SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Chunsong Lu,Jie Liu,Lanlan Gan,Xiaoming Yang
Distinguishing the isomers is considered to be challenging, and shows the great importance in the biochemical and pharmaceutical fields. Herein, the blue carbon dots (CDs) were successfully raised on the basis of a facile hydrothermal way, since Cryptococcus served as the carbon source. Importantly, the as-prepared CDs were employed to achieve the differentiation and detection of two benzenediols. To be specific, the fluorescence of CDs was enhanced once m -benzenediol (resorcinol) was added, while introducing p -benzenediol (hydroquinone) lead to their fluorescence quenching. Towards the mechanism, the addition of m -benzenediol promoted the formation of hydrogen bonds between m -benzenediol and CDs, and thus resulting in their enhanced fluorescence. In contrast, the fluorescence quenching occurred due to the electron transferring from the rich-electrons of CDs to p -benzoquinone, when p -benzenediol was oxidized to be p -benzoquinone. Therefore, a differentiating strategy of two isomers has been proposed, and also contributed to detecting m -benzenediol and p -benzenediol concentrations in the ranges of 2 × 10 −3 to 1 × 10 −7 M and 2 × 10 -5 to 2 × 10 -9 M, respectively. Although there already existed the high performance liquid chromatography and other chemical methods for determining benzenediols, the current strategy provided a new way of distinguishing and assaying the isomers of benzenediols.
研究领域:
化学
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材料科学
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分析化学
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DOI
高影响因子
Nanomaterials
·
2021
·Vol. 12
·期 1
·128
·
IF 5.076
Lei Jia,Zhitao Xu,Rujie Chen,Xiangzhen Chen,Jun Xu
Tetracycline (TC) and oxytetracycline (OTC) are the most widely used broad-spectrum antimicrobial agents in tetracycline drugs, and their structures and properties are very similar, so it is a great challenge to distinguish and detect these two antibiotics with a single probe at the same time. Herein, a dual-channel fluorescence probe (SiCDs@mMIPs-cit-Eu) was developed by integrating two independent reaction sites with SiCDs-doped mesoporous silica molecular imprinting group and europium complex group into a nanomaterial. The synergistic influence of inner filter effect and “antenna effect” can be guaranteed to solve the distinction between TC and OTC. Moreover, this novel strategy can also sequentially detect TC and OTC in buffer solution and real samples with high sensitivity and selectivity. This method revealed good responses to TC and OTC ranging from 0 to 5.5 μM with a detection limit of 5 and 16 nM, respectively. Combined with the smartphone color-scanning application, the portable and cheap paper-based sensor was designed to realize the multi-color visual on-site detection of TC and OTC. In addition, the logic gate device was constructed according to the fluorescence color change of the probe for TC and OTC, which provided the application possibility for the intelligent detection of the probe.Keywords:molecularly imprinted route;rare earth;carbon dots;distinguishing detection;tetracyclines
研究领域:
分析化学
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环境科学
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生物医学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Chaoying Tong,Li Fan,Guihan Cai,Shuyun Shi,Yangyu Yang,Ying Guo
Ammonia (NH 3 ) and hydrazine (N 2 H 4 ) present potential risks to human health, food and environmental safety. A sustainable flavonol-based probe, quercetin pentaacetate (QPA, weak blue emission 417 nm), was fabricated for dual-ratiometric fluorescent sensing and visual differentiating NH 3 and N 2 H 4 . Excited state intramolecular proton transfer-on products with green (487 nm) and yellow (543 nm) emissions occurred as meeting with NH 3 and N 2 H 4 , respectively, for their different nucleophilicities. Such a promising response offered a great opportunity of QPA to discriminatively detect NH 3 and N 2 H 4 with large Stokes shifts (>122 nm), high sensitivity (limit of detection: 35.4 μM and 0.70 ppm for NH 3 solution and gas; 0.26 μM for N 2 H 4 solution), excellent accuracy (spiked recoveries from 98.6 % to 105 %), and superior selectivity. Importantly, QPA was utilized for monitoring NH 3 vapor in fish spoilage procedures and detecting N 2 H 4 in water samples for food and environmental safety evaluation.
研究领域:
分析化学
·
环境科学
·
食品安全
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2024
·
IF 5.6
Mengmeng Ji,Yi Chen,Yufei Hu,Gongke Li
The origin of coffee beans impacts the aroma, flavor, and consumer acceptance, resulting from volatile organic compounds (VOCs) such as ketones, aldehydes, alcohols, pyrazine, and esters. Among them, ketones are associated with the refreshing fruity flavor of coffee beans and can serve as key substances for identifying their origin. Herein, the novel method of cyclic cataluminescence (CCTL) was presented and proven to be feasible for differentiating the origins of coffee beans based on the ketones-involved multiple cataluminescence reactions. A series of exponential decay CCTL peaks were measured through nano Al 2 O 3 -catalyzed reactions. By fitting the variation of peak intensity I n with time t , the characteristic attenuation coefficient k was calculated, which can be used to successfully distinguish the origin of the selected coffee beans in combination with chemometrics. The method was validated in the tests of 82 coffee beans. Coffee from the same origin has similar k values, while coffee from different origins has different k values. The discrimination accuracy of linear discriminant analysis and hierarchical clustering analysis was 100 %. The established method was fast. We anticipate that the technique could also serve as a promising new tool for reliably, effectively, and swiftly identifying the origins of other plant products.
