Methods in Cell Biology
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2020
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IF 1.441
Dattatrya Shetti,Bae Hoon Lee
Animal models for studying human breast cancer carcinogenesis and testing drug candidates on human breast cancer have extensively been proposed. Especially, chemically induced breast tumor models have been used because they can mimic the progression of clinical cancer from the beginning and can be generated with a facile procedure. Pristane is a hydrocarbon oil that is used as a chemical carcinogen to induce tumorigenesis in mice as well as arthritis and lupus nephritis in rats. In only a few studies, pristane-induced breast cancer models have been reported. This chapter is designated to describe pristane-induced mammary carcinoma models. Here, we provide a protocol for generating pristane-induced breast tumors in mice models for analyzing and for testing potential therapeutics on them. The given protocol can be applied to other animal models with some changes.
研究领域:
肿瘤学
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药理学
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DOI
PubMed
高影响因子
JOURNAL OF ORGANOMETALLIC CHEMISTRY
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2015
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IF 2.173
Lei Xue,Liguo Li,Shengyu Feng,Hongzhi Liu
Starting from octavinylsilsesquioxane (OVS), several multifunctional cage silsesquioxanes were successfully prepared by the photo–induced thiol–ene reaction with the corresponding commercially available thiols. These cage compounds were fully characterized by FTIR , NMR, ESI–MS and element analysis .
研究领域:
材料科学
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化学工程
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产品关联
DOI
高影响因子
MOLECULAR CELL
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2024
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IF 14.5
Xiaoding Ma,Jianli Yin,Longliang Qiao,Hang Wan,Xingwan Liu,Yang Zhou,Jiali Wu,Lingxue Niu,Min Wu,Xinyi Wang,Haifeng Ye
Myriad physiological and pathogenic processes are governed by protein levels and modifications. Controlled protein activity perturbation is essential to studying protein function in cells and animals. Based on Trim-Away technology, we screened for truncation variants of E3 ubiquitinase Trim21 with elevated efficiency (ΔTrim21) and developed multiple ΔTrim21-based targeted protein-degradation systems (ΔTrim-TPD) that can be transfected into host cells. Three ΔTrim-TPD variants are developed to enable chemical and light-triggered programmable activation of TPD in cells and animals. Specifically, we used ΔTrim-TPD for (1) red-light-triggered inhibition of HSV-1 virus proliferation by degrading the packaging protein gD, (2) for chemical-triggered control of the activity of Cas9/dCas9 protein for gene editing, and (3) for blue-light-triggered degradation of two tumor-associated proteins for spatiotemporal inhibition of melanoma tumor growth in mice. Our study demonstrates that multiple ΔTrim21-based controllable TPD systems provide powerful tools for basic biology research and highlight their potential biomedical applications.
研究领域:
分子生物学
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细胞生物学
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生物医学工程
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DOI
PubMed
高影响因子
Journal of Physical Chemistry C
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2018
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IF 4.484
Peng Peng,Lihang Li,Wei Guo,Zhuang Hui,Jian Fu,Chao Jin,Yangai Liu,Ying Zhu
Flexible electrodes have broad applications in microelectronics. In this work, flexible electrodes were fabricated through chemically induced joining of silver nanoparticles at room temperature. After the joining process, the resistance of the silver track decreased by 8 orders of magnitude. The joining parameters were systematically investigated including the concentration of the chemical solution, sintering time, and drying temperature. This chemical method achieved the same effect at room temperature as compared to the methods using thermal sintering. The changes of the microstructure and the surface composition of silver nanoparticles were characterized to better understand the nanojoining process at room temperature.
研究领域:
材料科学
·
微电子学
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产品关联
DOI
高影响因子
APPLIED CATALYSIS B-ENVIRONMENTAL
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2018
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IF 11.698
Yanan Liu,Chenglin Miao,Pengfei Yang,Yufei He,Junting Feng,Dianqing Li
The ultrathin TiO 2 nanosheets (TiO 2 -U) with abundant defects were successfully fabricated as substrates to support well-dispersed Pt nanoparticles with low metal loading by photochemical route (P), with impregnated Pt/TiO 2 -U, Pt/TiO 2 -bulk and photoreduced Pt/TiO 2 -bulk as control samples, to focus on studying the inductive effect of oxygen vacancy-rich ultrathin TiO 2 on highly dispersed Pt nanoparticles, and their synergetic promotional effect for CO 2 photoreduction. As expected, P-Pt/TiO 2 -U exhibited excellent photocatalytic efficiency for the selective conversion of CO 2 into CH 4 and CO. The ultrathin TiO 2 nanosheets with a large number of low coordinative sites and ultralarge surface area promoted the rate of electron-transfer. The ultrafine Pt nanoparticles induced by photochemical strategy facilitated the efficient separation of electron–hole pairs. Furthermore, the synergy of metal and support improved the adsorption ability of CO 2 . These three factors were considered to drive jointly the enhancement of catalytic performance in this system. This work offers deep insights for the design of highly efficient catalysts with coordinatively unsaturated sites for CO 2 photoconversion in the presence of H 2 O.
