ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2015
·Vol. 407
·期 24
·7359-7368
·
IF 3.436
Zhao Zhiyong,Liu Na,Yang Lingchen,Deng Yifeng,Wang Jianhua,Song Suquan,Lin Shanhai,Wu Aibo,Zhou Zhenlei,Hou Jiafa
Mycotoxins have the potential to enter the human food chain through carry-over of contaminants from feed into animal-derived products. The objective of the study was to develop a reliable and sensitive method for the analysis of 30 mycotoxins in animal feed and animal-derived food (meat, edible animal tissues, and milk) using liquid chromatography–tandem mass spectrometry (LC–MS/MS). In the study, three extraction procedures, as well as various cleanup procedures, were evaluated to select the most suitable sample preparation procedure for different sample matrices. In addition, timed and highly selective reaction monitoring on LC–MS/MS was used to filter out isobaric matrix interferences. The performance characteristics (linearity, sensitivity, recovery, precision, and specificity) of the method were determined according to Commission Decision 2002/657/EC and 401/2006/EC. The established method was successfully applied to screening of mycotoxins in animal feed and animal-derived food. The results indicated that mycotoxin contamination in feed directly influenced the presence of mycotoxin in animal-derived food.
Multi-mycotoxin analysis of animal feed and animal-derived food using LC-MS/MS
研究领域:
食品安全
·
分析化学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Jinxia Dai,Hui Lin,Yuning Pan,Yanmin Sun,Ye Wang,Jun-qin Qiao,Hong-zhen Lian,Chun-xiang Xu
Chlorpromazine (CPZ) is abused in animal husbandry and can be extensively metabolized in humans and animals. However, the actual monitoring mainly focuses on the parent compound but lacks attention to its metabolites. A method was developed and validated firstly for identification and determination of CPZ and its four major metabolites in animal-derived foods using ultrahigh-performance liquid chromatography coupled with quadrupole-Orbitrap mass spectrometry in combination with QuEChERS preparation method. Satisfactory recoveries of analytes spiked in fish and pork samples ranged from 72 to 117 %, and limits of quantification were 2.0 and 1.0 μg kg −1 for fish and pork samples respectively. Moreover, through the hydrolysis experiments of CPZ, its hydrolysates, such as CPZ-sulfoxide, CPZ- N -oxide and CPZ-sulfoxide- N -oxide, were identified as potential risk compounds. The developed method has been successfully applied to the determination of CPZ and its metabolites in actual commercial samples, as well as to the screening of other CPZ-related risk compounds.
研究领域:
食品安全
·
分析化学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Wei Wei,Dan Li,Chenyu Jiang,Xinghe Zhang,Xue Zhang,Qingzhe Jin,Xi Zhang,Xingguo Wang
We compared phospholipids (PLs) content, their molecular species, and milk fat globules size and microstructure in the milk of five mammalian species, including human, cow, goat, yak, and donkey. The absolute quantification of major PLs was determined using 31 P NMR and their fatty acid composition with GC. The molecular species of PLs were analysed using LC-MS where a total of 9 PL species, including one sphingomyelin (SM), six glycerophospholipid (GPL), and two lysoglycerophospholipids (lyso-GPLs), were identified. PLs profile shows an obvious difference among the species, with human milk showing higher SM content and more unsaturated fatty acyls than other mammalian milk. The mammalian milk show a similar core-membrane lipid structure but obvious different size distribution. These data provide a basis for better construction of infant formulas to provide PLs requirements and a similar milk fat globule structure for infants.
