JOURNAL OF ETHNOPHARMACOLOGY
·
2025
·
IF 5.4
Shixiong Zhang,Xinjing Mao,Liping Chang,Mengnan Li,Cong Wei,Hongrong Li,Xiaogang Shen,Kunxu Niu,Runtao Zhang,Yuning Jiang,Xuan Lu,Yahui Song,Liangxing Zhou,Lini Gao,Zhiqin Zhao,Liying Niu,Qian Yang,Yunlong Hou,Yiling Wu
Ethnopharmacological relevance Bazi Bushen Capsule (BZBS), a traditional Chinese medicine formulation composed of multiple bioactive herbal components, has been validated in multicenter randomized double-blind controlled trials for its potent anti-aging properties. Previous studies from our group have demonstrated that BZBS effectively restores gut microbiota homeostasis and attenuates the impairment of intestinal barrier function, thereby ameliorating age-related cognitive decline. However, the specific molecular mechanisms by which BZBS modulates key microbial-metabolite networks to delay brain aging remain poorly understood and warrant further investigation. Aim of the study This study aims to elucidate the key microbiota-metabolite networks through which BZBS improves cognitive function and delays brain aging in senescence-accelerated mouse-prone 8 (SAMP8) mice. Materials and methods Eight-week-old male SAMP8 mice were used as experimental models, randomly divided into Model, BZ-low (0.5 g/kg/d BZBS), BZ-high (1 g/kg/d BZBS), and RAPA (2 mg/kg/d rapamycin) groups. Senescence-accelerated mouse resistant 1 (SAMR1) mice served as the control group. Cognitive function was assessed using the Barnes Maze test and the three-chamber social test. The structural damage and pathological changes in the brain tissue were evaluated through transcranial Doppler, micro-computed tomography, Nissl staining, and Western blot analysis. Next, the intestinal barrier function was detected by hematoxylin-eosin (HE) staining, periodic acid-Schiff (PAS) staining, and immunofluorescence (IF) staining. Characteristic bacteria were identified by 16S rRNA sequencing, and metabolomic profiling was performed using non-targeted metabolomics. Akkermansia muciniphila (Akk) was cultured, and fecal microbiota transplantation (FMT) was employed to evaluate its contribution to intestinal barrier function. Results The study revealed that BZBS therapy not only enhances cognitive capabilities but also restores the intestinal barrier function. Akk was identified as a key regulatory agent mediating the therapeutic effects of BZBS. BZBS administration significantly increased the abundance of Akk and modulated its metabolite profile, particularly components associated with spermidine, thereby reinforcing the intestinal barrier and mitigating age-related cognitive decline. Furthermore, this study demonstrated that Akk, administered via fecal microbiota transplantation, alleviated dextran sulfate sodium (DSS)-induced colitis.. Conclusion The results showed that BZBS capsule, a traditional Chinese medicine, may delay brain aging in SAMP8 mice by modulating Akk and its spermidine production.
研究领域:
民族药理学
·
微生物组学
·
代谢组学
·
神经科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Cell Reports
·
2025
·
IF 6.9
Zeng Zhang,Quan Guo,Zhihan Yang,Yukai Sun,Shuaiming Jiang,Yangli He,Jiahe Li,Jiachao Zhang
Sarcopenia significantly diminishes quality of life and increases mortality risk in older adults. While the connection between the gut microbiome and muscle health is recognized, the underlying mechanisms are poorly understood. In this study, shotgun metagenomics revealed that Bifidobacterium adolescentis is notably depleted in individuals with sarcopenia, correlating with reduced muscle mass and function. This finding was validated in aged mice. Metabolomics analysis identified nicotinic acid as a key metabolite produced by B. adolescentis , linked to improvements in muscle mass and functionality in individuals with sarcopenia. Mechanistically, nicotinic acid restores nicotinamide adenine dinucleotide (NAD+) levels in muscle, inhibits the FoxO3/Atrogin-1/Murf-1 axis, and promotes satellite cell proliferation, reducing muscle atrophy. Additionally, NAD+ activation enhances the silent-information-regulator 1 (SIRT1)/peroxisome-proliferator-activated-receptor-γ-coactivator 1-alpha (PGC-1α) axis, stimulating mitochondrial biogenesis and promoting oxidative metabolism in slow-twitch fibers, ultimately improving muscle function. Our findings suggest that B. adolescentis -derived nicotinic acid could be a promising therapeutic strategy for individuals with sarcopenia.