研究领域:
材料科学
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环境科学
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产品关联
DOI
高影响因子
CELL PROLIFERATION
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2023
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IF 8.5
Yun Lv,Ziyan Rao,Lulu Liu,Jun Jia,Chenyang Wu,Jun Xu,Yuanyuan Du,Yinan Liu,Bei Liu,Jihang Shi,Guangya Li,Dongyu Zhao,Hongkui Deng
Derivation of human hepatocytes from pluripotent stem cells in vitro has important applications including cell therapy and drug discovery. However, the differentiation of pluripotent stem cells into hepatocytes in vitro was not well recapitulated the development of liver. Here, we developed a differentiation protocol by mimicking the two-stage development of hepatoblasts, which permits the efficient generation of hepatic progenitor cells from chemically induced pluripotent stem cells (hCiPSCs). Single-cell RNA sequencing (scRNA-seq) indicates the similarity between hepatoblasts differentiated in vitro and in vivo. Moreover, hCiPSC-derived hepatic progenitor cells can further differentiate into hepatocytes that are similar to primary human hepatocytes with respect to gene expression and key hepatic functions. Our results demonstrate the feasibility of generating hepatic progenitor cells and hepatocytes from hCiPSCs with high efficiency and set the foundation for broad translational applications of hCiPSC-derived hepatocytes.
研究领域:
干细胞研究
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分子生物学
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再生医学
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DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2025
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IF 6.9
Yuzhe Liu,Qi Li,Yuhan Liang,Junhao Liang,Wenliang Feng,Bin Wu,XuLei Sui,Zhenbo Wang
The inherently low active site accessibility of single-atom Fe-N-C catalysts severely compromises their catalytic activity relative to Pt-based catalysts, thereby hindering their practical implementation in critical applications. This work systematically investigates the synergistic effects of physical confinement and chemical induced growth strategy on optimizing active site accessibility of Fe-N-C catalysts for oxygen reduction reaction. Through a physical confinement strategy, the catalyst particle size is successfully reduced from the micrometer scale to the nanometer scale. Subsequently, by employing chemical-induced growth, the particle size is precisely controlled within the range of 623 nm to 421 nm, simultaneously establishing an optimal balance between the catalyst particle size threshold (420 nm) and the ordered macroporous framework. This approach maximized the accessibility of active sites and significantly enhanced the catalytic activity of the Fe-N-C catalyst. The optimized catalyst (OM-Fe-NC-4) exhibits outstanding performance, achieving a remarkable half-wave potential of 0.888 V, surpassing both commercial Pt/C and most state-of-the-art Fe-N-C catalysts. Additionally, it demonstrates excellent four-electron selectivity and delivers a kinetic current density of 14.7 mA cm −2 at 0.85 V in acidic medium. This dual-dimensional modulation strategy offers a paradigm for enhancing the accessibility of active sites for non-precious metal catalysts.
研究领域:
材料科学
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催化化学
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能源科学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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2023
·Vol. 24
·期 21
·15722
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IF 5.6
Wanwan Wang,Haiyun Rui,Lei Yu,Nuo Jin,Wan Liu,Chen Guo,Yumeng Cheng,Yonggen Lou
Chemical elicitors can increase plant defense against herbivorous insects and pathogens. The use of synthetic chemical elicitors is likely to be an alternative to traditional pesticides for crop pest control. However, only a few synthetic chemicals are reported to protect plants by regulating signaling pathways, increasing the levels of defense metabolites and interfering with insect feeding. Here, we found that the exogenous application of a phenoxycarboxylic compound, 4-chlorophenoxyacetic acid (4-CPA), can induce chemical defenses to protect rice plants from white-backed planthoppers (WBPH,Sogatella furcifera). Four-CPA was rapidly taken up by plant roots and degraded to 4-chlorophenol (4-CP). Four-CPA treatment modulated the activity of peroxidase (POD) and directly induced the deposition of lignin-like polymers using hydrogen peroxide (H2O2) as the electron acceptor. The polymers, which are thought to prevent the planthopper’s stylet from reaching the phloem, were broken down by WBPH nymphs. Meanwhile, 4-CPA increased the levels of flavonoids and phenolamines (PAs). The increased flavonoids and PAs, together with the degradation product of the polymers, avoided nymphal feeding and prolonged the nymphal period for 1 day. These results indicate that 4-CPA has the potential to be used as a chemical elicitor to protect rice from planthoppers. Moreover, these findings also open a pathway for molecule structure design of phenoxycarboxylic compounds as chemical elicitors.Keywords:chemical elicitor;4-chlorophenoxyacetic acid;lignin-like polymers;white-backed planthoppers;rice;chemical defense
研究领域:
植物科学
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昆虫学
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农业化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2022
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IF 9.965
Chunli Li,Meng Li,Huiting Xu,Fan Zhao,Siqi Gong,Honghai Wang,Junjie Qi,Zhiying Wang,Xiaobin Fan,Wenchao Peng,Jiapeng Liu
Aqueous zinc-ion batteries receive more and more attentions on account of their low cost, high theoretical density and inherent safety. Nevertheless, the lack of suitable cathode materials with excellent performance still severely impedes the development of aqueous zinc-ion batteries. Herein, an in-situ electrochemical induction strategy is developed to prepare hollow nanotube-like amorphous vanadium oxide and carbon (a-V 2 O 5 @C) hybrid and its electrochemical performance is investigated comprehensively as cathode materials for aqueous zinc-ion batteries. Benefitting from the unique amorphous structure of V 2 O 5 and intimate contact between amorphous V 2 O 5 and carbon, the a-V 2 O 5 @C hybrid possess the abundant ion storage sites, isotropic ion diffusion routes and excellent conductivity. As a result, the a-V 2 O 5 @C hybrid cathode shows outstanding specific capacity of 448 mAh g −1 at 0.15 A g −1 . Impressively, the a-V 2 O 5 @C hybrid cathode exhibits superior cycling stability, even when cycling at high current density of 10 A g −1 , that the 96.5% specific capacity retention can be gained over 1500 cycles, corresponding to an average specific capacity loss of only 0.0023% per cycle. Furthermore, the mechanism involved is illustrated by systematical characterizations. Therefore, this work affords a new way for developing high-performance cathode materials for aqueous zinc-ion batteries.