研究领域:
食品科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF PROTEOME RESEARCH
·
2024
·
IF 3.8
Yuqing Zhang,Hui Zhao,Jinhui Zhao,Wangjie Lv,Xueni Jia,Xin Lu,Xinjie Zhao,Guowang Xu
The co-occurrence of multiple chronic metabolic diseases is highly prevalent, posing a huge health threat. Clarifying the metabolic associations between them, as well as identifying metabolites which allow discrimination between diseases, will provide new biological insights into their co-occurrence. Herein, we utilized targeted serum metabolomics and lipidomics covering over 700 metabolites to characterize metabolic alterations and associations related to seven chronic metabolic diseases (obesity, hypertension, hyperuricemia, hyperglycemia, hypercholesterolemia, hypertriglyceridemia, fatty liver) from 1626 participants. We identified 454 metabolites were shared among at least two chronic metabolic diseases, accounting for 73.3% of all 619 significant metabolite–disease associations. We found amino acids, lactic acid, 2-hydroxybutyric acid, triacylglycerols (TGs), and diacylglycerols (DGs) showed connectivity across multiple chronic metabolic diseases. Many carnitines were specifically associated with hyperuricemia. The hypercholesterolemia group showed obvious lipid metabolism disorder. Using logistic regression models, we further identified distinguished metabolites of seven chronic metabolic diseases, which exhibited satisfactory area under curve (AUC) values ranging from 0.848 to 1 in discovery and validation sets. Overall, quantitative metabolome and lipidome data sets revealed widespread and interconnected metabolic disorders among seven chronic metabolic diseases. The distinguished metabolites are useful for diagnosing chronic metabolic diseases and provide a reference value for further clinical intervention and management based on metabolomics strategy.
研究领域:
医学
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生物化学
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代谢研究
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Yiyi Liu,Fuxing Xu,Fangling Wu,Huanhuan Wang,Zhigang Liang,Chuan-Fan Ding
The distinction of enantiomers is increasingly critical in scientific research and chemical industries, because of their differences in the molecular structure and properties. R/S-Phenyl-1,2-ethanediol (PED), R,R/S,S-Hydrobenzoin (HB), and R/S-1,1,2-Triphenyl-1,2-ethanediol (TPED) are representative aromatic alcohol catalyst-class enantiomers, which directly affect the type and yield of reactants, that their chiral distinction and separation are important. In this work, the three groups of the enantiomers are recognized by measuring the ion mobility spectrometry (IMS) difference of their ternary complexes, which formed by simple non-covalent interaction with chiral selectors and metal ions. Their ion mobility was detected using trapped ion mobility spectrometry-mass spectrometry (TIMS-MS). Different chiral selectors include α-, β-, γ-Cyclodextrins (CDs), rifamycin (Rif), and natamycin (Nat) etc were investigated, and different metal ions were studied as ligands. Interestingly, the target enantiomers could not interact with the chiral selectors directly, but with the addition of metal ions, the ternary complexes of enantiomers-chiral selectors-metal ions can be formed. The mobility results reveal that the peak to peak resolution (R p-p ) was increased with the number of their benzene rings goes, which can up to 2.08. Simply, R/S-PED enantiomers can be successfully distinguished by measuring the IMS of the formed ternary complexes of [β-CD + PED + Cr-2H] + ; R,R/S,S-HB enantiomers can be distinguished by measuring the IMS of [Rif + HB + Ba-H] + and [Rif + HB + Ca-H] + ; And R/S-TPED enantiomers can be distinguished by measuring the IMS of [Nat + TPED + Mn-H] + , [Nat + TPED + Co-H] + , [Nat + TPED + Mg-H] + , [Nat + TPED + Ca-H] + , [Nat + TPED + Ba-H] + and [α-CD + TPED + Al-2H] + , respectively. Moreover, the tandem mass spectrometry experimental results showed that the binding stability of the R- and S-enantiomer ternary complexes has obvious difference and is related to different metal ion, indicating the ternary enantiomer complexes have different rigid structures. In addition, the relative quantification of R/S-enantiomer complexes were performed, good linear coefficient (R 2 ) greater than 0.99 was obtained. The method based on IMS analysis can represent an effective approach for the distinction of phenyl-Substituted ethanediol enantiomers, and the CDs, Rif, Nat can be promising chiral selectors for enantiomers distinction.
