FOOD CHEMISTRY
·
2022
·
IF 9.231
Xin-Yu Jiang,Jia-Nan Yan,Lin Li,Wen Sun,Bin Nie,Hai-Tao Wu
The involvement of hydrogen bonding and hydrophobic interactions in a mixture of scallop ( Patinopecten yessoensis ) male gonad hydrolysates (SMGHs) and guar gum (GG) or locust-bean gum (LBG) was investigated using guanidine hydrochloride (GuHCl) and urea treatments in this study. The addition of GG and LBG (5.56 mg/mL) increased the viscosity of SMGHs at 0.1 s - 1 by almost 2.5-fold and 1.7-fold, respectively, reaching 254.8 and 177.0 Pa·s. After treatment with GuHCl or urea, the mixed gels (SMGHs/GG and SMGHs/LBG) became relatively transparent and more fluid, as the viscosity significantly reduced. Moreover, changes of moisture distribution and conformational characteristics suggested that hydrogen bonding and hydrophobic interactions were the main intermolecular forces in the mixed gels of SMGHs and GG or LBG. Furthermore, the SMGHs/GG and SMGHs/LBG mixtures yielded strong gels with viscous network structures, indicating that these materials can be used as thickening agents in food systems.
研究领域:
食品科学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
BIOMEDICINE & PHARMACOTHERAPY
·
2019
·
IF 3.743
Hao Guo,Xinhuan Wan,Fengju Niu,Jujie Sun,Chenxiao Shi,Jessica Meng Ye,Changzheng Zhou
In this study, we aimed to evaluate the antiviral effect of total flavonoids extracted from Robinia pseudoacacia cv. idaho (RPTF) in vivo and its toxicity on rats with oral gavage. RPTF was prepared by percolation with 70% ethanol for 24 h and its antiviral effect on different kinds of viruses was evaluated in vitro by MTT staining. The long-term toxicity of RPTF on rats was evaluated through the detection of general behavior, body weight, food intake and related organ tissue sections of experimental animals. We found that RPTF produced significantly inhibitory effects on HSV-1 and EV-71 viruses with the therapeutic index TI values 113.8 and 46.2, respectively. Moreover, toxicity evaluation in vivo showed no significantly adverse effects in rats, indicating that RPTF was safe in use. In conclusion, we demonstrated that RPTF, natural compounds in the Chinese traditional medicine, could act as promising and effective antiviral therapeutics with relative safety in use.
研究领域:
药理学
·
病毒学
·
毒理学
✓ 已验证
DOI
PubMed
高影响因子
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
·
2018
·Vol. 392
·期 2
·177-187
·
IF 2.238
Qiao Zhe,Cheng Yao,Liu Shiyuan,Ma Zhenchuan,Li Shaomin,Zhang Wei
Casticin, a flavonoid isolated from Vitex species, has been found to have anti-tumor property in multiple human cancers. The present study aimed to investigate the effect of casticin on the proliferation and apoptosis of esophageal cancer (EC) cells, and further illustrate the underlying mechanisms. In in vitro studies, human EC cell lines TE-1 and ECA-109 were treated with various concentrations of casticin (low-, middle-, and high-dose groups). The results showed that casticin dose-dependently inhibited the proliferation and clonogenicity of EC cells and induced cell cycle arrest in sub-G1 and G2 phases. Furthermore, casticin markedly enhanced EC cell apoptosis as detected by flow cytometry and Hoechst 33342 staining. The level of anti-apoptotic Bcl-2 protein was decreased, while the levels of pro-apoptotic Bax, cleaved-caspase-3, cleaved-caspase-9, and cleaved-PARP were conversely increased in casticin-treated TE-1 and ECA-109 cells. Moreover, casticin decreased the mitochondrial membrane potential and increased the release of mitochondrial cytochrome C into cytoplasm. In addition, the JNK signaling pathway was involved in casticin-medicated anti-proliferation and pro-apoptosis. Cells pretreated with SP600125, a JNK pathway inhibitor, partially abolished the effect of casticin. Finally, the anti-tumor property of casticin was confirmed in in vivo xenograft models. Overall, we provided both in vitro and in vivo evidences that casticin inhibited the proliferation and induced apoptosis of EC cells, and the anti-tumor action of casticin was mediated, in part, by the mitochondrial-dependent apoptosis and the activation of JNK signaling pathway.
