FOOD RESEARCH INTERNATIONAL
·
2023
·
IF 8.1
Jinrui Zhu,Li Zhou,Minjie Zhao,Fang Wei,Haiyan Fu,Eric Marchioni
Coffee is popular worldwide and its consumption is increasing in recent years. Although mass spectrometry-based lipidomics approaches have been prevalent, their application in studies related to detailed information and dynamic changes in lipid composition during coffee bean roasting is still limited. The aim of this study was to investigate the dynamic changes in coffee bean lipids during the roasting process. The lipid classes and lipid molecular species in coffee beans were characterized by lipidomic analysis combined with chemometrics. A total of 12 lipid classes and 105 lipid molecular species were identified and quantified. Triacylglycerols (TAG) was the most abundant lipid class in both green beans and roasted beans. The content of phosphatidylethanolamine (PE) and lysophosphatidylethanolamine (LPE) in green beans was obviously higher than that in roasted beans. Other phospholipids , such as phosphatidylinositol (PI), lysophosphatidylinositol (LPI), phosphatidylcholine (PC), lysophophatidylcholine (LPC) and phosphatidic acid (PA), showed a tendency to increase at the beginning of roasting, then decreased gradually. Several differential lipid molecule species, for instance, PE (16:0_18:2), PC (18:2_18:2) were significantly down-regulated, and PI (18:1_18:2) was significantly up-regulated. This study provided a scientific basis for the change of coffee bean lipids during the roasting process.
研究领域:
食品科学
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分析化学
·
植物代谢
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产品关联
DOI
PubMed
高影响因子
Food Chemistry-X
·
2025
·
IF 8.2
Jing Wang,Yuemeng Hu,Zhenyu Guan,Ronggang Zhai,Jieyao Yu,Marina Rigling,Yanyan Zhang,Xiaochun Wan,Xiaoting Zhai
Large-leaf yellow tea (LYT) is a yellow tea product with a specific aroma characteristic and the flavor code has recently been revealed. To elucidate the transformation of odorants from fresh leaves to finished LYT, this study tracked dynamic changes in aroma compounds throughout an industrial-scale experiment. The odorants' contributions of each step were evaluated by stable isotope dilution assays, internal standard method, and odor activity values. Among 39 quantitated odorants, the loss of top-note compounds such as dimethyl sulfide, 2-/3-methylbutanal, and 2-methylpropanal during yellowing was compensated during roasting. In contrast, floral compounds like geraniol and linalool declined progressively, while pyrazines were mainly generated during the final roasting and increased with roasting intensity. Therefore, yellowing and roasting steps were identified as key processes influencing LYT aroma formation, and the roasting degree significantly influenced the final aroma profile. These findings provide a mechanistic understanding of aroma development in LYT and offer practical guidance for optimizing processing parameters to tailor flavor profiles.
研究领域:
食品科学
·
茶叶化学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2018
·Vol. 54
·期 2
·490-498
·
IF 2.383
Zhou-Tao Fang,Chu-Jun Song,Hai-Rong Xu,Jian-Hui Ye
Summary Flavonol glycosides with low thresholds are important flavour substances in tea leaves. This study monitored the dynamic changes in flavonol glycosides during the production of different teas. The fresh leaves of cv. Fudingdabaicha with 15.77 mg g −1 of total flavonol glycosides (TFG) were respectively processed into green, yellow, white, oolong and black teas. A reduction of TFG was observed for different teas, amongst which black tea contained the lowest level of TFG (9.83 mg g −1 ), followed by oolong tea (11.96 mg g −1 ), while green, yellow and white teas contained similar levels of TFG (~13.70 mg g −1 ). Fixation was crucial to maintain the stability of flavonol glycosides due to its inactivation effect on enzymes at high temperature. Principal component analysis results indicated that flavonol glycosides were vulnerable to fermentation, and the stabilities of flavonol glycosides were related with aglycone and glycoside moiety.