研究领域:
老年医学
·
微生物学
·
代谢组学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TISSUE & CELL
·
2025
·
IF 2.5
Xizhi Chen,Yao Chen,Weibo Wen
This study aimed to explore effectiveness and related mechanisms of Polygala crotalarioides in treating erectile dysfunction (ED). Hydrocortisone was used to establish a kidney- Yang deficiency ED rat model. Levels of triiodothyronine(T3), thyroxine(T4), testosterone(T), estradiol(E2) and expressions of RhoA, ROCK1, ROCK2 were determined. Immunofluorescence staining was used to detect CD34 in penile tissues. Compared with normal rats, T3, T4, T, E2 levels in serum of ED rat model were significantly reduced (P < 0.05), while RhoA, ROCK1, ROCK2 expressions were significantly higher (P < 0.05). High- and medium-dose of Polygala crotalarioides significantly attenuated erectile dysfunction, but low-doses of Polygala crotalarioides were ineffective. High and medium dose of Polygala crotalarioides and sildenafil significantly increased weight of ED rats compared to Model group (P < 0.05). T3, T4, E2, and T levels in low-dose, medium-dose, and high-dose of Polygala crotalarioides group were increased compared to Model group (P < 0.05). Compared with Model group, RhoA, ROCK1, and ROCK2 expressions in penile tissue of rats in low-, medium-, high-dose of Polygala crotalarioides group were reduced (P < 0.05). High-, medium-, and low-dose Polygala crotalarioides significantly increased CD34 compared with ED rat (P < 0.05). In conclusion, Polygala crotalarioides attenuated erectile dysfunction and improved thyroid and gonadal function by regulating Rho/ROCK pathway in kidney Yang- deficiency ED rat.
研究领域:
药理学
·
内分泌学
·
中医药学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
·
2016
·
IF 1.964
Xiaoyao Fei,Shaoyun Chen,Dai Liu,Chunjie Huang,Yongchun Zhang
Two functionalized SBA-15 [amine-functionalized SBA-15 (AFS) and epoxy-functionalized SBA-15 (GFS)] with different types of functional groups were synthesized by a hydrothermal process and post functionalized with 3-aminopropyltriethoxysilane (APTES) and 3-glycidyloxypropyltrimethoxysilane (GPTMS), respectively. They were used for the immobilization of carbonic anhydrase (CA). The physicochemical properties of the functionalized SBA-15 were characterized by X-ray powder diffraction (XRD), N 2 adsorption–desorption, 13 C, 29 Si solid-state nuclear magnetic resonance (NMR) spectroscopy, and scanning electron microscopy (SEM). Before and after CA was immobilized on AFS and GFS, the effects of temperature and pH value on the enzyme activity , storage stability, and reusability were investigated using para -nitrophenyl acetate ( p -NPA) assay. CA/GFS showed a better performance with respect to storage stability and reusability than CA/AFS. Moreover, the amount of CaCO 3 precipitated over CA/AFS was less than that precipitated over CA/GFS, which was almost equal to that precipitated over the free CA. The results indicate that the epoxy group is a more suitable functional group for covalent bonding with CA than the amino group, and GFS is a promising support for CA immobilization.
