JOURNAL OF MOLECULAR LIQUIDS
·
2019
·
IF 4.561
Lele Zhou,Qi Kang,Ming Fang,Li Yu
Carboxylesterases (CES) has been regarded as a most effective biomarker candidate of the hepatoma cells and fruitfully used in the early detection. For this reason, establishing a reliable and simple system to detect CES has become attractive in the practical applications of medical diagnosis and treatment. In this study, a novel strategy for monitoring CES based on the aqueous/liquid crystals (LCs) interface was reported. A cleavable surfactant, N- octadecyloxycarbonylmethyl -N, N, N- trimethylammonium bromide (OTB) was synthesized and utilized to control the orientation of 4-cyano-4′-pentylbiphenyl (5CB) molecules on aqueous/LCs interface. The formation of OTB self-assembly monolayer promotes the alignment of the LCs, on average, normal to the surface (homotropic), whereas a corresponding black image was observed by polarized optical microscopy (POM). Subsequently, the doping of CES induces the hydrolysis of the OTB, resulting in the disruption of OTB monolayer and thereby an obvious dark-to-bright transition in the optical response. This optical transition provides a quick and easy way of detecting CES. OTB-decorated LC surfaces have been developed as a sensor, with a detection limit of 18 U/L for CES detection. As-made LCs-based sensor exhibits superior detection sensitivity and specificity towards the detection of CES, as well as the excellent long-term stability. The preliminary results shows success in sensing CES from the urine samples, which also suggests the promising detection performance of LCs sensor in practical situations. In summary, the as-prepared CES sensor, offers several advantages along with its cost-effective and easy fabrication procedure, demonstrates its potential for medically assisted therapy in human samples.
研究领域:
生物医学工程
·
医学诊断
·
传感器技术
·
分子生物学
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产品关联
DOI
高影响因子
Gels
·
2025
·Vol. 11
·期 8
·632
·
IF 5.3
Xin Zhang,Jiarong Liang,Binglei Zheng,Pengfei Jiao,Qian Xu
Elevated levels of homocysteine (Hcy) are associated with various pathological conditions including atherosclerosis, hypertension, and cardiovascular diseases. In this work, quantum-dot-based molecularly imprinted hydrogels (QD@MIHs) were developed by integrating L-cysteine-modified ZnS quantum dots (QDs)with highly selective molecular imprinting technology for rapid homocysteine detection. The QD@MIPHs were fabricated using a dual-functional monomer system (acrylamide and methacrylic acid) through surface coating of the Hcy molecularly imprinted polymer gel onto the QDs. Under optimal conditions, the response time of the QD@MIPHs for Hcy detection was 5 min. When the Hcy concentration ranged from 0.1 to 10.0 μM, the fluorescence quenching of the QD@MIHs showed a good linear relationship with Hcy concentration (R2= 0.9972), with a corresponding detection limit of 0.027 μM. In addition, the constructed QD@MIPHs showed no significant response to other interfering substances, demonstrating the high selectivity of the prepared material. Practical sample analysis revealed that the recovery rates of Hcy ranged from 94.34% to 104.1%, with relative standard deviations (RSD,n= 3) between 3.56% and 7.17%. This study provides a novel tool and method for rapid Hcy detection with significant potential in biomedical diagnostics and preventive-healthcare applications.
研究领域:
生物医学工程
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分析化学
·
纳米技术
·
生物传感器
·
高分子科学
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2018
·
IF 4.244
Jiying Xu,Yi Chen
The signal amplification methods of surface plasmon resonance (SPR) are mainly with sample selectivity . Herein proposed is a liquid core coupling SPR sensing method with adjustable sensitivity and universal applicability. A liquid core coupling unit and based on which a wavelength interrogation SPR sensor have been designed and constructed, in which a conventional chip could be used with an ingenious design and the sensitivity can be adjusted by changing the liquid core refractive index . The experimental data revealed that the sensitivity can be enhanced for 7.8 times by decreasing the liquid core refractive index from 1.5174 to 1.4502. The real applicability was validated by determination of carcinoembryonic antigen (CEA) with the signal intensity of the early stage colon cancer patient clearly up-regulated for nearly 18 folds compared with the healthy. It exhibited an adequate sensitivity, and further considering its simplicity avoiding the troublesome analysis process and/or chip preparing procedure, and extendibility to SPR imaging, the proposed method would be promising for medical diagnostics, food safety, environmental monitoring, and so on.
