JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2022
·
IF 3.571
Houwei Lin,Yinlian Yuan,Tian Hang,Peng Wang,Shijiao Lu,Hang Wang
In recent years, the development and optimization of biodegradable coronary stents have become the research focus of many medical device manufacturers and scientific research institutions since they can be completely degraded and absorbed, and they restore vascular function. However, there is a lack of in situ quantification of these stents spatially in tissue in vivo . In this study, matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT ICR) and time-of-flight (TOF) mass spectrometric imaging (MSI) were used to analyze the time-dependent distributions of a biodegradable vascular scaffold, which consisted of copolymers of lactic acid and glycolic acid (PLGA) and its degradation products in cross-sections and longitudinal sections of blood vessels. The MALDI-MSI methods for analyzing the distribution of PLGA and its derivatives in vivo were established by optimizing the conditions of sample pretreatment and mass spectrometry (MS). In order to semi-quantify the contents of PLGA degradation products in blood vessels, self-made stainless-steel and indium tin oxide (ITO) target plates were developed to compare and establish the standard curves for semi-quantitative analysis. The target plate can be placed on the target carrier of MS simultaneously with the conductive slide, which can simultaneously carry out vapor deposition or spray on the substrate, to ensure the parallelism of the pretreatment experiments between the standards and the actual vascular samples. The proposed method provided a powerful tool for evaluating the distributions and degradation process of biological stent materials in the coronary artery, as well as provided technical support for the research and development of degradable biological stents and product optimization.
研究领域:
医学影像学
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材料科学
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生物医学工程
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DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
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2017
·
IF 3.887
Li-Hong Ma,Hai-Bo Wang,Bi-Yun Fang,Fang Tan,Yuan-Cheng Cao,Yuan-Di Zhao
In this paper, visual detection of trace lead ion was established by aptamer and silver staining. The basic strategy was that aminated PS2.M aptamer was immobilized onto slide and formed stable G-quadruplex structure. PbS was generated by adding S 2− , and it catalyzed subsequent silver staining reaction, through the silver staining amplification effect, the slide presented visible ash black. The gray value of slide after silver staining was analyzed and the semi-quantitative detection of Pb 2+ in solution was realized. The results showed that optical darkness ratio (ODR) and logarithmic value of Pb 2+ concentration had a good linear relationship (R 2 = 0.951) over the range of 0.5–10 μM. In addition, there was no obvious interference of other common metal ions for the detection, indicating that this method presented outstanding selectivity. And it was also used for qualitative and semi-quantitative determination of Pb 2+ in soil sample successfully.
研究领域:
生物化学
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环境科学
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产品关联
DOI
PubMed
高影响因子
INORGANIC CHEMISTRY COMMUNICATIONS
·
2025
·
IF 5.4
Chunhui Wang,Fengshan Liu,Qiusheng Yang,Yang Xu
Developing facile analytical methods and portable instruments to quantify existence of microplastics (MPs) in environmental samples is demanding but remains challenging. In this study, a method exploiting carbon dots (CDs) as fluorescence source and XRD quartz plate as substrate was proposed for semi-quantitative MPs based on fluorescence “turn on” strategy. After excitation at 365 nm, non-fluorescence CDs film was brightened with yellow emission in the presence of polyethylene (PE) MPs due to the surface environment change of CDs. For realizing facile detection, smartphone and ImageJ software were used to capture the fluorescence signals of the CDs film. A linear curve was fitted to be y = 0.31627 x + 0.77396 with a linear range of 2–22 μg. Furthermore, the method was further applied on water samples to demonstrate its sensing capability. Finally, a portable sensor involving this analytical method was proposed. This work not only presents a facile analytical method for quantification of PE MPs, but also provides a tool to apply in the field of MPs management.
研究领域:
环境科学
·
分析化学
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产品关联
DOI
高影响因子
CHEMISTRY AND PHYSICS OF LIPIDS
·
2019
·
IF 2.536
Yuan Gao,Shimin Wu,Renhuai Cong,Junyong Xiao,Fangli Ma
Schisandra chinensis is a medicinal and edible plant that contains various bioactive compounds. Among these, lignans are the major functional compounds. Nevertheless, detailed information about lignans in Schisandra chinensis oil remains scarce. A powerful UPLC-Q/TOF-MS method was established for the rapid identification of the lignan constituents of Schisandra chinensis oils. The results showed that 21 lignans have been unambiguously identified, and four lignans have been tentatively identified in the Schisandra chinensis oils. In addition, semi-quantitative analysis indicated that the total lignan content in the Schisandra chinensis oils was distributed from 67.73 ± 0.06 to 87.61 ± 1.83 mg/g. Schisandrin and schisandrin B were the most abundant lignans in the Schisandra chinensis oils, their content ranging from 15.85 ± 0.09 to 20.57 ± 0.38 mg/g. Additionally, this study provided a systematic characterization of lignans in Schisandra chinensis oil and indicated that the oil might be used as lignan-related functional foods.
