ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Shuangtian Dong,Dongyang Wang,Xuwen Gao,Li Fu,Jingna Jia,Yuqi Xu,Bin Zhang,Guizheng Zou
All commercial chemiluminescence (CL) assays are conducted with either glow or flash CL of eye-visible waveband from chemical luminophores. Herein, glow and flash, as well as waveband adjustable CL from the same nanoparticle luminophore of thiol-capped CuInS2@ZnS nanocrystals (CIS@ZnS-Thiol), are proposed via extensively exploiting the differed redox nature of CL triggering reagents. Taking thiosalicylic acid (TSA) as the model thiol-capping agent, the electron-injection-initiated charge transfer between CIS@ZnS-TSA and reductant can bring out efficient glow CL while the hole-injection-initiated charge transfer between CIS@ZnS-TSA and oxidant can give off obvious flash CL under optimum conditions. The maximum emission wavelength for CL of CIS@ZnS-TSA is adjustable from 730 nm to 823 nm via employing different triggering agents. Promisingly, the coexistent reductant of N2H4·H2O and oxidant of H2O2 can be employed as dual triggering reagents to trigger eye-visible and highly efficient flash CL from CIS@ZnS-TSA. The maximum emission intensity for flash CL of CIS@ZnS-TSA/N2H4–H2O2 is 101-fold greater than the glow CL of CIS@ZnS-TSA/N2H4 and 22-fold greater than the flash CL of CIS@ZnS-TSA/H2O2, respectively. The flash CL from CIS@ZnS-TSA/N2H4–H2O2 is qualified for highly sensitive and selective CL immunoassay in a commercialized typical procedure with the entire operating process manually terminated within 35 min.
研究领域:
纳米技术
·
分析化学
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2021
·
IF 4.464
Xia Qiu,Ling Wang,Yinggui Zhu
A novel electrochemiluminescence (ECL) system consisting of Eosin B (EB) and K 2 S 2 O 8 was studied and found to generate ECL following the oxidation or reduction of Eosin B and K 2 S 2 O 8 . The type of buffer solution, type of co-reactant, pH value, and K 2 S 2 O 8 concentration were optimized through experiments to make the reaction system reach the best value. In addition, we found that Cr 3+ has a great quenching effect on the EB reaction system. Through the comparison of ECL and CV under different conditions, DPV curve, UV spectrum and ECL spectrum experiments, we focused on the quenching mechanism of Cr 3+ on the system and made reasonable guesses. This method demonstrates the potential application value of ECL in the field of environmental monitoring and reveals the potential value of organic molecular ECL luminophores.
研究领域:
分析化学
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环境科学
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电化学
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产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2023
·
IF 7.4
Xiaoyue Zhang,Xuan Kuang,Xiang Ren,Yuewei Wang,Xuejing Liu,Yueyun Li,Huangxian Ju,Qin Wei
Copper nanoclusters (Cu NCs) are highly promising nanomaterials in the field of electrochemiluminescence (ECL). Nevertheless, their limited stability and efficiency have impeded their practical applications. Here, we introduced a novel supramolecular anchoring strategy resulting in the creation of exceptionally stable Cu NCs (CET-Cu NCs) with remarkable ECL properties. Specifically, CET-Cu NCs exhibited a relative ECL efficiency (ΦECL) of 62% based on the annihilation ECL efficiency of [Ru(bpy)3]2+ (100%), with tripropylamine employed as a coreactant. Moreover, CET-Cu NCs can generate ECL emission through multiple different paths, which enables them to serve as signal probes in a wider range of testing scenarios, thereby enhancing the reliability and robustness of sensing and analytical systems. To demonstrate the practical utility, CET-Cu NCs were selected as an ECL signal probe for a sensing platform that facilitated ultrasensitive detection of progesterone via oriented immobilization technology and antibody/aptamer sandwich assays. This study surmounted the barriers to the practical application of Cu NCs through the implementation of a supramolecular anchoring strategy, thereby providing enhanced utility of Cu NCs in ECL sensing and analysis.