研究领域:
光学
·
生物传感
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医学诊断
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Xiao Wu,Daobin Jin,Ming Li,Xiliang Luo,Lei Wang
The construction of reliable and sensitive wearable sensors capable of monitoring specific targets in sweat remains a challenge highly relevant to a range of sensor/diagnostic applications. Herein, we develop a wearable microfluidic platform to monitor sweat lactate, in which spherical colloidal Photonic Crystal Hydrogel (PCH) is synthesized to construct the sensing part. Thanks to the structural color of PCH, lactate concentrations can be obtained via the eye-recognizable and low angle-dependent color variation. Accurate quantitative analysis is performed on a smart phone with Matlab code by integrating the linear relationship between hue value of sample photos and lactate concentrations. The developed sweat sensor presents good selectivity and long-term stability, and it is capable of detecting lactate accurately in human sweat within the physiological range. On-body measurement performed on volunteers successfully demonstrates the applicability of the developed device. This wearable analytical platform provides a promising tool for sweat lactate detection in sports science and medical diagnosis. And it is expected to contribute to developing user-friendly wearable biochemical sensor devices.
研究领域:
生物医学工程
·
传感器与诊断
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可穿戴技术
·
运动科学
·
医学诊断
✓ 已验证
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Taiyi Chen,Xiaoping Lin,Peisong Li,Wei Liu,Lu Jiang,Leyuan Huang,Zhongliang Li,Ruirui Zhu,Zhi-Liang Chen,Rongying Zeng,Mengqin Liu,Wen Chen
The precise detection of metal ions is crucial in food safety, biomedical diagnostics, and environmental monitoring. Although traditional “on-off” fluorescence probes are frequently utilized, their anti-interference capability remains a limitation. This study synthesized three new copper-based fluorescent probes: PTA, PTP, and PTD. Among them, PTD exhibited unique aggregation-induced emission (AIE) properties. PTD's remarkable selectivity for Zn 2+ was due to the formation of Zn 2+ -induced J-aggregates, which resulted in a concentration-dependent fluorescence redshift from green to yellow and then to red. Additionally, PTD demonstrated a rapid and sensitive response to Cu 2+ , and it was successfully integrated into a smartphone-based platform for the semi-quantitative detection of Zn 2+ and Cu 2+ in real food samples. The effectiveness in biological applications was also validated through live-cell imaging tests. This work contributes to the creation of highly selective and interference-resistant ratiometric fluorescence sensors by leveraging the special process of target metal ion-induced J-aggregate production in AIE-based probes.
研究领域:
分析化学
·
材料科学
·
生物化学
·
环境科学
·
食品科学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Zhi Luo,Yusheng Wu,Dongmiao Qin,Shuo Meng,Chujie Zeng,Biyang Deng
A luminescent reagent, 3,4,9,10-perylenetetracarboxylic acid (PTCA), was linked to a metal–organic framework (MOF) Fe(III)-MIL-88B-NH 2 through an amide reaction to form a [email protected] (III)-MIL-88B-NH 2 complex, and then gold nanoparticles (AuNPs) were grown in situ. Thus, the [email protected] (III)-MIL-88B-NH 2 @Au electrochemiluminescence (ECL) probe was obtained. The MOF of 2-aminoterephthalic acid with large specific surface area, pore sizes, and structures, which served both as the carrier and a co-reaction promoter, promoted the conversion of more S 2 O 8 2- into SO 4 •- , further enhancing the ECL signal of PTCA. An electrochemiluminescence resonance energy transfer (ECL-RET) platform was designed for the ultrasensitive and highly selective detection of cystatin C (CysC). The developed ECL-RET biosensor presented a linear range from 0.01 pg/mL to 100 ng/mL and a detection limit of 2.2 fg/mL (S/N = 3). Moreover, all the results showed that the developed ECL-RET biosensor broadened the application in the fields of biosensing and medical diagnosis.
