CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
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2016
·Vol. 32
·期 6
·943-946
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IF 1.086
Li Min,Jin Yongri,Wang Xiaozhong,Wu Qian,Liu Ying,Li Peng,Li Xuwen
A new triterpenoid glycoside( 1 ) and seven known triterpenoid glycosides, pseudoginsenoside RT 2 ( 2 ), yesanchinoside R 2 ( 3 ), vinaginsenoside R13( 4 ), vinaginsenoside R8( 5 ), notoginsenoside E( 6 ), 6′′′- O -acetylginsenoside Re( 7 ), 6″- O -acetylginsenoside Rb 1 ( 8 ), were isolated from the rhizomes of Panacis majoris . The new triterpenoid glycoside was elucidated as 3- O -[ β - D -glucopyranosyl-(1→2)- β - D -(6′- O -ethyl)-glucuronopyranosyl]-oleanolic acid-28- O - β - D -glucopyranoside by extensive spectroscopic and phytochemical methods. Compounds 2 ― 8 were obtained from the plant for the first time. Compounds 3 and 4 displayed good activities against adenosine diphosphate (ADP)-induced platelet aggregation, and compounds 1, 5, 6 and 8 showed moderate activities. Compound 6 exhibited moderate antiplatelet aggregation activity induced by arachidonic acid(AA).
研究领域:
天然产物化学
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药用植物学
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心血管药理学
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药物代谢
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分子药理学
✓ 已验证
DOI
高影响因子
Biomed Research International
·
2020
·Vol. 2020
·期 1
·5084057
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IF 2.276
Li Yang,Yiwei Fang,Ronghua Liu,Junwei He
Dendropanax dentiger root is a traditional medicinal plant in China and used to treat inflammatory diseases for centuries, but its phytochemical profiling and biological functions are still unknown. Thus, a rapid, efficient, and precise method based on ultra high-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UHPLC-Q-TOF-MS/MS) was applied to rapidly analyse the phytochemical profiling of D. dentiger with anti-inflammatory and antioxidant activities in vitro . As a result, a total of 78 chemical compositions, including 15 phenylpropanoids, 15 alkaloids, 14 flavonoids, 14 fatty acids, 7 phenols, 4 steroids, 4 cyclic peptides, 3 terpenoids, and 2 others, were identified or tentatively characterized in the roots of D . dentiger . Moreover, alkaloid and cyclic peptide were reported from D . dentiger for the first time. In addition, the ethanol crude extract of D. dentiger roots exhibited remarkable anti-inflammatory activity against cyclooxygenase- (COX-) 2 inhibitory and antioxidant activities in vitro . This study is the first to explore the phytochemical analysis and COX-2 inhibitory activity of D. dentiger . This study can provide important phytochemical profiles and biological functions for the application of D. dentiger roots as a new source of natural COX-2 inhibitors and antioxidants in pharmaceutical industry.
研究领域:
天然药物化学
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药用植物学
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炎症生物学
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自由基生物学
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药物代谢
✓ 已验证
DOI
高影响因子
Current Analytical Chemistry
·
2024
·Vol. 21
·期 7
·884-897
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IF 1.7
Tian-Hao Pei,Yu-Long Niu,Bin Liu,Ming Yuan,Ai-Guo Zhang,Xiao-Feng Li,Lian-Xia Wang,Meng-Lei Xu,Yu Gao
BackgroundEssential oils (EOs) are recognized for their potent insecticidal activity and are widely considered promising agents for pest control.MethodsThe insecticidal activity of seven commercial citrus EOs against the global phytophagous pest Thrips flavus was evaluated using bioassays conducted under laboratory conditions. Moreover, the chemical composition of these EOs was analyzed using gas chromatography-mass spectrometry (GC-MS).ResultsAmong the 45 identified compounds, the major constituents were d-limonene (23.77%–95.10%), methyl jasmonate (38.76%), and linalyl acetate (34.55%). Orange flower oil, sweet orange oil, tangerine peel oil, bergamot oil, lime oil, lemon oil, and grapefruit oil exhibited good insecticidal activity against T. flavus without demonstrating significant repellent activity. Orange flower oil and sweet orange oil showed higher insecticidal toxicity, with LC50 values of 0.20 g/L and 0.37 g/L, respectively. In pot experiments, the higher control efficacy against T. flavus was found after 7 days of treatment for orange flower oil (91.14±1.27% at 720.00 g a.i.·hm−2 and 100% at 900.00 g a.i.·hm−2), sweet orange oil (91.14±5.52% at 900.00 g a.i.·hm−2), tangerine peel oil (96.20±2.19% at 720 g a.i.·hm−2 and 100% at 900.00 g a.i.·hm−2), lemon oil (96.20±2.19% at 900 g a.i.·hm−2), and lime oil (97.47±2.53% at 900.00 g a.i.·hm−2).ConclusionTaken together, orange flower oil and tangerine peel oil, which were found to contain d-limonene and methyl jasmonate, demonstrated rapid and more effective insecticidal activity compared to the other EOs tested, which makes these two EOs promising alternatives to chemical insecticides.