研究领域:
植物化学
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分析化学
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药理学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Ming Li,Caiping Ding,Pengde Jia,Longhua Guo,Sui Wang,Zhiyong Guo,Fengmei Su,Youju Huang
p -Aminophenol (p-AP) is one of the common environmental pollutants with teratogenic effects, nephrotoxicity and genotoxicity. In this study, a novel colorimetric method for quickly, sensitive and on-site detection of p-AP was developed based on target induced etching of AuNRs, which will generate colorful color changes. Our investigation reveals that potassium iodate (KIO 3 ) can be reduced by p-AP to generate iodine (I 2 ), and I 2 can etch CTAB stabilized AuNRs along the longitudinal direction. As an emerging fascinating colorimetric material, LSPR extinction peak of AuNRs will shift to short wave direction with a color change from the initial tea red to brown, gray, dark green, cyan, blue, indigo, purple, purplish red and final pink. Under the optimal conditions, our strategy is analytically specific and sensitive for the detection of p-AP from 0.5 μM to 20 μM by the naked eye, and a detection limit of 0.27 μM was achieved. Meanwhile, this colorimetric method is used to detect p-AP in environmental water, paracetamol tablets and human urine. Hence, this proposed strategy has the advantages of rapid semi-quantitative analysis of p-AP with naked eyes, simple operation and good application prospect.
研究领域:
环境科学
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分析化学
·
生物医学工程
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Peng Yu,Yuanming Zhai,Rui-Ying Bao,Zheng-Ying Liu,Ming-Bo Yang,Wei Yang
In chemical industry, elemental sulfur (S) is widely used and always causes environmental and pollution concerns. Hence, the rapid and simple detection of S has become an important issue. In this work, we report an effective approach for visible determination of S. The color of the detection reagent, which was readily prepared by mixing N, N-Dimethylformamide and hydrazine hydrate in a certain ratio, changes from colorless to blue and gradually fades after getting in contact with trace S. The detection reagent can be used for repeated S detection because of the color fading behavior. By measuring the characteristic absorption intensity during detection or the fading time of the mixture, the content of S in the sample can be readily determined and the detection limit can be as low as 0.32 mg/L. An unstable chromogenic organic clathrate generated during detection was found to be responsible for the chromogenic reaction, as revealed by UV–vis, FTIR, NMR, high resolution mass spectrometry measurements and simulation. Thus, this work presents a simple, fast and device-independent approach that can realize semi-quantitative detection of trace S in water, alcohol, food and environmental samples.
研究领域:
化学工业
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环境科学
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分析化学
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产品关联
DOI
高影响因子
Toxins
·
2020
·Vol. 12
·期 8
·521
·
IF 3.531
Xia Hong,Yuhao Mao,Chuqin Yang,Zhenjiang Liu,Ming Li,Daolin Du
Zearalenone (ZEN) is a prevalent mycotoxin that needs intensive monitoring. A semi-quantitative and quantitative immunochromatographic assay (ICA) was assembled for investigating ZEN contamination in 187 samples of cereal and their products from China in 2019. The semi-quantitative detection model had a limit of detection (LOD) of 0.50 ng/mL with visual judgment and could be completely inhibited within 5 min at 3.0 ng/mL ZEN. The quantitative detection model had a lower LOD of 0.25 ng/mL, and ZEN could be accurately and digitally detected from 0.25–4.0 ng/mL. The ICA method had a high sensitivity, specificity, and accuracy for on-site ZEN detection. For investigation of the authentic samples, the ZEN-positive rate was 62.6%, and the ZEN-positive levels ranged from 2.7 to 867.0 ng/g, with an average ZEN-positive level being 85.0 ng/g. Of the ZEN-positive samples, 6.0% exceeded the values of the limit levels. The ZEN-positive samples were confirmed to be highly correlated using LC-MS/MS (R2= 0.9794). This study could provide an efficiency and accuracy approach for ZEN in order to achieve visual and digitized on-site investigation. This significant information about the ZEN contamination levels might contribute to monitoring mycotoxin occurrence and for ensuring food safety.Keywords:zearalenone;immunochromatographic assay;semi-quantification;quantificationKey Contribution:A semi-quantitative and quantitative test strip had been developed and applied for investigating zearalenone contamination in 187 authentic samples in China in 2019.
