Biology-Basel
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2025
·Vol. 14
·期 12
·1746
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IF 3.5
Shuhui Fu,Fangyuan Li,Xizhong Yan,Chi Hao
Phytophagous insects rely on highly evolved chemosensory systems to recognize plant cues, which guide essential behaviors such as feeding and oviposition. While the antennal chemosensory functions of Plutella xylostella are well-studied, little is known about the role of its legs in detecting host plant chemicals. This study systematically identified and characterized chemosensory-related genes expressed in the legs of P. xylostella using transcriptome data. A total of 114 leg-expressed chemosensory genes were identified, with PxylCSP9 showing high expression in legs and strong, broad-spectrum binding to key cruciferous plant volatiles. Leg chemosensory proteins, particularly PxylCSP9, play an important role in the chemical perception. Plutella xylostella , a major pest of cruciferous vegetables, depends predominantly on chemoreception to locate host plants. Legs are crucial in insect chemical perception, particularly during close-range and contact chemoreception. However, the molecular basis underlying the chemosensory repertoire in P. xylostella legs remains elusive. To address this, we sequenced chemosensory-related genes in diamondback moth legs. Sequencing identified 32 odorant binding protein (OBP), 18 chemosensory protein (CSP), 26 odorant receptor (OR), 20 gustatory receptor (GR), 15 ionotropic receptor (IR), and 3 sensory neuron membrane protein (SNMP) genes. Comparative analysis with antennal transcriptome data revealed three CSPs, seven ORs, and two GRs newly identified in the legs. Transcriptome analysis showed higher fragments per kilobase of transcript per million mapped reads values for CSPs than for other chemosensory-related gene families. Furthermore, qRT-PCR confirmed the highest expression of PxylCSP9 in the legs, suggesting its role in perceiving external compounds. Fluorescent binding assays revealed high binding affinity of PxylCSP9 for several host plant semiochemicals. Molecular docking predicted a hydrophobic binding pocket in PxylCSP9 with Met11, Leu13, and Leu43 frequently participating in ligand interactions. Our findings indicate that leg-enriched PxylCSP9 is pivotal for host plant recognition during close-range chemoreception, suggesting its potential as a molecular target for precision management through behavior-based strategies.
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PHYSICAL BIOLOGY
·
2022
·Vol. 19
·期 4
·046004
·
IF 2.959
Yue Yang,Caijuan Yue,Chi Zhang,Junhua Yuan
Polymers are important components of the complex fluid environment for microorganisms. The mechanical effects on bacterial motile behavior due to the viscous or viscoelastic properties of polymers were extensively studied, whereas possible chemical effects on bacterial motility through bacterial chemoreception of the polymers were unclear. Here we studied the chemotactic response of Escherichia coli to polymeric solutions by combining the bead assay and FRET measurements. We found that the wild-type E. coli strain exhibited an attractant response to widely used polymers such as Ficoll 400, polyethylene glycol (PEG) 20000 and polyvinyl pyrrolidone (PVP) 360000, and the response amplitude from chemoreception was much larger than that from the load-dependence of motor switching due to viscosity change. The chemotactic response depended on the type of receptors and the chain length of the polymers. Our findings here provided important new ingredients for further studies of bacterial motile behavior in complex fluids.
研究领域:
微生物学
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生物物理学
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BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
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2024
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IF 4.2
Jia-Qi Huang,Li Zhang,Fan Wu,Jing Tan,Ping Wen,Wei Xu,Hong-Liang Li
As a native bee species, the eastern honeybee ( Apis cerana ) plays an essential role in pollinating loquat flowers, which bloom in early winter in China. This pollination behavior is closely related to A. cerana 's ability to adapt to low temperatures, which depends on the functionality of its chemoreceptive system. Transcriptome analysis revealed a significant upregulation of the A. cerana chemosensory protein 1 (CSP1) gene at low temperatures. Fluorescence competitive binding experiments indicated that nine chemical volatiles from loquat flowers exhibited a stronger binding affinity to CSP1 than to odorant binding protein 2 (OBP2). Thermodynamic analysis revealed that CSP1's binding affinity increases at low temperatures, with a static binding mechanism largely influenced by the specific volatile molecule rather than the type of olfactory soluble protein. Molecular docking and site-directed mutagenesis confirmed that F44 residue may play a key role in CSP1's binding to three primary volatile compounds. In summary, the present study identified a dual sensing mechanism in which low temperatures upregulated the expression of CSP1 and enhanced the binding affinity of CSP1 to loquat flower volatiles. These findings not only clarify A. cerana 's chemoreceptive mechanism toward loquat flower volatiles in pollination but also provide a theoretical basis for further exploring ecological adaptations between native bees and early-winter flowering plants.
