AQUACULTURE RESEARCH
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2016
·Vol. 48
·期 5
·2278-2290
·
IF 1.606
Jing Qian,Chunhua Ren,Ting Chen,Jianjun Xia,Zonghe Yu,Yan Gao,Chaoqun Hu
The broad-spectrum protease inhibitor alpha-2-macroglobulin (α 2 M) is widely found in vertebrates and invertebrates. However, no research regarding α 2 M has been described in sea cucumbers to date. In this study, the first sea cucumber α 2 M named Stm α 2 M was identified from Stichopus monotuberculatus , a tropical species with high edible and economic values in Southern China. The Stm α 2 M cDNA is 5276 bp in length, containing a 5′-untranslated region (UTR) of 279 bp, a 3′-UTR of 344 bp and an open reading frame of 4653 bp that encodes a protein of 1550 amino acids. The Stmα 2 M possesses all the three characteristics of known α 2 M proteins, including the bait region domain, thioester domain and receptor-binding domain. Higher levels of mRNA expression were noticed in intestine, respiratory tree, coelomocytes and tentacle of S. monotuberculatus . Transcriptional expression of Stm α 2 M showed the strongest response to lipopolysaccharides (LPS, 8.19-fold up-regulation) after 6 h post-challenge in coelomocytes. In addition, the α 2 M activity was detected in S. monotuberculatus and protein activity of α 2 M increased remarkably at 6 h after LPS stimulation. As a whole, this study indicated that Stmα 2 M is an important immune-related molecule, and the study on functions of Stmα 2 M may provide some new and valuable ideas for preventing and controlling diseases of cultured sea cucumbers.
研究领域:
免疫学
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分子生物学
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海洋生物学
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DOI
高影响因子
FOOD CHEMISTRY
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2025
·
IF 9.8
Jinghe Sun,Weiyun Zheng,Xiaoyan Wang,Yimeng Li,Jingfeng Yang
The freshness in A. japonicus after thymol treatment was tested. The results showed that sensory scores in thymol group significantly increased 14.83 and 21.67 compared to the Frozen and BK groups after storage. Texture parameters showed that hardness, springiness, and chewiness of body wall treated with thymol were 2.56, 1.21, and 2.02 folds that of the BK group, respectively. Volatile flavor results revealed 38 flavor substances and 33 volatile organic compounds in the body wall. Aldehydes, alcohols, heterocycles, and alkane were the key sources to odor components of A. japonicus , including nonanal, hexanal, 1-octanol, 1-hexanol, d -limonene, and 2-methylbutyl acetate. Moreover, heptenoic acid, dimethyl disulfide, and dimethylamine, as distinct volatile flavor substances, were the main contributors of fishy, malodorous, and spicy smells of autolysis A. japonicus . This study guides the potential use of thymol in sea cucumber preservation and seeks the spoiled flavor markers for non-destructive rapid testing technology development.
研究领域:
食品科学
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海洋生物学
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DOI
PubMed
高影响因子
Current Research in Microbial Sciences
·
2025
·
IF 5.8
Zhi Wu,Yina Shao,Shanshan Zhang,Chenghua Li
Vibrio splendidus AJ01 is the primary pathogen of Apostichopus japonicus , resulting in huge economic losses. In the present study, we found that 5-iodoindole exhibits a remarkable protective effect on A. japonicus , the survival rate of which can be improved to 93.75%. To explore the impact of 5-iodoindole on V. splendidus AJ01, bacterial growth and virulence factors were determined. Results showed that 5-iodoindole significantly decreased swimming motility, hemolytic activity and protease activity but had minimal effect on the overall growth of the bacterium. Furthermore, a differentially expressed protein was downregulated and identified as elongation factor thermo unstable protein (EF-Tu) via SDS-PAGE combined with LC-MS/MS. Moreover, 5-iodoindole had impact on a large number of genes at RNA level, such as genes related to two-component system, ABC transporters, flagella, and chemotaxis. In addition, 20 µM 5-iodoindole could upregulated the mRNA level of immune related genes in A. japonicus , including IL-17, VEGF and MMP1. Our results indicated 5-iodoindole protect A. japonicus through a two-pronged approach: not only inhibiting bacterial virulence but also enhancing the host's immunity. This not only addresses the limitations of traditional indole but also provides a green alternative to antibiotics for protecting A. japonicus from infections caused by V. splendidus AJ01.