研究领域:
植物化学
·
食品科学
·
茶叶加工学
✓ 已验证
产品关联
DOI
高影响因子
Arabian Journal of Chemistry
·
2021
·
IF 5.165
Danni Wang,Jiaming Guo,Xin Chai,Jing Yang,Yuefei Wang,Xiumei Gao
Fructus Psoraleae (FP), the dried ripe fruit of Psoralea corylifolia L., is a popular herbal medicine commonly applied for alleviating osteoporosis and vitiligo. But, until now, the dynamic variations of compounds in P. corylifolia have been less investigated during its growth, storage, and treatment by different temperatures, which is meaningful for guaranteeing the quality of FP. In this study, focused on these questions, with emphasis on the enzyme-driven dynamic transformation of coumarins, ultra-high performance liquid chromatography coupled with photodiode array detector (UHPLC-PDA) method was successfully established for the simultaneous determination of nine compounds. The distribution and accumulation of compounds were discussed and illuminated in different parts of P. corylifolia and samples harvested at different times. The characteristics of compounds' variation in flowers and fruits of P. corylifolia were identified. Through the market survey and quantitative study on FP, positive correlation was speculated between transformation from (iso)psoralenoside to (iso)psoralen via β -glucosidase and storage time, which was further confirmed by accelerated stability test. The effect of treated temperatures (40–210 °C) was unveiled on the enzyme activity and transformation from (iso)psoralenoside to (iso)psoralen in FP. And the focused compounds' transformation was mainly driven by β -glucosidase when the temperature was below 120 °C. Above 120 °C, β -glucosidase was completely inactivated, and the focused compounds' transformation was mediated by high-temperature, also the obvious degradation was found. Our results demonstrated that compounds' transformation characteristics arising from the growth, processing and storage of P. corylifolia are critical factors to ensure the quality of FP.
研究领域:
中药学
·
植物化学
✓ 已验证
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Jingna Li,Wei Zhang,Yaming Sun,Chenchen Song,Hailiang Zhao,Yangyong Lv,Yuansen Hu,Shusheng Zhang,Lijun He
Early warning detection based on aflatoxin B 1 (AFB 1 ) biosynthesis precursors is an effective strategy for the prevention of AFB 1 contamination. However, the development of effective methods for accurately tracking the dynamic changes of AFB 1 and its precursors presents a major challenge. This difficulty is due to the continual variations in precursor species and concentrations, structural differences of AFB 1 and its precursors, as well as the complexity of the dynamically changed sample matrix. Herein, an ionic liquid-impregnated covalent triazine polymer composite (IL@CTP) was fabricated, which exhibited excellent extraction performance for structurally different AFB 1 and its three typical precursors in moldy grains. The high performance was attributed to the multiple interactions between the prepared composite and analytes, as well as a mesopore filling effect. The combined use of IL@CTP with dispersive solid-phase extraction and ultra high-performance liquid chromatography-mass spectrometry achieved negligible matrix effects, with low limits of detection (0.01–0.03 ng/g), wide linearity (0.06–200 ng/g) and satisfactory relative recoveries of 85.3 %-113.0 %. Therefore, the developed method can accurately track the dynamic changes of AFB 1 and its precursors in stored maize inoculated with Aspergillus flavus . Correlation analysis between AFB 1 and its precursors showed that it was necessary to simultaneously serve averantin, O-methylsterigmatocystin and sterigmatocystin as early warning molecules, allowing the accurate identification of AFB 1 contamination risks up to 5 days in advance.
研究领域:
食品安全
·
分析化学
·
真菌学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2020
·
IF 5.977
Tingting Niu,Guoxing Yin,Ting Yu,Yabing Gan,Chenye Zhang,Juanjuan Chen,Wei Wu,Haimin Chen,Haitao Li,Peng Yin
Monitoring cellular GSH dynamics is of great value to understand its complex biological functions and related diseases. It still remains challenging to understand the mechanism of GSH dynamics in complex intracellular environment. Herein, a novel fluorescent probe featured with chlorinated coumarin-TCF was exploited for sensing of GSH with high selectivity and high sensitivity. Large fluorescence enhancement at 471 nm was observed upon addition of GSH with large emission distance ( Δλ em = 219 nm). This novel probe was successfully applied to monitor endogenous and exogenous GSH dynamics without interference of other thiols via fluorescence imaging. More importantly, using this probe, low dose reactive oxygen species induced GSH increasement through nuclear factor (erythroid-derived 2)-like 2 (Nrf2) signal pathway in BEL-7402 cells was observed. This novel fluorescent probe has the potential for quantitative monitoring of cellular GSH, which will promote further understanding of its physiological and pathological roles in biological systems.
研究领域:
生物化学
·
分子生物学
·
细胞生物学
✓ 已验证
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Ting Huang,Yinggen Zhang,Xiuping Wang,Hui Zhang,Changsong Chen,Quanbin Chen,Qiusheng Zhong
Ming guan’(MG), an elite albino cultivar deriving from the progeny of the traditional albino cultivar ‘Bai jiguan’, is a promising candidate for white tea production due to its favorable amino acid to phenol ratio. In this study, a comprehensive metabolomics analysis using ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) and headspace solid-phase microextraction-gas chromatography mass spectrometry (HS-SPME-GC–MS) were conducted to reveal the dynamic changes of non-volatile and volatile organic compounds (VOCs) throughout the withering processing of MG white tea. Meanwhile, multivariate statistical analyses were applied to screen for the characteristic components in the flavor and aroma of MG white tea. A total of 625 non-volatile metabolites and 118 VOCs were determined, of which 90 non-volatile metabolites (VIP ≥ 1, FC ≥ 2 or ≤ 0.5) were identified as key flavor components significantly changed throughout the withering process. The relative odor activity value (ROAV) analysis highlighted 22 VOCs (ROAV ≥ 1) with substantial effect on aroma formation, of which geraniol, (E)-2-hexenal, 4-methoxy-benzaldehyde and guaiacol emerging as the most key aroma constituents of MG white tea, endowing MG white tea with fruity and floral odor notes. This study offered a comprehensive investigation into metabolite changes in MG white tea, contributing valuable insights for the innovation of new white tea products utilizing albino tea plant mutants.
