neurotransmitter were tested in vitro and in vivo, respectively.Results MTT assay results showed corticosterone prevented cell growth, but EXD at concentrations of 100, 200 and 400 μg/mL restored cell viability (EC50: 204.016 μg/mL). EXD decreased lactate dehydrogenase leakage from 63.48 to 43.60 U/L, and upregulated expression of Bcl-2 while the expression of Bax, caspase-3 and caspase-8 were decreased in vivo and in vitro. Moreover, EXD improved depression-like behaviour in mice, and 4.5 g/kg EXD treatment increased the secretion of serotonin, dopamine and norepinephrine by 67.44, 28.12 and 42.12 pg/mg, respectively, in hypothalamus compared to that of reserpine group.Discussion and conclusions EXD demonstrated neuroprotective effects and improved depression-like behaviour in mice. Further research should be focussed on the mechanism of the active components in EXD.
研究领域:
药理学
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神经科学
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中医
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DOI
PubMed
高影响因子
Biomed Research International
·
2019
·
IF 2.197
Yan Chen,Jiongshan Zhang,Mengting Liu,Zengcheng Zou,Fenglin Wang,Hao Hu,Baoguo Sun,Shijun Zhang
Background. Accumulating evidence from prospective epidemiological studies has showed that depression disorder (DD) is a risk factor for cancer. The aim of this study is to explore the association of DD and the overall occurrence risk of hepatocellular carcinoma (HCC) and the mechanism. Methods. In this study, 60 mice were randomly divided into four groups: Control group, DD group, HCC group, HCC-DD group. Mice received a chronic dose of reserpine to establish depression model, followed by Diethylnitrosamine and Carbon tetrachloride administration to establish HCC models. Behavioral depression was assessed by sucrose preference test (SPT) and the expression of Serotonin 1A (5-HT1A) receptor in the hippocampal. The expression of Oatp2a1 and Oatp2b1 in the digestive system tissues was detected by PCR and western blotting. Results. Reserpine-administrated mice had a reducing sucrose preference at Day 14 compared with blank mice (P<0.05). The expression of 5-HT1A receptor in the hippocampal was decreased in DD mice compared with blank mice. The survival analysis indicated that the HCC mice with DD have poorer survival rate compared with the HCC mice. Compared with HCC mice, the expression of Oatp2a1 and Oatp2b1 was lower in liver and stomach tissue and higher in hepatic carcinoma and colon tissue of HCC-DD mice (P<0.05), and the expression of Oatp2a1 was higher in the spleen tissue of HCC-DD mice while the expression of Oatp2b1 was lower (P<0.05). However, no difference was found in the expression of Oatp2a1 and Oatp2b1 in the small intestine tissue between HCC group and HCC-DD group. Conclusions. DD was the adverse factors for the overall occurrence risk of HCC. Mechanistically, be the downregulation of Oatp2a1 and Oatp2b1 in liver tissue induced by DD might be involved.
研究领域:
癌症研究
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分子生物学
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药理学
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神经科学
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产品关联
DOI
PubMed
高影响因子
Natural Product Communications
·
2022
·
IF 1.496
Minghui Tang,Yong Ai,Siyang Zhu,Ni Song,Xian Xu,Leyi Liang,Baoshan Rong,Xiaomin Zheng,Lanyue Zhang,Tinggang He
Citrus reticulata Blanco has been widely used to cure some diseases such as cold, cough and indigestion. This study is aimed at determining the antidepressant-like effect of C. reticulata essential oils (CREOs) in reserpine-induced depression mice, as well as its possible mechanisms. The compositions of CREOs are firstly analyzed by gas chromatography-mass spectrometry (GC-MS), in which d-limonene is the main component. Moreover, the results from the forced swimming and tail suspension tests show that the inhalation of CREOs can significantly improve the depressive behavior of reserpine-induced depressed mice by reducing the weight of the mice and shortening the immobile time. After sniffing CREOs, the number of normal neurons in the hippocampus of reserpine-induced depressed mice is greatly increased. In addition, CREOs significantly increase the expression level of 5-hydroxytryptamine-1A receptors (5HT-1A), glucocorticoid receptors (GR) and brain-derived neurotrophic factor (BDNF) in the reserpine-treated mice brain tissue. Thus, these results have indicated that CREOs can be potential materials for drug and food development against depression.
