Scientific Reports
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2026
·
IF 4.9
Wang Lei,Hu Hanlu,Sun Xiaoxia,Jiao Ziwei,Zhang Hao,Luo Weikang,Huang Xiaode
In this study, essential oil was extracted from lavender using steam distillation, and its chemical components were identified by gas chromatography-mass spectrometry (GC–MS). The active ingredients of lavender essential oil (LEO) and their corresponding targets were obtained from the STP, SEA, and TCMSP databases. The antibacterial activities of the active components in LEO were investigated against Escherichia coli , Staphylococcus aureus , Bacillus subtilis , and Candida albicans . The antibacterial efficacy was evaluated by measuring the diameter of the inhibition zone and the minimum inhibitory concentration (MIC). The results showed that 156 common targets were identified by intersecting the component targets of LEO and the host targets related to antibacterial activity. Topological analysis was performed using Cytoscape software, and 21 potential core targets were finally obtained through two rounds of screening based on topological indexes. Gene Ontology (GO) enrichment analysis revealed that these targets were mainly involved in biological processes such as transcriptional regulation, while Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated their participation in 169 signaling pathways. Molecular docking results demonstrated that the core targets exhibited strong binding affinity with the four key components. In vitro antibacterial experiments verified that linalool, linalyl acetate, 1,8-cineole, and caryophyllene all showed favorable antibacterial activity. Combining network pharmacology, molecular docking, and in vitro antibacterial assays, this study clearly distinguished the direct antibacterial effect of lavender essential oil from its indirect anti-infectious role via host immune regulation. This work clarified the material basis for direct bacteriostasis and the auxiliary mechanism at the host level, providing an important reference for the potential application of lavender essential oil in the field of antibacterial treatment.
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ACS Nano
·
2025
·
IF 16
Yuai Li,Yunting Zhang,Jingwen Wang,Yujie Che,Tao Gong,Zhirong Zhang,Renhe Liu,Yao Fu
Selective autophagy relies on multivalent recognition by receptors like SQSTM1/p62 to form aggregates that cluster disperse organelles, undergoing liquid–liquid phase separation to facilitate their clearance and maintain cellular homeostasis. Inspired by this, we present the multivalent nanoparticle-based organelle targeting chimera (NanoTACOrg) to efficiently degrade organelles by flexibly clustering organelles for sequestration and facilitating targeted recruitment of autophagosomes. NanoTACOrg, assembled with a PLGA core, lysosomal escape modules, organelle-targeting modules, and LC3B binding modules, is programmed to selectively degrade various organelles, including mitochondria, endoplasmic reticulum, and Golgi apparatus. After endocytosis and lysosomal escape, NanoTACOrg targets subcellular compartments and mimics p62 aggregate-driven organelle clustering and degradation, without exhibiting the “hook effect”. Specifically, NanoTACMito-mediated mitochondrial degradation disrupts oxidative phosphorylation (OXPHOS) while enhancing compensatory glycolysis, thus sensitizing tumor cells to the glucose transporter 1 (GLUT1) inhibitor BAY-876. BAY-876 loaded NanoTACMito potently inhibits tumor growth, recurrence, and metastasis, demonstrating superior therapeutic efficacy by simultaneously targeting OXPHOS and glycolysis. These findings highlight the potential of NanoTACOrg as a versatile and effective platform for cancer therapy, particularly through organelle-specific degradation and metabolic reprogramming.
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ANALYTICA CHIMICA ACTA
·
2023
·
IF 6.2
Wei Liu,Yan Wang,Tengfei Wang,Liwen Wang,Sheng Hu,Dating Tian
Abnormal viscosity and excessive superoxide anion (O 2 •- ) levels in living cells often cause a series of biological dysfunction and oxidative damage. However, a great challenge remains in quickly and conveniently detecting the viscosity and O 2 •- levels in living cells. Herein, we fabricated a versatile aggregation-induced emission (AIE) probe with mitochondria targeting, DTPB, for dual-imaging of viscosity and O 2 •- level in living cells with two different channels. The obtained DTPB contained a diphenyl phosphinic acid unit responsive to O 2 •- , a unit with twisted intramolecular charge trans (TICT) function responsive to viscosity, and a pyridine cation unit with mitochondria targeting. The results showed that DTPB exhibited a remarkable response to viscosity with a near-infrared emission peak at 671 nm and was highly sensitive to O 2 •- levels with an emission peak at 587 nm. The dual-channel probe has great application prospects in the visual diagnosis of cancer and related diseases.