研究领域:
分析化学
·
药物化学
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DOI
高影响因子
Advanced Science
·
2024
·
IF 14.3
Hao Zhao,Yuan Liu,Gaosheng Li,Da Lei,Yuwan Du,Yudong Li,Hui Tang,Xincun Dou
Precise and timely recognition of hazardous chemical substances is of great significance for safeguarding human health, ecological environment, public security, etc., especially crucial for adopting appropriate disposition measures. Up to now, there remains a practical challenge to sensitively detect and differentiate organic amines with similar chemical structures with intuitive analysis outcomes. Here, a unique optical probe with two electrophilic recognition sites for rapid and ultra-sensitive ratiometric fluorescence detection of ethylenediamine (EDA) is presented, while producing distinct fluorescence signals to its structural analog. The probe exhibits ppb/nmol level sensitivity to liquidous and gaseous EDA, specific recognition toward EDA without disturbance to up to 28 potential interferents, as well as rapid fluorescence response within 0.2 s. By further combining the portable sensing chip with the convolutional algorithm endowed with image processing, this work cracked the problem of precisely discriminating the target and non-targets at extremely low concentrations.
研究领域:
分析化学
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环境科学
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2025
·
IF 6.1
Shuyun Zhu,Kunming Sun,Shuyi Liu,Qian Wang,Jiatong Fan,Xian-En Zhao,Guobao Xu
Flavonoid glycosides are formed by dehydration condensation of aglycones and sugar molecules. Therefore, discrimination of flavonoid glycosides from their corresponding aglycones is a challenging task because they contain the same aglycone part in their molecular structures. Herein, boric acid-functional Eu(III)-organic framework (BA-Eu-MOF) was applied to discriminate flavonoid glycosides including baicalin (Bai), wogonoside (Wog), rutin (Rut), puerarin (Pue), quercitrin (Que) and astragalin (Ast) from their corresponding aglycones for the first time. Besides as organic ligand to sensitize the luminescence of Eu 3+ through “antenna” effect, 5-boronobenzene-1,3 dicarboxylic acid provided recognition site for flavonoid glycosides. Infrared, fluorescence, UV–vis, and mass spectra were used to investigate the recognition reaction between BA-Eu-MOF and flavonoid glycosides. The data indicated that the cis -diols of flavonoid glycosides from sugars covalently bonded to boric acid group to form cyclic boronic esters, which quenched the fluorescence of BA-Eu-MOF at 620 nm through decreasing the intersystem efficiency, inner filter effect and photoelectron transfer. In contrast, aglycones could not alter the fluorescence of BA-Eu-MOF because of no covalent bond between them. This probe exhibited high sensitivity towards flavonoid glycosides with the low detection limits of 3.3 nM, 3.5 nM, 33 nM, 56 nM, 5.1 nM and 5.5 nM for Bai, Que, Wog, Ast, Pue and Rut, respectively. The unique recognition ability of boric acid group enables selective and sensitive detection of flavonoid glycosides without the interference of their corresponding aglycones.
研究领域:
化学
·
药理学
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产品关联
DOI
PubMed
高影响因子
DYES AND PIGMENTS
·
2020
·
IF 4.613
Xiuling Ji,Xiaotian Yuan,Hongjuan Nian,Pengfei Song,Yingying Xiang,Yunlin Wei,Suping Wang,Kunhao Qin,Qi Zhang,Yujiao Tu
Novel blue fluorescent carbon dots (CDs- CP ) deriving from yeast Cryptococcus podzolicus 5–2 were successfully prepared via a facile and green hydrothermal synthesis method. It exhibited uniform particle size and possessed good excitation-dependent characteristic. The dead bacterial cells were selectively labled by the CDs- CP , but the live ones not, indicating good potential for the live/dead bacterial differentiation. It was found that the prepared CDs- CP showed low biotoxicity and excellent biocompatibility with multicolour fluorescence emission properties, which provided a new insight on the bioimaging application. In addition, the prepared CDs- CP exhibits high selectivity and sensitivity in Ag(I), 2,4-dinitrophenol (2,4-DNP) and 4-nitrophenol (4-NP) detection with excellent linear ranges of 0–15 μM (R 2 = 0.9945), 0–30 μM (R 2 = 0.9959) and 0–37.5 μM (R 2 = 0.9982), respectively. The low detection limits of Ag(I), 2,4-DNP and 4-NP were 113.57 nM, 73.36 nM and 86.47 nM, respectively. It revealed that static quenching mechanism dominated in Ag(I) quenching of the CDs- CP fluorescent, while those of 2,4-DNP and 4-NP were inner filter effect. The fluorescent CDs- CP was successfully applied to determine the Ag(I), 2,4-DNP and 4-NP in real water samples with satisfactory results. Therefore, it is a good candidate for bioimaging application and environmental pollutant detection.