研究领域:
生物化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Chemistry-An Asian Journal
·
2022
·Vol. 17
·期 15
·e202200397
·
IF 4.839
Xi Yang,Jinchuang Ning,Yingying Zhao,Suying Xu,Leyu Wang
Graphical An amphiphilic fluorine-containing molecule CFO with high fluorine content (11.78 wt %) was synthesized, of which the hydrophilic sulfoxide and carboxyl groups resulted in 19 F MRI with high sensitivity, and the hydrophobic chain ensured surface functionalization of inorganic nanoparticles to afford fluorine labeling nanoparticles. The development of multifunctional nanoplatforms for bioimaging and therapy has attracted extensive attention in recent decades. As an emerging magnetic resonance imaging (MRI) technique, 19 F MRI effectively complements the limit of 1 H MRI owing to trace amount of 19 F atoms existing in the human body, affording high specificity. In this work, a novel molecule ( CFO ) containing hydrophilic fluorine fragments was synthesized using amino acids as starting materials, which could self-assemble into small nanoprobes via hydrophobic interactions of oleic acid moiety. The as-prepared nanoprobes displayed good relaxation performances thanks to the carboxylate group and sulfoxide group nearby. The nanoprobes successfully demonstrated its capacity for in-vivo 19 F MRI. In addition, CFO could be used as a general fluorine labeling agent for surface functionalization of nanoparticles, which provided a universal method for constructing water-soluble multifunctional nanoplatforms.
研究领域:
生物医学工程
·
分子影像学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PROGRESS IN ORGANIC COATINGS
·
2023
·
IF 6.6
Huijie Huangfu,Xugeng Guo,Ning Li,Ye Xiong,Yafei Huang,Jinglai Zhang,Li Wang
Smart coatings play crucial roles in realizing excellent corrosion protection on metals and alloys. However, the preparation of smart composite coatings exhibiting self-reporting and self-healing dual function still remains a challenge. Herein, a novel smart composite coating was successfully fabricated by incorporating the ionic liquid grafted carboxylated carbon nanotube and phenolphthalein into a micro-arc oxidation coating (MAO/PP/CCNT-IL) for AZ31B Mg alloy protection. The electrochemical tests including potentiodynamic polarization and electrochemical impedance spectroscopy reveal that the MAO/PP/CCNT-IL composite coating shows the best corrosion protection on the AZ31B Mg substrate among all coatings prepared in this work. In addition, the salt spray experiment in 5 wt% NaCl solution suggests that the occurrence of conspicuous pink within only 12 min can rapidly warn microdamage and microsurface damage, exhibiting a self-reporting effect. Meanwhile, the smart coating also presents outstanding healing property by reacting CCNT-IL with Mg ions from the substrates to generate the chelates to be covered on the damaged area. Such a smart coating offers a feasible strategy for prolonging the service lifetime of Mg alloys through a convenient, economical and effective way with a promising application in the field of material protection.
研究领域:
材料科学
·
腐蚀与防护
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2016
·
IF 3.014
Zihui Liang,Li Zhao,Wanfan Meng,Cheng Zhong,Shoubin Wei,Binghai Dong,Zuxun Xu,Li Wan,Shimin Wang
A simple and cost effective sol-gel process is developed for producing thermochromic tungsten-doped thin films of vanadium dioxide (VO 2 ). The precursor is first prepared by the reaction of vanadyl acetylacetonate, methanol, and tungsten chloride in a beaker. The precursor is then spin-coated on the substrate and, finally, annealed at 600 °C in argon gas. The resulting vanadium dioxide thin films are characterized by X-ray diffraction, X-ray photoelectron spectroscopy, scanning electron microscopy, variable temperature UV/Vis spectroscopy, and contact angle measurements. The results indicate that the particles of tungsten-doped vanadium dioxide thin films range from 30 to 150 nm, and the thin films show excellent hydrophilicity with a water contact angle (WCA) of 12°. According to optical tests, the tungsten-doped vanadium dioxide thin films exhibit satisfactory optical properties with an applicable integrated luminous transmittance ( T lum,s = 80.75%, T lum,m = 79.24%) and excellent solar regulation efficiency (△ T sol = 9.10%, from T sol,s = 81.40% to T sol,m = 72.30%). The VO 2 films exhibit an applicable transition temperature of the semiconductor-to-metal phase change: at a W-doping level of 2 at.%, the transition temperature was measured to be 32 °C. The simple and low-cost method is a noteworthy addition to literature on the synthesis of nanostructured materials towards applications in energy-saving smart windows.