研究领域:
药理学
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神经科学
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传统医学
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DOI
高影响因子
CELL RESEARCH
·
2024
·Vol. 34
·期 1
·47-57
·
IF 28.1
Wang Yuwei,Zhang Pei,Chao Yulin,Zhu Zhini,Yang Chuanhui,Zhou Zixuan,Li Yaohui,Long Yonghui,Liu Yuehua,Li Dianfan,Wang Sheng,Qu Qianhui
Monoamine neurotransmitters such as serotonin and dopamine are loaded by vesicular monoamine transporter 2 (VMAT2) into synaptic vesicles for storage and subsequent release in neurons. Impaired VMAT2 function underlies various neuropsychiatric diseases. VMAT2 inhibitors reserpine and tetrabenazine are used to treat hypertension, movement disorders associated with Huntington’s Disease and Tardive Dyskinesia. Despite its physiological and pharmacological significance, the structural basis underlying VMAT2 substrate recognition and its inhibition by various inhibitors remains unknown. Here we present cryo-EM structures of human apo VMAT2 in addition to states bound to serotonin, tetrabenazine, and reserpine. These structures collectively capture three states, namely the lumen-facing, occluded, and cytosol-facing conformations. Notably, tetrabenazine induces a substantial rearrangement of TM2 and TM7, extending beyond the typical rocker-switch movement. These functionally dynamic snapshots, complemented by biochemical analysis, unveil the essential components responsible for ligand recognition, elucidate the proton-driven exchange cycle, and provide a framework to design improved pharmaceutics targeting VMAT2.
研究领域:
神经科学
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药理学
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结构生物学
✓ 已验证
DOI
PubMed
高影响因子
CHEMISTRY & BIODIVERSITY
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2025
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IF 2.5
Yanqing Ma,Shuanghe Wang,Shanshan Luo,Yixi Zeng,Guofeng Shi,Lanyue zhang,Hui Li
Depression, a prevalent and debilitating mood disorder, remains a significant challenge in clinical treatment. Although pharmacological approaches targeting monoamine dysfunction have demonstrated efficacy, they are often accompanied by limitations and side effects. In this study, reserpine-induced depressed mouse models were used to investigate the antidepressant potential of five essential oils (EOs) derived from Jasminum sambac (JSEOs), Rosa rugosa , Magnolia denudata , Aloysia citriodora (ACEOs), and Abies balsamea . The chemical compositions of the five EOs were analyzed using gas chromatography-mass spectrometry (GC-MS). Subsequently, experimental mice inhaled the EOs for 1 h per day, followed by behavioral assessments using the forced swim test (FST) and tail suspension test (TST). Nissl staining was employed to assess the abundance of normal neurons in the mouse brain. At the same time, immunohistochemistry (IHC), Western blot, and reverse transcription-quantitative polymerase chain reaction techniques were utilized to analyze the expression of antidepressant-related genes. GC-MS results revealed that the primary components of the five EOs were oxygenated monoterpenes, sesquiterpene hydrocarbons, and oxygenated sesquiterpenoids. All five EOs exhibited antidepressant-like effects, with JSEOs and ACEOs demonstrating the greatest antidepressant efficacy. Behavioral tests showed a reduction in immobility durations in both FST and TST. Nissl staining results indicated an increase in the number of intact neurons in the hippocampus and hypothalamus of mice following EO treatment. Furthermore, the EOs regulated the expression of crucial biomarkers, including 5-hydroxytryptamine 1A receptor (5-HT1A), 5-HT2A, brain-derived neurotrophic factor, corticotropin-releasing factor, and tropomyosin-related kinase B. This study underscores the potential of EO inhalation as an adjunctive therapeutic strategy for depression treatment, provides novel insights into its molecular mechanisms, and supports further exploration of natural remedies in mental health care. Graphical
研究领域:
药理学
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神经科学
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精神病学
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芳香疗法
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天然产物
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分子生物学
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动物行为学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Bioengineering and Biotechnology
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2024
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IF 4.3
Jiang Fengqi,Zheng Qiuyan,Zhao Qingsong,Qi Zijuan,Wu Di,Li Wenzhong,Wu Xiaoke,Han Conghui
Research and development in the field of micro/nano-robots have made significant progress in the past, especially in the field of clinical medicine, where further research may lead to many revolutionary achievements. Through the research and experiment of microrobots, a controllable drug delivery system will be realized, which will solve many problems in drug treatment. In this work, we design and study the ability of magnetic-driven hydrogel microrobots to carry Lycorine hydrochloride (LH) to inhibit colorectal cancer (CRC) cells. We have successfully designed a magnetic field driven, biocompatible drug carrying hydrogel microsphere robot with Fe3O4 particles inside, which can achieve magnetic field response, and confirmed that it can transport drug through fluorescence microscope. We have successfully demonstrated the motion mode of hydrogel microrobots driven by a rotating external magnetic field. This driving method allows the microrobots to move in a precise and controllable manner, providing tremendous potential for their use in various applications. Finally, we selected drug LH and loaded it into the hydrogel microrobot for a series of experiments. LH significantly inhibited CRC cells proliferation in a dose- and time-dependent manner. LH inhibited the proliferation, mobility of CRC cells and induced apoptosis. This delivery system can significantly improve the therapeutic effect of drugs on tumors.