研究领域:
昆虫学
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分子生物学
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生态学
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高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2025
·
IF 6.2
Ya-Lan Sun,Ya-Hui Sun,Ke Wang,Pengjun Xu,Jun-Feng Dong
Helicoverpa armigera is one of the most serious pests attacking tobacco around the world. Nicotiana rustica is widely used as a trap crop in the fields of Nicotiana tabacum in China. However, the chemical and molecular bases guiding N. rustica recognition in H. armigera are still unknown. In this study, we found 15 volatiles emitted from N. rustica induced EAG responses of H. armigera, while β-ocimene, α-pinene, and linalool were attractive to the moths in Y-tubes. Oviposition tests showed that H. armigera prefers to lay eggs on tobacco plants treated with β-ocimene and α-pinene. Further assays showed that an antenna-enriched chemosensory protein, HarmCSP1, binds robustly with β-ocimene, benzaldehyde, and (Z)-3-hexenyl acetate. Structural analyses revealed that several key amino acid residues are involved in ligand binding. This study increases the understanding of the chemosensory mechanism of H. armigera’s recognition of N. rustica and provides the foundation for the design of attractants for H. armigera management.
研究领域:
昆虫学
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分子生物学
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化学生态学
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高影响因子
Frontiers in Physiology
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2025
·
IF 3.4
Long Liu,Yangdi Li,Hua Yang,Fan Wang,Qiong Huang
Odorant-binding proteins (OBPs) are important for insects to discriminate, bind and transport odorants, such as pheromones and host plant volatiles. Herein, the Minus-C OBP (CbuqOBP1) was characterized from Cyrtotrachelus buqueti , one of the most important pests in bamboo plantations. CbuqOBP1 showed significantly higher transcription levels in the adult stage and was most highly expressed in the head of both sexes, the thorax and antenna of the male, indicating that it plays important roles in chemosensory behavior of adults and may also function in other biological processes. Fluorescence competitive binding assays showed that CbuqOBP1 displayed broad binding capabilities and strong affinities to phenol ( K i = 10.49 μM) and benzothiazole ( K i = 11.11 μM) among 8 C. buqueti volatiles. CbuqOBP1 also showed high binding affinity to the main volatile of the host plant Neosinocalamus affinis (linalool, K i = 13.41 μM). The docking results indicated that hydrophobic interactions were the prevailing forces between CbuqOBP1 with these three ligands. Additionally, several amino acid residues were significantly overlapped and contributed to the interactions with the ligands. The combined results suggest that CbuqOBP1 may play dual roles in binding volatile compounds from the host plant and the same species and will be helpful to developing new pest-control strategies.
研究领域:
昆虫学
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分子生物学
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JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
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2024
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IF 11.4
Haiman Xu,Lianxia Guo,Tingying Hao,Xiaocao Guo,Meiping Huang,Haobin Cen,Min Chen,Jiaxian Weng,Meixia Huang,Zicong Wu,Zifei Qin,Jing Yang,Baojian Wu
Background While the daily rhythm of allergic rhinitis (AR) has long been recognized, the molecular mechanism underlying this phenomenon remains enigmatic. Objective We aim to investigate the role of circadian clock in AR development and to clarify the mechanism by which the daily rhythm of AR is generated. Methods AR was induced in mice using the ovalbumin method. Toluidine blue staining, LC-MS/MS analysis, qPCR, and immunoblotting were performed with AR and control mice. Results Ovalbumin-induced AR is diurnally rhythmic and associated with clock gene disruption in nasal mucosa. In particular, Rev-erbα is generally down-regulated, and its rhythm retained but with a near 12-h phase shift. Furthermore, global knockout of the core clock gene Bmal1 or Rev-erbα increases the susceptibility of mice to AR and blunts AR rhythmicity. Importantly, nasal SCCs (solitary chemosensory cells) are rhythmically activated, and inhibition of the SCC pathway leads to attenuated AR and a loss of its rhythm. Moreover, rhythmic activation of SCCs is accounted for by diurnal expression of ChAT (an enzyme responsible for the synthesis of acetylcholine) and temporal generation of the neurotransmitter acetylcholine. Mechanistically, REV-ERBα trans-represses Chat through direct binding to a specific response element, generating a diurnal oscillation in this target gene. Conclusion These findings identify SCCs, under the control of REV-ERBα, as a driver of AR rhythmicity, and suggest targeting SCCs as a new avenue for AR management.
