JOURNAL OF ETHNOPHARMACOLOGY
·
2016
·
IF 3.055
Hui-hui Xie,Jian-ya Xu,Tong Xie,Xin Meng,Li-li Lin,Li-li He,Hao Wu,Jin-jun Shan,Shou-chuan Wang
Ethnopharmacological relevance Banxia (BX) is the root of Pinellia ternata (Thunb.) Berit. Its processed products, such as Jiang Banxia (JBX), have been clinically used in traditional Chinese medicine to treat vomiting, coughing , and inflammation. However, data for their safety for pregnant women are contradictory and confusing. Aim of the study To further explore the safety of BX, an ultra-performance liquid chromatography coupled with liquid chromatography–mass spectrometry (LC–MS) and gas chromatography-mass spectrometry (GC–MS) metabolomics approach was used to evaluate the metabolic perturbation in pregnant rats caused by BX and JBX. Materials and methods Placenta and amniotic fluid samples were collected from control Sprague-Dawley pregnant rats and exposed to BX suspension and JBX decoction (1.434 g/kg/day). Samples were analyzed using LC–MS and GC–MS. The acquired MS data of above samples were further subjected to multivariate data analysis, and the significantly altered metabolites were identified. The associated pathways were constructed using MetaboAnalyst 3.0. Results The weight and histopathology of the placenta from each group of rats had no definite difference. However, we found 20 differential endogenous metabolites that changed significantly in the placenta and amniotic fluid samples. The alterations of identified metabolites indicated a perturbation in glycerophospholipid metabolism, amino acid metabolism, and carbohydrate metabolism in pregnant rats exposed to BX and JBX. Conclusion In summary, this work suggested that oral administration of BX and JBX may induce disturbances in the intermediary metabolism in pregnant rats. This work contributes to further understanding the safety of BX and its processed products.
研究领域:
药理学
·
传统医学
·
代谢组学
✓ 已验证
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2022
·Vol. 414
·期 23
·6871-6880
·
IF 4.478
Qi Qiaofang,Shang Cuiling,Wang Huayu,Ge Chunpo,ZhijunYang,Ni Tianjun,Chang Kaiwen
In this work, we introduced the acrylate recognition group into dicyanoisophorone derivative DCI-C–OH to construct the NIR fluorescent probe DCI-C-Cys with a large Stokes shift (240 nm). DCI-C-Cys could specifically respond to Cys, resulting in a 22-fold increase in fluorescence intensity at 702 nm. Meanwhile, the probe has the advantages of good water solubility, high sensitivity (93 nM), and excellent biocompatibility. Moreover, DCI-C-Cys successfully monitored endogenous and exogenous Cys in HepG2 cells and zebrafish. Most importantly, we found that balsam pear polysaccharide could lead to the increase of intracellular Cys levels, which might be conducive to the further study of the antioxidant mechanism of balsam pear polysaccharide. Graphical abstract
研究领域:
生物化学
·
分子生物学
·
医学
✓ 已验证
DOI
PubMed
高影响因子
Food & Function
·
2018
·Vol. 9
·期 11
·5880-5890
·
IF 3.289
Xiuling Tang,Huanhuan Zhao,Wei Jiang,Song Zhang,Shun Guo,Xiaobo Gao,Peng Yang,Lei Shi,Linna Liu
Dry citrus peel (Chenpi) is not only consumed as a dietary supplement, but also used for the treatment of respiratory diseases. Pinelliae Rhizoma Praeparatum (Banxia) is always used with Chenpi for the treatment of respiratory diseases, but β-sitosterol, the main active component in Banxia, as a food additive, shows no respiratory system activity. In the present study, the pharmacokinetic characters showed that the absorption of the active components of Chenpi was accelerated under pathological conditions combined with Banxia. Although the bioavailability of active components did not significantly change, the distribution of active components in tissues increased, particularly in the target organ. These results are consistent with the combination of Chenpi with β-sitosterol. Furthermore, the pharmacodynamics result also indicated that Chenpi combined with Banxia or β-sitosterol was able to ameliorate histopathologic damage and decrease the levels of inflammatory factors. The results suggest that pharmacokinetic interactions improve the pharmacological activity of Chenpi in respiratory diseases.
