Advanced Therapeutics
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2018
·Vol. 1
·期 1
·1800005
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IF 0
Jiayi Huang,Yi Hou,Tiancong Ma,Peisen Zhang,Yingying Li,Chunyan Liu,Boyuan Zhang,Shanshan Qiu,Jianfeng Zeng,Mingyuan Gao
Rare-earth (RE)-based nanoparticles have exhibited broad potential applications in the biomedical field owing to their unique magnetic and optical properties. But until now, there has been no reliable histochemical staining method developed for probing the distribution of RE-based nanoparticles in different organs and tissues, which is imperative for evaluating the targeting ability and safety profiles of RE-based nanoparticles used as the diagnostic probe and the therapeutic agent. Herein, the authors propose a novel and effective staining approach for identifying RE-based nanoprobes in tissues through unique chromogenic reactions between Chlorophosphonazo III and RE 3+ , which is independent of the element type. More importantly, a staining color analysis method based on the specific RGB range-selective integration of the stained images is developed for establishing a quantitative relationship between the optical density and RE 3+ concentration in the tissue slices.
研究领域:
生物医学工程
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病理学
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材料科学
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产品关联
DOI
SENSORS AND ACTUATORS B-CHEMICAL
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2018
·
IF 5.667
Leina Dou,Tong Bu,Wentao Zhang,Bingxin Zhao,Qingfeng Yang,Lunjie Huang,Sihang Li,Chengyuan Yang,Jianlong Wang,Daohong Zhang
The nanomaterials based lateral flow immunoassays (LFIAs) have been widely used for on-site detection of small molecules contaminants. Nevertheless, their practical applications are often constrained by the complicated materials synthesis and surface modification process of nanomaterials. Herein, inspired by chemical-staining, a crystal violet (CV) staining based LFIAs was innovatively developed for the first time to analyze small molecules contaminant with significantly simplified behaviour and surpassing repeatability. This nanomaterials-free technique overcomes the intrinsic limitations of nanomaterials, owing to the superior protein dye property of CV. To demonstrate the feasibility of the unique strategy, furazolidone (FZD) was used as a model analyte, results showed detection limits of as low as 7 and 1.53 ng mL −1 by visual observation and quantitative analysis, respectively. Finally, to validate its applicability in actual conditions, the platform is well applied in three kinds of food samples (shrimp, fish and pork). Furthermore, as the detection principle is based on the molecular recognition between antigen and antibody, our method may find general applications by staining other antibodies against other target analytes.
研究领域:
分析化学
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食品安全
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生物医学
✓ 已验证
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2024
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IF 13.3
Jingke Xu,Jing Zhou,Kai Liu,Tong Bu,Leina Dou,Rui Shu,Sijie Liu,Shaochi Wang,Xuechi Yin,Daohong Zhang,Ruiling Zhang,Jie Cheng,Jianlong Wang
Nanomaterials-based lateral flow immunoassays (LFIAs) have gained great commercial success in small molecule monitoring, while a bigger picture of this technique can be torn up by sensitivity issues, resulting from the difficulties in potentiating bio functionality of nanoprobes laden with antibodies (Abs). Herein, a novel one-to-one recognition marker, colored half-Ab (cHAb) by a facile “chemical-staining technique”, was engineered into LFIAs vehicle to leverage its high luminance capability and Fab independent binding behavior of HAb. The designed cHAb not only simplified the preparation process of probes using dyes, but also effectively improved the unique recognition ability of the target via HAb, thereby abandoning the inherent limitations of traditional nanomaterials and Abs, and imparting a high sensitivity. Proof-of-concept was proved by introducing coomassie bright blue R-250 (CBBR) stained HAb as examples to detect clenbuterol (CLE). The superior detection advantage of HAb was first verified by indirect competitive ELISA (ic-ELISA), demonstrating that HAb can improve the sensitivity by about 3.5 folds compared with Ab. In addition, a cHAb-based LFIA (HAb-CLFIA) was developed, proving that applying HAb can improve the detection sensitivity of about 1.8-folds whilst still exhibiting a wide linear range coverage of 80 % of the cut-off value. Spiked honey sample testing can detect CLE at least 10 μg kg −1 , providing the same sensitivity to water samples, validating that HAb-CLFIA is equipped with good applicability. Importantly, HAb combined with chemical-staining technology exhibits great potential for next-generation LFIA for small molecule contaminants detection.