研究领域:
生物医学
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环境科学
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分析化学
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Wenfen Zhang,Yuancheng Wang,Qidong Zhang,Wu Fan,Guobi Chai,Lulu Guo,Qingzhao shi,Jian Mao,Changlian Zhu,Shusheng Zhang,Jianping Xie
Nicotine, a major component of tobacco smoke, exerts complex effects on the brain, including dependence and potential neurotoxicity. Understanding its distribution and metabolism in different brain regions is crucial for elucidating its neurological impact. This study presents a new solid-phase microextraction (SPME) method using a nitrogen-rich porous polymer coating (BDTB-MBD) that simultaneously quantified nicotine and its six metabolites in different rat brain regions. The coating material exhibited excellent stability with a high surface area (1001.7 m 2 /g), suitable pore diameter (5.28 nm), and excellent hydrophilic and hydrophobic properties. Under optimal conditions, the SPME tandem ultra-high-performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-MS/MS) method exhibited a wide linear range (0.01–100 μg L −1 ), low limits of detection ranging from 0.002–0.020 μg L −1 , limits of quantifications ranging from 0.006–0.070 μg L −1 , and satisfactory repeatability and reproducibility. The spiked recovery rates ranged from 77.1 % to 99.6 %, indicating that the established method was sensitive, accurate, and reliable. Density functional theory calculations revealed strong non-covalent interactions between BDTB-MBD and analytes, providing insights into the adsorption mechanism. The SPME-UPLC-MS/MS method successfully tracked the pharmacokinetics of nicotine in different rat brain tissues following intraperitoneal injection. The results demonstrated rapid absorption with a regional distribution of nicotine, followed by its gradual metabolism into cotinine, nornicotine, and other metabolites. Notably, the concentration profiles varied across brain regions, highlighting the importance of considering regional specificity in nicotine brain research. Compared with traditional techniques such as solid phase extraction, this approach offers a valuable tool for studying nicotine distribution and metabolism in the brain, with potential applications in neuroscience and drug monitoring.
研究领域:
神经科学
·
药物分析
·
毒理学
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产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Quan Wang,Xiaoyu Wang,Hui Wei
Phenol and its derivatives, known as persistent organic pollutants, have long threatened human health and environmental safety. There is an urgent need to develop convenient, low-cost, and multiplex analytical methods. Since phenols are substrates of laccase, they can be detected via laccase-catalyzed colorimetric assays. Nevertheless, the laccase-based assays cannot distinguish different phenols. Moreover, natural laccases suffer from high cost and low stability issues. To meet these needs, here we developed a laccase-like nanozyme sensor array for phenol detection and differentiation, which takes advantage of both nanozymes and cross-reactive sensor arrays. First, we examined a series of spinel-type transition metal oxides and found that manganese on octahedral sites profoundly affects the laccase-like activity of the materials. Based on the developed manganese-based spinel oxides (i.e., Mn3O4, Zn0.4Li0.6Mn2O4, and LiMn2O4), a colorimetric sensor array was constructed. The sensor array could effectively identify and discriminate phenol and its derivatives and showed good performance in the identification and differentiation of phenols in tap water samples. This work provides an important guidance for the development of laccase-like nanozymes and a promising methodology for pollutant monitoring.
研究领域:
环境科学
·
生物化学
·
纳米技术
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2023
·Vol. 15
·期 43
·5803-5812
·
IF 3.1
Fenying Wang,Shuqing Xiong,Tingting Wang,Yadan Hou,Qianjin Li
Fluorescent boronate affinity molecules have gained increasing attention in the field of fluorescence sensing and detection due to their selective recognition capability towards cis-diol-containing molecules (cis-diols). However, the conventional fluorescent boronate affinity molecules face a challenge in differentiating the type of cis-diol only by their fluorescence responses. In this study, a simple method was used to discriminate different types of cis-diols, including nucleosides, nucleotides, sugars, and glycoproteins based on the phenylboronic acid-functionalized fluorescent molecules combined with principal component analysis (PCA). Both fluorescent molecules were simply synthesized by the covalent interaction between the amino group in 3-aminophenyl boronic acid and the isothiocyanate group in fluorescein or rhodamine B. In view of their fluorescence-responsive behaviors to these cis-diols directly, it is impossible to differentiate their types even under the optimized experimental conditions. When PCA was employed to treat the fluorescence response data and the quenching constants with their molecular weight, different types of cis-diols can be distinguished successfully. As a result, by integrating the fluorescence response of the boronate affinity probes with PCA, it can greatly improve the specific recognition capability of the boronic acids, providing a simple and direct way to distinguish and identify different types of cis-diols.
研究领域:
生物化学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）