研究领域:
药理学
·
中药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
·
2024
·
IF 4.8
Lang Ren,Xingqiu Ruan,Hanlin Dong,Yiyao Cheng,Kinyu Shon,Cheng Chang,Renjun Gu,Zhiguang Sun
Ethnopharmacological relevance Banxia Xiexin Decoction (BXD) is a traditional herbal formulation with a bitter flavor that has a long-standing history of use in Asia for treating functional dyspepsia (FD). In traditional Chinese medicine, the bitter flavor is believed to play a critical role in the therapeutic activity of BXD. The ethnopharmacological properties of bitter plant extracts are closely associated with their anti-inflammatory effects, which may contribute to their efficacy in FD. However, the specific mechanisms remain unknown. Aim of the study The objective of this study is to uncover the bitter active compounds of BXD and their effects in the treatment of FD. Materials and methods The chemical compounds of BXD were identified using HPLC-Q-Exactive-MS. Active compounds in BXD were obtained from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP) database, and bitter active compounds were further identified using BitterDB and PlantMolecularTasteDB. Molecular docking was employed to identify potential targets of these bitter active compounds, and their activation was validated through flow cytometry analysis of Ca 2+ . Subsequently, a mouse model of FD was established, and our hypothesis was further validated using enzyme linked immunosorbent assay, immunohistochemistry, immunofluorescence, and western blotting. Results Through HPLC-Q-Exactive-MS analysis, TCMSP, BitterDB, and PlantMolecularTasteDB database, a total of 11 bitter active compounds in BXD were identified: Baicalein, Baicalin, Berberine, Coptisine, Formononetin, Isorhamnetin, Kaempferol, Naringenin, Palmatine, Quercetin, and Wogonin. Molecular docking results indicated that these active compounds exhibited strong affinity for TAS2R38, with Berberine showing the highest scoring. Flow cytometry analysis of Ca 2+ revealed that both Berberine and BXD elevated intracellular calcium concentrations, although this effect was partially antagonized by the TAS2R38 inhibitor probenecid. In vivo experiments demonstrated that BXD effectively improved eosinophil infiltration in the duodenum of FD mice, downregulated the expression of inflammatory factors IL-1β, IL-5, and TNF-α, inhibition of NF-κB signaling pathway activation, alleviated damage to the duodenal mucosal barrier, and reversed gastrointestinal motility disorders, with the therapeutic effect enhancing with increasing doses of BXD. However, this therapeutic effect was partially inhibited following probenecid intervention. Conclusion BXD contains numerous bitter active compounds that play a significant role in regulating inflammatory activity in the duodenum of FD through the activation of TAS2R38. This finding unveils, for the first time, the ethnopharmacological activity of bitter plant taste agents in anti-inflammatory effects, providing new insights for the treatment and drug development of FD.
研究领域:
民族药理学
·
分子生物学
·
中药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Radiation Research and Applied Sciences
·
2022
·
IF 1.773
Lifen Meng,Zhi Xia,Jingsong Lv,Gaofu Liu,Ye Tan,Qianzhu Li
In this paper, five phenolic acids in the rhizosphere soil of Pinellia ternate were determined to have strong allelopathy to Pinellia ternate . The phenolic acids in rhizosphere soil and non continuous cropping soil of Pinellia ternate for two years were extracted and detected by GC-MS, 5 phenolic acids were found to have different content changes. By comparing the contents of phenolic acids in the rhizosphere soil before, during and after planting, and combining with the literatures, it was determined that protocatechuic acid, vanillic acid, vanillin, syringate and syringaldehyde had strong allelopathy on the growth and development of Pinellia ternate . Furthermore, the effects of the above five phenolic acids on the growth of Pinellia ternate , the growth of seedlings and the comparison of tuber size were investigated. The results showed that the five phenolic acids had inhibitory effects on the growth of Pinellia ternate .
