JOURNAL OF FOOD COMPOSITION AND ANALYSIS
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2025
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IF 4.6
Siming Nie,Xiaofei Xie,Mengying Zhang,Mengfei Tian,Qi Liang,Chunjian Zhao,Chunying Li
In this study, a series of natural low-melting mixture solvents (NLoMMSs) were prepared using choline chloride as the hydrogen bond acceptor and various compounds as the hydrogen bond donor for the extraction of principal phytochemicals, namely eleutheroside B, eleutheroside E, rutin, hyperoside, and quercitrin from E. senticosus . The optimal NLoMMS was determined to comprise lactic acid to choline chloride with a molar ratio of 3:1, accompanied by a water content of 30 %. In comparison to other extraction solvents, the prepared NLoMMS serve as a green and sustainable extraction medium, which shows high extraction yield for phytochemicals in E. senticosus . The optimal extraction conditions were determined to be: an extraction time of 20 min, an extraction pressure of −0.07 MPa, and a liquid-solid ratio of 20 mL/g. The surface morphology and functional groups of phytochemicals before and after extracting by NLoMMS were systematically characterized. It was observed that NLoMMS could more effectively destroy the cell wall, which was conducive to the efficient extraction of phytochemicals. Furthermore, the extraction mechanism of phytochemicals by NLoMMS was explored by molecular dynamics simulation. The results indicate that van der Waals forces, hydrogen bonding, and electrostatic interactions are the primary interaction forces between NLoMMS and phytochemicals.
研究领域:
植物化学
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绿色化学
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提取技术
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分子模拟
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天然产物化学
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产品关联
DOI
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
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2023
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IF 5.4
Yan Cao,Jinpeng Lv,Yan Tan,Ruolin Chen,Xiaoxue Jiang,Duo Meng,Kun Zou,Min Pan,Liming Tang
Ethnopharmacological relevance Tribuloside, a natural flavonoid extracted from Chinese medicine Tribulus terrestris L., has shown potent efficacy in treating various diseases. In China, the fruits of Tribulus terrestris L. have long been utilized for relieving headache, dizziness , itchiness, and vitiligo . Water-based extract derived from Tribulus terrestris L. can enhance melanogenesis in mouse hair follicle melanocytes by elevating the expression of α-melanocyte stimulating hormone (α-MSH) and melanocortin-1 recepter (MC-1R). Nevertheless, there is a lack of information regarding the impact of tribuloside on pigmentation in both laboratory settings and living organisms. Aim of the study The present research aimed to examine the impact of tribuloside on pigmentation, and delve into the underlying mechanism. Materials and methods Following the administration of tribuloside in human epidermal melanocytes (HEMCs), we utilized microplate reader, Masson-Fontana ammoniacal silver stain, transmission electron microscopy (TEM) and scanning electron microscopy (SEM) to measure melanin contents, dendrite lengths, melanosome counts; L-DOPA oxidation assay to indicate tyrosinase activity, Western blotting to evaluate the expression of melanogenic and associated phosphodiesterase (PDE)/cyclic adenosine monophosphate (cAMP)/cyclic-AMP dependent protein kinase A (PKA) pathway proteins. A PDE-Glo assay to verify the inhibitory effect of tribuloside on PDE was also conducted. Additionally, we examined the impact of tribuloside on the pigmentation in both zebrafish model and human skin samples. Results Tribuloside had a notable impact on the production of melanin in melanocytes, zebrafish, and human skin samples. These functions might be attributed to the inhibitory effect of tribuloside on PDE, which could increase the intracellular level of cAMP to stimulate the phosphorylation of cAMP-response element binding (CREB). Once activated, it induced microphthalmia-associated transcription factor (MITF) expression and increased the expression of tyrosinase, Rab27a and cell division cycle protein 42 (Cdc42), ultimately facilitating melanogenesis, melanocyte dendricity, and melanin transport. Conclusion Tribuloside acts on the PDE/cAMP/PKA pathway to enhance melanogenesis, melanocyte dendricity, and melanosome transport; meanwhile, tribuloside does not have any toxic effects on cells and may be introduced into clinical prescriptions to promote pigmentation.
研究领域:
药理学
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细胞生物学
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分子生物学
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民族药理学
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产品关联
DOI
PubMed
高影响因子
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