研究领域:
材料科学
·
能源工程
✓ 已验证
DOI
高影响因子
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
·
2025
·
IF 4.5
Jinyao Liu,Yingying Pang,Wenyan Li,Juanjuan Sun,Yujie He,Yonghong Guo,Jing Dong
Linezolid, an oxazolidinone antibiotic, is used in patients with liver or kidney disease. However, the effects and mechanisms of hepatic impairment or renal failure on the pharmacokinetics of linezolid and its metabolites (PNU-142586 and PNU-142300) remain unclear. We used carbon tetrachloride-induced impaired hepatic function and 5/6 nephrectomy-induced renal failure rat models to investigate linezolid and metabolite pharmacokinetics. Isolated primary rat hepatocytes were used to evaluate the impact of hepatic impairment or renal failure on linezolid metabolism. Uptake and efflux transport studies were also conducted. The influence of hepatic impairment or renal failure on the pharmacokinetics of linezolid and two metabolites did not differ between intragastric gavage and intravenous administration in rats. Linezolid did not accumulate in the brain, heart, lung, liver, kidney, and small intestinal tissues of the hepatic impairment or renal failure rats. And PNU-142300 did not accumulate in the liver or kidney tissue. Compared to the isolated normal rat hepatocytes, the in vitro hepatic clearance of linezolid in hepatic impairment and renal failure rat hepatocytes decreased by 61.3% and 44.1%, respectively. Organic anion transporting polypeptide (OATP)1B1, OATP1B3, OATP2B1, Na+-taurocholate co-transporting polypeptide (NTCP), organic anion transporter (OAT)1, OAT3, multidrug resistance-associated protein 2 (MRP2), or multidrug resistance protein 1 (MDR) did not mediate linezolid transport. Hepatic impairment primarily increases linezolid exposure through reduced hepatic metabolism, whereas renal failure increases both linezolid and two metabolites exposure through reduced hepatic metabolism and renal glomerular filtration. These findings guide adjusting the dose of linezolid in patients with hepatic and renal insufficiency.
研究领域:
药理学
·
肝脏病学
·
肾脏病学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Polymer Materials
·
2025
·
IF 4.7
Weihan Tao,Xianglu Zhao,Yinchun Fang,Xianfeng Wang
Biomimetic construction of structural colors on materials as an environmentally friendly coloring method has received more and more attention. If the structural color of the materials has special functions, it will broaden the application field. However, functional structural colors are usually prepared through the coassembly of building units with functional agents or functional modification of structural colors. In this study, poly(2,2,3,4,4,4-hexafluorobutyl acrylate/methyl methacrylate/[3-(methyl acrylamide) propyl] trimethylammonium chloride) (PAMM) microspheres containing hydrophobic and antibacterial functional groups were prepared, which were used to construct multifunctional structural colors on black polyester fabrics through the atomization deposition method. PAMM microspheres with different particle sizes (332.1, 318.3, 302, 289.9, 257.2 nm) were successfully prepared by adjusting the amounts of monomers in the synthesis reaction, which can form different structural colors of pink, green, cyan, blue, and purple on the polyester fabrics. The fabrics with structural color can significantly inhibit the growth of E. coli and S. aureus, and the antibacterial rates of both are greater than 94%. The fabrics with structural color showed outstanding hydrophobicity with a static water contact angle greater than 140°. This research provides an idea for the preparation of multifunctional fabrics with structural colors through the synthesis of building units of polymer microspheres with multifunctional groups.
研究领域:
材料科学
·
纺织工程
·
生物医学
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Wentao Zhu,Fang-Yi Huo,Lei-Ming Cao,Zi-Zhan Li,Qian Zhu,Rongxiang He,Bolei Chen,Yong Liang,Bo Cai,Hong He,Lin-Lin Bu,Ligang Hu
Mimicking natural bio-surfaces with specific microstructures that possess inherent antibacterial ability provides a promising bacteriostatic strategy. However, current methods for biomimetic surface antisepsis can only be implemented on planar substrates made of limited materials. Herein, we developed a 3D printing-based strategy to facilely customize biomimetic microstructures on specified surfaces to successfully enable bacteriostatic function. Two-photon polymerization was employed to fabricate elaborate bacteriostatic microstructures on either flat or curved surfaces regardless of their material (daily-used glass or plastics). Streptococcus mutans were cultured on microstructured surfaces as model bacteria to validate surface bacteriostatic performance. As an example, the strategy was adopted to endow clear aligner substrate surface with bacteriostatic ability, which would enhance the applicability and convenience of these orthodontic devices for maintaining personal oral hygiene. Our strategy will inspire daily or industrial applications aiming at surface sterilization in a customized, convenient, and safe manner.