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PubMed
高影响因子
Marine Drugs
·
2019
·Vol. 17
·期 4
·195
·
IF 3.772
Ruowei Guan,Yuan Peng,Lutan Zhou,Wenqi Zheng,Xixi Liu,Pin Wang,Qingxia Yuan,Na Gao,Longyan Zhao,Jinhua Zhao
Apostichopus japonicusis one of the most economically important species in sea cucumber aquaculture in China. Fucosylated glycosaminoglycan fromA. japonicus(AjFG) has shown multiple pharmacological activities. However, results from studies on the structure of AjFG are still controversial. In this study, the deaminative depolymerization method that is glycosidic bond-selective was used to prepare the depolymerized products from AjFG (dAjFG), and then a series of purified oligosaccharide fragments such as tri-, hexa-, nona-, and dodecasaccharides were obtained from dAjFG by gel permeation chromatography. The 1D/2D NMR and ESI-MS spectrometry analyses showed that these oligosaccharides had the structural formula ofl-FucS-α1,3-d-GlcA-β1,3-{d-GalNAc4S6S-β1,4-[l-FucS-α1,3-]d-GlcA-β1,3-}n-d-anTal-diol4S6S(n= 0, 1, 2, 3; FucS represents Fuc2S4S, Fuc3S4S, or Fuc4S). Thus, the unambiguous structure of native AjFG can be rationally deduced: it had the backbone of {-4-d-GlcA-β1,3-d-GalNAc4S6S-β1-}n, which is similar to chondroitin sulfate E, and eachd-GlcA residue in the backbone was branched with al-FucS monosaccharide atO-3. Bioactivity assays confirmed that dAjFG and nonasaccharides and dodecasaccharides from AjFG had potent anticoagulant activity by intrinsic FXase inhibition while avoiding side effects such as FXII activation and platelet aggregation.Keywords:Apostichopus japonicus;fucosylated glycosaminoglycan;oligosaccharide;unambiguous structure;anti-Fxase
研究领域:
海洋生物学
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药理学
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生物化学
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PubMed
高影响因子
MARINE BIOTECHNOLOGY
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2025
·Vol. 27
·期 2
·1-18
·
IF 2.8
Li Xue,Meng Fanying,Sun Tong,Hao Zezhuang,Wang Yihua,Jiang Yunfei,Wang Ye,Li Yong,Ding Yuling
This article aims to reveal the optimal peptide segment with antioxidant activity from Dalian Stichopus japonicus , investigate its anti-melanogenesis effect, and elucidate its mechanisms of action both in vitro and in vivo. The best antioxidant alcalase hydrolysates, identified by the previous screening of proteases, was isolated by ultrafiltration; it was found that the components with a molecular weight of ≤ 3 kDa exhibited the best activity. The chemical components were characterized using LC–MS/MS. Through molecular docking, GPIGF was identified as the peptide segment with the best antioxidant and melanogenesis-inhibitory activity. A search in the NCBI database revealed that GPIGF is a newly obtained natural oligopeptide. Further experiments with synthesized GPIGF in vitro showed that it effectively reduces cell apoptosis and damage, and inhibits the expression of melanin-related genes, including tyrosinase (TYR), and associated proteins TRP-1, TRP-2, and MITF. In vivo experiments with the zebrafish model demonstrated that GPIGF significantly inhibits AAPH-induced apoptosis in zebrafish larvae, reduces the production of ROS, and suppresses melanin generation on the skin surface without exhibiting embryotoxicity. This study provides a research foundation for the development of antioxidants from Dalian Stichopus japonicus , which could serve as natural whitening and anti-aging agents, supporting its integrated utilization and development.
研究领域:
生物化学
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药理学
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海洋生物学
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PubMed
高影响因子
JOURNAL OF THE WORLD AQUACULTURE SOCIETY
·
2016
·Vol. 48
·期 2
·360-373
·
IF 0.665
Jing Qian,Chunhua Ren,Jianjun Xia,Zonghe Yu,Chaoqun Hu
Alpha-2-macroglobulin (A 2 M) is essential for hosts defending against pathogen infections. In this study, two distinct A 2 Ms were identified from sea cucumbers Stichopus monotuberculatus and Holothuria atra , which belong to different genera in the Phylum Echinodermata, and named StmA 2 M and HaA 2 M, respectively. Structural analysis of StmA 2 M and HaA 2 M revealed that all contain the bait region domain, thioester domain, and receptor-binding domain. Phylogenetic analysis indicated that StmA 2 M and HaA 2 M all belong to the echinoderm A 2 Ms. The cleavage site RXXR and the catalytic histidine residue for complement component C3 were found in StmA 2 M and HaA 2 M. Compared with human A 2 M, the three-dimensional structures of StmA 2 M and HaA 2 M were more similar to that of common carp A 2 M. Both StmA 2 M and HaA 2 M showed the strongest response at transcriptional levels to lipopolysaccharide (LPS) at 12 h and polyriboinosinic polyribocytidylic acid [Poly (I: C)] at 24 h, respectively. Activity of StmA 2 M showed the strongest response to Poly (I: C) at 6 h, while the strongest change of HaA 2 M activity was induced by LPS at 24 h. The differential mRNA expression and enzyme activity levels suggested that A 2 M in sea cucumbers might be involved in different immune responses to LPS or Poly (I: C) challenge.