研究领域:
营养学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
·
2018
·
IF 0.824
Ran LI,Ze-Jia LIN,Jin-Yi YANG,Zhen-Lin XU,Hong WANG,Hong-Tao LEI,Yuan-Ming SUN,Yu-Dong SHEN
A high-affinity polyclonal antibody was prepared by immunizing animals with haptens FFD and FFM. Under the optimal combination of coating antigen and antibody, an indirect competitive enzyme-linked immunosorbent assay (icELISA) for simultaneous detection of florfenicol and thiamphenicol residues in animal meat and urine samples was developed. The icELISA showed an IC 50 value of 1.32 ng mL −1 for florfenicol and 2.13 ng mL −1 for thiamphenicol, respectively. The linear ranges were from 0.31 to 5.61 ng mL −1 with a limit of detection of 0.12 ng mL −1 for florfenicol, and 0.41 to 11.2 ng mL −1 with a limit of detection of 0.15 ng mL −1 for thiamphenicol, respectively. The average recoveries of florfenicol and thiamphenicol in spiked samples ranged from 77.2% to 116.0% with a relative standard deviation of less than 15%. Therefore, this proposed icELISA provided a valid detection method for florfenicol and thiamphenicol residues in animal tissue and urine samples.
研究领域:
免疫学
·
食品安全检测
✓ 已验证
DOI
高影响因子
Foods
·
2024
·Vol. 13
·期 10
·1478
·
IF 4.7
Bingyu Zheng,Yida Wu,Yong Wang,Ying Li
Inspired by the proved dissolving power of vegetable oils for non-polar and low-polar natural compounds, animal fats with triglycerides as the major components were investigated as food-grade solvents in this study for the simultaneous extraction of carotenoids and capsaicinoids from Sichuan chili. The dissolving power of lard, beef tallow, chicken fat and basa fish oil in the extraction of er jing tiao chili was firstly compared, where animal oils with worse extraction ratios for carotenoids (0.79 mg/g in average) performed better for the extraction of capsaicinoids (0.65 mg/g in average). Furthermore, the solvent effect of animal fats on the oleo-extracts was evaluated in terms of fatty acid composition, oil quality indexes, crystal polymorphism, melting and crystallization behaviors, where no significant differences were observed between animal fats before and after extraction. The oxidative stability of animal fats could be 1.02- up to 2.73-fold enhanced after extraction and the pungency degree could reach the same spicy level as commercial hotpot oil. In addition, the Hansen solubility parameters of solvents and solutes were predicted for further theoretical miscibility study, which helps to make a better comprehension of the dissolving mechanism behind such oleo-extraction. Overall, animal fats demonstrated their considerable solvent power for extracting carotenoids and capsaicinoids simultaneously from Sichuan chili, which showed significant potential for developing a novel Sichuan spicy hotpot oil with enhanced flavor and stability.
研究领域:
食品科学
·
化学工程
·
营养学
✓ 已验证
DOI
高影响因子
Innovative Food Science & Emerging Technologies
·
2023
·
IF 6.6
Muhammad Hussain,Yue Sun,Yue Pan,Lu Liu,Xiuxiu Zhang,Qi Wang,Shuang Lin,Abdul Qayum,Kifayat Hussain,Xiaodong Li
Fatty acids content in walnut oil was investigated and compared with human milk, infant formula (IF) and animal milk (AM), then subjected to in-vitro digestion. Walnut oil (80.04 mg/g) and Human milk (73.11 mg/g) were comprised of unsaturated fatty acids , while AM (52.23 mg/g) and IF (40.50 mg/g) were significantly lower in unsaturated fatty acids. Confocal micrographs and particle size showed complete digestion of walnut oil-based infant formula (IFW) than AM and IF. Total fatty acid released during in-vitro digestion in different samples was IFW (2563.26 μmol/g fat), HM (2420.38 μmol/g fat), AM (1863.70 μmol/g), and in IF (3001.20 μmol/g). In IFW and HM, C18:2n6c was the highest single fatty acid released, with 47% and 40%, respectively, which was substantially different from AM and IF. Our research findings might help design an infant formula closer to human milk.