研究领域:
分析化学
·
纳米技术
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生物医学工程
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Materials Chemistry C
·
2024
·
IF 5.7
Xizhe Li,Pengxin Xue,Haoyu Wang,Haojie Li,Ruolan Du,Jie Gao,Kwok-Yin Wong,Yong Qin
The early detection of bacterial species plays a crucial role in patient prognosis and in the development of effective therapy regimens. This study introduces an accessible and promising fluorescent sensor array designed for the classification of Gram-positive (G+) and Gram-negative (G–) bacterial species. The classification relies on 12 functionalized gold nanoclusters (AuNCs) exhibiting diverse hydrophobic and hydrophilic properties serving as sensing elements. Using these specific sensing elements, we have successfully differentiated between G– and G+ bacterial species and discriminated individual bacterial strains in various media, including water, serum, and urine, with remarkable reproducibility and high sensitivity. The excellent accuracy of the detection of unknown samples further validates the practical applicability of this sensor array. Moreover, the versatility of the sensor array is underscored by its ability to identify bacterial mixtures and bacteria with varying concentrations. In summary, this fluorescent AuNC-based sensor array offers a potent tool for bacterial analysis with promising applications in the field of biomedical diagnostics.
研究领域:
生物技术
·
医学诊断
·
纳米技术
·
微生物学
✓ 已验证
DOI
高影响因子
Materials Advances
·
2023
·Vol. 4
·期 7
·1687-1693
·
IF 5
Wenyu Wei,Manman He,Jianrui Ma,Yirui Fan,Peng Liu,Jianxi Xiao
Protein–inorganic hybrid nanomaterials are a new type of functional materials that combine the advantages of inorganic and organic polymers and nanomaterials. We herein report a simple one-pot method for synthesizing organic–inorganic hybrid nanoflowers under mild biomineralization conditions using proteins as an organic component and lanthanide fluorides as an inorganic component. The as-prepared protein–rare earth fluoride nanoflowers exhibit high performance in maintaining enzyme stability and catalytic activity due to their high surface area and graded nanostructures, and have finely tunable luminescence properties of lanthanide ions. Various proteins such as trypsin, BSA, collagen and FtsZ have all displayed excellent capability to produce Na5Yb9F32 nanoflowers, demonstrating that protein-templated biomineralization provides a universal and robust strategy for the synthesis of protein–rare earth fluoride hybrid nanoflowers. The novel luminescent protein–rare earth fluoride nanoflowers may have promising applications in biosensing, biocatalysis and medical diagnostics and treatment.
研究领域:
材料科学
·
纳米技术
·
生物化学
·
生物医学工程
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Morui Wang,Haoxuan Diao,Cheng Dou,Zhenye Wu,Liguang Ruan,Zhixin Wang,Zhong Wang,Weichun Ye,Jiangong Duan,Yuee Li
This work aims to face the challenge of label-free and quantitative detection for pathogenic microorganisms concerning on food safety and medical diagnosis. Ag NPs@PDMS sponge SERS substrates were developed through the "mold transfer-surface embedding" method for pathogenic microbial detection. The pore structure provided large specific surface area and critical contact sites, which endowed the detection limit of only 1 CFU/mL for E.coli . Importantly, six different microorganisms could be identified using this SERS substrate. The classification models combining three types of data dimension reductions and five types of machine learning algorithms were constructed to achieve the automatic classification of microorganisms. Among these algorithms, the PCA-SVC model held the highest accuracy of 92.17%. Different from labeled SERS, label-free SERS technique is conducive to the discovery of unknown microorganisms in water-environment. Therefore, this SERS substrate is expected for microbial system assessment and microbial safety monitoring, combined with handheld Raman spectrometer and artificial intelligence.