研究领域:
食品安全
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分析化学
·
微生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2015
·Vol. 39
·期 2
·287-295
·
IF 2.737
Li Wang,Zhong Ping Yao,Ping Li,Si-Bao Chen,Pui-Kin So,Zi-Qi Shi,Bin Hu,Li-Fang Liu,Gui-Zhong Xin
Methods based on triple quadrupole tandem mass spectrometry have been widely used and reported as highly selective and sensitive methods for quantifying substances of herbal medicines. However, most of them were limited to targeted components, due to the difficulties to optimize the multiple reaction monitoring transitions without authentic standards. This study proposed a novel strategy for non-targeted optimization of multiple reaction monitoring method based on the diagnostic ion guided family classifications, tandem mass spectrometry database establishment, and transitions and collision energy screening. Applying this strategy, 59 Fritillaria alkaloids in Fritillariae Ussuriensis Bulbus have been classified, and 51 of these Fritillaria alkaloids were successfully detected by the optimal multiple reaction monitoring method. For semi-quantification, the easy-to-obtain Fritillaria alkaloids of each type, such as verticinone for cevanine type and peimisine for jervine type, were used as the reference standards to calibrate the other Fritillaria alkaloids in the same type. The method was demonstrated a good linearity ( R 2 > 0.998) with satisfactory accuracy and precision, and the lower limits of quantification of verticinone and peimisine were estimated to be 0.076 and 0.216 pg, respectively. In addition, the results suggested that the proposed strategy might obtained high quality metabolomics data in discrimination of Fritillaria unibracteata and Fritillaria ussuriensis .
研究领域:
中药分析
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质谱技术
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药用植物研究
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产品关联
DOI
PubMed
高影响因子
Journal of Cleaner Production
·
2022
·
IF 11.072
Xincheng Jiang,Yanyan An,Yaoyao Huang,Wei Ding,Manli Sun,Hong Li,Huaili Zheng
The residual antibiotics in the environment will pose a threat to the environment and human health. Thus, it is imperative to develop a smart water treatment material to remove and detect antibiotics from aqueous environment simultaneously. In this study, magnetic Cu–Ni–S@Ni foam was synthesized by liquid phase vulcanization method to in-situ load Cu–Ni–S nanostructures on the surface of Ni foam. The novelty of this material is the combination of adsorption-flocculation technology and semi-quantitative detection technology. Batch experiments showed that Cu–Ni–S@Ni foam has a high removal rate of norfloxacin (NOR) (96.92%), wide pH adaptability (pH = 4.0–10.0) and strong ion interference resistance (0.1–100 mmol/L). According to the Langmuir isotherm , the removal capacity can reach 417.4 mg/g at 25 °C, which is much higher than that of other water treatment agents reported in most studies. Characterization analysis indicated that the main removal mechanisms are surface complexation, cation bridging, electrostatic attraction , precipitation and flocculation . Transmission FTIR detection experiments showed that NOR on Cu–Ni–S@Ni foam has easily recognizable FTIR fingerprints; the intensity of characteristic peaks roughly reflects the concentration information of NOR to some extent. This semi-quantitative detection method, requiring simple operation without the fabrication of KBr tablet, has a wide linear range (5–100 mg/L) and a low limit of detection (4.6 mg/L). Therefore, Cu–Ni–S@Ni foam can be used for simultaneous removal and semi-quantitative detection of NOR in wastewater. It has potential application value in wastewater treatment and water quality monitoring.