研究领域:
免疫学
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时间生物学
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分子生物学
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2022
·
IF 8.025
Zhao Liu,Qian-Ping Xie,Huai-Wang Guo,Wei Xu,Jin-Jun Wang
Odorant-binding proteins (OBPs) are believed to play critical roles in host-seeking behavior. However, little attention was paid to its different functions in male and female. The antenna-specific OBP gene from Bactrocera dorsalis , BdorOBP13, was cloned and its expression profile was examined. The results showed that BdorOBP13 was exclusively expressed in male and female adults, which exhibited a high transcript level in antennae. After injection of BdorOBP13 dsRNA, its transcript level in males and females decreased significantly. Electrophysiological responses of RNAi-injected flies to, methyl eugenol (male attractant) and γ-octalactone (female attractant) decreased significantly. However, no significant changes in the electrophysiological response were observed in RNAi-injected flies to benzothiazole, (+),dipentene, and ethyl tiglate. The behavioral bioassay showed that males treated with RNAi significantly reduced their preference to methyl eugenol, while RNAi-injected females showed a significantly lower preference to γ-octalactone, suggesting that BdorOBP13 may have different functions between males and females: it may be involved in the detection of methyl eugenol in males but is involved in the detection of γ-octalactone in females. These findings improve our understanding of insect OBPs and their roles in insect chemosensation, which may provide us with new molecular targets in the management of B. dorsalis .
研究领域:
昆虫学
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分子生物学
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害虫管理
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JOURNAL OF INVERTEBRATE PATHOLOGY
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2024
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IF 3.6
Peng-hua Bai,Jin-Ping Yu,Rui-rui Hu,Qian-wen Fu,Hai-chao Wu,Xing-yue Li,Guo-hao Zu,Bao-sheng Liu,Yu Zhang
Entomopathogenic nematodes (EPNs) use the chemical cues emitted by insects and insect-damaged plants to locate their hosts. Steinernema carpocapsae , a species of EPN, is an established biocontrol agent used against insect pests. Despite its promising potential, the molecular mechanisms underlying its ability to detect plant volatiles remain poorly understood. In this study, we investigated the response of S. carpocapsae infective juveniles (IJs) to 8 different plant volatiles. Among these, carvone was found to be the most attractive volatile compound. To understand the molecular basis of the response of IJs to carvone, we used RNA-Seq technology to identify gene expression changes in response to carvone treatment. Transcriptome analysis revealed 721 differentially expressed genes (DEGs) between carvone-treated and control groups, with 403 genes being significantly upregulated and 318 genes downregulated. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that the responsive DEGs to carvone attraction were mainly involved in locomotion, localization, behavior, response to stimulus, and olfactory transduction. We also identified four upregulated genes of chemoreceptor and response to stimulus that were involved in the response of IJs to carvone attraction. Our results provide insights into the potential transcriptional mechanisms underlying the response of S. carpocapsae to carvone, which can be utilized to develop environmentally friendly strategies for attracting EPNs.
研究领域:
昆虫学
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分子生物学
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植物科学
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生物防治
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高影响因子
PEST MANAGEMENT SCIENCE
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2024
·
IF 3.8
Shan-Cheng Yi,Xin-Hui Chen,Yu-Hang Wu,Juan Wu,Jia-Qing Wang,Man-Qun Wang
BACKGROUND The important wood-boring pest Batocera horsfieldi has evolved a sensitive olfactory system to locate host plants. Odorant-binding proteins (OBPs) are thought to play key roles in olfactory recognition. Therefore, exploring the physiological function of OBPs could facilitate a better understanding of insect chemical communications. RESULTS In this research, 36 BhorOBPs genes were identified via transcriptome sequencing of adults' antennae from B. horsfieldi , and most BhorOBPs were predominantly expressed in chemosensory body parts. Through fluorescence competitive binding and fluorescence quenching assays, the antenna-specific BhorOBP28 was investigated and displayed strong binding affinities forming stable complexes with five volatiles, including (+)-α-Pinene, (+)-Limonene, β-Pinene, (−)-Limonene, and (+)-Longifolene, which could also elicit conformation changes when they were interacting with BhorOBP28. Batocera horsfieldi females exhibited a preference for (−)-Limonene, and a repellent response to (+)-Longifolene. Feeding dsOBP19 produced by a bacteria-expressed system with a newly constructed vector could lead to the knockdown of BhorOBP28 , and could further impair B. horsfieldi attraction to (−)-Limonene and repellent activity of (+)-Longifolene. The analysis of site-directed mutagenesis revealed that Leu7, Leu72, and Phe121 play a vital role in selectively binding properties of BhorOBP28. CONCLUSION By modeling the molecular mechanism of olfactory recognition, these results demonstrate that BhorOBP28 is involved in the chemoreception of B. horsfieldi . The bacterial-expressed dsRNA delivery system gains new insights into potential population management strategies. Through the olfactory process concluded that discovering novel behavioral regulation and environmentally friendly control options for B. horsfieldi in the future. © 2024 Society of Chemical Industry.
