Journal of Oleo Science
·
2013
·
IF 1.242
Xin Liu,Teng Chen,Xuesong Liu,Yong Chen,Longhu Wang
The present study investigated the feasibility of ostrich oil utilizing as a promising vehicle for improved skin permeation of sinomenine with reference to vaseline matrix containing different content of chemical enhancers. The fatty acid composition of ostrich oil was analyzed by GC-MS. Penetration enhancing potential of ostrich oil on permeation of sinomenine across rat abdominal skin in vitro was studied using an automatic diffusion cell apparatus. The content of sinomenine percutaneous absorbed was determined by HPLC. Various parameters viz. steady-state skin flux (Jss), permeability coefficient (kP), cumulative amount of sinomenine (Q) and enhancement ratios (ER) were calculated from the permeation data. Fick’s law of diffusion and Scheuplein kinetic were used to evaluate the transdermal absorbent enhancement of ostrich oil to sinomenine. Ostrich oil showed significant penetration effect on sinomenine compared with vaseline matrix containing different content of chemical enhancers, the density sequence as follow: 2% Azone > ostrich oil > 1% Azone plus 1% propylene glycol > 1% Azone > 3% Azone > 1% propylene glycol. The percutaneous endosmic rate constant (Jss) and permeability coefficient (kP) of sinomenine in ostrich oil through rat skin were 10.01 μg/cm2/h and 0.087, respectively. Ostrich oil produced stronger enhancement (ER = 24.31) with greater cumulative amount of drug permeated (255.53 μg/cm2) up to 24 h and caused no skin irritation. The drug release of sinomenine was coincided with Fick’s equation. In summary, ostrich oil containing fatty acids is proposed as a promising adjuvant for use in cosmetics and pharmaceuticals for improved permeation of drug.
研究领域:
药剂学
·
皮肤科学
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DOI
高影响因子
Journal of Cosmetic Dermatology
·
2019
·Vol. 18
·期 5
·1564-1574
·
IF 1.311
Ying Tang,Wendan He,Yanping Wu,Rui Cai
Summary Background The interest toward dyeing hairs with plant colorants has grown in popularity considering its low-toxic nature. However, researches reporting plant hair dyes are limited and the potential adverse effects of irritation are unclear. Objectives This study is aimed to provide an avenue by which to more accurately assess the dyeing efficiency and irritation potentials of plant hair dyes. Methods Four extracted plant colorants were incorporated in hydrogel hair dyes that were directly applied on unbleached gray human hairs. Their dyeing performances and the effect of an iron (Ⅱ) mordant were photometrically measured in CIELab coordinates and color strength. The eye and skin irritancy was assessed by combining various in vitro methods, including bovine corneal opacity and permeability (BCOP) assay in combination with histopathological analysis, Hen’s egg test on chick chorioallantoic membrane (HET-CAM) and a test on reconstructed human epidermis models. Results The investigated hair dyes exhibited desirable dyeing efficiency on human hairs. Post-treatment with the iron (Ⅱ) mordant caused a significant increase in color strength with subtle changes to the hue of dyed color. In the irritation testing, the four hair dyes were categorized as slight-to-mild eye irritants but possessed no skin irritation potential, while the mordant was determined as a non-irritant. Conclusions The results demonstrate the efficacy of a multi-analytical approach for in vitro assessment of various plant colorants for hair dyeing. The investigated plant extracts are suitable for producing viable colors on human hairs and may serve as a low-irritating alternative to the synthetic hair dyes.