研究领域:
环境科学
·
材料科学
·
水处理技术
✓ 已验证
产品关联
DOI
高影响因子
FUEL
·
2022
·
IF 8.035
Shuofan Li,Jianxun Wu,Yidi Wang,Yunyun Li,Weilai Zhang,Yahe Zhang,Kaiyuan He,Chuxuan Cai,Gangyue Bian,Hangzhou Wang,Ye Ji,Quan Shi
The developments of high-resolution mass spectrometry and soft ionization technologies have enabled the molecular characterization of petroleum fractions. However, quantitative analysis of molecular composition for petroleum is far from being achieved. In this study, semi-quantitative analysis of molecular composition for the whole compounds in heavy petroleum fractions was carried out. Hydrocarbons and various heteroatoms in the heavy petroleum fractions were characterized by only electrospray ionization coupled with high-resolution mass spectrometry. The molecular composition of various types of compounds were calculated and integrated quantitatively based on bulk chemical composition. The semi-quantitative molecular compositions of four heavy petroleum fractions with largely different bulk properties were comprehensively characterized in multiple dimensions such as carbon number distribution, condensation degree, molecular weight, mass fraction, and molecule number. More than 5,000 molecules, which classified into 16 compound classes of seven groups were quantitatively characterized from the four fractions. The accuracy of the analysis seems acceptable according the H/C ratio comparation between that derived from element analysis and the semi-quantitative molecular composition. Representative molecule set containing structural information was also proposed based on the semi-quantitative results.
研究领域:
分析化学
·
石油科学
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产品关联
DOI
高影响因子
Atmosphere
·
2019
·Vol. 10
·期 2
·88
·
IF 2.046
Rui Li,Xiaotong Jiang,Xinfeng Wang,Tianshu Chen,Lin Du,Likun Xue,Xinhui Bi,Mingjin Tang,Wenxing Wang
Semivolatile organic nitrates (SVONs) contribute a large proportion of total organic nitrates and play an important role in the tropospheric chemistry. However, the composition and concentrations of SVONs in the atmosphere remain unclear due to the lack of reliable analytical techniques for specific organic nitrates. In this study, a method based on gas chromatography and electron ionization–mass spectrometry was developed to detect ambient SVONs that were collected via polyurethane foam disk enrichment. Three SVONs were identified in the semivolatile samples from urban Jinan during spring based on the characteristic fragment ions of [NO2]+and [CH2NO3]+and the characteristic fragment loss of NO2and NO3: 1-pentyl nitrate (molecular weight [MW] = 133), 4-hydroxy-isoprene nitrate (MW = 147), and (3,4)-di-hydroxy-isoprene nitrate (MW = 163). The latter two isoprene nitrates were rarely detected in the real atmosphere in previous studies. The contents of 1-pentyl nitrate, 4-hydroxy-isoprene nitrate, and (3,4)-di-hydroxy-isoprene nitrate were roughly quantified based on the standard of 1-pentyl nitrate, with a detection limit of 50 μg L−1. In addition, Fourier transform infrared spectrometry was used to determine the total SVONs content. The average concentrations of 1-pentyl nitrate, 4-hydroxy-isoprene nitrate, (3,4)-di-hydroxy-isoprene nitrate, and total SVONs in Jinan during spring were 20.2 ± 7.2, 13.2 ± 7.2, 36.5 ± 8.4, and 380.0 ± 190.8 ng m−3, respectively. The three identified SVONs contributed only 20.2 ± 5.5% to the total SVONs, which suggests that some unidentified SVONs are present in the ambient atmosphere and that studies with improved or advanced analytical techniques will be required to identify them.Keywords:organic nitrates;semivolatile sample;GC/EI-MS;identification;semiquantification
研究领域:
大气科学
·
分析化学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Xiaoyue Yue,Yan Li,Sheng Xu,Junguang Li,Min Li,Liying Jiang,Mingsha Jie,Yanhong Bai
Developing intelligent, sensitive, and visual methods for rapidly detecting veterinary drug residues is essential for ensuring food quality and safety. A portable smartphone-assisted ratiometric fluorescent sensor was successfully designed using fluorescent Al-MOF nanosheet and rhodamine B (RhB) as fluorescent probes to adjust to the requirement of malachite green (MG) detection. The developed ratiometric fluorescent sensor allowed sensitive and selective detection of MG with good linear relationships in a wide range of 0.5–200 μg/mL. The Quantitative linear range is 5.3 μg/mL to 200 μg/mL. The limit of detection (LOD) and limit of quantification (LOQ) were calculated to be 1.6 μg/mL and 5.3 μg/mL respectively. The practicability of the proposed method was verified using high performance liquid chromatography (HPLC) in spiked fish tissues with satisfying recoveries and RSD. Moreover, portable smartphone-assisted fluorescent test papers were fabricated for the intelligent detection of MG. This integration of smartphones and fluorescent test papers was economical and saved time, providing an alternative strategy for the qualitative discernment and semi-quantitative analysis of MG on-site.
研究领域:
分析化学
·
食品科学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）