研究领域:
昆虫学
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分子生物学
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化学生态学
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昆虫生理学
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PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
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2023
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IF 4.7
Ning-Na Yin,Yu-Juan Yao,Yin-Lan Liang,Zheng-Quan Wang,Yong-He Li,Nai-Yong Liu
The orientation of the oligophagous cone-feeding moth Dioryctria abietella (Lepidoptera: Pyralidae) to host plants primarily relies on olfactory-related proteins, particularly those candidates highly expressed in antennae. Here, through a combination of expression profile, ligand-binding assay, molecular docking and site-directed mutagenesis strategies, we characterized the chemosensory protein (CSP) gene family in D. abietella . Quantitative real-time PCR (qPCR) analyses revealed the detectable expression of all 22 DabiCSPs in the antennae, of which seven genes were significantly enriched in this tissue. In addition, the majority of the genes (19/22 relatives) had the expression in at least one reproductive tissue. In the interactions of four antenna-dominant DabiCSPs and different chemical classes, DabiCSP1 was broadly tuned to 27 plant-derived odors, three man-made insecticides and one herbicide with high affinities (K i < 6.60 μM). By contrast, three other DabiCSPs (DabiCSP4, CSP6 and CSP17) exhibited a narrow odor binding spectrum, in response to six compounds for each protein. Our mutation analyses combined with molecular docking simulations and binding assays further identified four key residues (Tyr25, Thr26, Ile65 and Val69) in the interactions of DabiCSP1 and ligands, of which binding abilities of this protein to 12, 15, 16 and three compounds were significantly decreased compared to the wildtype protein, respectively. Our study reveals different odor binding spectra of four DabiCSPs enriched in antennae and identifies key residues responsible for the binding of DabiCSP1 and potentially active compounds for the control of this pest.
研究领域:
昆虫学
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分子生物学
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化学生态学
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PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
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2018
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IF 3.44
Xinda Lin,Yiwen Mao,Ling Zhang
Insects receive a variety of chemical signals from the environment. Chemosensory protein (CSP) is one of the olfactory proteins that can accommodate a variety of small molecules and have the ability to bind to lipophilic compounds, transmitting nonvolatile odor molecules and chemical stimuli to target cells. To understand the correlation between the insect olfactory system and environment, we identified four antennae-expressed SlituCSP genes and investigated their expression profiles after treatment with different temperatures, starvation and three commonly used pesticides : chlorpyrifos , emamectin benzoate and fipronil . The transcriptions of four SlituCSP genes are affected by pesticide treatment and less affected by starvation and different temperatures. To further understand the molecular function of CSPs and their correlation with pesticides, we expressed and purified four SlituCSPs and assayed their binding ability with pesticides. The binding of four SlituCSPs with three pesticides were determined using a fluorescence competitive binding assay. We found direct binding between CSPs and pesticides, especially between SlituCSP18 and chlorpyrifos/fipronil and between SlituCSP6 and all three pesticides. The high binding affinity with pesticides and the significant down-regulation of SlituCSP18 by chlorpyrifos suggests that SlituCSP18 is more sensitive to pesticide treatment and may play an important role in mediating the interaction of the olfactory system and the pesticide. This study can help us understand the role of CSP proteins in the adaption of S. litura to the environment.
研究领域:
昆虫学
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分子生物学
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环境科学
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高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2024
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IF 5.7
Jingyu Zhao,Huanhuan Sun,Gege Wang,Qi Wang,Yipeng Wang,Qingbin Li,Shuangyu Bi,Qingsheng Qi,Qian Wang
Two-component systems (TCSs) sensing and responding to various stimuli outside and inside cells are valuable resources for developing biosensors with synthetic biology applications. However, the use of TCS-based biosensors suffers from a limited effector spectrum, hypersensitivity, low dynamic range, and unwanted signal crosstalk. Here, we developed a tailor-made Escherichia coli whole-cell γ-aminobutyric acid (GABA) biosensor by engineering a chimeric GABA chemoreceptor PctC and TCS. By testing different TCSs, the chimeric PctC/PhoQ showed the response to GABA. Chimera-directed evolution and introduction of the insulated chimeric pair PctC/PhoQ*PhoP* produced biosensors with up to 3.50-fold dynamic range and good orthogonality. To further enhance the dynamic range and lower the basal leakage, three strategies, engineering of PhoP DNA binding sites, fine-tuning reporter expression by optimizing transcription/translation components, and a tobacco etch virus protease-controlled protein degradation, were integrated. This chimeric biosensor displayed a low basal leakage, a large dynamic range (15.8-fold), and a high threshold level (22.7 g L–1). Finally, the optimized biosensor was successfully applied in the high-throughput microdroplet screening of GABA-overproducing Corynebacterium glutamicum, demonstrating its desired properties for extracellular signal biosensing.
研究领域:
合成生物学
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生物技术
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分子生物学
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微生物学
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高影响因子
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