研究领域:
生物学
·
医学
·
化妆品科学
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DOI
高影响因子
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
·
2025
·
IF 4.9
Jiayu Li,Huanfan He,Jun Zhu,Shujing Li,Yifan He,Ze Zhang,Xiaojing Pei
Nanoemulsions (NE) are attractive for cosmetic applications due to ensure improved steadiness and efficient penetration of bioactive components, leading to enhanced efficacy. Hydroxypinacolone Retinoate (HPR) is an ingredient with great potential in combating the effects of aging, but its formulation is often unstable. In order to improve the solubility, bioavailability and stability of HPR, two kinds of oil-in-water (O/W) nanoemulsions were developed by high-pressure homogenization technology. These two nanoemulsions used GTCC and dimethyl isosorbide as the oil phase, respectively, which leads to different properties. Average particle size was (101 ± 0.02 nm) (72 ± 0.03 nm), Zeta potential was (-16.23 ± 0.15) (-18.21 ± 0.02). Particle size, polydispersity index, and potential were recorded for seven different conditions, which showed good physical and chemical stability over 90 days. Compared to free HPR, the retention of NE at 60 days under light and 45 °C was improved by 10% (G-NE) and 8% (D-NE), respectively. Encapsulation resulted in improved stability and bioavailability of HPR. In the in vitro diffusion cell assay, the skin retention of the two nanoemulsions was 17.02 μg/mL and 29.17 μg/mL, which was much higher than that of the free HPR, which was 4.08 μg/mL (P<0.01). Both the erythrocyte hemolysis test and the human patch test showed that the nanoemulsion reduced the stimulation of HPR. This study provides a practical method to increase the utilization of HPR in cosmetic formulations, thereby improving efficacy.
研究领域:
化妆品科学
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药剂学
·
生物医学工程
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产品关联
DOI
高影响因子
JOURNAL OF INCLUSION PHENOMENA AND MACROCYCLIC CHEMISTRY
·
2019
·Vol. 94
·期 1
·85-94
·
IF 1.429
Li Shuhan,Tang Yue,Zhang Xinru,Dou Yushun,Shen Xiangchun
This research is to study on preparation and characterization of diclofenac sodium β-cyclodextrin (DS-β-CD) inclusion complex eye drops in order to improve the solubility of diclofenac sodium (DS) and reduce the irritation of DS eye drops in the market. First, DS-β-CD inclusion complex was prepared by co-precipitation method and then mixed with excipients to prepare inclusion complex eye drops. Finally, instruments were used to determine the basic properties of eye drops and animal experiments were used to investigate the irritation of the DS-β-CD inclusion complex eye drops. The drug content, the encapsulation efficiency and the yield of DS-β-CD inclusion complex were respectively 17.11, 84.20 and 60.00%. And the DS-β-CD inclusion complex was identified by FTIR, DSC, XRD and 1 HNMR. The solubility of the inclusion complex was 20 times more than DS. The pH value, the osmotic pressure, the viscosity and the drug content of the eye drops were respectively 7.20, 298 mosmol/kg, 4.88 mPa s and 97.98%. In brief, DS-β-CD inclusion complex eye drops had stable properties, high solubility and the test items met the requirements. The eye drops were almost non-irritating, significantly reducing the irritation of the original prescription (P < 0.01).
研究领域:
药剂学
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药物化学
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Bioengineering and Biotechnology
·
2024
·
IF 4.3
He Wei,Zhang Xinya,Zou Yangxiaoyu,Li Ji,Chang Le,He Yu-Cai,Jin Qiuheng,Ye Jianren
Introduction: Circular RNAs (circRNAs) are endogenous noncoding RNAs (ncRNAs) with transcriptional lengths ranging from hundreds to thousands. circRNAs have attracted attention owing to their stable structure and ability to treat complicated diseases. Our objective was to create a one-step reaction for circRNA synthesis using wild-type T7 RNA polymerase as the catalyst. However, T7 RNA polymerase is thermally unstable, and we streamlined circRNA synthesis via consensus and folding free energy calculations for hotspot selection. Because of the thermal instability, the permuted intron and exon (PIE) method for circRNA synthesis is conducted via tandem catalysis with a transcription reaction at a low temperature and linear RNA precursor cyclization at a high temperature.Methods: To streamline the process, a multisite mutant T7 RNA polymerase (S430P, N433T, S633P, F849I, F880Y, and G788A) with significantly improved thermostability was constructed, and G788A was used.Results: The resulting mutant exhibited stable activity at 45°C for over an hour, enabling the implementation of a one-pot transcription and cyclization reaction. The simplified circRNA production process demonstrated an efficiency comparable to that of the conventional two-step reaction, with a cyclization rate exceeding 95% and reduced production of immunostimulatory dsRNA byproducts.