研究领域:
生物化学
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纳米材料科学
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分析化学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
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2022
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IF 9.231
Yuechun Li,Han Zhang,Zhaowen Cui,Sijie Liu,Jingke Xu,Conghui Jia,Yaqian Chen,Lulu Wang,Jing Sun,Daohong Zhang,Mingqiang Zhu,Jianlong Wang
Exploring a novel strategy for strengthening the catalytic activity of enzyme facilitates the development of a sensitive enzyme-linked immunosorbent assay (ELISA). Herein, a chemical staining (CS) strategy was firstly discovered to possess the ability to directly improve the catalytic activity of horseradish peroxidase. Based on this discovery, coomassie brilliant blue was introduced into ELISA to establish a CS enhanced ELISA (CS-ELISA) to detect clenbuterol (CL) by simply staining monoclonal antibodies. Satisfactorily, the most important analytical parameters of CS-ELISA, including sensitivity (0.074 ng mL −1 ) and linear range (0.2–2 ng mL −1 ) were all improving 2-folds compared with conventional ELISA. Moreover, the CS-ELISA shows good applicability in the detection of CL in pork tenderloin samples. The proposed CS-ELISA shows various advantages, such as cost-effective, easily accessible, enhanced catalytic activity of enzyme, higher sensitivity, and broader linear range, providing a new insight into enhanced ELISA for food safety.
研究领域:
食品安全
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分析化学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
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2025
·
IF 4.6
Shaoping Zhu,Li Hu,Jiaxin He,Jun Feng,Caihong Li,Defeng Xu
This study aims to establish a histological protocol to visualize the process of structural protein degradation in shrimp Litopenaeus vannamei during refrigeration. After fixed in Carnoy’s fixative for 15 h at room temperature and combined with Masson’s trichrome staining, the structural components of myofibrils and collagen were effectively visualized and the pathological analysis revealed the widened gaps between muscle bundles and the disrupted myofibrils were observed. Moreover, the lighter staining in collagen component was observed during the cold storage, indicating its progressive reduction in content. Quantitatively, the myofibril cross-sectional and longitudinal volume fractions dropped from (91.03 ± 1.37) % and (93.39 ± 0.91 %) to (41.14 ± 3.78) % and (24.36 ± 3.67) % (Day 5, P < 0.01), respectively. Meanwhile, the collagen and myofibril areas were negatively correlated with the parameters of hardness and elasticity ( P < 0.05), confirming their role in inducing texture quality deterioration. Collectively, by visualizing the degradation process of structural components collagen and myofibril in shrimp muscle during refrigeration, the association between structural proteins degradation and texture deterioration was identified. This study identifies a potential biomarker of early muscle softening in refrigerated shrimp L. vannamei and provides a solid foundation for its industrial application in quality control of shrimp during preservation.