研究领域:
植物学
·
食品科学
·
代谢组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Translational Pediatrics
·
2021
·Vol. 10
·期 9
·2313
·
IF 2.488
Wei Shen,Wen Qiu,Yuting Liu,Weihua Liao,Yiyi Ma,Yan He,Zhang Wang,Hongwei Zhou
Background The gut microbiome plays a potential role in clinical events in preterm infants and may affect their lateral development. Understanding the initial colonization of microbes in the gut, their early dynamic changes, and the major factors correlated with these changes would provide crucial information about the developmental process in early life. Methods The present study enrolled 151 preterm infants and examined the longitudinal dynamics of their fecal microbiome profiles during the period of hospitalization using 16S ribosomal RNA gene sequencing. Random forest modeling was used to predict postnatal age (Age), postmenstrual age (PMA), and gestational age (GA), using gut microbiome features. Results Principal coordinate analysis revealed that the gut microbiome of the preterm infants displayed an obvious time-dependent change pattern, which showed the strongest association with Age, followed by PMA, and a much weaker association with (GA). Random forest modeling further evidenced the time-dependent change pattern, with the Pearson’s correlation coefficients between the actual values and the gut microbiome-predicted values being 0.68, 0.53, and 0.38 for postnatal, postmenstrual, and gestational age, respectively. The microbiome dynamism could be further divided into four Age stages, each with its own characteristic microbial taxa. The first 1–4 days (T1 stage) represented the meconium microbiome, with colonization of a high diversity of microbes before or during delivery. During 5–15 days (T2 stage), the gut microbiome of the preterm infants underwent a rapid turnover, in which microbial diversity declined, and stabilized afterward. Enterobacteriaceae , Enterococcaceae , Streptococcaceae , Staphylococcaceae , and Clostridiaceae were the major classes in the gut microbiome in the lateral stages of development (T3–T4 stage). Conclusions Postnatal age, rather than the gestational age, is significantly correlated with the gut microbiome of preterm infants, suggesting that clinical interventions contribute more to the early dynamics of gut microbiome in preterm infants than the natural development of the gut.
研究领域:
微生物组学
·
新生儿科学
·
发育生物学
·
临床微生物学
·
生物信息学
✓ 已验证
DOI
PubMed
高影响因子
Plants-Basel
·
2024
·Vol. 13
·期 9
·1249
·
IF 4
Mengcong Zhang,Lixuan Zhang,Chengzhe Zhou,Kai Xu,Guangwu Chen,Linjie Huang,Zhongxiong Lai,Yuqiong Guo
The enzymatic reaction stage (ECS) of oolong tea processing plays an important role in the formation of the flavor quality of the oolong tea. To investigate the dynamic changes in the volatile and non-volatile components in the leaves of oolong tea during the ECS, metabolomic studies were carried out using the leaf samples collected at different stages of the ECS of Aijiao oolong tea. Out of the identified 306 non-volatile metabolites and 85 volatile metabolites, 159 non-volatile metabolites and 42 volatile metabolites were screened out as key differential metabolites for dynamic changes during the ECS. A multivariate statistical analysis on the key differential metabolites showed that the accumulations of most metabolites exhibited dynamic changes, while some amino acids, nucleosides, and organic acids accumulated significantly after turning-over treatment. The evolution characteristics of 27 key precursors or transformed VOCs during the ECS of Aijiao oolong tea were clarified, and it was found that the synthesis of aroma substances was mainly concentrated in lipids as precursors and glycosides as precursor pathways. The results revealed the dynamic changes in the flavor metabolites in the ECS during the processing of Aijiao oolong tea, which provided valuable information for the formation of the characteristic flavor of Aijiao oolong tea.