研究领域:
生物医学工程
·
材料科学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Neurology
·
2025
·
IF 2.8
Renlong Ji,Yanchang Xu,Kang Li,Wen Jiang,Yuan Li,Jianwei Zeng,Wei Li,Caiji Wang,Zeqi Zhao,Yalan Li,Naveena Konduru,Wen Liu,Yuehua Qiao,Xuanyi Li
Objective: To investigate the effects of Zexie decoction on vestibular and auditory function in guinea pigs with endolymphatic hydrolysis induced by desmopressin. Methods: Sixty guinea pigs were randomly and evenly divided into four groups, each group has 15 guinea pigs: normal control group, DDAVP group, DDAVP modeling combined with Zexie Decoction group, and DDAVP combined with Double Zexie group. At 7 and 14 days, bone-conducted cervical vestibular evoked myogenic potential tests, auditory brainstem responses, and distortion-product otoacoustic emissions were conducted on each group of guinea pigs to evaluate their vestibular and auditory function quantitatively. After functional testing, the outer hair cells were observed by scanning electron microscope. On day 14, one guinea pig was randomly selected from both the normal control group and DDAVP group to verify the successful establishment of the model using gadolinium-enhanced magnetic resonance imaging of the inner ear.Results: We conducted BC-cVEMP, ABR, and DPOAE tests on guinea pigs, and the results showed that DDAVP did affect vestibular function and hearing in guinea pigs. Analyses were performed from those results that were statistically significant, Zexie Decoction improved DDAVP-induced vestibular dysfunction and hearing loss dose-dependently, though complete reversal was not achieved. About scanning electron microscopy, outer hair cells of the DDAVP group showed significant loss and cilia lodging, however, treatment with Zexie decoction can alleviate the loss of outer hair cells and the lodging of cilia. When the outer hair cells were exposed to DDAVP for a long time, the improvement effect of Zexie decoction was not as obvious as before.Conclusion: The extent of improvement correlates with the concentration and dosage of Zexie Decoction. Even at double the dosage, Zexie Decoction only partially mitigates the decline in vestibular and auditory function induced by DDAVP, falling short of complete reversal.
研究领域:
药理学
·
听力学
·
神经科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Guangwei Shao,Wenlong Huang,Xin Zhang,Junjie Pan,Minghao Li,Hongjie Li,Renbiao Zhang,Bixia Sun,Zhihao Li,Qiusen He,Wenfeng Hu,Siyi Bi,Huiqi Shao,Jinhua Jiang,Nanliang Chen
The extremely cold environment restricts certain activities, making the preparation of a material with both heating and insulation functions a significant challenge. This study presents the large space warp-knitted composite fabric (LSWCF) as an electric heating inflatable material with a three-dimensional structure. It is designed and developed from three modes to mitigate heat loss. The top layer of LSWCF is combined with the electric heating film as the heating layer, showing high electric heating efficiency (577.33 °C/(W·cm–2)), high electrothermal conversion efficiency (95%), and excellent conductivity (12.5 S/cm). The bottom layer of LSWCF is coated with TiO2/WPU coatings as a thermal radiation layer. The inflated LSWCF retains a substantial volume of still air within its middle layer and exhibits low thermal conductivity (0.037 W·m–1·K–1), with resulting minimization of heat loss due to convection and conduction. LSWCF’s radiant coating, on the other hand, reduces heat loss through directed radiation. Contributed to the structural design, the LSWCF demonstrates an enhanced ability to generate heat at low temperatures (up to 35 °C at −20 °C, 980 W·m–2) compared to the existing literature. This study offers a novel strategy for the development of materials for use in extremely cold environments.
研究领域:
材料科学
·
纺织工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）