研究领域:
材料科学
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电化学
·
能源存储
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DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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2019
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IF 2.813
Nadeem Muhammad,Dandan Guo,Yun Zhang,Azeem Intisar,Qamar Subhani,Muhammad Abdul Qadir,Hairong Cui
Analysis of acidic pharmaceuticals in complex biological samples is a challenging and formidable task due to the existence of interfering constituents within the sample matrices. Therefore, in order to avoid analytical column clogging and suppression/enhancement of signals of the analyte of interest, herein a simple, cost-effective and quick online ion chromatography based clean-up setup was introduced. This system was further coupled with a cost-effective homemade photochemically induced fluorimetric (PIF) setup for direct online conversion of non-fluorescent acidic pharmaceutical drugs into their respective fluorescent species. This advantageous system was favorably applied for the determination of four non-fluorescent acidic compounds in two complex biological samples (human serum and oral fluid) with minimum labor and organic solvent consumption. At optimized conditions, the developed method has shown good sensitivity, selectivity, satisfactory recoveries (88.68–102.14%) and low limits of detection (0.35–8.10 μg/L) with minimum or zero matrix effect.
研究领域:
分析化学
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药学
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产品关联
DOI
PubMed
高影响因子
Small
·
2024
·
IF 13
Yingyan Ma,Yuan Ha,Liangqiang Chen,Ziqi An,Linzhuang Xing,Zhenni Wang,Zhimin Li
Efficient and affordable price bifunctional electrocatalysts based on transition metal oxides for oxygen and hydrogen evolution reactions have a balanced efficiency, but it remains a significant challenge to control their activity and durability. Herein, a trace Ru (0.74 wt.%) decorated ultrathin CoOOH nanosheets (≈4 nm) supported on the surface of nickel foam (Ru/CoOOH@NF) is rationally designed via an electrochemically induced strategy to effectively drive the electrolysis of alkaline overall water splitting. The as-synthesized Ru/CoOOH@NF electrocatalysts integrate the advantages of a large number of different HER (Ru nanoclusters) and OER (CoOOH nanosheets) active sites as well as strong in-suit structure stability, thereby exhibiting exceptional catalytic activity. In particular, the ultra-low overpotential of the HER (36 mV) and the OER (264 mV) are implemented to achieve 10 mA cm −2 . Experimental and theoretical calculations also reveal that Ru/CoOOH@NF possesses high intrinsic conductivity, which facilitates electron release from H 2 O and H-OH bond breakage and accelerates electron/mass transfer by regulating the charge distribution. This work provides a new avenue for the rational design of low-cost and high-activity bifunctional electrocatalysts for large-scale water-splitting technology and expects to help contribute to the creation of various hybrid electrocatalysts.
研究领域:
电化学
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材料科学
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催化
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能源转换与存储
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产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2025
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IF 8.2
Yancheng Chen,Jianxin Ou,Shanyong Guo,Xuanbing Chen,Yihan Wen,Jiayuan Chen,Derong Lu,Hui Zhang,Hao Qian,Lifeng Cai,Huabin Kong,Hongwei Chen
Crystal repair strategies are commonly used to extend the cycling life of electrode materials, but their effectiveness is often limited and may introduce defects. In this study, we propose an electrochemically induced phase transformation approach to complement traditional repair strategies. This technique regenerates decayed α-phase V2O5 from deteriorated lithium-ion batteries by not only repairing defects but also forming stable bilayer structure crystals for excellent Zn2+ storage. By controlling the stimulated current, the phase transformation kinetics can be precisely tuned with the assistance of interlayer H2O. The reconfigured V2O5 achieves a remarkable Zn2+ areal capacity of 6.5 mA h cm–2 in aqueous zinc-ion batteries (ZIBs), with a cycling over 1500 cycles, outperforming ZIBs made from common fresh V2O5.
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DOI
PubMed
高影响因子
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