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Advanced Healthcare Materials
·
2023
·Vol. 12
·期 18
·2300338
·
IF 10
Junwu Wei,Yuan Liu,Yingxin Li,Zhanlin Zhang,Jie Meng,Shuang Xie,Xiaohong Li
Cancer phototherapy experiences limitations in tissue diffusion and cell internalization of phototherapeutic agents and dose-dependent side effects. Herein, Janus pyroelectric nanoparticles (NPs) are designed to generate self-powered motion and built-in electric fields to overcome the delivery barriers. Polydopamine (PDA) layers are partially coated on tetragonal BaTiO 3 (tBT) NPs to prepare Janus tBT@PDA, and Au NPs are deposited on the PDA caps to obtain Janus tBT@PDA-Au NPs. Near-infrared (NIR) illumination of tBT@PDA-Au builds in situ pyroelectric potentials on NPs, which selectively affect the membrane potential of tumor cells rather than normal cells to enhance tumor cell internalization and produce reactive oxygen species (ROS) for pyroelectric dynamic therapy (PEDT). The asymmetric photothermal effect of the Janus NPs creates thermophoretic force to propel NP motion, which enhances tumor diffusion and cellular uptake of NPs and boosts cytotoxicity and intracellular ROS levels. The inoculation of Au NPs increases the photothermal effect, exhibits larger motion velocities, produces higher pyroelectric potentials, and elevates cellular uptake rates, resulting in significant induction of tumor cell apoptosis, suppression of tumor growth, and extension of animal survival. Thus, the concise design of tBT@PDA-Au/NIR treatment has achieved thermophoretic motion-promoted tissue diffusion, built-in electric field-enhanced cell internalization, and photothermal/PEDT-synergized antitumor efficacy.
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CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Ping Guan,Zhaoxu Meng,Xinran Liu,Jiaxiang Mu,Zichun Gao,Xinrong Yu,He Lian
The complex, invasive, and metastatic nature of malignant tumors make the current treatment of patients challenging in advanced stages. The present study proposes a multi-effect synergistic biomimetic mineralization nanosystem ( [email protected] 4 ) to regulate tumor cell metabolism and relevant biological behaviors through glycolysis-oxidative stress-autophagy process. Bovine serum albumin complexed with lonidamine served as a template to induce the formation of iron phosphate mineralization in situ . After intratumoral administration, the released lonidamine inhibited hexokinase II and down-regulated the level of glycolysis, cutting off the energy and biosynthesis supply source for tumor growth. Ferric ions dissociated from biomineralization in acidic microenvironment converted reduced glutathione to oxidized glutathione, elevated oxidative stress and induced ferroptosis through chemokinetic reactions. The dissociated phosphate ions function as an autophagy inhibitor to block the protective autophagy cased by energy deprivation for efficacy enhancement. The up-regulation of Nrf2 and LC3 proteins, the down-regulation of HK2 and GPX4 proteins, and the increase of ROS level in tumors all confirmed this conclusion. Notably, the nanosystem also down-regulated the release of inflammatory factors (IL-6, IL-8) and metastasis-related proteins (N-Cadherine, Vimentin) simultaneously, effectively preventing distant lung metastasis of tumor. In summary, this biomineralization-based nanosystem exhibited powerful antitumor effects both in vitro and in vivo and provides a new perspective for addressing the dilemma of malignant tumor treatment.
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CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Zhanlin Zhang,Tian Xia,Pan Ran,Junwu Wei,Jie Meng,Guiyuan Zhang,Xiaohong Li
Cancer photothermal (PTT) and photodynamic therapies (PDT) have aroused tremendous attention with high spatial specificity, but they experience the challenges from the inefficient tumor accumulation and penetration of photosensitizers and dose-dependent side effects. Herein, persistent luminescence (PL)-activated nanomotors are developed with the integration of PL-illuminated PTT and PDT to overcome these limitations. PL nanodots of ZnGa 2 O 4 :Cr 3+ (ZGC) were deposited on mesoporous silica nanoparticles (NPs), followed by conjugation of silicon phthalocyanine as a photosensitizer and capping with polydopamine (PDA) to fabricate Janus [email protected] NPs. ZGC nanodots on NPs are activated by external illumination to generate PL as an internal light source to excite PDA and phthalocyanine for persistent productions of heat and reactive oxygen species (ROS), avoiding high heat stress and ROS levels in the conventional intermittent phototherapy. In addition, the persistent heat gradients around the Janus NPs create thermophoretic force to drive their motions and enhance their cellular uptake, and the PL-illuminated PDT continuously produces intracellular ROS to damage tumor cells. Light activation of [email protected] obviously promotes accumulation and deep penetration of NPs into tumors and produces mild thermal and ROS levels with well distribution across the tumors. The self-propelled tumor distribution and the combined PDT/PTT treatment led to full inhibition of tumor growth and significant extension of animal survival, and there is no systemic toxicity and function fluctuation of the major organs. Thus, this study demonstrates a concise strategy to generate PL-activated motion for deep penetration and produce PL-illuminated PDT/PTT for synergistic tumor treatment.