研究领域:
生物医学工程
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癌症研究
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药物递送系统
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纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
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2024
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IF 4.8
Yi-xin Dong,Teng-hui Li,Shan-shan Wang,Yan-hong Hu,Yi Liu,Fan Zhang,Tian-shi Sun,Chu-jun Zhang,Qing-hong Du,Wei-hong Li
Ethnopharmacological relevance Bu-Zhong-Yi-Qi Decoction(BZYQD) is a traditional formula commonly used in China, known for its effects in tonifying Qi and raising Yang. It can relieve symptoms of cognitive impairment such as forgetfulness and lack of concentration caused by qi deficiency, which is common in aging and debilitating. However, much of the current research on BZYQD has been focused on its impact on the digestive system, leaving its molecular mechanisms in improving cognitive function largely unexplored. Aim of the study Cognitive decline in the aging central nervous system is intrinsically linked to oxidative damage. This study aims to investigate the therapeutic mechanism of BZYQD in treating mild cognitive impairment caused by qi deficiency, particularly through repair of mitochondrial oxidative damage. Materials and methods A rat model of mild cognitive impairment (MCI) was established by administering reserpine subcutaneously for two weeks, followed by a two-week treatment with BZYQD/GBE. In vitro experiments were conducted to assess the effects of BZYQD on neuronal cells using a H 2 O 2 -induced oxidative damage model in PC12 cells. The open field test and the Morris water maze test evaluated the cognitive and learning memory abilities of the rats. HE staining and TEM were employed to observe morphological changes in the hippocampus and its mitochondria. Mitochondrial activity, ATP levels, and cellular viability were measured using assay kits. Protein expression in the SIRT3/MnSOD/OGG1 pathway was analyzed in tissues and cells through western blotting. Levels of 8-OH-dG in mitochondria extracted from tissues and cells were quantified using ELISA. Mitochondrial morphology in PC12 cells was visualized using Mito Red, and mitochondrial membrane potential was assessed using the JC-1 kit. Results BZYQD treatment significantly improved cognitive decline caused by reserpine in rats, as well as enhanced mitochondrial morphology and function in the hippocampus. Our findings indicate that BZYQD mitigates mtDNA oxidative damage in rats by modulating the SIRT3/MnSOD/OGG1 pathway. In PC12 cells, BZYQD reduced oxidative damage to mitochondria and mtDNA in H 2 O 2 -induced conditions and was associated with changes in the SIRT3/MnSOD/OGG1 pathway. Conclusion BZYQD effectively counteracts reserpine-induced mild cognitive impairment and ameliorates mitochondrial oxidative stress damage through the SIRT3/MnSOD/OGG1 pathway.
研究领域:
民族药理学
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神经科学
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药理学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
NEUROCHEMICAL RESEARCH
·
2022
·Vol. 47
·期 12
·3761-3776
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IF 4.414
He Junhui,Li Dongmei,Wei Jie,Wang Sheng,Chu Shifeng,Zhang Zhao,He Fei,Wei Dongmei,Li Yi,Xie Jiaxiu,Lai Kedao,Chen Naihong,Wei Guining
Depression has become an important disease threatening human health. In recent years, the efficacy of Traditional Chinese Medicine (TCM) in treating the disease has become increasingly prominent, so it is meaningful to find new antidepressant TCM. Mahonia fortune (Lindl.) Fedde is a primary drug in traditional formulas for the treatment of depression, and alkaloids are the main components of it. However, the detailed mechanism of Mahonia alkaloids (MA) on depression remains unclear. This study aimed to investigate the effect of MA on gap junction function in depression via the miR-205/Cx43 axis. The antidepressant effects of MA were observed by a rat model of reserpine-induced depression and a model of corticosterone (CORT)-induced astrocytes. The concentrations of neurotransmitters were measured by ELISA, the expression of Connexin 43 (Cx43) protein was measured by Immunohistochemistry and western-blot, brain derived neurotrophic factor (BDNF), cAMP-response element binding protein (CREB) proteins were measured by western-blot, the pathological changes of prefrontal cortex were observed by hematoxylin–eosin (H&E) staining. Luciferase reporter assay was performed to verify the binding of miR-205 and Cx43. The regulation effect of Cx43 on CREB was verified by interference experiment. Gap junction dysfunction was detected by fluorescent yellow staining. The results confirmed that MA remarkably decreased miR-205 expression and increased Cx43, BDNF, CREB expression in depression rat and CORT-induced astrocytes. In addition, after overexpression of miR-205 in vitro, the decreased expression of Cx43, BDNF and CREB could be reversed by MA. Moreover, after interfering with Cx43, the decreased expression of CREB and BDNF could be reversed by MA. Thus, MA may ameliorate depressive behavior through CREB/BDNF pathway regulated by miR-205/Cx43 axis. Graphical Abstract
研究领域:
药理学
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神经科学
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分子生物学
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中药
