Heliyon
·
2022
·
IF 3.776
Wenjun Zhu,Qianqian Gu,Boyan Liu,Yanhong Si,Huirong Sun,Jingjie Zhong,Yi Lu,Dan Wang,Junli Xue,Shucun Qin
As an antioxidant, anti-inflammatory and anti-apoptotic agent, hydrogen (H 2 ) shows a promising potential in basic and clinical research against various diseases owing to its safety and efficacy. However, knowledge involving its underlying mechanisms of action, dosage effects, and dose duration remains limited. Previously, the dynamics of H 2 concentrations in different tissues of rats after exogenous H 2 inhalation had been detected by our team. Here, sequential changes of H 2 concentrations in different tissues of another most commonly used experimental rodent mice were monitored in real time with an electrochemical H 2 gas sensor during continuous different concentrations of H 2 inhalation targeting on five tissues including brain, liver, spleen, kidney, and gastrocnemius. The results showed that the H 2 saturation concentrations varied among tissues significantly regardless of the concentration of H 2 inhaled, and they were detected the highest in the kidney but the lowest in the gastrocnemius. Meantime, it required a significant longer time to saturate in the thigh muscle. By comparing the H 2 saturation concentrations of mice and rats, we found that there were no differences detected in most tissues except the kidney and spleen. Both gas diffusion and bloodstream transport could help the H 2 reach to most organs. The results provide data reference for dosage selection, dose duration determination to ensure optimal therapeutic effects of H 2 for mice experiments.
研究领域:
医学
·
生物化学
✓ 已验证
DOI
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2021
·Vol. 29
·期 7
·10858-10864
·
IF 4.223
Li Min,Wang Yayao,Ma Lu,Yan Xingfu,Lei Qian
Many quinoline (QL) derivatives are present in the environment and pose potential threats to human health and ecological safety. The acute toxicity of 30 haloquinolines (HQs) was examined using the photobacterium Vibrio fischeri. IC 50 values (inhibitory concentration for 50% luminescence elimination) were in the range 5.52 to >200 mg·L −1 . The derivative 5-BrQL exhibited the highest toxicity, with 3-ClQL, 3-BrQL, 4-BrQL, 5-BrQL, 6-BrQL, and 6-IQL all having IC 50 values below 10 mg·L −1 . Comparative molecular field analysis modeling based on the steric and electrostatic field properties of the HQs was used to quantify the impact of halogen substituents on their toxicity. QL derivative rings with larger substituents at the 2/8-positions and less negative charge at the 4/5/6/8-positions were positively correlated with acute toxicity towards V. fischeri .
研究领域:
环境科学
·
毒理学
✓ 已验证
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2020
·
IF 6.551
Yang Liu,Yang Li,Bo Pan,Xinyue Zhang,Huang Zhang,Christian E.W. Steinberg,Hao Qiu,Martina G. Vijver,Willie J.G.M. Peijnenburg
With the expanding nanotechnology, nanoparticles (NPs) embedded products are used in the agricultural sector to improve soil fertility. Thus, two typical metal oxides NPs and their mixtures were applied in different doses to evaluate the impacts on soil microbes. CuO and ZnO NPs boosted soil microbial communities as reflected by the increased number of extractable bacterial or fungal groups and the enlarged values of Chao 1, ACE, and Shannon indices. Relative abundance of some susceptible taxa such as Sphingomonadales increased with increasing concentrations of ZnO NPs, while IMCC26256 decreased with increasing concentrations of CuO NPs. The mixture of CuO and ZnO NPs did not show more promotional effects on the soil bacterial community than the sum of individual effects. Increased soil organic carbon mitigated the impacts on soil bacteria for CuO NPs, but not for ZnO NPs. As micro-nutrients, the ions released from CuO and ZnO NPs had the potential to promote soil microbial community richness and diversity. However, the positive impacts of MNPs were impaired at dosage higher than 250 mg kg −1 soil (213.08 mg kg −1 soil of Cu, 162.73 mg kg −1 soil of Zn). Thus, the application dose and soil type other than the coexistence of MNPs should be considered before the wide use in increasing agricultural productivity.