研究领域:
植物学
·
生态学
·
农学
✓ 已验证
产品关联
DOI
高影响因子
World Journal of Clinical Oncology
·
2024
·Vol. 15
·期 10
·1293
·
IF 2.6
Guo-Xiu Zu,Ke-Yun Sun,Xi-Jian Liu,Ji-Qin Tang,Hai-Liang Huang,Tao Han
BACKGROUND In China banxia xiexin decoction (BXD) has been used in treating gastric cancer (GC) for thousands of years and BXD has a good role in reversing GC histopathology, but its chemical composition and action mechanism are still unknown. AIM To investigate the mechanism of action of BXD against GC based on transcriptomics, network pharmacology, in vivo and in vitro experiments. METHODS The transplanted tumor model was prepared, and the nude mouse were pathologically examined after administration, and hematoxylin-eosin staining was performed. The active ingredients of BXD were quality controlled and identified using ultra-performance liquid chromatography tandem quadrupole electrostatic field orbitrap mass spectrometry (UPLC-Q-Orbitrap MS/MS), and traditional Chinese medicines systems pharmacology platform, drug bank and the Swiss target prediction platform to predict the relevant targets, the differentially expressed genes (DEGs) of GC were screened by RNA-seq sequencing, and the overlapping targets were analyzed to obtain the key targets and pathways. Cell Counting Kit-8, apoptosis assay, cell migration and Realtime fluorescence quantitative polymerase chain reaction were used for in vitro experiments. RESULTS All dosing groups inhibited the growth of transplanted tumors in laboratory-bred strain nude, with the capecitabine group and the BXD medium-dose group being the best. A total of 29 compounds and 859 potential targets in BXD were identified by UPLC-Q-Orbitrap MS/MS and network pharmacology, RNA-seq sequencing found 4767 GC DEGs, which were combined with network pharmacology and analyzed 246 potential therapeutic targets were obtained and pathway results showed that BXD may against GC through the Phosphoinositide 3-kinase (PI3K)/protein kinase B (AKt) signaling pathway. In vitro cellular experiments confirmed that BXD-containing serum and LY294002 could inhibit the proliferation of GC cells, promote apoptosis, and inhibit the migration of GC cells by decreasing the expression of EGFR , PIK3CA , IL6 , BCL2 and AKT1 in the PI3K-Akt pathway in MGC-803 expression. CONCLUSION BXD has the effect of inhibiting tumor growth rate and delaying the development of GC. Its mechanism of action may be related to the regulation of PI3K-Akt signaling pathway.
研究领域:
肿瘤学
·
药理学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
International Journal of Electrochemical Science
·
2023
·
IF 1.5
Zhi Xia,Yu Zhang,Qianzhu Li,Haijun Du,Guofeng Gui,Gaoyu Zhao
The rapid electrochemical determination of ferulic acid (FA) was assiduously scrutinized by cyclic voltammetry (CV) using graphene oxide sheets (GOs) and multi-walled carbon nanotubes (MWCNTs) nanocomposites modified glassy carbon electrode. The synergistic out-come of GOs and MWCNTs resulted in superior electrocatalysis performance. Under optimal conditions, the peak current was proportional to FA concentration in the range of 0.24 μM to 32 μM, and 88 μM to 1.23 mM, with a relative low detection limit of 0.08 μM. Besides, it was successfully applied to detect FA in Pinellia ternate with recovery values in the range of 102.8%-113.2%, which made it a potential alternative tool for the quantitative detection of FA in pharmaceutical analysis and quality evaluation of traditional Chinese medicine.
研究领域:
药学
·
分析化学
·
传统中药研究
✓ 已验证
DOI
高影响因子
Journal of Nanoelectronics and Optoelectronics
·
2022
·Vol. 17
·期 1
·22-29
·
IF 0.697
Meng Lifen,Lv Jingsong,Li Qianzhu,Tan Ye,Xia Zhi,Wu Haizhi
In this study, trace amounts of heavy metal ions copper, lead, mercury and cobalt in Pinellia ternata samples were detected by ionic liquid ultrasonic assisted dispersion liquid–liquid microextraction, combined with high performance liquid chromatography-ultraviolet detector.