研究领域:
食品科学
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海洋生物学
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组织学
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生物化学
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采后生理学
✓ 已验证
DOI
高影响因子
EXPERIMENTAL BIOLOGY AND MEDICINE
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2020
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IF 3.139
Jiaqi Li,Yongguang Zhang,Zhimin Yang,Jingxian Zhang,Ren Lin,Daoshu Luo
Schwann cell and nerve conduit are crucial for nerve regeneration and re-myelination after peripheral nerves injury. To investigate the effects of Salidroside on autogenous epimysium conduit mixed with BD matrigel and RSC96 Schwann cells on an animal model with 5 mm sciatic nerve defect injury in rat, motor function, muscle reinnervation, immunohistochemical staining, retrograded tracing and Western blot were used in this study. The results showed that Salidroside enhanced the compound effects of epimysium conduit mixed with BD matrigel and RSC96 Schwann cells to improve the sciatic functional index and the gastrocnemius muscle weight ratio, which were better than EPM group at 8 weeks and 12 weeks post operation. Immunofluorescence and Western blot results of P75NTR showed that Salidroside improved the sciatic nerve regeneration, and retrograded tracing of CTB-Alexa 488 also supported that Salidroside was better to promote CTB tracer transporting from the distal nerve defect to the ipsilateral dorsal root ganglion and ventral horn of L3-L5 spinal cord on post-operation 8 weeks and 12 weeks. Our results demonstrated that Salidroside improved the effect of autogenous epimysium conduit mixed with BD matrigel and RSC96 Schwann cells on sciatic nerve regeneration in our study.Impact statementPeripheral nerve injury and regeneration remain a major challenge. Although nerve conduit and Schwann cells have been used to study the nerve regeneration, our results demonstrated that Salidroside improved the regenerative effect in a rat model with sciatic nerve injury model, following a combined application of autogenous epimysium conduit mixed with Schwann cells. Different concentrations of Salidroside combining autogenous epimysium conduit and Schwann cells were applied to compare the epimysium conduit group and the epimysium conduit combining Schwann cells group. Based on the results of motor function and muscle reinnervation evaluation, as well as neuronal tracing and expression of P75NTR, our study for the first time suggests that Salidroside may improve the regeneration effect on the sciatic nerve following a combined application of epimysium conduit and RSC96 Schwann cells in rats.
研究领域:
神经科学
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再生医学
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药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nanoscale
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2024
·
IF 5.8
Xinru Kong,Yanxi Yang,Xueli Ren,Yandai Lin,Yu Shi,Zhe Liu
The concept of combining external medical stimuli with internal functional biomaterials to serve cancer-oriented treatments is emergingly developed. Optical and acoustical activations have shown special promises as non-invasive regulation modalities in cancer treatment and intervention. It is always challenging to leverage their contributions between optical and acoustical stimuli and find the appropriate biomaterials to optimally match them. Herein, a type of hybrid nanomicelles (ICG@PEP@HA) containing ICG as photo/sonosensitizers, an amphiphilic peptide for membrane penetration and hyaluronic acid for cluster determinant 44 (CD44) targeting were fabricated. Triggered by external stimuli of laser and US irradiations, their photo/sonothermal performances, in vitro reactive oxygen species (ROS) production capabilities and tumor-targeting efficiency have been systematically evaluated. It was interestingly found that the external stimulus of laser induced more quantity of ROS which resulted in a significant cell apoptosis and tumor growth inhibition in the presence of ICG@PEP@HA. The individual analysis and corresponding rationales have been investigated. In the meanwhile, these hybrid nanomicelles were administered into the MDA-MB-231 tumor-bearing nude mice for PDT and SDT therapies and their biocompatibility assessment, and a PDT prevailing efficacy and reliable bio-safety have been evidenced based on the hematological analysis and histochemical stainings. In summary, this study has validated a novel pathway to utilize these hybrid nanomicelles for laser/US-triggered localized tumor treatments, and the treatment efficiency may be leveraged by different external stimuli sources. It is also expected to give birth to full accessibility to clinical translations for human cancer treatments by means of as-reported laser/US-nanomicelles combination strategy.
