Chemistry-Switzerland
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2021
·Vol. 3
·期 4
·1113-1125
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IF 0
Yamin Zhao,Lujun Zhang,Xiaoxue Zhai,Qian Liu,Lebing Sun,Mengshi Liu,Lili An,Liang Xian,Ping Zhang,Lihua Chen
Polymer microspheres with inter-connecting pores are widely used as microsphere materials. In the study, the ethyl cellulose microspheres (ECM) were prepared by using the solvent-evaporation method. Based on that, a method for the separation and purification of benzoic acid from crude extract ofCyclocarya paliuruswas established by the ECM and high performance liquid chromatography (HPLC). The ECM after the sorption equilibrium was desorbed by using 40% methanol as the analytical solvent. The content of benzoic acid in eluent is up to 0.0216 mg/mL, and the benzoic acid can be obtained with a high purity of 82.22%. Furthermore, the adsorption-desorption behavior of benzoic acid onto ECM was investigated. The results of adsorption kinetics of benzoic acid showed that the adsorption followed the pseudo-first-order kinetic model. The ECM was characterized by using Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), scanning electron microscope (SEM), and X-ray diffraction (XRD). The results showed that the ECM has a high adsorption property due to its more porous structure, phenolic hydroxyl group, and other oxygen-containing functional groups. This method and the ECM can be used stably, continuously, and efficiently to purify the benzoic acid from the methanol extract ofC. paliuruson a large scale.Keywords:ethyl cellulose microspheres (ECM);HPLC;adsorption-desorption;benzoic acid;Cyclocarya paliurus
研究领域:
植物化学
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分离纯化技术
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DOI
INDUSTRIAL CROPS AND PRODUCTS
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2022
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IF 6.449
Meiling Sun,Zhenghui Luo,Qi Yang,Chunxu Huang,Yu Kuang,Mei Kang,Jie Liu
Methicillin-resistant Staphylococcus aureus (MRSA) has evolved to tolerate almost all known β-lactam antibiotics, and been exhibiting resistance to vancomycin . Developing new drugs against the MRSA infection are urgently needed. Natural resource is enriched with antimicrobial molecules. Here, we report an extract from leaves of Cyclocarya paliurus (ECPL), a species of tree native to China and being traditionally cultivated as medicinal plant , which shows a broad spectrum of bacteriostatic and bactericidal effects at MIC 50 15.99 ± 0.55 μg/ml against methicillin-susceptible and -resistant S. aureus . The ECPL suppresses bacterial expression of FtsZ at both transcriptional and protein levels, inhibits its GTPase activity, blocks the assembly dynamics of FtsZ, and disrupts the formation of Z-ring. The bacteria cells fail to divide, enlarge initially, and lyse eventually. In mouse model, the ECPL facilitates skin wound healing with MRSA infection as effective as vancomycin. Our study unveils the potential of identifying novel molecule in ECPL for development of new class of antibiotic against both methicillin-susceptible and -resistant S. aureus , and support domestication of the C. paliurus growing from wild to cultivated, and further scale up the cultivation into industrialized farm for sustainable harvest for pharmaceutic interest.
研究领域:
微生物学
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药理学
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植物科学
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传染病学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2019
·
IF 4.784
Xiaojiang Wang,Xiaoliang Zhao,Youjing Lv,Minghua Hu,Luodi Fan,Qinying Li,Chao Cai,Guoyun Li,Guangli Yu
A water soluble polysaccharide CP-III was extracted and purified from Cyclocarya paliurus . CP-III is identified as a novel pectin-like polysaccharide with molecular weight ( Mw ) of 72.7 kDa. The structural features of CP-III were characterized by methylation and nuclear magnetic resonance (NMR) spectroscopy. Its depolymerized fragments were analyzed by hydrophilic interaction chromatography-Fourier transform mass spectrometry (HILIC-FTMS). The main chain of CP-III is composed of →4)GalA p (α1 → and →2)Rha p (α1 → 4)GalA p (α1→, repeatedly. The residue of →4)Gal p (β1 → and →5)Ara f (α1 → alternately exists on the O -4 of partial →2)Rha p (α1 → residues as side chains. On the O -3 of sectional →5)Ara f (α1 → residues is a secondary branch assembled by →3)Ara f (α1→. Moreover, on the non-reducing terminus of →4)Gal p (β1 → occasionally have an →5)Ara f (α1 → chain. Surprisingly, a sub-branch constructed by →6)Hex p (β1 → with a galacturonate or methyl galacturonate exists on the O -3 of certain →4)Gal p (β1 → residues in the non-reducing terminus. In addition, a terminal Xyl is located on the O -3 of fractional →4)GalA p (β1 → residue. The highly branched polysaccharide CP-III with high water solubility can be used as food supplement and medicinal carrier in the future.
研究领域:
植物学
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药学
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食品科学
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产品关联
DOI
PubMed
高影响因子
Journal of Functional Foods
·
2023
·
IF 5.6
Rui Gui,Yi-Kun Wang,Jian-Ping Wu,Gui-Ming Deng,Fei Cheng,Hong-Liang Zeng,Pu-Hua Zeng,Hong-Ping Long,Wei Zhang,Xi-Fan Wei,Wen-Xuan Wang,Gang-Zhi Zhu,Wei-Qiong Ren,Zu-Hui Chen,Xiao-Ai He,Kang-Ping Xu
Cyclocarya paliurus , as an important edible and medicinal plant, its effects and mechanisms on hyperuricemic nephropathy remain unclear. Herein, we investigated the hypouricemic and nephroprotective effects of the water extracts of C. paliurus leaves (CPE) in a hyperuricemic nephropathy rat model induced by adenine and ethambutol. The results showed that CPE could significantly decrease plasma uric acid (PUA) and urea nitrogen (PUN), as well as reduce renal fibrosis in the hyperuricemic nephropathy rat model. Plasma metabolomics indicated that CPE improved the disordered arachidonic acid metabolism. Meanwhile, CPE reduced renal inflammation via inhibition of COX--2. Moreover, CPE could improve disturbed purine metabolism by inhibiting the hepatic xanthine oxidase (XOD), reversing the renal urate transporter 1 (URAT1) and organic anion transporter 1 (OAT1). Collectively, this study demonstrated that CPE could exhibit hypouricemic effect by improving purine metabolism, and attenuate kidney injury by improving arachidonic acid metabolism and alleviating kidney inflammation.
研究领域:
药理学
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肾病学
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代谢组学
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DOI
高影响因子
危险品化学品经营许可证（带存储）