Sui Xiao,Li-Fang Zhang,Xu Zhang,Su-Mei Li,Fei-Qun Xue
Several Acalypha australis Linn. species are used in traditional medicine in Southeast Asia. In this work, the ultra-performance liquid chromatography/mass spectrometry fingerprints and the antibacterial activities of A. australis Linn. were investigated. An in-depth discussion on the reliability of identifying and obtaining potentially active compounds by spectrum-effect relationship and semi-preparative high performance liquid chromatography was conducted. The result shows that gallic acid and a compound with molecular weight of 634.1 in the fingerprints were the main antibacterial compounds. Compared to the crude extract of A. australis Linn., both compounds increase the antibacterial efficacy 10 to 20 times. Compounds with molecular weights of 154.0, 292.0, and 485.1 in the fingerprints were the auxiliary antibacterial compounds. Through the entire isolation procedure, we obtained these antibacterial compounds with purities of 92.53, 87.98, 90.73, 89.36, and 88.14%, respectively. This work provides a general model of the combination of ultra-performance liquid chromatography/mass spectrometry fingerprinting and antibacterial test to study the spectrum-effect relationships of A. australis Linn. This model can be used to discover further the active compounds of this herb.
Flos Dolichoris Lablab, as a flower of a homologous plant with medicinal and food applications, has rich plant activity with research value. In this article, we studied flavonoids in Flos Dolichoris Lablab, and an effective semipreparative HPLC separation method was established. Then, seven flavonoids from Flos Dolichoris Lablab were prepared in one step, and their antioxidant activity was evaluated. The results show for the first time that the seven flavonoids kaempferol 3-O-sophoroside (1), quercetin 3-rutinoside (2), quercetin 3-glucoside (3), kaempferol-3-O-robinobioside (4), kaempferol-3-O-rutinoside (5), isorhamnetin 3-O-neohesperidoside (6), and kaempferol-3-O-galactoside (7) were isolated from the genus Lablab. Antioxidant activity results with individual compounds show that compounds 2, 3, and 7 exhibit better antioxidant activity. Experiments on different combinations of flavonoids of Flos Dolichoris Lablab indicate that the interaction of flavonoids can affect the mixture's total antioxidant activity. This research provides guidelines for preparing flavonoids present in Flos Dolichoris Lablab and structure-activity relationship studies.
危险品化学品经营许可证（带存储）