研究领域:
分析化学
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生物传感
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纳米材料科学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Li Fu,Pengjie Dong,Zerui Liu,Qi Li,Yingshu Guo
Screening a novel electrochemiluminescence (ECL) system and lowering the ECL triggering potential are essential to ECL evolution. Herein, the near-infrared (NIR) ECL system with low-triggering potential ECL was constructed with weakly reductive tert-butylamine borane as coreactant and mercaptosuccinic acid/citrate (MSA/Cit)-capped Au (MSA/Cit@AuNCs) as luminophores. Toxic-element-free and dual-ligand MSA/Cit@AuNCs were prepared via ligand exchange and utilized as a model for developing unary metal NCs-based luminophores with more enhanced ECL performance than monoligand Au nanocrystals (AuNCs), which exhibited a two hole-injected process at around 0.48 and 0.80 V, respectively. Beneficial to the intrinsic low hole-injected potential of AuNCs, MSA/Cit@AuNCs exhibited similar low-triggering ECL potential at around 0.57 V with the participation of series coreactants or not, originating from the recombination of an internal prestored electron within the conduction band (CB) and electroinjected holes at around 0.25 V. Furthermore, the enhanced low-triggering potential around 0.57 V and NIR ECL around 835 nm of MSA/Cit@AuNCs was eventually obtained with the reductive tert-butylamine borane or N2H4·H2O containing a −C–N single-bond structure merely as coreactant. The low-triggering potential ECL of MSA/Cit@AuNCs/tert-butylamine borane system at 0.57 V can be harnessed to selectively determine a carcinoembryonic antigen (CEA) with one linear range spanning from 2 to 20000 fg/mL and a limit of detection of 0.33 fg/mL (S/N = 3). This study will contribute to a more comprehensive understanding of the ECL mechanism in terms of both regulating NCs and selecting coreactants.
研究领域:
分析化学
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纳米技术
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生物化学
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF LUMINESCENCE
·
2024
·
IF 3.3
Yiran Ying,Yuting Li,Jing Wu
Exploring new materials to enhance chemiluminescence (CL) and constructing novel CL systems are essential for expanding the application range of CL analysis and improving its sensitivity. Metal-organic frameworks (MOFs) have gained popularity in catalysis and other fields due to their unique structural features and properties. However, the research on the application of MOFs in CL is still in its infancy. In this study, one kind of mixed-ligand metal-organic frameworks using Zn as the metal ion (Zn m-MOFs) was successfully synthesized and applied to enhance the CL of NaClO-H 2 O 2 system. The synthesized Zn m-MOFs catalyzed the generation of reactive oxygen species (ROS) (·O 2 − , 1 O 2 and ·OH) which injected holes and electrons to Zn m-MOFs to generate hole-injected and electron-injected Zn m-MOFs (Zn m-MOFs ⋅+ and Zn m-MOFs ⋅− ). The subsequent annihilation of these charge carriers led to enhanced CL. At the same time, chemiluminescence resonance energy transfer (CRET) occurred during the process. Compared with the NaClO-H 2 O 2 system without Zn m-MOFs, the CL intensity of the Zn m-MOFs-NaClO-H 2 O 2 system was enhanced by approximately 1000 times. This study aims to provide new insights for the development of innovative CL systems based on MOFs, thereby broadening the application fields of CL analysis.
研究领域:
分析化学
·
材料科学
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产品关联
DOI
高影响因子
TALANTA
·
2025
·
IF 6.1
Shanshan Wu,Xinyu Li,Bijun Wang,Jingshuo Jiang,Di Tao,Zhenhong Xia,Hang Gao,Ningning Wang
The development of convenient and highly sensitive methodologies for evaluating drug levels in serum is crucial for clinical diagnosis. As the most prescribed drug against hyperthyroidism, methimazole (MMZ) lacks the simple and rapid quantitative method. Herein, an electrochemiluminescent (ECL) sensor is proposed for MMZ detection using an aggregation-induced delayed ECL (AIDECL) active organic nanodots (ODs) as a probe. Due to the full utilization of excited species, these ODs exhibit high ECL efficiency. In the presence of MMZ, such sensor exhibits decreased ECL signal of ODs since MMZ can consume co-reactants’ radical cations that are vital mediates for ECL emission of ODs. Consequently, the developed sensor demonstrates excellent performance for the analysis of MMZ with a low detection limit of 7 × 10 −7 μM, good specificity, and excellent stability. Significantly, this sensor has been successfully applied to serum sample with good recovery rate. This work provides a valuable strategy for accurate detection of MMZ, and demonstrates its application potential in clinical research.