研究领域:
分子生物学
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生物化学
·
合成生物学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2024
·Vol. 416
·期 12
·2995-3006
·
IF 3.8
Guan Tian,Chen Sha,Chen Yanhong,Wang Yu,Yu Xiaoqin,Huang Xinan,Liu Qing,Lei Hongtao,Yang Qunhua
Stimulant laxatives were recently found to be abused in slimming foods, resulting in harmful effects on consumers. To ensure the safety of relative products, sensitive yet multiplex immunoassays are crucial in rapid screening of stimulant laxatives. However, there are few immunoassays for these substances, and even less for broad-specific recognition. Thus, in this work, four theoretically promising haptens of emerging stimulant laxative bisacodyl were rationally designed using molecular modeling and synthesized to immune animals, whose feasibility was confirmed by the obtained broad-specific antibody. Based on this unique antibody, a highly sensitive multiplex competitive indirect enzyme-linked immunosorbent assay (ciELISA) was established with low limits of detection for bisacodyl, sodium picosulfate, and BHPM (0.23, 13.68, and 0.11 ng/mL). In spiked sample recovery test and real sample detection, this ciELISA exhibited acceptable consistency with the validation method, demonstrating high accuracy and applicability of our method. This reliable multiplex ciELISA proceeds the rapid screening of stimulant laxatives in slimming foods. Graphical abstract
研究领域:
食品安全
·
免疫学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2013
·Vol. 14
·期 12
·24305-24319
·
IF 2.464
Jian Chen,Gui-Dong Huang,Si-Rong Tan,Jiao Guo,Zheng-Quan Su
This study aimed to research the preparation and content determination of capsaicin-chitosan microspheres (CCMS) enteric coated tablets. The core tablets were prepared with the method of wet granulation. Nine formulae were designed to determine the optimal formula of the core tablet. Eudragit L100 was used to prepare the CCMS enteric-coated tablets. The effect of enteric coated formulation variables such as content of talc (10%, 25% and 40%), plasticisers (TEC and DBS), dosage of plasticiser (10%, 20% and 30%) and coating weight (2%, 3% and 5%) were evaluated for drug release characteristics. Thein vitrorelease was studied using 0.1 N HCl and pH 6.8 phosphate buffer. Enteric coated tablets without ruptures or swelling behaviour over 2 h in 0.1 N HCl indicated that these tablets showed acid resistance. The accumulated release rate in phosphate buffer (pH 6.8) revealed that the prepared tablets were able to sustain drug release into the intestine and a first-order release was obtained for capsaicin. This research is the first report of the preparation and content determination of CCMS enteric coated tablets. The sustained release behavior of enteric coated formulations in pH 6.8 phosphate buffer demonstrated that it would be a potential drug delivery platform for sustained delivery of gastric irritant drugs.
研究领域:
药剂学
·
药物递送系统
✓ 已验证
DOI
高影响因子
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
·
2017
·
IF 3.756
Xin Liang,Yu-lin Chen,Xiao-jing Jiang,Sheng-mei Wang,Ji-wen Zhang,Shuang-ying Gui
The main objective of this study was to develop reversed hexagonal (H II ) mesophase for transdermal delivery of methyl salicylate . The formulation was prepared, characterized and evaluated for its skin penetration in vitro and skin retention in vivo. Preliminary pharmacodynamics and skin irritation were also investigated. The formulation was identified as hexagonal structure. In vitro study exhibited that H II mesophase enhanced the skin permeation by delivering 2.61 times more methyl salicylate than the commercially available cream. Meanwhile, H II mesophase presented higher bioavailability as AUC (0–24) and AUC (0–∞) were 32.894 μg·mL − 1 and 32.935 μg·mL − 1 respectively, while the cream were 12.791 μg·mL − 1 and 12.970 μg·mL − 1 . Preliminary pharmacodynamics studies demonstrated that H II mesophase possessed anti-inflammatory and analgesic effects for inhibiting paw edema , granuloma and pain. MeSa H II mesophase showed no skin irritation on the normal rat skin. Thus, H II mesophase was considered as an effective delivery system for MeSa.