研究领域:
分析化学
·
微生物学
·
生物医学工程
·
机器学习
·
食品安全
·
医学诊断
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Haiyang Yu,Zheng Zhao,Bichen Xiao,Minghua Deng,Zhaoliang Wang,Ziqin Li,Huibin Zhang,Lei Zhang,Jingwen Qian,Jinhua Li
Thrombin is an important biomarker for various diseases and biochemical reactions. Rapid and real-time detection of thrombin that quickly neutralizes in early coagulation in the body has gained significant attention for its practical applications. Solution-gated graphene transistors (SGGTs) have been widely studied due to their higher sensitivity and low-cost fabrication for chemical and biological sensing applications. In this paper, the ssDNA aptamer with 29 bases was immobilized on the surface of the gate electrode to specifically recognize thrombin. The SGGT sensor achieved high sensitivity with a limit of detection (LOD) up to fM. The LOD was attributed to the amplification function of SGGTs and the suitable aptamer choice. The ssDNA configuration folding induced by thrombin molecules and the electropositivity of thrombin molecules could arouse the same electrical response of SGGTs, helping the device obtain a high sensitivity. The channel current variation of sensors had a good linear relationship with the logarithm of thrombin concentration in the range of 1 fM to 10 nM. The fabricated device also demonstrated a short response time to thrombin molecules, and the response time to the 1 fM thrombin molecules was about 150 s. In summary, the sensing strategy of aptamer-based SGGTs with high sensitivity and high selectivity has a good prospect in medical diagnosis.
研究领域:
生物传感器
·
纳米技术
·
生物医学工程
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Wei Liu,Wenxiu Yu,Xiaogang Li,Xiaoyong Zhao,Yu Zhang,Pei Song,Yongmei Yin,Rimo Xi,Meng Meng
Here we present a fluorogenic multianalyte biosensor based on self-assembled tetraphenylethene (TPE) molecules by modification with diethylenetriamine (TPDA). The TPDA probe could specifically react with pyrophosphate (PPi) by hydrogen linkage. Simulation analysis results indicated that the coupling will lead to intermolecular cross-linking of TPDA molecules and turn on aggregated-induced emission at 470 nm. When alkaline phosphatase (ALP) was introduced, PPi was hydrolyzed and fluorescence emission was turned off. The proposed system allows sensitive detection for ALP activity (LOD = 0.09 mU mL −1 ) and PPi (LOD = 66.7 nM). For ALP assay, this is the first example using PPi to switch AIE phenomenon, which offers better pH selection (pH 7.4) than conventional methods (pH 8.0–9.6). For PPi analysis, this method does not require metal ions, indicating better stability with little interference. We further applied the TPDA-PPi complex as a new substrate for ALP in an immunoassay and proved that the substrate is more sensitive (LOD = 0.8 ng mL −1 ) than traditional one (LOD = 4.0 ng mL −1 ), which means that the complex is greatly potential in ultrasensitive immunoassay for biomedical diagnosis.
研究领域:
分析化学
·
生物化学
·
生物医学工程
·
分子生物学
✓ 已验证
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2019
·Vol. 411
·期 12
·2531-2543
·
IF 3.286
Liu Haijian,Jia Lu,Wang Yixuan,Wang Mengyao,Gao Zideng,Ren Xueqin
In this work, a novel ratiometric fluorescent sensor, based on carbon dots (CDs) and gold nanoclusters (AuNCs), is developed for highly sensitive and selective visual colorimetric detection of Cu 2+ and alkaline phosphatase (ALP). The ratiometric fluorescent sensor was synthesized by covalently linking 11-mercaptoundecanoic acid (11-MUA)–stabilized AuNCs to the surface of amino-functionalized CD/SiO 2 nanoparticles. The red fluorescence of the AuNCs can be quenched by Cu 2+ owing to coordination between Cu 2+ and 11-MUA; however, the blue emission of the CDs was insensitive to Cu 2+ owing to the protective silica shell. The quenching of the AuNCs’ fluorescence returned when PPi was added because of the higher affinity between Cu 2+ and PPi than that between Cu 2+ and 11-MUA. In the presence of ALP, PPi was catalytically hydrolyzed into phosphate (Pi), which showed a much weaker affinity for Cu 2+ . Thus, Cu 2+ ions were released, and the fluorescence of the AuNCs was quenched once more. Based on this principle, Cu 2+ and ALP could be simultaneously detected. The developed ratiometric fluorescent sensor could detect Cu 2+ over a range from 0.025 to 4 μM with a detection limit of 0.013 μM and ALP over a range from 0.12 to 15 U/L with a detection limit of 0.05 U/L. The present method was successfully applied for the detection of Cu 2+ and ALP in real water samples and in human serum samples, respectively. This ratiometric fluorescent approach may provide a highly sensitive and accurate platform for visual Cu 2+ and ALP sensing in environmental monitoring and medical diagnosis.
研究领域:
分析化学
·
环境科学
·
医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）