研究领域:
纳米技术
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癌症治疗
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生物医学工程
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药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2024
·
IF 13.3
Lei Liang,Hongda Wang,Linfa Li,Ding Lin,Bingyan Guo,Mengmeng Yao,Zhijian Wei,Hong Zhang,Junjie Li
Stomach trauma repair remains a paramount concern in the biomedical field. Despite numerous studies into the creation of gastric hydrogel patches, many exhibit inadequate wet-state adhesion or problematic double-sided adhesion that can inadvertently adhere to surrounding tissues. Consequently, asymmetric adhesive hydrogels are one of the directions for the development of suture-less sealing wound patches for the stomach trauma. However, existing asymmetric patches often entail intricate fabrication processes, challenging their scalability for industrial production. In this study, we propose a novel and universal preparation method for fabricating asymmetric adhesive hydrogel patches for favorable suture-less sealing gastric repair based on the existing high adhesive strength hydrogel patch research. This is followed by a thorough examination of its adhesion qualities using various adhesion tests. MTS assay and AO staining method are used to confirm the cytocompatibility. The bursting test is used to simulate in vitro stomach trauma. Subsequently, a rat gastric trauma repair model is performed to mimic its application in actual surgery, and its excellent repair effect is confirmed by immunohistochemical staining and immunofluorescence staining, which verifies the potential of asymmetric adhesive hydrogel patches prepared by microparticle deposition induced method in biomedical field.
研究领域:
生物医学工程
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组织工程
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材料科学
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外科技术
✓ 已验证
产品关联
DOI
高影响因子
PHYTOMEDICINE
·
2023
·
IF 7.9
Shuai Chen,Jie Jin,Ziqing Xu,Huawei Han,Lan Wu,Zhiwei Li
Background Catalpol , a major active component of the Chinese herb Rehmannia glutinosa , possesses various pharmacological benefits, including anti-inflammatory, antidiabetic , and antitumor properties. Recent studies have reported that catalpol can attenuate bone loss and enhance bone formation. Nevertheless, the molecular mechanisms underlying its effects on osteoporosis pathogenesis remain unclear. Purpose We investigated whether catalpol had a protective effect against postmenopausal osteoporosis (PMOP) and explored its exact mechanism of action. Methods Seventy-two rats were randomly divided into six groups: sham, model, low-dose catalpol (5 mg/kg/day), medium-dose catalpol (10 mg/kg/day), high-dose catalpol (20 mg/kg/day), and positive control (alendronate, 2.5 mg/kg). In this experiment, a ovariectomy was performed to establish a female rat model of PMOP. After 12 weeks of gavage, micro-computed tomography (micro-CT) and histochemical staining were performed to evaluate bone mass, bone microstructure and histological parameters. Furthermore, RAW 264.7 cells were induced by RANKL to form mature osteoclasts to investigate the effect of catalpol on osteoclast differentiation and apoptosis in vitro . Additionally, the osteoclast apoptosis-related proteins of Sirt6 , ERα, FasL , NFATc1, cleaved-caspase 8, cleaved-caspase 3, and Bax were assessed using western blotting . The expressions of NFATc1, Ctsk, Oscar , and Trap were quantified using RT-qPCR. The apoptotic rate of the osteoclasts was determined using flow cytometry. Sirt6 knockdown was performed using siRNA gene silencing in experiments to investigate its role in catalpol-mediated osteoclast apoptosis. The deacetylation of ERα in osteoclasts was tested via co-immunoprecipitation. Results Catalpol (10 and 20 mg/kg) and alendronate (2.5 mg/kg) could significantly improve bone mineral density (BMD) and microstructure and decrease osteoclast density in ovariectomized (OVX) rats. In addition, catalpol (10 and 20 mg/kg) upregulated the expression of Sirt6, ERα, FasL, cleaved-caspase 8, cleaved-caspase 3, Bax, and downregulated the expression of NFATc1, Ctsk, Oscar, Trap both in vivo and in vitro . Catalpol also promoted ERα deacetylation and stabilized ERα protein to enhance the expression of FasL. In addition, Sirt6 knockdown by siRNA prevented ERα deacetylation and eliminated catalpol-mediated osteoclast apoptosis. Conclusions The present study demonstrated that catalpol prevents estrogen deficiency-induced osteoporosis by promoting osteoclast apoptosis via the Sirt6-ERα-FasL axis. These findings revealed a novel molecular mechanism underpinning the impact of catalpol in the progression of osteoporosis and provided novel insights into the treatment of osteoporosis.
研究领域:
药理学
·
骨骼生物学
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分子生物学
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内分泌学
✓ 已验证
DOI
PubMed
高影响因子
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