研究领域:
药剂学
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药物递送系统
·
皮肤科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CELLULOSE
·
2018
·Vol. 25
·期 9
·5415-5426
·
IF 3.809
Gu Jingwei,Yuan Lingjun,Zhang Ze,Yang Xinhui,Luo Junxuan,Gui Zifan,Chen Shiguo
Abstract We developed an isocyanate group containing quaternary ammonium salt (IQAS) as a potential antimicrobial finishing agent that can be stored in a high purity form for more than a year in dry environments to permit convenient transportation and storage. Additionally, we report a facile and eco-friendly finishing technique to fabricate durable antimicrobial cotton fabrics using IQAS as an antimicrobial finishing agent by a dipping–padding–drying process. IQAS was bound onto the surface of cotton fabrics by a covalent bond to obtain a cotton fabric with excellent bactericidal activity, tearing strength, breaking elongation, bending rigidity, water vapor permeability, surface roughness and smoothness. The antimicrobial rates of these fabrics reached 92.2% and 98.5% against gram-negative bacterium Escherichia coli and gram-positive bacterium Staphylococcus aureus , respectively, even after 50 laundering cycles. These values are much higher than the reference antimicrobial rates of AAA class antimicrobial fabrics, as well as those of conventionally finished cotton fabrics, indicating that the IQAS finished cotton fabrics maintained excellent antimicrobial activity even after long-term repeated launderings. Moreover, the results indicate that the IQAS-treated cotton fabrics could improve the breaking strength (increased by 13.5% in the warp direction and 20.3% in the weft direction), the bursting strength (increased by 11.9%), the air permeability (increased by 12.6%) and the hydrophilicity compared to untreated cotton fabrics. Additionally, our non-leaching antimicrobial cotton fabrics treated with IQAS were non-toxic and showed no skin stimulation. Therefore, IQAS and the antimicrobial finishing technique reported here have great potential applications in antimicrobial fabrics used in hospitals, hotels and other susceptible situations. Graphical abstract An isocyanate group containing quaternary ammonium salt that permits convenient transportation and storage was developed as a potential antimicrobial finishing agent to fabricate excellent antimicrobial cotton fabrics with well-preserved physical properties and security using a dipping–padding–drying process.
研究领域:
纺织科学
·
材料科学
·
生物医学
✓ 已验证
产品关联
DOI
高影响因子
BIOORGANIC CHEMISTRY
·
2023
·
IF 5.1
Xuejiao Wang,Yuting Liu,Tongyue Wu,Biaofeng Gu,Hao Sun,Huanling He,Haiqin Gong,Hu Zhu
The commercial cationic surfactants (CSAa) with quaternary ammonium (QA) groups have proved to be broad-spectrum bactericide against bacteria, fungi, and viruses. Nevertheless, they inevitably exhibit potent irritation on the skin. In this work, we systematically investigated the regulatory mechanism of the host-guest supramolecular conformation with β-cyclodextrin (β-CD) on the bactericidal performance and skin irritation of CSAa with different head groups and chain lengths. When the ratio of incorporated β-CD is not greater than 1:1, the bactericidal efficiency of CSAa@β-CD (n > 12) remained above 90 % due to the free QA groups and hydrophobic fraction that can act on negatively charged bacterial membranes. And once the ratio of β-CD exceeded 1:1, the β-CD attracted to the bacterial surface by hydrogen bonding might prevent CSAa@β-CD from acting on bacteria, resulting in a decrement in antibacterial performance. Even so, the antibacterial activity of CSAa with long alkyl chains (n = 16, 18) was independent from the complexation of β-CD. Accordingly, both the zein solubilization assay and the neutrophil migration assay on zebrafish skin evidenced that β-CD attenuated the interaction of surfactant with skin model proteins and the inflammatory effect on zebrafish, thereby enhancing skin mildness. In this way, we hope to create a simple but effective brainpower using the host-guest approach to guarantee both bactericidal efficiency and skin mildness without modifying the chemical structure of these commercial biocides.