研究领域:
分析化学
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临床诊断
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药物检测
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产品关联
DOI
PubMed
高影响因子
Foods
·
2025
·Vol. 14
·期 3
·538
·
IF 5.1
Jie Wu,Yuanjie Qin,Xiaoxin Mei,Lin Cai,Wen Hao,Guozhen Fang
Ciprofloxacin (CIP), a widely used broad-spectrum antibiotic, poses a serious threat to human health and environmental safety due to its residues. The complementary monomers molecularly imprinted electrochemiluminescence sensor (MIECLS) based on a polyvinylpyrrolidone-functionalized copper nanowires (CuNWs@PVP) luminescent probe was constructed for the ultra-sensitive detection of CIP. CuNWs with low cost and high conductivity exhibited near-infrared electrochemiluminescence (NIR ECL) properties, yet their self-aggregation and oxidation led to a weakened emission phenomenon. PVP with solvent affinity and large skeleton was in situ attached to CuNWs surface to avoid CuNWs sedimentation and aggregation, and self-enhanced ECL signals were achieved. The bifunctional monomers molecularly imprinted polymer (MIP) possessed complementary active centers that increased their affinity with CIP, enhancing the accurate and sensitive detection of the target substances. The linear range of CIP using MIECLS was 5.00 × 10 −9 –5.00 × 10 −5 mol L −1 with a low limit of detection (LOD) of 2.59 × 10 −9 mol L −1 , while the recovery rates of CIP in the spiking recovery experiment were 84.39% to 92.48%. The combination of bifunctional monomer MIP and NIR copper-based nano-luminescent probe provides a new method for the detection of CIP in food.
研究领域:
分析化学
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食品安全
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生物传感技术
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2019
·
IF 9.518
Xuwen Gao,Kena Fu,Li Fu,Huaisheng Wang,Bin Zhang,Guizheng Zou
Novel optical labels for biosensing in near-infrared (NIR) region (especially between 800 and 900 nm) are arousing much attention for higher penetrating capability, less scattering and lowered autofluorescent background. Herein, a water-soluble electrochemiluminophore with effective electrochemiluminescence (ECL) around 815 nm is developed via doping dual-stabilizers-capped CdTe nanocrystals (NCs) with Co 2+ species in a growth-doping way. The Co 2+ -doped CdTe NCs not only can preserve the highly-passivated surface states of dual-stabilizers-capped CdTe NCs, but also exhibit efficient red-shifted photoluminescence (PL) and ECL into the promising optical NIR window of 800–900 nm. A spectrum-based ultrasensitive NIR ECL immunosensor is consequently fabricated with the Co 2+ -doped CdTe NCs as tags for the first time, which can selectively and sensitively determine human carcinoembryonic antigen with a wide linearity range from 1 fg/mL to 10 pg/mL and a low limit of detection at 0.2 fg/mL (S/N = 3). This work opens a way to screen novel NIR electrochemiluminophore as well as to modulate the ECL performance of NCs via surface doping and engineering.
研究领域:
材料科学
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纳米技术
·
分析化学
·
生物传感器
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2014
·
IF 3.84
Zi-Ming Zhou,Zhe Feng,Jun Zhou,Bi-Yun Fang,Zhi-Ya Ma,Bo Liu,Yuan-Di Zhao,Xue-Bin Hu
A new method is proposed for determination of carcino-embryonic antigen (CEA) based on quantum dot (QD)-modified aptamer probe by capillary electrophoresis-chemiluminescence (CE-CL) detection system. An oligonucleotide sequence A contains two parts, one is CEA aptamer and the other is bridging segment. Horseradish peroxidase (HRP) conjugates with sequence A (HRP-DNA A ) and then mixes with QD labeling complementary bridging segment (QD-DNA B ), forming HRP-DNA A-B -QD probe. When CEA exists, the specific combination of the aptamer in sequence A and CEA can form CEA/HRP-DNA A-B -QD complex. After separation of CEA/HRP-DNA A-B -QD complex and HRP-DNA A-B -QD probe by capillary electrophoresis (CE), the chemiluminescence (CL) catalyzed by CEA/HRP-DNA A-B -QD complex can be detected without any interference, and the content of CEA can be estimated by the CL intensity. It has been proved that the separation efficiency of HRP-DNA A-B -QD and CEA/HRP-DNA A-B -QD complex is improved greatly after QD modification, then the interference issue resulted from free HRP-DNA A-B -QD probe is solved well; and the specific combination of HRP-DNA A-B -QD and CEA leads to a closer distance of HRP and QD, then chemiluminescent resonance energy transfer (CRET) occurs, which confirms the validity of the strategy. Results show that the CL intensity has a linear relationship with the concentration of CEA in the range from 0.0654 to 6.54 ng/mL, and the limit of detection is approximately 8 pg/mL ( S / N = 3). This proposed method with high specificity has been applied for the accurate analysis of content of CEA in patient's serum.