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MICROBIAL ECOLOGY
·
2023
·
IF 3.6
Ma Wenwen,Lin Lu,Peng Qiannan
Terrestrial microbial consortia were reported to play fundamental roles in the global carbon cycle and renewable energy production through the breakdown of complex organic carbon. However, we have a poor understanding of how biotic/abiotic factors combine to influence consortia assembly and lignocellulose degradation in aquatic ecosystems. In this study, we used 96 in situ lignocellulose enriched, coastal intertidal zone-derived bacterial consortia as the initial inoculating consortia and developed 384 cultured consortia under different lignocellulose substrates (aspen, pine, rice straw, and purified Norway spruce lignin) with gradients of salinity and temperature. As coastal consortia, salinity was the strongest driver for assembly, followed by Norway spruce lignin, temperature, and aspen. Moreover, a conceptual model was proposed to demonstrate different succession dynamics between consortia under herbaceous and woody lignocelluloses. The succession of consortium under Norway spruce lignin is greatly related with abiotic factors, while its substrate degradation is mostly correlated with biotic factors. A discrepant pattern was observed in the consortium under rice straw. Finally, we developed four groups of versatile, yet specific consortia. Our study not only reveals that coastal intertidal wetlands are important natural resources to enrich lignocellulolytic degrading consortia but also provides insights into the succession and ecological function of coastal consortium.
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APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2023
·Vol. 107
·期 5
·1551-1562
·
IF 5
Ye Jianbin,Li Jianqing,Wang Qiuhui,Wang Xiangjiang,Wang Shouan,Wang Honglin,Xu Jia
Abstract
In this study, loquat extract was selected as a promising substrate for bacterial cellulose (BC) production. A new BC-producing bacterial strain was isolated from residual loquat and identified as Komagataeibacter rhaeticus . BC production with different carbon sources and with loquat extract was investigated. Among all tested carbon sources, glucose was demonstrated to be the best substrate for BC production by K. rhaeticus , with up to 7.89 g/L dry BC obtained under the optimal initial pH (5.5) and temperature (28 °C) with 10 days of fermentation. The total sugar and individual sugars were investigated in different loquat extracts, in which fructose, glucose, and sucrose were the three main sugars. When loquat extract was prepared with a solid‒liquid (S-L) ratio of 2:1, the concentrations of glucose, fructose, and sucrose were 7.91 g/L, 9.31 g/L, and 2.84 g/L, respectively. The BC production obtained from loquat extract was higher than that of other carbon sources except glucose, and 6.69 g/L dry BC was obtained from loquat extract with an S-L ratio of 2:1. After BC production, all sugars substantially decreased, with the utilization of glucose, fructose, and sucrose reaching 93.9%, 87.9%, and 100%, respectively. These results suggested that the different sugars in loquat extract were all carbon sources participating in BC production by K. rhaeticus . Structural and physicochemical properties were investigated by SEM, TGA, XRD, and FT-IR spectroscopy. The results showed that the structural, chemical group, and water holding capacity of BC obtained from loquat extract were similar to those of BC obtained from glucose, but the crystallinity and thermal stability of BC were higher than those of BC from mannose and lactose but lower than those of BC from glucose and fructose. Key points A new BC-producing strain was isolated and identified as Komagataeibacter rhaeticus.
Loquat extract is an alternative substrate for BC production.
The BC obtained from loquat extract owns advanced physicochemical properties . Graphical Abstract
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ACS Applied Materials & Interfaces
·
2023
·
IF 9.5
Haifeng Lu,Qi Lu,Hongwu Sun,Zhongkun Wang,Xiang Shi,Yuling Ding,Xiang Ran,Jing Pei,Yubo Pan,Qunlin Zhang
Intracellular reactive oxygen species (ROS) are closely associated with cancer cell types. Therefore, ROS-based pattern recognition is a promising strategy for precise diagnosis of cancer, but such a possibility has never been reported yet. Herein, we proposed an ROS-responsive fluorescent sensor array based on pH-controlled histidine-templated gold nanoclusters (AuNCs@His) to distinguish cancer cell types and their proliferation states. In this strategy, three types of AuNCs@His with diverse fluorescence profiles were first synthesized by only adjusting the pH value. Upon the addition of various ROS, fluorescence quenching of three types of AuNCs@His occurred with different degrees, thereby forming unique optical “fingerprints”, which were well-clustered into several separated groups without overlap by principal component analysis (PCA). The sensing mechanism was attributable to the oxidation of AuNCs@His by ROS, as revealed by X-ray photoemission spectroscopy, Fourier transform infrared spectroscopy, 1H nuclear magnetic resonance spectroscopy, and electrospray ionization mass spectrometry. Based on the ROS-responsive sensing pattern, cancer cell types were successfully differentiated via PCA with 100% accuracy. Additionally, the proposed sensor array exhibited excellent performance in distinguishing the proliferation states of cancer cells, which was supported by the results of the Ki-67 immunohistochemistry assay. Overall, the ROS-responsive fluorescent sensor array can serve as a promising tool for precise diagnosis of cancer, indicating great potential for clinical application.