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Ling Jiang,Zhongqiang Zhu,Kun Han,Dongxu Chen,Lu Huang,Na Hu,Jiayi Du,Bowen Zhang,Yi Su,Tao Li,Xiangyi Ren,Xiang He
Fibromyalgia (FM), the most common causes of chronic musculoskeletal pain, is a complex syndrome with an indefinite etiology. Ferroptosis is a new type of cell death characterized by iron-dependent lipid peroxidation, affecting many chronic pain diseases. Here, we confirmed the role of ferroptosis in FM using a mouse model of reserpine induction. Then, we developed polyvinylpyrrolidone-assembled magnesium hexacyanoferrate nanocatalysts (MgHCF NCs) as a new type of ferroptosis inhibitor, for its efficacy in ferrous ions capture and antioxidation. Excellent in vivo FM-alleviation effect from MgHCF NCs was demonstrated. The FM-protective and antiferroptotic efficacy of MgHCF NCs at the transcriptional level were explored by whole-transcriptome analysis. Meanwhile, the remarkable antiferroptotic effect of MgHCF NCs was further validated in vitro ferroptosis-inducer experiments, which was firmly associated with the efficacy of MgHCF NCs on reducing oxidative stress, mitochondrial protection and correcting disordered polyunsaturated fatty acids (PUFAs) metabolism. The excellent antiferroptotic effect and biocompatibility render MgHCF NCs to be highly promising and clinically transformable agents for FM treatment
研究领域:
药理学
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纳米技术
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细胞生物学
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疼痛管理
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分子生物学
✓ 已验证
产品关联
DOI
高影响因子
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
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2025
·Vol. 39
·期 20
·e10103
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IF 1.7
Mengfei Shan,La Chen,Jun Wang,Luhong Wen
Rationale In electrospray ionization mass spectrometry (ESI-MS) systems, two critical challenges persist: (1) under-expanded supersonic jets at the atmospheric pressure interface (API) cause ion losses and reduced transmission efficiency; (2) residual solvents and charged droplets entering vacuum stages lead to contamination and elevated chemical noise, degrading analysis accuracy. Methods A dual-channel off-axis ion funnel with a deflection electrode (DC-OFIDE) was developed to address these challenges. This device integrates three core components: an ion drift channel (IDC), an ion funnel channel (IFC), and a deflection electrode. The IDC and IFC are separated by conjoined gaps. Ions within the gas stream emanating from the API are extracted from the IDC via a deflection field, while a retarding axial field prolongs ions' residence time, ensuring efficient transfer to the IFC. This DC-OFIDE features an enlarged entrance aperture (Φ18 mm) to accommodate a multi-capillary interface, enhancing compatibility with high-conductance sample introduction systems. Results Compared with the original conventional ion funnel (CIF), the DC-OFIDE achieved a threefold enhancement in caffeine ion intensity and a broader m/z transmission window. It demonstrated robust neutral and droplet suppression, maintaining 80% ion intensity even under tripled serum volume infused. In drug screening of hair samples, baseline noises in drug ion peaks were reduced by 36%–82%, with a quadrupled signal-to-noise ratio improvement observed for 6-monoacetylmorphine. Conclusions This DC-OFIDE significantly enhances ion transmission efficiency and chemical noise suppression in ESI-MS, establishing its potential for high-fidelity analysis of complex samples.
研究领域:
质谱分析
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分析化学
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离子光学
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生物医学分析
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药物筛选
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
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2025
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IF 6.7
Likai Xing,Daoxue Liu,Haohan Yu,Haibo Ding,Jie Sun,Guozhen Wang,Yuanyuan Deng,Wenya Chang,Zhuoying Xie
Electrospray is a widely recognized physical process and has various applications, such as in electrospinning, space propulsion, and mass spectrometry (MS). Particularly, electrospray emitters are integral to the exceptional performance of electrospray ionization mass spectrometry (ESI-MS). This study introduced a novel method for fabricating electrospray emitters using two-photon direct laser writing, resulting in the printing of nanosprayer arrays directly onto capillary tips. Utilizing a novel acrylic polyhedral oligomeric silsesquioxane photoresist, a multinozzle sprayer with micrometer scale channels was designed and printed on a capillary that is compatible with both frontend liquid chromatography and typical MS inlets. The 3D-printed sprayers demonstrated exceptional resistance to organic corrosion and high temperatures. For the nanosprayer arrays, an increase in the total electrospray current was achieved by augmenting the number of electrosprays, while maintaining a constant liquid flow rate and voltage. Notably, the eight-nozzle sprayers exhibited a 2.7-fold enhancement in sensitivity as compared with single-nozzle sprayers. This approach offers a versatile and high-sensitivity nanoelectrospray solution for ESI-MS.
研究领域:
分析化学
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材料科学
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质谱技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）