研究领域:
生物学
·
医学
·
化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PHARMACEUTICAL RESEARCH
·
2014
·Vol. 31
·期 8
·1907-1918
·
IF 3.952
Chen Yang,Cun Dongmei,Quan Peng,Liu Xiaochang,Guo Wenjia,Peng Lihua,Fang Liang
Purpose Saturated long-chain esters of isopulegol were synthesized and their activities as permeation enhancers for transdermal delivery of amlodipine and flurbiprofen were investigated, in contrast to the saturated fatty acids and isopulegol, as well as their physical mixtures. Methods
In vitro permeation experiments, confocal laser scanning microscopy (CLSM) and attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy were introduced to investigate the regulation of enhancers in the skin permeability and biophysical properties. With in vitro cytotoxicity test and in vivo erythema model, the skin irritation of enhancers was evaluated. Results The esters significantly increased the permeation of amlodipine and flurbiprofen, whereas saturated fatty acids and isopulegol had no such effect and even decreased the drug permeation when they were used alone or in combination. These results were supported by CLSM and ATR-FTIR studies, which revealed that only the esters could decrease the order of the alkyl chains in the skin lipids. Additionally, almost no skin irritation and cytotoxicity were observed for these esters. Conclusions Saturated long-chain esters of isopulegol are shown to be suitable permeation enhancers for transdermal drug delivery. Covalent attachment of isopulegol and saturated fatty acids might represent a promising strategy to design novel and potent permeation enhancers.
研究领域:
药剂学
·
生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
·
2014
·
IF 1.357
Yongli Shi,Yue Li,Jianming Wu,Weiguo Wang,Anjie Dong,Jianhua Zhang
Transdermal drug delivery systems (TDDS) had achieved significant success in medical practice, but still suffered from adhesion failure and skin reaction due to the occlusive properties of hydrophobic pressure sensitive adhesives (PSAs). In order to solve these problems, a novel TDDS patch based on self-adhesive Janus nanofibrous film was prepared by a multilayered electrospinning. This multifunctional patch was a bilayer structure. The subjacent layer was a hydrophobic and adhesive fibrous layer electrospun from polyacrylate PSA (HPSA), and the upper backing layer was a hydrophilic cross-linked poly (vinyl alcohol) (c-PVA) nanofibrous film. The structures of the HPSA/c-PVA composite fibrous films were characterized and their application properties, including adherence performance, water vapor permeability, water-penetration, release characteristics, and skin irritation were evaluated. The results indicated that the HPSA/c-PVA composite fibrous films could provide suitable adhesive properties for TDDS application, excellent capacity for drug loading and release, aesthetical appearance and high safety for use on the skin. Especially, due to the nanofibrous network structures and the hydrophobic–hydrophilic wettability gradient from hydrophobic HPSA layer to the hydrophilic c-PVA layer, the Janus films possessed high breathability and monodirectional water-penetration. Water could penetrate from the hydrophobic to the hydrophilic side, but could not permeate through in the opposite direction. This may provide a feasible solution to the problems caused by the water, sweat, or wound exudate on the skin, when the hydrophobic PSAs were used as matrix for TDDS and wound dressing patches.
研究领域:
材料科学
·
药剂学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）