The method used sodium diethyldithiocarbamate as complexing agent and phosphoric acid ionic liquid Trihexyl(tetradecyl) phosphine bis[(2,4,4-trimethyl)] amyl phosphonate (cyclophosphate IL104) as extractant. Methanol: 0.2% phosphoric acid (60:40) was used as mobile phase for separation and
UV detector was used for detection, the flow rate was 1 mL/min and the injection volume was 10 μL. In this paper, the effects of sample pH, complexing agent concentration, ultrasonic time, centrifugation time and speed and ionic strength were optimized. Under the optimal conditions
(50 μL ionic liquid cyclic phosphine IL104, pH = 5, 0.5% sodium diethyldithiocarbamate, ultrasonic time of 30 min, centrifugation speed of 4000 r/min, 0.1 g NaCl), the recovery of spiked standard was 89.0~98.2%. High performance liquid chromatography had the characteristics of low
detection limit, accurate and reliable determination results, high sensitivity, strong specificity and good reproducibility.
研究领域:
中药分析
·
环境毒理学
✓ 已验证
DOI
高影响因子
Frontiers in Plant Science
·
2022
·
IF 6.627
Zhigui He,Yanfeng Wang,Yan Yan,Shaowei Qin,Huan He,Renjun Mao,Zongsuo Liang
Pinellia ternata (Thunb.) is a famous traditional Chinese medicine with high medicinal value, but its culture is strongly hindered by the continuous cropping obstacles (CCO) which are tightly associated with allelopathic effects. Deciphering the response mechanisms of P. ternata to allelochemicals is critical for overcoming the CCO. Here, we elucidate the response of P. ternata to phenolic acids treatment via physiological indices, cellular approaches, and transcriptome analysis. Phenolic acids showed a significant effect on the growth of P. ternata seedlings, similar to the phenotype of continuous cropping. Cellular analysis demonstrated that phenolic acids remarkably induced root cell death. Physiological analysis revealed that phenolic acids induced the overaccumulated of H2O2 and
O2−
in root cells. However, two exogenous antioxidants (L-ascorbic acid and β-gentiobiose) aid in the scavenging of over-accumulated H2O2 and
O2−
by promoting the antioxidant enzyme activity such as superoxide dismutase (SOD), ascorbate peroxidase (APX), and catalase (CAT). Transcriptome analysis demonstrated that differentially expressed genes (DEGs) related to the cell wall degeneration and reactive oxygen species (ROS) metabolism were upregulated by phenolic acid treatment. In addition, downregulated DEGs involved in sucrose and starch metabolism and phenylpropanoid biosynthesis pathways decreased the key metabolites contents. Taken together, phenolic acids caused root cell death by inducing the overaccumulation of H2O2 and
O2−
, and L-ascorbic acid and β-gentiobiose effectively alleviated ROS stress. The present study elucidates the underlying mechanism of the allelopathic effect of phenolic acids, offers valuable information for further understanding the mechanism of CCO, and could contribute to improving guidance for further P. ternata production.Keywords: Pinellia ternata, allelopathic effect, phenolic acids, antioxidant enzyme activity, reactive oxygen species, transcriptome, continuous cropping obstacles
研究领域:
植物科学
·
分子生物学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2014
·
IF 4.258
Zhenhao Li,Shun Xiao,Ni Ai,Kedi Luo,Xiaohui Fan,Yiyu Cheng
In comparison with monotherapy in western medicine, traditional Chinese medicine (TCM) advocates combinational therapy for treating diseases and TCM formula is a representative for this approach. Despite of extensive clinical applications of TCM formulae, knowledge about their pharmacological activities, mechanisms of action and cellular targets remains limited. A main contributing factor to these unanswered questions is unavailability of chemical compositions and their contents in the formulae. Several challenges hinder global qualitative and quantitative analysis of the formulae, including large quantities of constituents, potential physicochemical changes during decoction and lack of authentic standards. Herein we introduced an integrated strategy based on liquid chromatography coupled with mass spectrometry (LC-MS) systems to address such challenges. First, liquid chromatography-ion trap and quadrupole time-of-flight mass spectrometry (LC-IT/MS and LC-QTOF/MS) were utilized to characterize chemical profiling of the formulae. Meanwhile, MS 2 of IT/MS produced major parameters for derivative multiple reaction monitoring (DeMRM) on liquid chromatography-triple quadrupole mass spectrometry (LC-TQ/MS), which offered rapid and direct transition design in the quantitative assay. Instead of authentic standards, serial dilutions of single herbs were employed in this study to construct calibration curves necessary for calculating relative concentrations of components. Xiao-Banxia decoction and its four analogous formulae were then taken to exemplify the feasibility of currently proposed methodology. Among the 160 qualitatively identified components, a total of 138 components were semi-quantified for these decoctions. Based on these results, we demonstrated that co-decoction of different herbs could result in concentration variations of components and this effect was more prominent when certain herbs were combined. Our results indicated that the present strategy would significantly contribute to chemical studies on TCM and its utilities could be extended to other research fields, such as metabolomics and comparative chemistry.
