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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES · 2025 · IF 8.5

Enhancement of antioxidant, carbonyl scavenging and anti-glycation activities of polysaccharide-based hydrocolloids by covalent grafting with gallic acid

Mianzhang Zhang,Hongliang Li,Fu Liu,Shiyi Ou,Pengzhan Liu,Jie Zheng

Carbonyl stress contributes to pathological disorders leading to the progression of a variety of chronic diseases. Exploration of food ingredients with carbonyl scavenging capacities became one of the most potential strategies for the prevention of these diseases. Polysaccharide-based hydrocolloids have wide application approaches in the food industry. However, the carbonyl scavenging capacity of some natural polysaccharide-based hydrocolloids is unsatisfactory due to structural limitations. In this research, gallic acid (GA) was grafted onto different hydrocolloids (xanthan gum, carrageenan, carboxymethylcellulose, carboxymethyl chitosan (CMCS), and sodium alginate) by a free radical-mediated reaction to improve their antioxidant, carbonyl scavenging, and anti-glycation activities. The antioxidant activity of hydrocolloids was significantly improved after GA grafting. Among them, CMCS-GA (graft ratio 15.45 mg GA/g) exhibited the same ABTS scavenging ability as the equivalent amount of free GA and Vc. The carbonyl scavenging capacity, and the inhibitory effects on the fluorescent advanced glycation end products (AGEs) and protein oxidation products of some hydrocolloids also increased significantly GA grafting. The scavenging capacity of CMCS-GA on MGO, GO, ACR, and MDA in the model reaction increased by 220 %, 100 %, 6 %, and 58 %, respectively. Overall, CMCS-GA showed the best performance in radical scavenging, carbonyl scavenging, and AGEs inhibition compared to all the other hydrocolloids investigated. The findings showed that grafting GA onto hydrocolloids is a viable strategy to enhance the carbonyl scavenging capacity and anti-glycation activity of hydrocolloids.

研究领域: 食品科学 · 生物化学
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JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE · 2025 · IF 3.5

Study of polysaccharide–gallic acid interaction and digestion behavior in biomimetic dietary fiber

Wen Zhang,Yue Zhang,Qian-yu Zhang,Xue-ning Ren,Ji-xiang Ge

BACKGROUND Gallic acid (GA) is recognized for its significant antioxidative and anti-inflammatory properties. The processing and consumption of GA-rich foods influence the interaction with cell wall polysaccharides, thereby impacting the bioavailability and bioaccessibility of GA. This study aimed to develop a biomimetic dietary fiber (BDF) model composed of bacterial cellulose, pectin, and GA to investigate the interactions, digestive behavior, and antioxidant synergism between polysaccharides and GA within the BDF. RESULTS The microstructural, compositional, and crystalline characteristics of the synthesized BDF were found to be comparable to those of natural dietary fibers. During simulated digestion in the stomach and small intestine, a total of 38.02% of GA was released from the BDF, while 61.98% remained in a polysaccharide–GA conjugated form post-digestion. In free radical scavenging assays, the GA bound within the BDF exhibited a greater antioxidant capacity than free GA. CONCLUSION The interactions between polysaccharides and GA are mediated by hydrogen bonding, hydrophobic interactions, van der Waals forces, and other non-covalent interactions. The release mechanism of GA involves its escape and diffusion from the BDF network structure, the disintegration of the BDF, and the dissolution of pectin. Polysaccharides provide a protective effect on the bioactivity of GA and enhance its synergistic role in antioxidative stress, thereby improving its bioaccessibility. These findings lay the groundwork for further exploration of the interaction mechanisms between polysaccharides and GA in dietary fibers and hold significant implications for the development of dietary intervention strategies aimed at mitigating oxidative damage. © 2025 Society of Chemical Industry.

CAS / MDL: 1303-00-0 9000-69-5
研究领域: 食品科学 · 营养学
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