研究领域:
农业昆虫学
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植物保护学
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天然产物化学
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农药学
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化学生态学
✓ 已验证
DOI
高影响因子
Plant Methods
·
2017
·Vol. 13
·期 1
·1-11
·
IF 3.51
Zhang Heng,Chen Zhenyi,Li Taihao,Chen Na,Xu Wenjie,Liu Shupeng
Background
Flos Chrysanthemi , as a part of Chinese culture for a long history, is valuable for not only environmental decoration but also the medicine and food additive. Due to their voluminously various breeds and extensive distributions worldwide, it is burdensome to make recognition and classification among numerous cultivars with conventional methods which still rest on the level of morphologic observation and description. As a fingerprint spectrum for parsing molecular information, surface-enhanced Raman scattering (SERS) could be a suitable candidate technique to characterize and distinguish the inter-cultivar differences at molecular level. Results SERS spectra were used to analyze the inter-cultivar differences among 26 cultivars of Chinese ornamental Flos Chrysanthemum . The characteristic peaks distribution patterns were abstracted from SERS spectra and varied from cultivars to cultivars. For the bands distributed in the pattern map, the similarities in general showed their commonality while in the finer scales, the deviations and especially the particular bands owned by few cultivars revealed their individualities. Since the Raman peaks could characterize specific chemical components, those diversity of patterns could indicate the inter-cultivar differences at the chemical level in fact. Conclusion In this paper, SERS technique is feasible for distinguishing the inter-cultivar differences among Flos Chrysanthemum . The Raman spectral library was built with SERS characteristic peak distribution patterns. A new method was proposed for Flos Chrysanthemum recognition and taxonomy.
研究领域:
植物学
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分析化学
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光谱学
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分子系统学
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中药鉴定
✓ 已验证
DOI
高影响因子
Natural Product Communications
·
2025
·
IF 1.4
Longwei Fang,Zhen Ni,Yanglei Shen,Feng Gao,La Yang,Nanling Liu,Zhuoma Duoji,Dongzhi Zhuoma,Wangdui Suolang,Zhuoma Basang
ObjectiveTo study the chemical constituents of Synotis solidaginea (SS) from Tibet. In our previous research, it was found that the extract of Synotis solidaginea stems has potential inhibitory activity against the proliferation of melanoma cells. Therefore, we hope to separate, extract and structurally identify the extracts of Synotis solidaginea stems, and determine the main components that exert potential inhibitory activity on the proliferation of melanoma cells.MethodsThe compounds were separated and purified using silica gel, polyamide, reversed phase C18 column chromatography, and preparative high performance liquid chromatography. The structure of each compound was identified based on the spectral data and literature comparisons. To verify the inhibitory activity of each component against melanoma cells, cell and enzyme assay were conducted.ResultsA total of 7 compounds were isolated from the dichloromethane extract of Synotis solidaginea. The irimofen lactone structure compound SS-1 and SS-5 were isolated from Synotis solidaginea for the first time. Among these, irimofin sesquiterpenoid compound SS-2 exhibited strong inhibitory activity against B16F10 of melanoma cells. Meanwhile, the iremorphene sesquiterpenoid SS-2 exhibited significant tyrosinase inhibitory activity, and the docking results explained the mechanism of its action.DiscussionSolidaginea has laid the foundation for the prevention and treatment of melanoma with the active ingredients of Tibetan medicine.