研究领域:
环境科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Foods
·
2025
·Vol. 14
·期 18
·3255
·
IF 5.1
Huowang Zheng,Fangshu Shi,Jinjun Li,Xiangyu Bian,Shuisheng Wu,Xiaoqiong Li
This study systematically investigated the dose–response relationship of resistant starch type 2 (RS2; Hi-maize 260; 0–15 g/L) on gut microbial composition, short-chain fatty acid (SCFA)/gas output, and tryptophan catabolism using an in vitro fermentation model. The highest RS2 concentration (15 g/L) elicited optimal metabolic outcomes, including maximal SCFA production; significant H2S reduction; and redirected tryptophan metabolism from potentially detrimental indoles toward neuroprotective metabolites. Microbial profiling revealed dose-dependent enrichment of saccharolytic taxa (Bifidobacterium,Lactobacillus) with concomitant suppression of proteolytic pathobionts (e.g.,Escherichia-Shigella). Correlation analyses revealed strong positive associations between beneficial microbes and both SCFAs and neuroprotective metabolites, whereas pathogenic taxa correlated inversely with these compounds. Collectively, these findings establish that functionally relevant microbiome modulation requires a sufficiently high, dose-tailored intake of RS2, providing a rational basis for precision dietary strategies aimed at improving host metabolic and gut health.
研究领域:
微生物学
·
营养学
·
代谢组学
·
胃肠病学
·
分子生物学
·
转化医学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2016
·
IF 3.169
Jian-Ping Li,Jian-Ming Guo,Er-Xin Shang,Zhen-Hua Zhu,Yang Liu,Bu-Chang Zhao,Jing Zhao,Zhi-Shu Tang,Jin-Ao Duan
Acetylsalicylic acid (Aspirin, ASA) is a famous drug for cardiovascular diseases in recent years. Effects of ASA dosage on the metabolic profile have not been fully understood. The purpose of our study is to establish a rapid and reliable method to quantify ASA metabolites in biological matrices, especially for glucuronide metabolites whose standards are not commercially available. Then we applied this method to evaluate the effects of ASA dosage on the metabolic and excretion profile of ASA metabolites in rat urine. Salicylic acid (SA), gentisic acid (GA) and salicyluric acid (SUA) were determined directly by UHPLC–MS/MS, while salicyl phenolic glucuronide (SAPG) and salicyluric acid phenolic glucuronide (SUAPG) were quantified indirectly by measuring the released SA and SUA from SAPG and SUAPG after β-glucuronidase digestion. SUA and SUAPG were the major metabolites of ASA in rat urine 24 h after ASA administration, which accounted for 50% (SUA) and 26% (SUAPG). When ASA dosage was increased, the contributions dropped to 32% and 18%, respectively. The excretion of other three metabolites (GA, SA and SAPG) however showed remarkable increases by 16%, 6% and 4%, respectively. In addition, SUA and SUAPG were mainly excreted in the time period of 12–24 h, while GA was excreted in the earlier time periods (0–4 h and 4–8 h). SA was mainly excreted in the time period of 0–4 h and 12–24 h. And the excretion of SAPG was equally distributed in the four time periods. We went further to show that the excretion of five metabolites in rat urine was delayed when ASA dosage was increased. In conclusion, we have developed a rapid and sensitive method to determine the five ASA metabolites (SA, GA, SUA, SAPG and SUAPG) in rat urine. We showed that ASA dosage could significantly influence the metabolic and excretion profile of ASA metabolites in rat urine.