研究领域:
肿瘤学
·
药理学
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2024
·Vol. 59
·期 8
·5430-5439
·
IF 2.6
Hao Cheng,Pengcheng Zhou,Jun Mei,Jing Xie
Summary The quality of fish is easily damaged during the frozen process, and the ice glazing technology can better maintain the quality of frozen fish. However, the traditional ice glazing technology is not yet stable and some of the properties need to be improved. This research optimised ice glazing technology by adding different antioxidants to the traditional ice glazing, which improved the stability of the ice glazing and lessened the dry consumption of mackerel during frozen storage. The lipid oxidation and protein denaturation of mackerel were inhibited with the addition of antioxidants to the ice glazing, the freshness of mackerel was maintained better, and the production of histamine was also decreased during frozen storage. It was found that the ice glazing with the addition of antioxidant bamboo leaves (AOB) had better water retention, lower TVB-N value, and histamine content compared to the addition of theaflavins (TF) and sodium isovitamin (VC). Moreover, the sample treated by the ice glazing with AOB showed less lipid oxidation and protein denaturation. Therefore, ice glazing with the addition of AOB could be an optional method for relieving the quality deterioration of mackerel during frozen storage. This research also provides insights into the coupling of antioxidants and ice-coating techniques.
研究领域:
食品科学
·
水产加工与保鲜
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Ruxia Ning,Xichuang Cheng,Fuhou Lei,Jiufang Duan,Kun Wang,Jianxin Jiang
Natural and high-quality biomass-based coating films are considered promising packaging to consumers. However, the poor mechanical properties and weak antimicrobial activity of biomass materials have limited their practical application. A cleaner and low-cost strategy is used to prepare antimicrobial, self-recovery, and biocompatible coating films using tamarind kernel powder (TKP) and chitosan (CS). The TKP protein and chitosan chains were covalently cross-linked with tetrakis(hydroxymethyl)phosphonium chloride (THPC) to form a three-dimensional network based on THPC-amine dynamic bonds, and act as a sacrificial bond. Then, the hydrogen bond forms an interpenetrating network to build a strong multi-network film. Thus, the THPC multi-crosslinking TKP based films showed enhanced stretchable property (increased from 3.23 % to 77.54 %), and self-recovery after 30 min of recovery. Additionally, the film has been found to exhibit low water vapor permeability, low oxygen transmittance rate, and excellent antimicrobial efficiency (maximum inhibition zones: 24.39 mm). Moreover, the prepared films were demonstrated to be biocompatible and non-hemolytic based on cell viability and hemolytic activity assays. The method described herein could broaden the scope of biomass-based materials in the realm of antimicrobial coating films.
研究领域:
高分子材料科学
·
食品包装工程
·
生物医学材料
·
天然高分子化学
·
抗菌材料
·
可再生资源利用
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2021
·
IF 6.953
Haiyan Tian,Dong Xiang,Congfa Li
Herein, improvement of the stability of the water-in-oil-in-water (W/O/W) emulsions by addition of xanthan gum (XG)/locust bean gum (LBG) mixture in the inner water phase was aimed. The impact of XG/LBG mixture on the physical stability, microstructure and rheological properties of W/O/W emulsions was investigated. It was found that, compared with the control emulsions, the presence of XG/LBG mixture could improve the stability of W/O/W emulsions against coalescence. The tea polyphenols (TPPs) and XG/LBG mixture were simultaneously included in the internal aqueous phase of the double emulsion and stored at 25 and 40 °C in the dark for 28 d. The results showed that XG/LBG mixture not only had a protective role for TPPs encapsulated in the internal water phase, but also maintained more than 50% of the antioxidant capacity of TPPs.