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ACS Nano
·
2023
·
IF 17.1
Zhanlin Zhang,Hui Yan,Wenxiong Cao,Shuang Xie,Pan Ran,Kun Wei,Xiaohong Li
Cancer phototherapy indicates advantages in ease of manipulation, negligible drug resistance, and spatiotemporal control but is confronted with challenges in tumor cell accessibility and intermittent light excitation. Herein, we propose a strategy with persistent luminescence (PL)-excited photothermal therapy (PTT), concurrent thermophoresis-propelled motion, and PL-triggered NO release, where PL emission is chargeable by ultrasonication for readily applicable to deep tumors. Mechanoluminescent (ML) nanodots of SrAl2O4:Eu2+ (SAOE) and PL nanodots of ZnGa2O4:Cr3+ (ZGC) were deposited on mesoporous silicates to obtain mSZ nanoparticles (NPs), followed by partially coating with polydopamine (PDA) caps and loading NO donors to prepare Janus mSZ@PDA-NO NPs. The ML emission bands of SAOE nanodots overlap with the excitation band of ZGC, and the persistent near-infrared (NIR) emission could be repeatedly activated by ultrasonication. The PL emission acts as an internal NIR source to produce a thermophoretic force and NO gas propellers to drive the motion of Janus NPs. Compared with the commonly used intermittent NIR illumination at both 660 and 808 nm, the persistent motion of ultrasound-activated NPs enhances cellular uptake and long-lasting PTT and intracellular NO levels to combat tumor cells without the use of any chemotherapeutic drugs. The ultrasound-activated persistent motion promotes intratumoral accumulation and tumor distribution of PTT/NO therapeutics and exhibits significantly higher tumor growth inhibition, longer animal survival, and larger intratumoral NO levels than those who experience external NIR illumination. Thus, this study demonstrates a strategy to activate PL emissions and construct PL-excited nanomotors for phototherapy in deep tissues.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2023
·
IF 4.4
Wei Liu,Tengfei Wang,Liwen Wang,Yan Wang,Sheng Hu,Dating Tian
The development of fluorescent probes capable of detecting abnormal changes in cellular mitochondrial viscosity is of great significance, as these changes have been connected to many diseases. In this study, the conventional tetraphenylethylene (TPE) molecule was modified to fabricate a novel near-infrared fluorescent, TTPB, which was then used to measure the mitochondrial viscosity. Due to the introduction of TPE and pyridine groups, TTPB had an AIE effect and mitochondrial targeting function. Meanwhile, TTPB was extremely sensitive to variations in viscosity for the twisted intramolecular charge transfer (TICT) phenomenon. The logarithm of fluorescence intensity (log I 620 ) of the probe demonstrated an excellent linear connection with the logarithm of viscosity (log η ) in the viscosity range of 1.2 ∼ 956.0 cP, indicating the probe could quantitatively detect viscosity. Moreover, TTPB was able to visually track autophagy in addition to detecting the mitochondrial viscosity in the inflammatory cell model. These results showed that the probe was anticipated to be employed for the early diagnosis of related diseases.
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3 Biotech
·
2022
·Vol. 12
·期 9
·1-15
·
IF 2.893
Weng Chunyue,Mi Zheyan,Li Meijing,Qin Haibin,Hu Zhongce,Liu Zhiqiang,Zheng Yuguo,Wang Yuanshan
To improve S-Adenosyl-L-methionine (a compound with important physiological functions, SAM) production, atmospheric and room temperature plasma and ultraviolet-LiCl mutagenesis were carried out with Saccharomyces cerevisiae strain ZY 1–5. The mutants were screened with ethionine, L-methionine, nystatin and cordycepin as screening agents. Adaptive evolution of a positive mutant UV6-69 was further performed by droplet microfluidics cultivation with ethionine as screening pressure. After adaptation, mutant T11-1 was obtained. Its SAM titer in shake flask fermentation reached 1.31 g/L, which was 191% higher than that of strain ZY 1–5. Under optimal conditions, the SAM titer and biomass of mutant T11-1 in 5 L bioreactor reached 10.72 g/L and 105.9 g dcw/L (142.86% and 34.22% higher than those of strain ZY 1–5), respectively. Comparative transcriptome analysis between strain ZY 1–5 and mutant T11-1 revealed the enhancements in TCA cycle and gluconeogenesis/glycolysis pathways as well as the inhibitions in serine and ergosterol synthesis of mutant T11-1. The elevated SAM synthesis of mutant T11-1 may attribute to the above changes. Taken together, this study is helpful for industrial production of SAM.
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