研究领域:
药理学
·
分析化学
·
代谢组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2025
·
IF 12.4
Caiyun Liu,Lulu Wu,Zengshuai Zhang,Ziying Li,Mario Prejanò,Tiziana Marino,Yongsheng Tao,Yunkui Li
This study was aimed to explore the effect of α-cyclodextrin (α-CD) on color quality and stability of red wines, and to dissect the mechanism of copigmentation at molecular level. Firstly, α-CD was added to real wine before fermentation and aging dynamics were monitored. This study found that α-CD enhanced visual quality by strengthening anthocyanins maceration during fermentation, yet α-CD improved color stability by promoting formation of color-stable polymeric anthocyanins during aging. Secondly, the thermodynamics and dose-effect relationships in model wines revealed that binding between α-CD and malvidin-3- O -glucoside (Mvd-G) was a spontaneous exothermic reaction driven by enthalpy, with an optimal molar ratio of 1:5. Finally, structural characterization of host-guest complex and electronic effects of intermolecular interactions revealed that Mvd-G successfully formed host-guest complex with α-CD through hydrogen bonding and dispersion. The hydroxyl group and glucoside in the anthocyanin C ring, as well as the primary hydroxyl group of the α-CD were the key structures for forming hydrogen bonds. The results of this study provided an experimental and theoretical basis for the applications of α-CD as copigment to enhance the color quality and stability.
研究领域:
食品科学
·
化学
·
葡萄酒技术
·
分子生物学
·
量子力学
✓ 已验证
产品关联
DOI
高影响因子
CHEMICO-BIOLOGICAL INTERACTIONS
·
2023
·
IF 5.1
Xiaoting Luo,Feifei Lu,Zhiyue Yin,Zhiyun Zhou,Zhongmin Wang,Hongjian Zhang
Carboxylesterase 1 (CES1) is a hydrolytic enzyme that plays an important role in the activation or deactivation of many therapeutic agents, thus affecting their pharmacokinetic and pharmacodynamic outcomes. Using rat liver S9 as an enzyme source and enalapril as a CES1 substrate, the present study examined effects of a number of flavonoids on the formation of enalaprilat (the active form of enalapril) produced by CES1-mediated hydrolysis. While a majority of flavonoids tested showed inhibition on CES1, an unexpected hormetic effect was observed for epigallocatechin (EGC) and epigallocatechin gallate (EGCG), i.e., stimulatory effect at low concentrations and enzyme inhibition at high concentrations. Further experiments revealed that oxidative stress caused by hydrogen peroxide, arachidonic acid plus iron, and oxidized low density lipoproteins (oxLOL) reduced CES1 activity in rat liver S9 and the loss of CES1 enzyme activity could be rescued largely by EGC or EGCG. In contrast, such effects were minimal in human liver S9, probably due to the presence of a higher ratio of reduced vs oxidized forms of glutathione . The above findings suggest that the polyphenolic nature of EGC or EGCG might be responsible for rescuing CES1 activity under oxidative stress. Because of the importance of CES1 in drug activation or deactivation and rat liver S9 as a versatile in vitro system used for drug metabolism studies and drug safety assessment , caution should be exercised to avoid potential biases for data interpretation and decision making when CES1 activity in rat liver S9 is evaluated with dependency on experimental conditions.
研究领域:
药理学
·
毒理学
·
生物化学
·
药物代谢
·
酶学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2024
·
IF 12.2
Falin He,Huijian Shi,Shaoyang Hu,Rutao Liu
Currently, the binding of iron-binding protein transferrin (TF) with NPs and their interaction mechanisms have not been completely elucidated yet. Here, we probed the conformation-dependent release of Fe ions from TF induced by nano-sized polystyrene plastics (PS-NPs) using dialysis, ICP-MS, multi-spectroscopic techniques, and computational simulation. The results showed that the release of free Fe ions from TF was activated after PS-NPs binding, which displayed a clear dose-effect correlation. PS-NPs binding can induce the unfolding and loosening of polypeptide chain and backbone of TF. Alongside this we found that the TF secondary structure was destroyed, thereby causing TF protein misfolding and denaturation. In parallel, PS-NPs interacted with the chromophores , resulting in the occurrence of fluorescence sensitization effects and the disruption of the surrounding micro-environment of aromatic amino acids . Also, the binding of PS-NPs induced the formation of new aggregates in the PS-NPs-TF system. Further simulations indicated that PS-NPs exhibited a preference for binding to the hinge region that connects the C-lobe and N-lobe, which is responsible for the Fe ions release and structural alterations of TF. This finding provides a new understanding about the regulation of the release of Fe ions of iron-loaded TF through NPs-induced conformational and structural changes.