研究领域:
中药学
·
分析化学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Foods
·
2024
·Vol. 13
·期 24
·4019
·
IF 4.7
Zhou Xu,Yanxia Xiong,Pei Hu,Long Chen,Jianhua Wan,Chenggang Huang,Wenjun Liu
Alpinia officinarumHance has a medicinal history of thousands of years in treating cough, diabetes, and gastrointestinal system diseases, and it is also a medicine food homology (MFH) plant in China. To evaluate the pharmacological activities of polysaccharides from the rhizomes ofA. officinarum, polysaccharides were initially obtained by hot-water extraction and the ethanol precipitation method. A homogenous polysaccharide designated as AOP-w was isolated by a DE-52 column. The proposed structure was elucidated and the immunoregulatory effects on RAW 264.7 macrophage cells were evaluated. The results showed that AOP-w had a molecular weight of 5.26 kDa, and mainly consisted of galactose and glucose (molar ratio of 0.12:0.88). Its backbone comprised α-(1→4)-Glcp, α-(1→4,6)-Glcpand β-(1→3,4)-Galpresidues, terminated by α-(1→6)-Glcpand T-Glcpresidues. AOP-w was nontoxic to RAW 264.7 cells, but demonstrated promotion in cell proliferation within a 100 μg/mL concentration. The immunostimulatory effects of AOP-w were confirmed by the elevated NO production of AOP-w-treated cells. Moreover, the RNAseq was conducted and the results showed that AOP-w may activate the TNF and NF-κB signaling pathways by binding to Toll-like receptors, thereby affecting the immune modulatory activity of RAW264.7 cells. These results suggest a high potential of AOP-w fromA. officinarumfor immunotherapeutic applications.
研究领域:
药理学
·
分子生物学
·
免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PHYTOCHEMICAL ANALYSIS
·
2019
·Vol. 31
·期 2
·242-251
·
IF 1.963
Luwei Fang,Jinfeng Xie,Liangwen Lin,Minglei Tian,Kyung Ho Row
Introduction Ephedrine is a typical compound found in lots of plant species that is used in several medicines for the treatment of asthma and bronchitis. However, excess amounts are harmful to humans, so it needs to be removed. Objective This study developed a multi-phase extraction (MPE) method with a molecular imprinted polymer (MIP) coated ionic liquid (IL)-based silica (SiO 2 @IL@MIP) to simultaneously extract and separate ephedrine from Pinellia ternata , 10 medicines, and urine samples. Methods IL was immobilized on silica. Subsequently, the IL was combined with the functional monomer, followed by the addition of the crosslinker and template. The resulting sorbent was applied to the MPE, and the extraction, washing and elution solvents were evaluated. Results Fourier-transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM) confirmed the synthesis of SiO 2 @IL@MIP. A maximum adsorption amount of 5.76 mg/g was obtained at 30°C at a neutral pH. In MPE, 10.00 mL of methanol could extract all the ephedrine from Pinellia ternata . The interference was removed by washing with 4.00 mL of water, ethanol, and acetonitrile. Finally, 8.00 mL of methanol/acetic acid (99:1, v / v ) was applied as the elution solvent. The following were extracted: 5.50 μg/g of ephedrine from Pinellia ternata , 0.00–46.50 μg/g from the 10 herbal medicines, and 68.70–102.80 μg/mL in the urine samples. Conclusion The proposed method was applied successfully to the simultaneously extraction and separation of ephedrine from plants and medicines. These results are expected to provide important data for the development of new methods for the separation and purification of bioactive compounds.
研究领域:
分析化学
·
药学
·
植物化学
·
分离科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）