研究领域:
营养学
·
食品科学
·
儿科
✓ 已验证
DOI
高影响因子
Journal of Cleaner Production
·
2024
·
IF 9.7
Jiajun Pan,Zhaopeng Xia,Haibao Zhang,Yang Lu,Yong Liu
Dark animal fiber is bleached to satisfy the demand of color diversity for clothing. Comparing with frequently-used Fe 2+ catalyzed H 2 O 2 (Fe 2+ /H 2 O 2 ) bleaching method, the ammonium persulfate (APS)/H 2 O 2 bleaching method based on the persulfate (PDS)-based advanced oxidation process is considered to be an environmentally animal fiber bleaching method, which is advantageous in high efficiency, heavy metal-free and low damage. However, the degradation mechanism of eumelanin of APS/H 2 O 2 bleaching method remains to be explored. Herein, standard eumelanin (SM) which has higher purity of eumelanin was used as reaction model, and SM was proved to degrade into five soluble oligomers by chemical structure analysis. Therefore, a new degradation pathway of eumelanin in APS/H 2 O 2 solution was simulated and proposed by in vitro experiment. In addition, the yak wool fiber was low damage by testing the fiber structure before and after bleaching. The dyeing property of yak wool fabrics had no effect on APS/H 2 O 2 bleaching. This investigation of degradation products provides new insights into the bleaching mechanism of APS/H 2 O 2 environmentally dark animal fiber bleaching method.
研究领域:
化学
·
环境科学
·
纺织科学
✓ 已验证
产品关联
DOI
高影响因子
Scientific Reports
·
2024
·Vol. 14
·期 1
·1-9
·
IF 3.8
Sun Yunze,Mi Zhuang,Chen Xiaoyu,Li Jian-Jun,Lu Jun,Shan Xinyan,Lu Xinghua,Du Yuguang
Glycans, nucleic acids and proteins are three major classes of natural biopolymers. The extremely high diversity of isomerization makes structural elucidation of glycans the most challenging job among three classes. In the past few years, the single molecule sensing technique based on nanopores has achieved great success in sequencing of DNA. Inspired by this, it is potential to sequence glycans in the similar manner. Herein, SiN x nanopores were used to identify nine common monosaccharides in mammals. Each monosaccharide showed characteristic blockage current, which roughly increased with the increase of its molecular weight. In order to distinguish nine monosaccharides, several machine learning models were trained and tested, of which the highest F1 value was 1. These results illustrated that nine common monosaccharides in mammals could be clearly identified and discriminate by our method combining solid-state nanopores and machine learning. As far as we know, this is the first report that monosaccharides can be sensed and distinguished by solid-state nanopores. Our work showed the great potential of solid-state nanopores in glycan sequencing, and would lay the foundation for solid-state nanopore-based glycan sequencing.
研究领域:
生物化学
·
分子生物学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Scientific Reports
·
2025
·Vol. 15
·期 1
·1-15
·
IF 3.9
Huang Minyi,Zhang Yuhao,Xu Xiang,Duan Renyan,Yang Hui
Chlorothalonil is a widely used fungicide that has a negative effect on individual movement, but its impact pathway needs further refinement. Here, the effects of exposure to chlorothalonil on the locomotion behavior of Pelophylax nigromaculatus tadpoles (GS23) were measured at three different levels (0 µg/L, 10 µg/L, and 50 µg/L), and the possible pathways of its effects were analyzed from the gut-liver axis. Chlorothalonil exposure levels of 10 µg/L and 50 µg/L significantly reduced the average speed of P. nigromaculatus tadpoles by 26% and 32.7%, respectively, and significantly decreased the locomotor frequency by 27.1% and 58.6%, respectively. Gut microbiota analysis revealed chlorothalonil exposure significantly increased the abundance of Firmicutes , while significantly decreased the abundance of Actinobacteriota , Pseudomonas , and Rhodococcus . Metabolomics analysis identified that chlorothalonil treatment changed amino acid-related metabolism pathways in the gut and liver and altered the glycerophospholipid metabolism pathway in the liver. This study indicated that chlorothalonil can affect individual locomotor abilities and interfering with the gut-liver axis of aquatic animals. These findings establish that chlorothalonil compromises aquatic organism motility through a multi-target mechanism involving gut microbiota modulation, amino acid metabolic interference, and hepatic lipid pathway disruption.