研究领域:
生物化学
·
分子生物学
·
纳米技术
·
蛋白质化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TOXICOLOGY AND APPLIED PHARMACOLOGY
·
2018
·
IF 3.616
Qiaozhu Tan,Ming Zhang,Lujing Geng,Zhenghua Xia,Cen Li,Muhammad Usman,Yuzhi Du,Lixin Wei,Hongtao Bi
Methylmercury (MeHg + ) is an extremely toxic organomercury cation that can induce severe neurological damage. Once it enters the body, methylmercury binds to amino acids or proteins containing free sulfhydryl groups. In particular, methylmercury is known to bind with human serum albumin (HSA) in human plasma; however, the effects of methylmercury-HSA conjugate (MeHg-HSA) on the central nervous system (CNS) are not fully understood. In the present study, we used the microglial cell line N9 as the target cells to evaluate the effect of MeHg-HSA on physiological function of the CNS preliminarily. The various factors in the cell culture were monitored by MTT assay, total lactate dehydrogenase assay, ELISA , qPCR, Western blot and flow cytometry techniques. The results showed that low-dose treatment with MeHg-HSA activated N9 cells, promoting cell proliferation and total cell number, enhancing NO and intracellular Ca 2+ levels, and suppressing the release of TNFα and IL1β without cytotoxic effects; while high-dose MeHg-HSA exhibited cytotoxic effects on N9 cells, including promoting cell death and increasing the secretion of TNFα and IL1β. These results indicate that MeHg-HSA causes hormesis in microglia N9 cells. Furthermore, ERK/MAPKs and STAT3 signaling pathways related to the hormesis of MeHg-HSA on N9 cells. In addition, low dose of MeHg-HSA might be viewed as something very close to a lowest observed adverse effect level (LOAEL) for N9 cells. These findings will be useful for investigating the hormesis mechanism of MeHg + and exploring the specific functions of MeHg-sulfhydryl conjugates on the central nervous system.
研究领域:
神经毒理学
·
细胞生物学
·
药理学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Metabolites
·
2024
·Vol. 14
·期 8
·449
·
IF 3.4
Min Li,Zhenfang Shang,Yonglan Ma,Huijun Zhao,Zhijing Ni,Zhaojun Wei,Xiu Zhang
This study investigates the growth tolerance mechanisms ofChlorella pyrenoidosato 3-fluorophenol and its removal efficiency by algal cells. Our results indicate thatC. pyrenoidosacan tolerate up to 100 mg/L of 3-fluorophenol, exhibiting a significant hormesis effect characterized by initial inhibition followed by promotion of growth. InC. pyrenoidosacells, the activities of superoxide dismutase (SOD) and catalase (CAT), as well as the levels of malondialdehyde (MDA) and reactive oxygen species (ROS), were higher than or comparable to the control group. Metabolic analysis revealed that the 3-fluorophenol treatment activated pathways, such as glycerol phospholipid metabolism, autophagy, glycosylphosphatidylinositol (GPI)-anchored protein biosynthesis, and phenylpropanoid biosynthesis, contributed to the stabilization of cell membrane structures and enhanced cell repair capacity. After 240 h of treatment, over 50% of 3-fluorophenol was removed by algal cells, primarily through adsorption. Thus,C. pyrenoidosashows potential as an effective biosorbent for the bioremediation of 3-fluorophenol.
研究领域:
环境科学
·
生物技术
·
微生物学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）