研究领域:
海洋生物学
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免疫学
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分子生物学
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基因组学
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高影响因子
Food & Function
·
2021
·Vol. 12
·期 12
·5551-5562
·
IF 5.396
Mengjie Ma,Han Wang,Yongkai Yuan,Yuanhong Wang,Shuang Yang,Zhihua Lv
The purpose of this study was to investigate the absorption and transport of glycosaminoglycan from Apostichopus japonicus (AHG) and its depolymerized derivatives (DAHG-1, DAHG-2, DAHG-3). The AHG and depolymerized AHGs (DAHGs) were characterized by high-performance gel permeation chromatography (HPGPC), Raman spectroscopy and atomic force microscopy (AFM). The results showed that there was no significant difference of an AHG primary structure and functional groups during the depolymerization. Meanwhile, AFM observation showed that AHG and DAHGs possessed linear structures. In this study, a rapid and sensitive liquid chromatographic post-column derivatization method was used to investigate the absorption of AHG and DAHGs with the Caco-2 cell model, the M cell model and the intestinal recirculating perfusion. It was found that AHG and DAHGs can be absorbed in the intestine, and their transport involved endocytosis.
研究领域:
生物化学
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药学
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营养学
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PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Benyou Zhang,Likun Ren,Yilin Lu,Zongji Wang,Guilin Zhang,Zhongwei Liu,Yuping Wang,Fenglan Zhu,Xuepeng Li,Yang Jiang,Zhiteng Liu,Fangchao Cui,Dangfeng Wang,Tingting Li,Jianrong Li
The sea cucumber collagen contains a high content of hydrophobic amino acids, which play essential roles in various bioactivities. A total of 2647 unknown active peptide fragments (2–20 amino acids) were obtained via virtual enzymolysis from 16 known collagen sequences in Apostichopus japonicus . Then, the novel bifunctional hexapeptide (DCDPRL, 717.788 Da) with hypoglycemic and antioxidant activities was identified via molecular docking and pharmacokinetics. DCDPRL revealed strong radical scavenging capacity in vitro with IC 50 values for DPPH, ABTS, OH, and O 2 − of 206.12 μM, 186.06 μM, 1.52 mM, and 247.71 μM, respectively. In addition, DCDPRL (250 μM) effectively reduced blood glucose levels and oxidative damage in zebrafish larvae through glucagon-like peptide-1 receptor (GLP-1R), glucose-dependent insulinotropic peptide receptor (GIPR) and Kelch-like ECH-associated protein 1 (Keap1) targets. Furthermore, the structure–activity relationship analysis revealed that sulfur-containing cysteine, hydrophobic amino acids (proline and leucine), and repeated acidic aspartic acid were essential for bifunctional activities.
研究领域:
海洋生物技术
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生物化学
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药理学
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计算生物学
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食品科学
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结构生物学
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PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Jing Ye,Linjing Zheng,Weipeng Pan,Yayan Huang,Na Zhang,Dongda Yang,Yucheng Yang,Bingde Zheng,Xueqin Zhang,Meitian Xiao
Polysaccharides from sea cucumbers are known for their biological activities, but little is known about those from sea cucumber viscera. The present study isolated a sulfated polysaccharide (SCVP-2) from the viscera of Apostichopus japonicas , which had a molecular weight of 209.1 kDa. SCVP-2 comprised 66.3 % total sugars, 2.1 % uronic acid, 4.5 % proteins, and 25.5 % sulfate groups, containing glucosamine, galactosamine, glucose, galactose, and fucose. FT-IR and NMR analyses identified SCVP-2 as a fucoidan sulfate with sulfation patterns of the fucose branches as Fuc2S, Fuc4S, and Fuc0S. SEM and AFM analyses showed irregular clusters and linear conformations. SCVP-2 demonstrated strong anti-inflammatory properties both in vitro and in vivo . In lipopolysaccharide (LPS)-induced inflammation in macrophage RAW264.7 cells, SCVP-2 significantly reduced nitric oxide (NO) and cytokine secretion (IL-1β, IL-6, TNF-α). Additionally, it downregulated the expression of these cytokine genes. Furthermore, the anti-inflammatory mechanism of SCVP-2 was related to the inhibition of the MAPKs and NF-κB pathways. SCVP-2's anti-inflammatory capacity was confirmed in acute inflammation models, including xylene-induced ear swelling and acetic acid-induced peritoneal capillary permeability, and in high-fat diet-induced systemic low-grade chronic inflammation. In conclusion, SCVP-2 exhibits significant anti-inflammatory activity, suggesting its potential for development as a functional food ingredient or therapeutic agent for inflammation-related diseases.