研究领域:
天然药物化学
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肿瘤学
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分子生物学
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药理学
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植物化学
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转化医学
✓ 已验证
DOI
高影响因子
Journal of Analytical Methods in Chemistry
·
2022
·Vol. 2022
·期 1
·2565494
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IF 2.594
Qiulong Zhao,Chunxue Wang,Jiaxin Cheng,Hui Yan,Ling Wang,Dawei Qian,Jinao Duan
According to the sixth edition of China’s “New Coronavirus Diagnosis and Treatment Plan (NCDTP),” ReDuNing injection (RDN) was firstly introduced to treat severe and critical COVID-19, whereas its combination with broad-spectrum antibiotics was suggested to take with extreme caution and full reasons. Therefore, we aim to describe the pharmacokinetics of seven active phytochemicals and semiquantification of nine relevant metabolites in ReDuNing injection (RDN) after combining with cefuroxime sodium (CNa) for injection in rat plasma. Male Sprague–Dawley rats were randomly assigned to six groups, and they were intravenously administered, respectively, with different prescriptions of RDN (2 mL/kg) and CNa (225 mg/kg). At different time points (0.03, 0.08, 0.17, 0.24, 0.33, 0.50, 0.67, 1, and 6 h) after administration, the drug concentrations of iridoids glycosides, organic acids, and metabolites in rat plasma were determined using ultrahigh-pressure liquid chromatography coupled with linear ion rap-orbitrap tandem mass spectrometry (UHPLC–LTQ–Orbitrap–MS), and main pharmacokinetic parameters were estimated by noncompartment model. The results showed that there were differences in pharmacokinetic parameters, AUC (0-t) , T 1/2 , C max , CL of iridoids glycosides, and organic acids, after the intravenous administration of the different combinations of RDN and CNa. Moreover, different combinations of the injections also resulted in different curves of relative changes of each metabolite. The obtained results suggested that RDN and CNa existed pharmacokinetic drug–herb interactions in rats. The findings not only lay the foundation for evaluating the safety of RDN injection combined with CNa but also make contributions to clinically applying RDN injection combined with CNa, which works potentially against severe forms of COVID-19.
研究领域:
药理学
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药物代谢
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中药学
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临床药学
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分析化学
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病毒学
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转化医学
✓ 已验证
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2023
·Vol. 415
·期 26
·6399-6410
·
IF 4.3
Jiang Maoyuan,Hua Shiyao,Zou Liang,Wang Yitao,Li Peng
Huangqi Guizhi Wuwu decoction (HGWD), as a classical formula, has been used for thousands of years in China. In this work, a comprehensive strategy was proposed for characterizing the chemical profile of HGWD based on online two-dimensional hydrophilic interaction and reversed-phase liquid chromatography coupled with hybrid linear ion trap-Orbitrap mass spectrometry (online HILIC × RP-ESI/HRMS/MS n ). The compounds in HGWD were first separated by the combined use of an XBridge amide column (150 × 4.6 mm, 3.5 μm) and Accucore C18 column (50 mm × 4.6 mm, 2.6 μm). Modulation with assistant technology, including trap columns and online dilution, was optimized and developed to decrease potential analyte loss and improve the resolution of the system. Subsequently, the accurate mass was determined by high-resolution Orbitrap and MS n fragment data by a hybrid linear ion trap (LTQ). In total, 170 chemical constituents were unambiguously identified or tentatively characterized in both positive and negative ion modes. Our study demonstrated that the proposed online HILIC × RP system coupled to the LTQ-Orbitrap MS platform is an efficient analytical technique for characterizing the chemical profile of multicomponent systems.
研究领域:
分析化学
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药物分析
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中医药学
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质谱分析
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色谱分析
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DOI
高影响因子
Chinese Journal of Natural Medicines
·
2025
·
IF 4.9
Yang Yu,Changliang Yao,Jianqing Zhang,Yong Huang,Shuai Yao,Hua Qu,Tong Zhang,Dean Guo
Aconiti Lateralis Radix Praeparata (Fuzi) represents a significant traditional Chinese medicine (TCM) that exhibits both notable pharmacological effects and toxicity. Various processing methods are implemented to reduce the toxicity of raw Fuzi by modifying its toxic and effective components, primarily diterpenoid alkaloids. To comprehensively analyze the chemical variations between different Fuzi products, ultra-high performance liquid chromatography-linear ion trap quadrupole Orbitrap mass spectrometry (UHPLC-LTQ-Orbitrap MS) was employed to systematically characterize Shengfuzi, Heishunpian and Baifupian. A total of 249 diterpenoid alkaloids present in Shengfuzi were identified, while only 111 and 61 in Heishunpian and Baifupian were detected respectively, indicating substantial differences among these products. An untargeted metabolomics approach combined with multivariate statistical analysis revealed 42 potential chemical markers. Through subsequent validation using 52 batches of commercial Heishunpian and Baifupian samples, 8 robust markers distinguishing these products were identified, including AC1-propanoic acid-3OH, HE-glucoside, HE-hydroxyvaleric acid-2OH, dihydrosphingosine, N -dodecoxycarbonylvaline and three unknown compounds. Additionally, the MS imaging (MSI) technique was utilized to visualize the spatial distribution of chemical constituents in raw Fuzi, revealing how different processing procedures affect the chemical variations between Heishunpian and Baifupian. The distribution patterns of different diterpenoid alkaloid subtypes partially explained the chemical differences among products. This research provides valuable insights into the material basis for future investigations of different Fuzi products.
研究领域:
分析化学
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植物化学
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代谢组学
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质谱学
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中药学
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生药学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）