研究领域:
食品科学
·
胶体与表面化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ELECTROCHIMICA ACTA
·
2024
·
IF 5.5
Hamouda Adam Hamouda,Anas Ramadan,Leila Mahmoud Mokhtar,Suad Atitalla Ageeb,Mohammed Hassan Ibrahim,Abir EM Ajab,Mohammed Bahreldin Hussein,Adam A. Farah,Elfatih A Hassan,Guofu Ma
Effective energy storage has now become increasingly critical as global energy demand has skyrocketed. Asymmetric supercapacitors using electrodes made of acacia wood-derived carbon doped with MnO 2 . A hydrothermal process was used to synthesize the AWPC/MnO 2 composite. Techniques such as XRD , SEM, TEM, XPS, FTIR spectra, Ramman spectra, and N 2 adsorption-desorption isotherms. We looked at the electrochemical properties of the materials we made in an electrolyte using cyclic voltammetry (CV), galvanostatic charge-discharge (GCD), and electrochemical impedance spectroscopy (EIS). A hydrothermal synthesis approach is used to graft MnO 2 wire-like nanostructures onto acacia wood-derived activated carbon. Good electrochemical performance is shown by the carbon/MnO 2 hybrid composite electrode, which has high specific capacitances of 301 F g − 1 at 500 mA s − 1 , low Tafel slope of 54 mV dec −1 , which is attributed to the synergistic influences between MnO 2 and waste biomass-produced carbon. Moreover, an asymmetric supercapacitor operating in the voltage range of 1.8 V and exhibiting a high energy density of 25 Wh kg −1 at a power density of 450 W kg −1 demonstrates the significant potential of the activated carbon/MnO 2 composite generated from synthesized biomass. The AWPC/MnO 2 's oxygen evolution reaction (OER) activity was checked to see how well the improved nanostructure could be used. The results offer a fresh perspective on the fabrication of electrochemical energy storage devices with outstanding performance.
研究领域:
能源材料科学
·
电化学
✓ 已验证
产品关联
DOI
高影响因子
Materials Today Communications
·
2025
·
IF 4.5
Xiaomei Liu,Na Wang,Huaiwu Peng,Zhenshi Zhang,Ziqiang Lei
Severe water scarcity and soil salinization have emerged as persistent ecological challenges threatening global agricultural sustainability. In this study, a molecularly engineered functional crosslinking agent was successfully synthesized through molecular-level surface modification of γ-alumina nanoparticles. Subsequently, a tragacanth gum-g-polyacrylic acid / acrylic acid-aluminium oxide (TG-g-PAA/AA-Al 2 O 3 ) exhibiting dual anti-leakage and salt-resistant properties was fabricated via aqueous solution polymerization using acrylic acid as the primary monomer. Systematic characterization revealed that the incorporation of 0.8 wt% bifunctional polymeric composite significantly enhanced material performance, demonstrating 18.4 % improvement in anti-leakage properties and 24.7 % improvement in salt-resistant compared to the control group. This innovative material integrates water conservation with saline soil remediation, offering dual-functional benefits for arid region management: simultaneously addressing water resource preservation while facilitating agricultural land reclamation. The proposed methodology establishes a novel theoretical framework for coordinated management of water and soil resources in drought-prone ecosystems, providing new insights for sustainable agriculture development in arid/semi-arid regions.
研究领域:
农业生态学
·
环境功能材料
·
土壤修复
·
水土资源管理
·
高分子化学
·
干旱区生态工程
✓ 已验证
产品关联
DOI
高影响因子
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
·
2021
·Vol. 57
·期 3
·372-379
·
IF 2.252
Li Xiuyu,Zhang Zijie,Ren Yunhui,Feng Yue,Guo Qi,Dong Li,Sun Yuhan,Li Yun
Tetraploid black locust ( Robinia pseudoacacia L.) has advantages over diploid varieties in growth and stress resistance. Previous studies had shown that tetraploid black locust can be induced only by treating shoot tips of the germinating seeds with colchicine. In order to establish a simpler and more efficient tetraploid induction method in black locust, we induced tetraploid plants by treating the hypocotyls from in vitro –grown seedlings with different concentrations of colchicine, and then provided a method for tetraploid identification based on stomatal size and density in this study. The highest induction efficiency was 53.33% by treating hypocotyl explants with 70 mg·L −1 colchicine for 2 d. Tetraploid black locust was significantly different from diploid in morphological characteristics. Leaf area, leaf thickness, chlorophyll content, stomatal length, and stomatal width were greater in tetraploid plants than in diploid plants, whereas the opposite was true for leaf aspect ratio, stomatal index, and stomatal density. The differences in stomatal size and density between diploid and tetraploid were nearly two times; therefore, size and density of stomata can be used as a stable and distinct marker to rapidly screen tetraploids. The results of this study suggested that the hypocotyl was an ideal material to induce tetraploid black locust in vitro and confirmed that stomatal size and density could be an effective and reliable marker to identify ploidy level in black locust.