研究领域:
生态毒理学
·
微生物组学
·
代谢组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2014
·
IF 5.825
Jing Li,Laizhong Chen,Wenxiao Wu,Wei Zhang,Zhao Ma,Yanna Cheng,Lupei Du,Minyong Li
To find an approach that can image the hydrolysis activity of aminopeptidase N (APN) both in vitro and in vivo, three bioluminescent probes have been well designed and synthesized herein. All of them can be recognized and hydrolyzed by APN to produce bioluminescence emission in the presence of firefly luciferase. To the best of our knowledge, they are the first bioluminescent probes for imaging APN in deep tissues and living animals.
研究领域:
生物化学
·
分子影像学
·
医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2024
·
IF 12.2
Shuyan Xu,Pengfeng Zhu,Caiqin Wang,Daoyong Zhang,Ming Zhang,Xiangliang Pan
The behavior and fate of PFOS (perfluorooctanesulfonate) in the aquatic environment have received great attention due to its high toxicity and persistence. The nanoscale supramolecular mechanisms of interaction between PFOS and ubiquitous EPS (exopolymers) remain unclear though EPS have been widely-known to influence the bioavailability of PFOS. Typically, the exposure patterns of PFOS in aquatic animals changed with the EPS-PFOS interaction are not fully understood. This study hypothesized that PFOS exposure and accumulation pathways depended on the PFOS-EPS interactive assembly behavior and animal species. Two model animals, zebrafish and chironomid larvae, with different feeding habitats were chosen for the exposure and accumulation tests at the environmental concentrations of PFOS in the absence and presence of EPS. It was found that PFOS triggered the self-assembly of EPS to form large aggregates which significantly trapped PFOS. PFOS accumulation was significantly promoted in zebrafish but drastically reduced in chironomid larvae because of the nanoscale interactive assembly between EPS and PFOS. The decreased dermal uptake but increased oral uptake of PFOS by zebrafish with large mouthpart size could be ascribed to the increased ingestion of PFOS-enriched EPS aggregates as food. For the chironomid larvae with small mouthpart size, the PFOS-EPS assemblies reduced the dermal, oral and intestinal uptake of PFOS. The nano-visualization evidences confirmed that the PFOS-enriched EPS-PFOS assemblies blocked PFOS penetration through skin of both animals. These findings provide novel knowledge about the ecological risk of PFOS in aquatic environments.
研究领域:
环境科学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL POLLUTION
·
2021
·
IF 8.071
Wantong Tang,Guiyin Wang,Shirong Zhang,Ting Li,Xiaoxun Xu,Ouping Deng,Ling Luo,Yan He,Wei Zhou
Rare earth elements inevitably release into the soil due to their widespread application. However, it is unclear how they affect the soil animals. The study surveyed the growth and physiological responses of earthworm ( Eisenia fetida ) exposed into artificial soils spiked with La , Ce, and their mixture, and actual mine soil collected from an abandoned La–Ce mining area (Mianning, Sichuan). The results showed that the 1000–1200 mg/kg combined exposure in two soils induced significant histopathological and phenotypic changes of earthworms. Concentration significantly affected the superoxide dismutase (SOD), peroxidase (POD), malondialdehyde (MDA), and protein of E. fetida and the effects differentiated with the prolonging duration. These indicators were negatively affected under the La stress ≥800 mg/kg (SOD, POD, and protein), the 1200 mg/kg (SOD), Ce stress ≥1000 mg/kg (protein), and the combination ≥800 mg/kg (SOD, POD) and ≥1000 mg/kg (protein). Artificial combination had −15.04% (SOD), 8.87% (POD), 5.64% (MDA), and −8.34% (protein) difference compared with the contamination soil, respectively. Overall, E. fetida respond sensitively under the La and Ce stress, the antioxidant defense system and the lipid peroxidation were stimulated, and the artificial soil might overestimate eco-toxicological effect.
研究领域:
环境科学
·
生态毒理学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）