研究领域:
食品科学
·
茶叶加工
·
代谢组学
·
风味化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2021
·
IF 3.935
Ji-shuo Chang,Zhong-xiao Lin,Ya-juan Liu,Si-ming Yang,Yu Zhang,Xi-yong Yu
Previous studies have reported that nucleic acid methylation is a critical element in cardiovascular disease, and most studies mainly focused on sequencing and biochemical research. Here we developed an Ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/ MS) method for the quantification analysis of the dissociative epigenetic modified nucleosides (5mdC, 5mrC, m 6 A) in Myocardial Infarction (MI) SD rats from different periods (1 week, 4 weeks, 8 weeks) after the surgery. The samples for analysis were obtained from heart tissue and blood of the rats. All the quantification results are compared with the sham-operated group. Total RNA and DNA were isolated by enzymatic hydrolytic methods before the UPLC-MS/MS analysis. The statistical analysis demonstrates the dynamic changes of modified nucleosides in MI rats, and it showed good specificity, accuracy, stability and less samples were needed in the method. In this paper, we discovered that the concentration of 5mdC, 5mrC, m 6 A from heart tissue significantly increased at 8 weeks after the surgery. Furthermore, UPLC-MS/MS helps us observe the similar change of the concentration of those 3 methylated biomarkers in peripheral blood after 8 weeks. The result shows that the dynamic process of those 3 methylated biomarkers in peripheral blood is related to the content of methylated biomarkers from the heart tissue. Based on the scientific evidence available, we proved that the methylation of genetic materials in peripheral blood is similar to myocardial infarction tissue. The relation between them indicates that peripheral blood could be a promising alternative to the heart tissue which monitor the level of methylation and MI diagnosis-aided.
研究领域:
心血管疾病
·
表观遗传学
·
分析化学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2024
·
IF 2.7
Yang Liu,Linfeng Sheng,Muhammad Abdullah,Xing Shen,Ying He,Juan Liu,Xin Chen
In situ liquid cell electron microscopy technology has high temporal and spatial resolution, enabling real-time observation of physical and chemical processes in liquid environments. However, the common in situ liquid cell scanning electron microscopy (LC-SEM) technology has faced limitations due to the relatively large thickness of the SiNx window, resulting in unsatisfactory resolution, limiting the broad adoption of the LC-SEM technology. This article represents one novel approach of utilizing ultra-thin SiNx windows for LC-SEM observation of the distribution and dynamic changes of gold (Au) nanoparticles/zinc oxide quantum dots (ZnO QDs)/reduced graphene oxide (rGO) ternary composites in liquid. The results demonstrate a single/near-single dispersed distribution of ZnO QDs on the surface of the rGO, with Au nanoparticles and ZnO clusters mainly concentrated on the rGO folds. The composite structure demonstrates relative stability, attributed to electrostatic forces. Using Au nanoparticles and ZnO QDs as hierarchical reference markers, a high LC-SEM image resolution of ~4 nm is obtained, which allows the observation and motion analysis of the Au nanoparticles, rGO nanosheets, as well as ZnO QDs in the liquid cell. Such LC-SEM technology shows good repeatability on the noble-metal/ZnO-QDs/rGO ternary composite samples, with good potential for the future QD composite material researches.
研究领域:
材料科学
·
纳米技术
·
电子显微镜
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Wanxing Zheng,Yufei Tang,Rui Yang,Zhangwen Xie,Yani Sun,Kang Zhao
Effectively attenuating highly penetrative low-frequency noise (LFN, f < 500 Hz) presents a significant challenge. Traditional acoustic insulation materials are circumscribed by the mass law, presenting an inherent conflict between lightweight design and acoustic insulation. Despite the promising potential of PUA aerogels in acoustic insulation, attributed to their lightweight and high damping properties, their low-density results in inadequate areal density. Consequently, it remains challenging to effectively suppress LFN through mass inertia alone. Moreover, lightweight design often reduces mechanical properties, creating a conflict between lightweight, high strength, and low-frequency acoustic insulation. Inspired by the periodic structures of metamaterials, this study fabricates a PUA aerogel with an ordered metamaterial-like structure (density: 0.126 g/cm 3 ). Through self-assembly induced by sodium dodecyl sulfate (SDS), an ordered porous cavity and fibrous topological skeleton were formed. This structure creates an efficient interfacial reflection network that provides dynamic acoustic impedance for LFN attenuation. The average STL is at least 42.6 dB in the 45–500 Hz range and 39.6 dB in the broader 45–6300 Hz range, 16.35 times higher than that of commercial melamine foam of the same 2 cm thickness. Its weight is just 1/770 that of lightweight concrete bricks with equivalent noise reduction performance, and its thickness is only 2/25 of theirs. At 145 °C, the aerogel maintains a temperature difference of approximately 105.05 °C between its surfaces. The compressive strength of the modified aerogels is 2.76 times that of unmodified PUA aerogels. The simple, cost-effective fabrication process and the lightweight, high-strength, and outstanding low-frequency acoustic insulation properties make metamaterial-like PUA aerogels promising for noise reduction in residential, transportation, and industrial applications.
研究领域:
材料科学
·
声学超材料
·
噪声控制工程
·
聚合物纳米复合材料
·
可持续建筑材料
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）