研究领域:
植物科学
·
生物技术
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2022
·
IF 6.449
Shuai Wang,Yunxiao Wang,Shunxin Wang,Shuang Guo,Dongyu Gu,Jifeng Wang,Yi Yang
In this study, R. pseudoacacia flowers exhibited potential lipase ( Aspergillus niger ) inhibitory activity. To quickly explore the active compound, ligand fishing was adopted for screening the ligand from R. pseudoacacia flowers by lipase immobilized on magnetic polyamidoamine dendrimer-coated multi-walled carbon nanotubes . After ligand fishing, the target compound was separated purposefully by optimizing the separation conditions of high-speed counter-current chromatography (HSCCC). As results, kaempferol with the IC 50 of 327.64 ± 15.33 μg/mL was separated and identified. The further molecular docking results indicated that kaempferol was interacted with amino acid residue in the active site of lipase through van der Waals force, hydrogen bonds and Pi-Pi T-shaped interactions, yielding the binding energy of − 6.43 kcal/mol. The present method not only revealed the lipase inhibitory substances in R. pseudoacacia , but also provided an effective means for the screening of other natural substances.
研究领域:
生物化学
·
纳米技术
·
药理学
·
植物科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF FOOD ENGINEERING
·
2021
·
IF 5.354
Anlei Ge,Shahid Iqbal,Timothy V. Kirk,Xiao Dong Chen
In this study we determined the effect of whey protein isolate-polysaccharide combinations in aqueous phases, on modulating the rheology and microstructural properties of 20 wt % oil-in-water emulsions. The aqueous phases of three emulsions consisted of combinations of 4 wt % whey protein isolate (WPI) and 0.5 wt % Locust bean gum, iota-carrageenan (IC), or chitosan separately (pH 4, 100 mM NaCl), while the oil phase was soybean oil . It was observed that all heated emulsions had higher moduli and viscosities than unheated systems. This behavior could be due to greater protein-polysaccharide interactions after thermal treatment, leading to highly viscous and elastic materials. Observed microstructures also supported the rheological results, and showed that thermal treatment led to denser gel-networks formation in heated emulsions, particularly in WPI-IC system. This study may provide significant impact in the production of semi-solid O/W emulsified foods with improved texture and rheology, but reduced total fat content.
研究领域:
食品科学
·
化学工程
·
材料科学
✓ 已验证
DOI
高影响因子
ENZYME AND MICROBIAL TECHNOLOGY
·
2015
·
IF 2.322
Haoyu Zang,Shanshan Xie,Huijun Wu,Weiduo Wang,Xiankun Shao,Liming Wu,Faheem Uddin Rajer,Xuewen Gao
A novel thermostable mannanase from a newly isolated Bacillus pumilus GBSW19 has been identified, expressed, purified and characterized. The enzyme shows a structure comprising a 28 amino acid signal peptide, a glycoside hydrolase family 5 (GH5) catalytic domain and no carbohydrate-binding module. The recombinant mannanase has molecular weight of 45 kDa with an optimal pH around 6.5 and is stable in the range from pH 5–11. Meanwhile, the optimal temperature is around 65 °C, and it retains 50% relative activity at 60 °C for 12 h. In addition, the purified enzyme can be activated by several ions and organic solvents and is resistant to detergents. Bpman5 can efficiently convert locus bean gum to mainly M2, M3 and M5, and hydrolyze manno-oligosaccharides with a minimum DP of 3. Further exploration of the optimum condition using HPLC to prepare oligosaccharides from locust bean gum was obtained as 10 mg/ml locust bean gum incubated with 10 U/mg enzyme at 50 °C for 24 h. By using this enzyme, locust bean gum can be utilized to generate high value-added oligosaccharides with a DP of 2–6.
研究领域:
酶学
·
生物技术
·
食品科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）