研究领域:
分析化学
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生物技术
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纳米技术
·
医学诊断
✓ 已验证
产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2023
·
IF 4.4
Tong Shao,Xiaolei Song,Yufeng Jiang,Chenchen Wang,Peng Li,Shihao Sun,Dingzhong Wang,Wei Wei
Among various chemiluminescence (CL) systems, luminol-H 2 O 2 system is used extensively due to its cheapness and sensitivity. Herein, 4-hydroxy-3-methoxybenzaldehyde, known as vanillin, was firstly found to be able to catalyze H 2 O 2 very efficiently to produce • OH and O 2 •− , which can be used to enhance the CL of luminol-H 2 O 2 as Co + . In alkaline aqueous solution, vanillin catalyzed the dissociation of H 2 O 2 into active • OH and O 2 •− radicals and accelerated luminol-H 2 O 2 reaction to emit strong CL signal. Combining the stabilizing function of β-CD, CL intensity of luminol-H 2 O 2 was enhanced further. Thus, dual-signal amplification of luminol-H 2 O 2 chemiluminescence based on the catalyzing function of vanillin and the stabilizing function of β-CD was proposed and its mechanism was explored deeply in the manuscript. Interestingly, vanillin is a highly prized flavor compound broadly used as food additive, however, the excessive intake of vanillin is harmful to human and thus the determination of vanillin is very important. On the basis of the luminol-β-CD-H 2 O 2 /vanillin reaction, a low-cost, rapid and simple CL sensor has been established to detect vanillin. The sensor was able to detect vanillin in the range of 1.0 μM ∼ 75 μM with a detection limit of 0.89 μM (S/N = 3). It can also be used for CL imaging detection with satisfactory results.
研究领域:
分析化学
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食品科学
·
化学传感器
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LUMINESCENCE
·
2025
·Vol. 40
·期 4
·e70175
·
IF 3
Jiaoyan Xie,Suqin Han
Folic acid (FA) is a crucial B vitamin for cell formation and maintenance, and its deficiency can lead to severe health disorders, highlighting the importance for accurate FA detection. Chemiluminescence (CL) provides a highly sensitive analytical approach, characterized by simple instrumentation, high accuracy, and low background interference. However, traditional CL techniques are often limited by weak signal intensity and poor robustness. This study presents a novel CL system using well-defined fluorescent Cl, S, N co-doped carbon dots (ClSN-CDs) integrated into the H 2 O 2 -KBrO 3 system. Under acidic conditions, the synergistic effect of H 2 O 2 and KBrO 3 induced ClSN-CDs to emit a strong CL signal, with the emitting species identified as excited-state ClSN-CDs. Trace amounts of FA significantly quenched the CL intensity of the ClSN-CDs-H 2 O 2 -KBrO 3 system, enabling rapid, sensitive, and selective FA detection. The system demonstrated a linear response to FA concentrations ranging from 0.02 to 1.0 μM, with a detection limit of 3.1 nM. Selectivity and recovery studies validated the system's robust analytical performance. Mechanistic investigations revealed that the CL reaction involved reactive oxygen species (ROS), and FA-mediated ROS consumption led to luminescence suppression.
研究领域:
分析化学
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营养学
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食品科学
✓ 已验证
DOI
PubMed
高影响因子
MOLECULES
·
2022
·Vol. 27
·期 20
·6863
·
IF 4.927
Bolin Zhao,Xingzi Zou,Jiahui Liang,Yelin Luo,Xianxi Liang,Yuwei Zhang,Li Niu
Developing excellent strategies to optimize the electrochemiluminescence (ECL) performance of C3N4materials remains a challenge due to the electrode passivation, causing weak and unstable light emission. A strategy of controlling the calcination atmosphere was proposed to improve the ECL performance of C3N4nanotubes. Interestingly, we found that calcination atmosphere played a key role in specific surface area, pore-size and crystallinity of C3N4nanotubes. The C3N4nanotubes prepared in the Air atmosphere (C3N4NT-Air) possess a larger specific surface area, smaller pore-size and better crystallinity, which is crucial to improve ECL properties. Therefore, more C3N4•−excitons could be produced on C3N4NT-Air, reacting with the SO4•−during the electrochemical reaction, which can greatly increase the ECL signal. Furthermore, when C3N4nanotube/K2S2O8system is proposed as a sensing platform, it offers a high sensitivity, and good selectivity for the detection of Cu2+, with a wide linear range of 0.25 nM~1000 nM and a low detection limit of 0.08 nM.Keywords:electrochemiluminescence;C3N4nanotube;Cu2+detection
研究领域:
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）