研究领域:
海洋生物学
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药理学
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免疫学
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PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
·
IF 7.7
Yangxi Xiang,Yue Shi,Lianlian Sun,Weikang Liang,Kaiyu Chen,Chenghua Li
Pattern recognition receptors (PRRs) mediate the innate immune responses and play a crucial role in host defense against pathogen infections. Apextrin C-terminal (ApeC)-containing proteins (ACPs), a newly discovered class of PRRs specific to invertebrates, recognize pathogens through their ApeC domain as intracellular or extracellular effectors. However, the other immunological functions of ACPs remain unclear. In this study, a membrane-localized ACP receptor was identified in the sea cucumber Apostichopus japonicus (denoted as AjACP1). The ApeC domain of AjACP1, which was located outside of its cell membrane, exhibited the capability to recognize and aggregate Vibrio splendidus . AjACP1 was upregulated upon V. splendidus infection, internalizing into the cytoplasm of coelomocytes. AjACP1 overexpression enhanced the phagocytic activity of coelomocytes against V. splendidus , while knockdown of AjACP1 by RNA interfere inhibited coelomocyte endocytosis. Inhibitor experiments indicated that AjACP1 regulated coelomocyte phagocytosis through the actin-dependent endocytic signaling pathway. Further investigation revealed that AjACP1 interacted with the subunit of the actin-related protein 2/3 complex ARPC2, promoting F-actin polymerization and cytoskeletal rearrangement and thereby affecting the coelomocyte phagocytosis of V. splendidus via the actin-dependent endocytic signaling pathway. As a novel membrane PRR, AjACP1 mediates the recognition and phagocytic activity of coelomocytes against V. splendidus through the AjACP1-ARPC2-F-actin polymerization and cytoskeletal rearrangement pathway.
研究领域:
免疫学
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海洋生物学
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分子生物学
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DOI
高影响因子
Foods
·
2024
·Vol. 13
·期 6
·963
·
IF 4.7
Haoran Song,Chen Song,Chunhong Yan,Jingfeng Yang,Shuang Song
To prevent alcoholic liver disease, the addition of bioactive substances to the alcoholic drink Baijiu has been considered a feasible option. In the present study, the hepatoprotective effects of a sea cucumber sulfated polysaccharide (SCSP) isolated fromStichopus japonicuwere investigated. Moreover, in order to enhance its solubility in an alcohol solution, it was depolymerized using a photocatalytic reaction, and the photocatalytic degradation products (dSCSPs) with an average molecular weight of less than 2 kDa were studied and compared with SCSP. They were characterized by a series of chemical and spectroscopy methods and the oligosaccharide fragments in the dSCSP were further identified by HPLC-MSn analysis. Then, the in vivo experiment showed that the addition of SCSP or dSCSP to Baijiu could alleviate alcoholic liver injury in mice. Further analysis also revealed their protective effect in reducing oxidative stress damage and their regulation of the metabolism of phosphatidylcholine (PC) and phosphatidylethanolamine (PE) in the liver. Of note, dSCSP was more effective at reducing the level of malondialdehyde in the liver. These findings indicate that the addition of sea cucumber polysaccharide or its low-molecular-weight derivative in Baijiu has the potential to alleviate alcoholic liver injury.
研究领域:
药理学
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肝病学
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生物化学
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食品科学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2020
·
IF 6.306
Peipei Yin,Airong Jia,Kirsten Heimann,Miansong Zhang,Xin Liu,Wei Zhang,Changheng Liu
Hot water pretreatment of sea cucumbers potentially changes nutritional benefits. This study aimed to quantify hot water pretreatment-induced changes in metabolite profiles of sea cucumber body walls. ICP-OES, GC-MS, and LC-MS analyses of untreated- (UT-BW), hot water-treated body walls (HW-BW) of Apostichopus japonicus , and the hot water extract (HW-E) determined significant losses of minerals (25–50% w/w), protein (~11% w/w), carbohydrate (33% w/w), saponins (~41% w/w), and spermidine (100%), a potential antipsychotic from hot water-treated samples. Multivariate comparisons of HW-BW with UT-BW and HW-BW with HW-E showed increases in amino acids and fatty acids, suggesting hot water-induced degradation or transformation or easier extraction of protein, lipid or other components. Presence of 80 to 88.5% of compounds in the HW-E and lower DHA, EPA and glycerophospholipids levels in HW-BW suggested extraction of these metabolites. These data indicate that novel processing technologies are required to preserve the full nutritional benefits of sea cucumbers.
研究领域:
海洋生物学
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食品科学
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营养学
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代谢组学
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）